Enzyme-Linked Immunosorbent Assay Gliadin Assessment in Processed Food Products Available for Persons With Celiac Disease

2011 ◽  
Vol 26 (6) ◽  
pp. 695-699 ◽  
Author(s):  
Charalampos Agakidis ◽  
Thomais Karagiozoglou-Lampoudi ◽  
Marina Kalaitsidou ◽  
Theodoros Papadopoulos ◽  
Afroditi Savvidou ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Immunosorbent Assay ◽  
Food Products ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Food

scholarly journals Evaluation of the INOVA Diagnostics Enzyme-Linked Immunosorbent Assay Kits for Measuring Serum Immunoglobulin G (IgG) and IgA to Deamidated Gliadin Peptides

2006 ◽  
Vol 13 (1) ◽  
pp. 150-151 ◽  
Author(s):  
Harry E. Prince
Keyword(s):  
Celiac Disease ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Immunoglobulin A ◽  
Serum Immunoglobulin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gliadin Antibodies ◽  
Gliadin Peptides ◽  
Disease Specific ◽  
Inova Diagnostics

ABSTRACT New assays for antibodies to deamidated gliadin peptides (DGP) expressing celiac disease-specific epitopes were evaluated using 154 sera previously tested for endomysial immunoglobulin A (IgA) (EMA), transglutaminase IgA (TGA), and conventional gliadin antibodies. DGP antibody results showed 97% concordance with EMA and TGA results. Of 56 sera negative for EMA and TGA but positive for conventional gliadin antibodies, 54 (96%) were negative for DGP antibodies.

Antigliadin antibodies detected by enzyme-linked immunosorbent assay as a marker of childhood celiac disease

1988 ◽  
Vol 113 (2) ◽  
pp. 286-289 ◽  
Author(s):  
Neil T. Tucker ◽  
Fikry S. Barghuthy ◽  
Thomas J. Prihoda ◽  
Vijay Kumar ◽  
Aaron Lerner ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigliadin Antibodies

Enzyme-Linked Immunosorbent Assay Detection of Melamine in Infant Formula and Wheat Food Products

2010 ◽  
Vol 73 (4) ◽  
pp. 701-707 ◽  
Author(s):  
ERIC A. E. GARBER ◽  
VICKERY A. BREWER
Keyword(s):  
Sample Preparation ◽  
Infant Formula ◽  
Immunosorbent Assay ◽  
Cyanuric Acid ◽  
Food Products ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Limits Of Detection ◽  
Wheat Products ◽  
Assay Detection

The adulteration of food products with melamine to inflate the nitrogen content necessitates the establishment of analytical methods that can distinguish between proteinaceous ingredients and such adulterants. The specificity and ability to detect melamine by two commercial enzyme-linked immunosorbent assay (ELISA) kits were evaluated along with three protocols for sample preparation. Both ELISAs displayed cross-reactivity with ammeline, but neither was able to detect ammelide or cyanuric acid, indicating either a requirement for the 4,6-diamino-1,3,5-triazine structure or inability to bind 1,3,5-triazine-4,6-diones. The limits of detection for melamine in powder infant formula ranged from 0.2 to 3 μg/g depending on the ELISA kit and the method used to prepare the sample. The limits of detection for melamine in liquid infant formula and wheat products were <1 μg/ml and <2.5 μg/g, respectively. The ELISA kits provide an effective alternative for the analysis of samples suspected of containing melamine without relying on extensive sample preparation or expensive instrumentation.

scholarly journals Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease

1998 ◽  
Vol 114 ◽  
pp. A420 ◽  
Author(s):  
S. Sulkanen ◽  
T. Halttunen ◽  
I. Korponay-Szabo ◽  
K. Laurila ◽  
P. Collin ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Immunosorbent Assay ◽  
Tissue Transglutaminase ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Interlaboratory Evaluation of Two Enzyme-Linked Immunosorbent Assay Kits for the Detection of Egg, Milk, Wheat, Buckwheat, and Peanut in Foods

2006 ◽  
Vol 89 (6) ◽  
pp. 1600-1608 ◽  
Author(s):  
Rieko Matsuda ◽  
Yasuo Yoshioka ◽  
Hiroshi Akiyama ◽  
Kenichi Aburatani ◽  
Yumiko Watanabe ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Extraction Efficiency ◽  
Interlaboratory Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Screening Methods ◽  
Good Reproducibility ◽  
Processed Food ◽  
Elisa Kit ◽  
Denatured Proteins ◽  
Extraction Buffer

Abstract The labeling of 5 major allergenic ingredients (egg, milk, wheat, buckwheat, and peanut) is mandatory in Japan, and 2 series of enzyme-linked immunosorbent assay (ELISA) kits have been established as official screening methods. However, these official methods have not provided the necessary sensitivity, due in part to poor extraction efficiency. To address this need, 2 novel ELISA kits have been developed: the FASTKIT ELISA Ver. II Series and the FASPEK Allergenic Substances Detection Kit. The new kit systems use an improved extraction buffer that can extract insoluble proteins produced by processing and feature new antibodies that bind to the denatured proteins extracted with the new extraction buffer. The analytical performances of the 2 new ELISA kit series were evaluated in an interlaboratory study. Ten laboratories participated in the study and determined the major allergenic ingredients contained in 5 types of model processed food. The 2 ELISAs displayed fairly good reproducibility and sufficient recovery.

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