A comparative study of using free radical generators in the testing of chosen oxidative stress parameters in the different types of cells

The aim of this study was to assess whether there are differences between the results of determining oxidative stress markers obtained from different origin cell lines after exposure to chemicals generating free radicals. The studies considered two markers of oxidative stress: the level of thiobarbituric acid reactive substances (TBARS) and superoxide dismutase activity. The evaluation was performed in five cell lines: Chinese hamster ovary (CHO-9) cells, lung adenocarcinoma A549, macrophages RAW264.7, skin carcinoma cells A431, and keratinocytes HaCaT. Three compounds generating free radicals were used as a source of reactive oxygen/nitrogen: 2,2′-azobis-2-methyl-propanimidamide dihydrochloride (AAPH), sodium persulfate (SP), and 3-morpholinosydnonimine hydrochloride (SIN-1). The most appropriate cell line to assess the level of TBARS proved to be the murine macrophage cell line RAW 264.7. Equally, good performance was observed in the lung cancer cell line A549, but only when tested with AAPH and SP. In the case of measuring superoxide dismutase activity, it appeared that the most suitable cell line was also the RAW 264.7 line, although dispersion increased significantly at the highest concentrations of AAPH and SP measurements. When choosing a cell line to determine oxidative stress, the specificity of the stress-inducing compound and the parameter determined should be taken into consideration.

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Synthetic, Structural, and Anticancer Activity Evaluation Studies on Novel Pyrazolylnucleosides

Molecules ◽

10.3390/molecules24213922 ◽

2019 ◽

Vol 24 (21) ◽

pp. 3922

Author(s):

Yadav ◽

Sharma ◽

Kaushik ◽

Kumar ◽

Jha ◽

...

Keyword(s):

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Evaluation Studies ◽

Human Tumor ◽

Cancer Cell Line ◽

Significant Inhibition ◽

Lung Cancer Cell Line ◽

Anticancer Activities ◽

Nmr Studies

The synthesis of novel pyrazolylnucleosides 3a–e, 4a–e, 5a–e, and 6a–e are described. The structures of the regioisomers were elucidated by using extensive NMR studies. The pyrazolylnucleosides 5a–e and 6a–e were screened for anticancer activities on sixty human tumor cell lines. The compound 6e showed good activity against 39 cancer cell lines. In particular, it showed significant inhibition against the lung cancer cell line Hop-92 (GI50 9.3 µM) and breast cancer cell line HS 578T (GI50 3.0 µM).

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The Anticancer Peptide CIGB-552 Exerts Anti-Inflammatory and Anti-Angiogenic Effects through COMMD1

Molecules ◽

10.3390/molecules26010152 ◽

2020 ◽

Vol 26 (1) ◽

pp. 152

Author(s):

Hellen Daghero ◽

Julio Raúl Fernández Massó ◽

Soledad Astrada ◽

Maribel Guerra Vallespí ◽

Mariela Bollati-Fogolín

Keyword(s):

Cell Line ◽

Signaling Pathway ◽

Cell Lines ◽

Mechanism Of Action ◽

3D Cell Culture ◽

Cancer Cell Line ◽

Lung Cancer Cell Line ◽

Human Lung Cancer ◽

Knock Out ◽

Culture Models

CIGB-552 is a synthetic anti-tumor peptide capable of reducing tumor size and increasing the lifespan of tumor-bearing mice. Part of its anti-cancer effects consists of inducing apoptosis, modulating NF-kB signaling pathway, and the angiogenesis process. Although one of its major mediators, the COMMD1 protein, has been identified, the mechanism by which CIGB-552 exerts such effects remains elusive. In the present study, we show the role of COMMD1 in CIGB-552 mechanism of action by generating the COMMD1 knock-out from the human lung cancer cell line NCI-H460. A microarray was performed to analyze both wild-type and KO cell lines with regard to CIGB-552 treatment. Additionally, different signaling pathways were studied in both cell lines to validate the results. Furthermore, the interaction between CIGB-552 and COMMD1 was analyzed by confocal microscopy. By signaling pathway analysis we found that genes involved in cell proliferation and apoptosis, oncogenic transformation, angiogenesis and inflammatory response are potentially regulated by the treatment with CIGB-552. We then demonstrated that CIGB-552 is capable of modulating NF-kB in both 2D and 3D cell culture models. Finally, we show that the ability of CIGB-552 to negatively modulate NF-kB and HIF-1 pathways is impaired in the COMMD1 knock-out NCI-H460 cell line, confirming that COMMD1 is essential for the peptide mechanism of action.

