Incorporation of salmon calcitonin-loaded poly(lactide-co-glycolide) (PLGA) microspheres into calcium phosphate bone cement and the biocompatibility evaluation in vitro

Crab shells are waste product, rich with calcium compound. Calcium element is often used as a material for bone scaffold due to its bioactive and biodegradation properties. In this study, calcium phosphate (CaP) nanoparticles were synthesized from crab shells through a wet chemical route. The CaP nanoparticles were then sintered and mixed with poly(lactic-co-glycolic acid) (PLGA) to form a bone cement composite. The mixture was casted in a cylinder shape and it was characterized through ATR-FTIR, XRD, FESEM, contact angle and DSC analyses. The CaP pellet and the CaP/PLGA bone cement composite were then subjected to in vitro simulated body fluid (SBF) bioactivity test. The CaP/PLGA bone cement composite was found to have a composition of crystal CaP and PLGA with a tolerable glass transition state, suitable to be used in a physiological environment. The CaP nanoparticles were agglomerated on the 3D interconnected surface of PLGA. The hydrophobicity of the CaP was increased (66.94%) with the addition of PLGA as a binder matrix where this composite has induced the formation of apatite layer. This bioactive property is crucial in fabricating a bone substitute material as it can promotes cell penetration, attachment and proliferation..

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Preliminary Study on the Biocompatibility of Calcium Phosphate Bone Cement Containing N,O-Carboxymethyl Chitosan

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.330-332.807 ◽

2007 ◽

Vol 330-332 ◽

pp. 807-810

Author(s):

Qi Huang ◽

Feng Cao ◽

Dong Xu Li

Keyword(s):

Calcium Phosphate ◽

Bone Cement ◽

Normal Saline ◽

Carboxymethyl Chitosan ◽

In Vitro Tests ◽

Calcium Phosphate Bone Cement ◽

Biological Reaction ◽

Good Biocompatibility ◽

Preliminary Study

N,O-carboxymethyl chitosan (CMCTS) was added in Calcium phosphate bone cement (CPC). A preliminary study was carried out in order to evaluate the biocompatibility of CPC containing CMCTS. In vitro tests were done using extract liquid from normal saline. The result showed that the biological reaction complied with standards of GB/16886 and the composite might have good biocompatibility.

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In vitro and in vivo effectiveness of a novel injectable calcitonin-loaded collagen/ceramic bone substitute

Journal of Biomaterials Applications ◽

10.1177/0885328221989984 ◽

2021 ◽

pp. 088532822198998

Author(s):

Karl Wu ◽

Yu-Chun Chen ◽

Shang M Lin ◽

Chih-Hung Chang

Keyword(s):

Calcium Phosphate ◽

Bone Cement ◽

Degradation Rate ◽

Rabbit Model ◽

Type I ◽

Calcium Phosphate Bone Cement ◽

Osteogenic Effect ◽

Composite Bone

This study aimed to evaluate the effectiveness of a novel calcitonin-loaded calcium phosphate composite bone cement in vitro and in vivo. The novel composite bone cements were composed of NuROs injectable bone graft substitute, type I collagen, and/or salmon calcitonin. The setting time, porosity, wettability, compressive strength, compressive modulus, and crystallographic structures of cement specimens were determined. Degradation rate, calcitonin release rate, and osteoinductivity were assessed in vitro. In addition, osteogenic effect was examined in a rabbit model of femoral defect. The results revealed that addition of collagen/calcitonin did not substantially alter physical properties and degradation rate of bone cement specimens. Calcitonin was released into culture medium in a two-phase manner. Osteogenic effect of conditioned medium derived from calcitonin containing bone cement was observed. Finally, de novo bone growth and bone mineralization across the bone defect area were observed in rabbits after implantation of composite bone cement specimens. In conclusion, this novel calcitonin-loaded composite calcium phosphate bone cement exhibits biocompatibility, bioresorbability, osteoinductivity, and osteoconductivity, which may be suitable for clinical use.

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A novel strontium(II)-modified calcium phosphate bone cement stimulates human-bone-marrow-derived mesenchymal stem cell proliferation and osteogenic differentiation in vitro

Acta Biomaterialia ◽

10.1016/j.actbio.2013.07.027 ◽

2013 ◽

Vol 9 (12) ◽

pp. 9547-9557 ◽

Cited By ~ 132

Author(s):

M. Schumacher ◽

A. Lode ◽

A. Helth ◽

M. Gelinsky

Keyword(s):

Bone Marrow ◽

Cell Proliferation ◽

Stem Cell ◽

Calcium Phosphate ◽

Mesenchymal Stem Cell ◽

Bone Cement ◽

Osteogenic Differentiation ◽

Stem Cell Proliferation ◽

Calcium Phosphate Bone Cement

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Improving osteogenesis of calcium phosphate bone cement by incorporating with lysine: An in vitro study

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2019.02.034 ◽

2019 ◽

Vol 177 ◽

pp. 462-469 ◽

Cited By ~ 3

Author(s):

Haishan Shi ◽

Xiaoling Ye ◽

Fupo He ◽

Jiandong Ye

Keyword(s):

Calcium Phosphate ◽

Bone Cement ◽

In Vitro Study ◽

Vitro Study ◽

Calcium Phosphate Bone Cement

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Modulation of Alendronate release from a calcium phosphate bone cement: An in vitro osteoblast-osteoclast co-culture study

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2018.11.023 ◽

2019 ◽

Vol 554 ◽

pp. 245-255 ◽

Cited By ~ 8

Author(s):

Luisa Stella Dolci ◽

Silvia Panzavolta ◽

Paola Torricelli ◽

Beatrice Albertini ◽

Laura Sicuro ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Cement ◽

Culture Study ◽

Calcium Phosphate Bone Cement

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Preparation and In Vitro Releasing of Salmon Calcitonin Carried Porous Injectable Calcium Phosphate Bone Cement

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.865 ◽

2006 ◽

Vol 309-311 ◽

pp. 865-868

Author(s):

Dong Xiao Li ◽

Q. Yao ◽

Hong Song Fan ◽

Ji Yong Chen ◽

Yu Mei Xiao ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Defect ◽

Salmon Calcitonin ◽

Bone Repair ◽

Bone Growth ◽

Uniform Design ◽

Deionized Water ◽

Rabbit Serum ◽

Calcium Phosphate Bone Cement

Calcium phosphate cement (CPC) has many advantages and is frequently used as a carrier of antibiotic and bone growth factors. Salmon calcitonin(S-CT) is effective in treating osteoporosis. Due to its potential of promoting bone cell proliferation, S-CT was combined with injectable CPC to accelerate the restoring of the bone defect induced by osteoporosis and the composite can be used in minimal invasive surgery. Uniform design was used to optimize the prescription, and the effects of pore-maker and S-CT on the characters of CPC were studied. The in vitro releasing of S-CT from the optimum CPC in deionized water, SBF and rabbit serum was studied with HPLC, respectively. The results showed that the contents of HA and citric acid-NaHCO3 affect the physical characters of CPC sharply, but S-CT has little effect on it. The releasing of S-CT in three different medium follow Hugichi equation, but the speed is slower in rabbit serum than in deionized water and SBF. S-CT carried porous injectable CPC composite possesses the basic performance for clinical needs, and it is promising to be used in osteoporosis induced bone defect and accelerate bone repair.

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