Distinct Effects of Platelet-Rich Plasma and BMP13 on Rotator Cuff Tendon Injury Healing in a Rat Model

Most shoulder pain is related to muscle or tendon strain. Chronic pain is often triggered by prior injuries, especially if original injury was severe or was not allowed to heal completely. Shoulder injury can cause weakness, tenderness and loss of full joint mobility. More common and less traumatic causes include lifting, reaching and pulling move- ments that strain the muscles and tendons or sprain ligaments surrounding the shoulder joint. Injury may or may not be realized during the activity. Various conditions like shoulder joint impingement, frozen shoulder, and rotator cuff tendinitis have resembling symptoms under the umbrella of disease Avabahuka described in Ayurveda. Leech application was carried out in first stage where there is reduced vascular supply. Here a case report of a female aged 31years old, who had an acute on chronic rotator cuff strain from strenuous activity underwent three sittings of Jaloukavacarana. Keywords: Rotator cuff tendon injury, Avabahuka, Jaloukavacarana.

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Combined therapy with platelet rich plasma and basic fibroblast growth factor in the early healing of rotator cuff tear in a rat model

Journal of Shoulder and Elbow Surgery ◽

10.1016/j.jse.2019.09.003 ◽

2019 ◽

Vol 28 (11) ◽

pp. e389

Author(s):

Takeshi Kataoka ◽

Yutaka Mifune ◽

Atsuyuki Inui ◽

Takashi Kurosawa ◽

Kohei Yamaura ◽

...

Keyword(s):

Growth Factor ◽

Rotator Cuff ◽

Fibroblast Growth Factor ◽

Rotator Cuff Tear ◽

Rat Model ◽

Platelet Rich Plasma ◽

Combined Therapy ◽

Fibroblast Growth ◽

Cuff Tear ◽

Early Healing

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Platelet-rich plasma for enhancing surgical rotator cuff repair: evaluation and comparison of two application methods in a rat model

Archives of Orthopaedic and Trauma Surgery ◽

10.1007/s00402-013-1914-3 ◽

2013 ◽

Vol 134 (3) ◽

pp. 405-411 ◽

Cited By ~ 26

Author(s):

Ali Ersen ◽

M. Demirhan ◽

A. C. Atalar ◽

M. Kapicioğlu ◽

Gokhan Baysal

Keyword(s):

Rotator Cuff ◽

Rat Model ◽

Rotator Cuff Repair ◽

Platelet Rich Plasma ◽

Application Methods ◽

Cuff Repair

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Regeneration of a full-thickness defect of rotator cuff tendon with freshly thawed umbilical cord-derived mesenchymal stem cells in a rat model

Stem Cell Research & Therapy ◽

10.1186/s13287-020-01906-1 ◽

2020 ◽

Vol 11 (1) ◽

Author(s):