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In vitro Cytotoxic Activity of Sesamin Isolated from Vismia baccifera var. dealbata Triana & Planch (Guttiferae) Collected from Venezuela

Natural Product Communications ◽

10.1177/1934578x0800301025 ◽

2008 ◽

Vol 3 (10) ◽

pp. 1934578X0800301 ◽

Cited By ~ 2

Author(s):

Fabiola Salas ◽

Janne Rojas ◽

Antonio Morales ◽

Maria E. Ramos-Nino ◽

Nelida G. Colmenares

Keyword(s):

Lung Cancer ◽

Cytotoxic Activity ◽

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Line ◽

Lung Cancer Cell Line ◽

Cytostatic Activity ◽

Lung Cancer Cell

Sesamin extracted from Vismia baccifera var. dealbata was demonstrated to have cytostatic activity on the cancer cell lines tested, particularly the lung cancer cell line, with an IC50 of 1 g/L.

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Effect of cow colostrum, mare milk, and human milk on the viability of lung healthy and cancer cell lines

10.32592/ircmj.2021.23.5.409 ◽

2021 ◽

Keyword(s):

Lung Cancer ◽

Human Milk ◽

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Line ◽

Cancer Cell Lines ◽

Lung Cancer Cell Line ◽

Lung Cancer Cell ◽

Healthy Lung

This study investigated the effects of lyophilized mare milk, human milk, and cow colostrum on both human lung cancer cell lines, called A549, and healthy lung cell lines, called MRC5. Mare milk, human milk, and cow colostrum varieties were applied to 6 replicates in both cell lines with lyophilized milk concentrations ranging from 50-3,200 ppm. The cell viability was monitored by optic microscopy and determined by the MTT test. ANOVA and Duncan's multiple range tests were used to analyze data. The results of this study indicated that the most effective milk type on reducing the A549 lung cancer cell line was human milk, followed by mare milk; however, cow colostrum showed little effect. It was observed that human milk and mare milk had anti-proliferative effects on lung cancer cell line at concentrations which were non-toxic to healthy lung cell line.

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3,4,5-Trihydroxybenzoic Acid Triggered Oxidative Stress in 95-D Lung Cancer Cell Line

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.926-930.1061 ◽

2014 ◽

Vol 926-930 ◽

pp. 1061-1064

Author(s):

Yan Li Xi ◽

Xiang Qun Wu ◽

Jie Yu ◽

Wei Guo Xu ◽

Tong Zhao ◽

...

Keyword(s):

Oxidative Stress ◽

Lung Cancer ◽

Cancer Cells ◽

Cell Line ◽

Cancer Cell ◽

Cancer Cell Line ◽

Lung Cancer Cell Line ◽

Human Lung Cancer ◽

Dependent Manner ◽

Lung Cancer Cell

It is a good therapeutic method that add exogenous ROS to trigger oxidative stress causing death of cancer cells. In the present study, we investigated the inhibitory effects of 3,4,5-trihydroxybenzoic acid (TBA), a polyhydroxyphenolic compound, on high metastatic human lung cancer cell line (95-D) based on inducing reactive oxygen species (ROS). The experiments in vitro showed that 95-D cell viability was inhibited by various amounts of TBA and death was induced in a dose-dependent manner. The possible mechanism was that TBA can induce cell death by decreasing mitochondrial membrane potential (MMP; ΔΨm) and increasing hydrogen peroxide (H2O2) level. These results imply that TBA efficiently induces death in 95-D lung cancer cells and that TBA exerts cytotoxicity on cancer cells by its pro-oxidative activity.

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