Effects of Hodgkin Cytotoxic Serum on Blast Transformation of Normal and Hodgkin Human Peripheral Blood Lymphocytes.

1975 ◽  
Vol 61 (1) ◽  
pp. 53-62 ◽  
Author(s):  
Sergio Tognella ◽  
Giovanni Mantovani ◽  
Gennaro S. Del Giacco ◽  
Paolo E. Manconi ◽  
Letizia Cengiarotti ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blast Transformation ◽  
Blood Lymphocytes ◽  
Cytotoxic Antibody ◽  
Human Peripheral Blood Lymphocytes ◽  
Before And After ◽  
Cytotoxic Serum

The PHA-resposiveness of normal and Hodgkin patient human peripheral blood lymphocytes has been studied before and after incubation with Hodgkin cytotoxic sera. The following conclusions have been reached: (a) Hodgkin cytotoxic serum is capable of decreasing the PHA-responsiveness of normal lymphocytes and of furtherly impairing the already defective PHA-responsiveness of Hodgkin lymphocytes. (b) The impaired PHA-responsiveness can be restored to the original levels by eluting the cytotoxic antibody. Control experiments in which normal and Hodgkin lymphocytes were put in contact with normal and Hodgkin non-cytotoxic serum showed no decrease of PHA-responsiveness. These data are in agreement with the hypothesis that the presence of serum cytotoxin is at least partly responsible for the immuno-incompetence of T-lymphocytes characteristic of Hodgkin's disease.

scholarly journals Effects of 2-amino-4,6-di-tert-butylphenol derivatives on the viability and functional state of human peripheral blood lymphocytes

2020 ◽  
pp. 19-28
Author(s):  
Darya B. Nizheharodava ◽  
Galina A. Ksendzova ◽  
Aliaksei G. Sysa ◽  
Mariya Yu. Yurkevich ◽  
Maryna V. Labai ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Immunomodulatory Activity ◽  
Peripheral Blood Mononuclear ◽  
Blood Lymphocytes ◽  
Tert Butyl ◽  
Human Peripheral Blood Lymphocytes

Derivatives of 2-amino-4,6-di-tert-butylphenol exhibit antiviral properties and radical regulatory activity against various types of organic radicals which determines the actuality of their further investigation. But the question of aminophenol derivatives immunomodulatory activity remains open. In this regard, the aim of the study was to assess the effects of 2-amino-4,6-di-tert-butylphenol derivatives on the viability and functional potential of human peripheral blood lymphocytes. As a result of the studies, it was shown that aminophenol compounds at concentrations of 10–5–10–7 mol did not exert a toxic effect while at a concentration of 10–4 mol showed a cytotoxic effect due to the induction of secondary necrosis. Compounds N-(2-hydroxy-3,5-di-tert-butylphenyl)-4-methylbenzenesulfonamide and 2,4-di-tert-butyl-6-morpholinophenol at a concentration of 10–6 mol stimulated the extracellular production of α-interferon by peripheral blood mononuclear cells and intracellular production of γ-interferon by CD3+T-lymphocytes. An immunosuppressive effect (more than 50 %) of N-(2-hydroxy-3,5-di-tert-butylphenyl)-4-methylbenzenesulfonamide and 2,4-di-tert-butyl-6-morpholinophenol compounds at a concentration of 10–5 mol was revealed to the mitogen-induced proliferation of T-lymphocytes.

scholarly journals Evaluation of Micronucleus and Nuclear Division Index in the Lymphocytes of some Iraqi Patients with Acute Lymphocyte Leukemia

2013 ◽  
Vol 7 (3) ◽  
pp. 43-53
Author(s):  
Wiaam A. Al Amili ◽  
Nuria Abdul Hussain ◽  
Abdul Hussain Al Faisal
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Lymphoblastic Leukemia ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Before And After ◽  
Nuclear Division Index ◽  
Males And Females

The aim of our study was to determine micronucleus MN frequency and nuclear division index in peripheral blood lymphocytes of patients diagnosed with Acute Lymphoblastic Leukemia ALL, who had undergone chemotherapy. Patients were treated with nine of drugs, which included Vincristine , Methotrexate , Cytosar-U, L-asparaginase , Etoposide, , dexamethasone (Decadron) , Indoxan , Steroids. The study was carried out on fifty Iraqi patients (34 Male, 16 Female), aged 2-70 years with Acute Lymphoblastic Leukemia ALL. These samples included 20 pretreatment aged 7-70 years, 15 under treatment aged 2-57years and 15 relapsed aged 9-40 years, compared with a sample consisted of 50 apparently healthy normal individuals collected randomly from population living in Baghdad aged 3-75 years. Results of the of MN in the human lymphocyte observed a significant increase p<0.05 in the males and females of human peripheral blood lymphocytes of patients with Acute Lymphoblastic Leukemia, before and after the chemotherapy as compared with the control. While, a significant decrease P> 0.05 in nucleic division index NDI was observed in the human peripheral blood lymphocytes of Acute Lymphoblastic Leukemia Patients (males and females), before and after the chemotherapy as compared with the control. In addition, the results of MN and NDI were compared in the genders of the groups studied. In conclusion, the results indicated that there is a possibility of using the changes in the mean of MN frequency and NDI as biomarkers for the assessment of DNA damage in the human peripheral blood lymphocytes of patients with Acute Lymphoblastic Leukemia ALL before and after the chemotherapy treatment and the increase frequencies of MN in ALL patients indicate the effect of antileukemic agents in inducing somatic genetic damage.

Effects of Hodgkin Cytotoxic Serum on Electrophoretic Mobility of Normal and Hodgkin Peripheral Blood Lymphocytes.

1975 ◽  
Vol 61 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Sergio Tognella ◽  
Giovanni Mantovani ◽  
Letizia Cengiarotti ◽  
Gennaro S. Del Giacco ◽  
Paolo E. Manconi ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Electrophoretic Mobility ◽  
Peripheral Blood ◽  
Hodgkin’S Disease ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Cytotoxic Antibody ◽  
Lymphocyte Surface ◽  
Cytotoxic Serum

The effect of Hodgkin patient cytotoxic sera on the electrophoretic mobility of normal and Hodgkin peripheral blood allo-lymphocytes has been studied. Contact with cytotoxic serum determined a significant decrease in the electrophoretic mobility of lymphocytes, due to the presence of cytotoxic antibody on the lymphocyte surface. The antibody seems to be directed against T-lymphocytes. The results are discussed in the light of the preceding data by the authors on the role of anti-T-autoantibodies in Hodgkin's disease.

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