Ji-Hye Yea ◽

Jin-Kyung Park ◽

In Ja Kim ◽

Gayoung Sym ◽

Tae-Soo Bae ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Rotator Cuff ◽

Umbilical Cord ◽

Rat Model ◽

Animal Experiments ◽

Surrounding Tissue ◽

Control Group ◽

Full Thickness ◽

Rotator Cuff Tendon

Abstract Background It is difficult to immediately use mesenchymal stem cells (MSCs) for the patient with rotator cuff disease because isolation and culture time are required. Thus, the MSCs would be prepared in advanced in cryopreserved condition for an “off-the-shelf” usage in clinic. This study investigated the efficacy of freshly thawed MSCs on the regeneration of a full-thickness tendon defect (FTD) of rotator cuff tendon in a rat model. Methods We evaluated morphology, viability, and proliferation of cultured umbilical cord-derived MSCs (C-UC MSCs) and freshly thawed umbilical cord-derived MSCs (T-UC MSCs) at passage 10 in vitro. In animal experiments, we created a FTD in the supraspinatus of rats and injected the injured tendon with saline, cryopreserved agent (CPA; control), C-UC MSCs, and T-UC MSCs, respectively. Two and 4 weeks later, macroscopic, histological, biomechanical, and cell trafficking were evaluated. T test and ANOVA were used with SPSS. Differences with p < .05 were considered statistically significant. Results T-UC MSCs had fibroblast-like morphology and showed greater than 97% viability and stable proliferation comparable to the C-UC MSCs at passage 10. In animal experiments, compared with the control group, the macroscopic appearance of the T-UC MSCs was more recovered at 2 and 4 weeks such as inflammation, defect size, neighboring tendon, swelling/redness, the connecting surrounding tissue and slidability. Histologically, the nuclear aspect ratio, orientation angle of fibroblasts, collagen organization, and fiber coherence were improved by 33.33%, 42.75%, 1.86-fold, and 1.99-fold at 4 weeks, and GAG-rich area decreased by 88.13% and 94.70% at 2 and 4 weeks respectively. Further, the T-UC MSCs showed enhanced ultimate failure load by 1.55- and 1.25-fold compared with the control group at both 2 and 4 weeks. All the improved values of T-UC MSCs were comparable to those of C-UC MSCs. Moreover, T-UC MSCs remained 8.77% at 4 weeks after injury, and there was no significant difference between C-UC MSCs and T-UC MSCs. Conclusions The morphology, viability, and proliferation of T-UC MSCs were comparable to those of C-UC MSCs. Treatment with T-UC MSCs could induce tendon regeneration of FTD at the macroscopic, histological, and biomechanical levels comparable to treatment with C-UC MSCs.

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Regeneration of a Full-Thickness Defect of Rotator Cuff Tendon with Freshly Thawed Umbilical Cord-Derived Mesenchymal Stem Cells in a Rat Model

10.21203/rs.3.rs-34652/v1 ◽

2020 ◽

Author(s):

Ji-Hye Yea ◽

Jin-Kyung Park ◽

InJa Kim ◽

Gayoung Sym ◽

Tae Soo Bae ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Rotator Cuff ◽

Umbilical Cord ◽

Rat Model ◽

Animal Experiments ◽

Surrounding Tissue ◽

Control Group ◽

Full Thickness ◽

Rotator Cuff Tendon

Abstract Background: It is difficult to immediately use mesenchymal stem cells (MSCs) for the patient with rotator cuff disease because isolation and culture time are required. Thus, the MSCs would be prepared in advanced in cryopreserved condition for an “off-the-shelf” usage in clinic. This study investigated the efficacy of freshly thawed MSCs on the regeneration of a full-thickness tendon defect (FTD) of rotator cuff tendon in a rat model. Methods: We evaluated morphology, viability and proliferation of cultured umbilical cord-derived MSCs (C-UC MSCs) and freshly thawed umbilical cord-derived MSCs (T-UC MSCs) at passage 10 in vitro. In animal experiments, we created a FTD in the supraspinatus tendon of rats and injected the injured tendon with saline, cryopreserved agent (CPA; control), C-UC MSCs, and T-UC MSCs respectively. Two to four weeks later, macroscopic, histological and biomechanical changes were evaluated. T-test and ANOVA were used with SPSS. Differences with p < .05 were considered statistically significant.Results: T-UC MSCs had fibroblast-like morphology and showed greater than 97% viability and stable proliferation comparable to the C-UC MSCs at passage 10. In animal experiments, compared with the control group, the macroscopic appearance of the T-UC MSCs was further recovered at two and four weeks such as inflammation, defect size, neighboring tendon, swelling/redness and the connecting surrounding tissue and slidability. Histologically, compared to the control group the nuclear aspect ratio, orientation angle of fibroblasts, collagen organization and fiber coherence were improved by 33.33%, 42.75%, 1.86- and 1.99-fold and GAG-rich area was suppressed by 81.05% at four weeks. Further, the T-UC MSCs showed enhanced ultimate failure load by 1.55- and 1.25-fold compared with the control group at both two and four weeks. All the improved values of T-UC MSCs were comparable to those of C-UC MSCs. Conclusions: The morphology, viability and proliferation of T-UC MSCs are comparable to those of C-UC MSCs. Treatment with T-UC MSCs induces tendon regeneration of FTD at the macroscopic, histological and biomechanical levels comparable to treatment with C-UC MSCs.

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