scholarly journals Comparative evaluation of a novel TaqMan real-time reverse transcription–polymerase chain reaction assay for hepatitis A virus detection

2013 ◽  
Vol 41 (2) ◽  
pp. 427-434 ◽  
Author(s):  
Feng Qiu ◽  
Huihui Zheng ◽  
Yao Yi ◽  
Zhiyuan Jia ◽  
Jingyuan Cao ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Polymerase Chain Reaction Assay ◽  
Comparative Evaluation ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Detection ◽  
Chain Reaction ◽  
Polymerase Chain

Persistence of Hepatitis A Virus in Oysters†

2003 ◽  
Vol 66 (2) ◽  
pp. 331-334 ◽  
Author(s):  
DAVID H. KINGSLEY ◽  
GARY P. RICHARDS
Keyword(s):  
Polymerase Chain Reaction ◽  
Crassostrea Virginica ◽  
Reverse Transcription ◽  
Hepatitis A ◽  
Infectious Virus ◽  
Hepatitis A Virus ◽  
Complete Removal ◽  
Chain Reaction ◽  
Daily Feeding ◽  
Polymerase Chain

We investigated the ability of hepatitis A virus (HAV) to persist for up to 6 weeks in Eastern oysters (Crassostrea virginica). Viral RNA was detected by reverse transcription–polymerase chain reaction 6 weeks after 16 h of exposure to 90,000 PFU (180 PFU/ml of seawater) of HAV. Assaying for infectious virus in oysters that received a daily feeding of phytoplankton recovered 3,800, 650, and 500 PFU of HAV 1, 2, and 3 weeks after contamination with 90,000 PFU of HAV, respectively. However, no infectious HAV was isolated from oysters 4, 5, or 6 weeks after contamination. These results support the position that shellfish depuration is insufficient for the complete removal of infectious viruses. Extended relay times (in excess of 4 weeks) may be required to produce virologically safe shellfish.

scholarly journals Reverse transcription-polymerase chain reaction assay for rabies virus detection

2004 ◽  
Vol 56 (3) ◽  
pp. 398-400 ◽  
Author(s):  
J.V. Dantas Junior ◽  
L.M.S. Kimura ◽  
M.S.R. Ferreira ◽  
A.M. Fialho ◽  
M.M.S. Almeida ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rabies Virus ◽  
Reverse Transcription ◽  
Polymerase Chain Reaction Assay ◽  
Virus Detection ◽  
Chain Reaction ◽  
Polymerase Chain
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