Protective properties of five newly synthesized cyclic compounds against sodium azide and N-methyl-N′-nitro-N-nitrosoguanidine genotoxicity

2011 ◽  
Vol 28 (7) ◽  
pp. 605-613 ◽  
Author(s):  
Kadir Turhan ◽  
S Arda Ozturkcan ◽  
Zuhal Turgut ◽  
Mehmet Karadayi ◽  
Medine Gulluce
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Sodium Azide ◽  
Antimutagenic Activity ◽  
Bacterial Strains ◽  
Mutagenic Potential ◽  
Protective Properties ◽  
Genotoxic Agents ◽  
Cyclic Compounds ◽  
Chemical Prevention

The current study aims to determine the antimutagenic potential of five newly synthesized cyclic compounds against the genotoxic agents sodium azide (NaN3) and N-methyl- N′-nitro- N-nitrosoguanidine (MNNG). The mutant bacterial tester strains were NaN3-sensitive Salmonella typhimurium TA1535 and MNNG-sensitive Escherichia coli WP2 uvrA. According to the results, all the test compounds showed significant antimutagenic activity. The inhibition rates ranged from 26.05% (Compound 4—1 µg/plate) to 68.54% (Compound 5—0.01 µg/plate) for NaN3 and from 32.44% (Compound 3—1 µg/plate) to 60.77% (Compound 5—1 µg/plate) for MNNG genotoxicity. Moreover, the mutagenic potential of the test compounds was investigated using the same strains. The results showed that all the test compounds do not have mutagenic potential on the bacterial strains at the tested concentrations. Thus, the findings of the present study give valuable information about chemical prevention from NaN3 and MNNG genotoxicity.

Antimutagenic activity of vitamin B1 against damages induced by chemical and physical mutagens in Salmonella typhimurium and Escherichia coli

2017 ◽  
Vol 45 ◽  
pp. 202-206 ◽  
Author(s):  
Jaime Sánchez-Navarrete ◽  
Myriam Arriaga-Alba ◽  
Nancy Jannet Ruiz-Pérez ◽  
Julia Dolores Toscano-Garibay
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Vitamin B1 ◽  
Antimutagenic Activity

scholarly journals Testing of the mutagenic potential of N-(γ-aminobuturyl)-1-aza-15-crown-5 hydrochloride in Ames test microplate modification

2019 ◽  
pp. 81-87
Author(s):  
M. Ya. Golovenko ◽  
V. B. Larionov ◽  
S. S. Basok ◽  
A. S. Reder
Keyword(s):  
Salmonella Typhimurium ◽  
Sodium Azide ◽  
Anticonvulsant Activity ◽  
Ames Test ◽  
Point Mutations ◽  
Gene Mutations ◽  
Metabolic Activation ◽  
Chemical Mutagenesis ◽  
Mutagenic Potential ◽  
Significant Development

In recent years, studies in the field of chemical mutagenesis have undergone significant development, due to the introduction of a large number of different chemicals and scientific advances in the creation and use of new test systems, allowing a complete assessment of both mutagens themselves and their metabolites. The aim of the work was to determine possible induction of gene mutations under influence of hydrochloride N-(γ-aminobuturil)-1-aza-4,7,10,13-tetraozacyclopentadecan (TOCPD), which has nootropic, anxiolytic and anticonvulsant activity. The ability of TOCPD to induce gene mutations was evaluated in Ames test on strains Salmonella typhimurium ТА 98 (frame shift mutations) and ТА 100 (substitution point mutations). The compound was used at concentrations of 10, 100, 250, 500 and 1000 μg/ml. Standard mutagens were 2-nitrofluoren for Salmonella typhimurium ТА 98 and sodium azide for Salmonella typhimurium ТА 100 in test without metabolic activation. In an activation variant a microsomal activating mixture was used (S9 mix). In tests with activation for both strains, 2-aminoantracene was used. The µAmes kit, Moltox (USA) and Muta-ChromoPlate kit (Canada) were used in the work. The results were evaluated by the number of wells with mutated cells with medium color changing from purple to yellow. The obtained data showed that in the control and according to the action of corresponding mutagens, the percentage of wells with mutated cells corresponded to the standard parameters determined by protocol of the microplate test. For the action of TOCPD compound, no gene mutations were detected in both S. typhimurium ТА 98 and ТА 100 strains within the concentrations used.

Glutathione Deficiency does not Elevate Susceptibility of Bacteria to the Mutagenicity of Chlorinated Humic Acids

1994 ◽  
Vol 13 (8) ◽  
pp. 558-562 ◽  
Author(s):  
G.A. Ubom ◽  
J.K. Chipman ◽  
M.H.B. Hayes
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Humic Substances ◽  
Rat Liver ◽  
Humic Acids ◽  
Mutagenic Effect ◽  
Transferase Activity ◽  
Bacterial Strains ◽  
Glutathione S Transferase ◽  
Glutathione Deficiency

1 Rat liver 9,000 g supernatant protected against the mutagenic effect of chlorinated hydrophilic macromolecular humic acids (CHMA) in Salmonella typhimurium strain TA100. 2 Protection against mutagenicity of CHMA was mediated by glutathione and was partially dependent on glutathione S-transferase activity. 3 In contrast to the above findings, CHMA showed lower mutagenicity in Salmonella typhimurium and Escherichia coli strains of bacteria that are deficient in glutathione compared to their mutagenicity in parental (glutathione-rich) bacterial strains. 4 Glutathione-deficient cells do not provide test systems with elevated sensitivity for the detection of mutagenic chlorinated humic substances.

scholarly journals Deletion Formation Between the Two Salmonella typhimurium Flagellin Genes Encoded on the Mini F Plasmid: Escherichia coli ssb Alleles Enhance Deletion Rates and Change Hot-Spot Preference for Deletion Endpoints

Genetics ◽  
1997 ◽  
Vol 145 (3) ◽  
pp. 563-572 ◽  
Author(s):  
Takafumi Mukaihara ◽  
Masatoshi Enomoto
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Hot Spots ◽  
Hot Spot ◽  
Direct Repeats ◽  
Replication Slippage ◽  
Detection Systems ◽  
Deletion Formation ◽  
Homologous Sequences ◽  
Deletion Detection

Deletion formation between the 5′-mostly homologous sequences and between the 3′-homeologous sequences of the two Salmonella typhimurium flagellin genes was examined using plasmid-based deletion-detection systems in various Escherichia coli genetic backgrounds. Deletions in plasmid pLC103 occur between the 5′ sequences, but not between the 3′ sequences, in both RecA-independent and RecA-dependent ways. Because the former is predominant, deletion formation in a recA background depends on the length of homologous sequences between the two genes. Deletion rates were enhanced 30- to 50-fold by the mismatch repair defects, mutS, mutL and uvrD, and 250-fold by the ssb-3 allele, but the effect of the mismatch defects was canceled by the ΔrecA allele. Rates of the deletion between the 3′ sequences in plasmid pLC107 were enhanced 17- to 130-fold by ssb alleles, but not by other alleles. For deletions in pLC107, 96% of the endpoints in the recA+ background and 88% in ΔrecA were in the two hot spots of the 60- and 33-nucleotide (nt) homologous sequences, whereas in the ssb-3 background >50% of the endpoints were in four- to 14-nt direct repeats dispersed in the entire 3′ sequences. The deletion formation between the homeologous sequences is RecA-independent but depends on the length of consecutive homologies. The mutant ssb allele lowers this dependency and results in the increase in deletion rates. Roles of mutant SSB are discussed with relation to misalignment in replication slippage.

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