Development of a Sandwich Enzyme-linked Immunosorbent Assay (ELISA) for the Detection of Egg Residues in Processed Food Products

2021 ◽  
Author(s):  
Jin Chen ◽  
Hong Lin ◽  
Siyue Li ◽  
Jinlong Zhao ◽  
Ishfaq Ahmed ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Food Products ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Food

Enzyme-Linked Immunosorbent Assay Gliadin Assessment in Processed Food Products Available for Persons With Celiac Disease

2011 ◽  
Vol 26 (6) ◽  
pp. 695-699 ◽  
Author(s):  
Charalampos Agakidis ◽  
Thomais Karagiozoglou-Lampoudi ◽  
Marina Kalaitsidou ◽  
Theodoros Papadopoulos ◽  
Afroditi Savvidou ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Immunosorbent Assay ◽  
Food Products ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Food

Enzyme-Linked Immunosorbent Assay Detection of Melamine in Infant Formula and Wheat Food Products

2010 ◽  
Vol 73 (4) ◽  
pp. 701-707 ◽  
Author(s):  
ERIC A. E. GARBER ◽  
VICKERY A. BREWER
Keyword(s):  
Sample Preparation ◽  
Infant Formula ◽  
Immunosorbent Assay ◽  
Cyanuric Acid ◽  
Food Products ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Limits Of Detection ◽  
Wheat Products ◽  
Assay Detection

The adulteration of food products with melamine to inflate the nitrogen content necessitates the establishment of analytical methods that can distinguish between proteinaceous ingredients and such adulterants. The specificity and ability to detect melamine by two commercial enzyme-linked immunosorbent assay (ELISA) kits were evaluated along with three protocols for sample preparation. Both ELISAs displayed cross-reactivity with ammeline, but neither was able to detect ammelide or cyanuric acid, indicating either a requirement for the 4,6-diamino-1,3,5-triazine structure or inability to bind 1,3,5-triazine-4,6-diones. The limits of detection for melamine in powder infant formula ranged from 0.2 to 3 μg/g depending on the ELISA kit and the method used to prepare the sample. The limits of detection for melamine in liquid infant formula and wheat products were <1 μg/ml and <2.5 μg/g, respectively. The ELISA kits provide an effective alternative for the analysis of samples suspected of containing melamine without relying on extensive sample preparation or expensive instrumentation.

scholarly journals Interlaboratory Evaluation of Two Enzyme-Linked Immunosorbent Assay Kits for the Detection of Egg, Milk, Wheat, Buckwheat, and Peanut in Foods

2006 ◽  
Vol 89 (6) ◽  
pp. 1600-1608 ◽  
Author(s):  
Rieko Matsuda ◽  
Yasuo Yoshioka ◽  
Hiroshi Akiyama ◽  
Kenichi Aburatani ◽  
Yumiko Watanabe ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Extraction Efficiency ◽  
Interlaboratory Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Screening Methods ◽  
Good Reproducibility ◽  
Processed Food ◽  
Elisa Kit ◽  
Denatured Proteins ◽  
Extraction Buffer

Abstract The labeling of 5 major allergenic ingredients (egg, milk, wheat, buckwheat, and peanut) is mandatory in Japan, and 2 series of enzyme-linked immunosorbent assay (ELISA) kits have been established as official screening methods. However, these official methods have not provided the necessary sensitivity, due in part to poor extraction efficiency. To address this need, 2 novel ELISA kits have been developed: the FASTKIT ELISA Ver. II Series and the FASPEK Allergenic Substances Detection Kit. The new kit systems use an improved extraction buffer that can extract insoluble proteins produced by processing and feature new antibodies that bind to the denatured proteins extracted with the new extraction buffer. The analytical performances of the 2 new ELISA kit series were evaluated in an interlaboratory study. Ten laboratories participated in the study and determined the major allergenic ingredients contained in 5 types of model processed food. The 2 ELISAs displayed fairly good reproducibility and sufficient recovery.

ELISA Survey of Retail Grain-Based Food Products for Zearalenone and Aflatoxin B1

1987 ◽  
Vol 50 (6) ◽  
pp. 502-503 ◽  
Author(s):  
ROSCOE L. WARNER ◽  
JAMES J. PESTKA
Keyword(s):  
Wheat Flour ◽  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Food Products ◽  
Average Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Corn Meal ◽  
Breakfast Cereal ◽  
Snack Foods ◽  
Retail Grocery

Seventy-nine grain-based food products were purchased from mid-Michigan retail grocery outlets in 1985 and analyzed for the mycotoxins zearalenone and aflatoxin B1 by enzyme-linked immunosorbent assay. Twenty-two percent of these samples contained detectable zearalenone (limit ⩾2.5 μg/kg). Zearalenone was found in breakfast cereal, snack foods, popcorn, corn meal, and cake-muffin mixes representing 10, 11, 57, 78, and 20% of these samples, respectively. The average level of this toxin among the positive samples was 20 μg/kg with maximum levels of 120 and 130 μg/kg being found in samples of corn meal and popcorn, respectively. Zearalenone was not found in any of the wheat flour or baby foods samples. Detectable aflatoxin B1 (limit ⩾5.0 μg/kg) was not found in any of the 79 samples tested.

scholarly journals Study on the Detection of Prion Protein in Food Products by a Competitive Enzyme-linked Immunosorbent Assay.

2002 ◽  
Vol 43 (3) ◽  
pp. 173-177 ◽  
Author(s):  
Kaori TAKEKIDA ◽  
Yutaka KIKUCHI ◽  
Takeshi YAMAZAKI ◽  
Tomoshi KAKEYA ◽  
Kosuke TAKATORI ◽  
...  
Keyword(s):  
Prion Protein ◽  
Immunosorbent Assay ◽  
Food Products ◽  
Enzyme Linked Immunosorbent Assay

Development of a Chemiluminescence ELISA for the Detection of Sudan I in Chilli Powder

2013 ◽  
Vol 830 ◽  
pp. 310-313
Author(s):  
Yan Fan ◽  
Li Xin Zhu ◽  
Ren Rong Liu ◽  
Long Xu ◽  
Wei Meng
Keyword(s):  
Immunosorbent Assay ◽  
Recovery Rate ◽  
Limit Of Detection ◽  
Food Products ◽  
Food Samples ◽  
Linear Range ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sudan I ◽  
Antigen Antibody

A chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) was developed for the detection of Sudan I in food products. The CL-ELISA conditions, such as the concentration of antigen, antibody and goat anti-mouse IgG-HRP, were optimized. The optimized CL-ELISA allowed the Sudan I detection in a linear range of 0.625-10 ng mL-1, the IC50 was 3.3ng mL-1 and the limit of detection (LOD) was 0.31 ng mL-1. The method showed good recoveries with spiked chilli powder. The recovery rate in a batch range from 73-109.6%, and the recovery rate between the batch range from 78-109.24%. The proposed method proved to be efficient for the detection of Sudan I in food samples.

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