Aquatic Pollutants Tested in vitro with Early Passage Fish Cells
Keyword(s):
Epithelioid cells derived from fin tissues of bluegill sunfish fingerlings were used at early passage with the neutral red assay to assess the relative cytotoxicities of organochlorine pesticides. These fin cells were able to differentiate between the cytotoxicities of various test agents, although they were less sensitive than were the BF-2 cells, an established fibroblastic cell-line derived from the caudal trunk of bluegill fry. Both the fin epithelioid and the BF-2 fibroblast cells lacked significant xenobiotic metabolising capacity, based on determinations of 7-ethoxycoumarin O-deethylase as the indicator of monooxygenase activities. Such enzymatic activity could not be induced by exposure of the cultures to Arochlor 1254.