Effect of Fusidic Acid on Pancreatic Islet Allograft Rejection

1997 ◽  
Vol 6 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Josá F. Mendola ◽  
Helena Corominola ◽  
Enric Esmatjes ◽  
Alex Saenz ◽  
Laureano Fernandez-Cruz ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Fusidic Acid ◽  
Allograft Rejection ◽  
Diabetic Rats ◽  
Allograft Survival ◽  
Islet Allograft ◽  
Islet Allografts ◽  
Acinar Tissue

We examined in fully mismatched rats, the survival of pancreatic islet allografts in recipients treated with either fusidic acid (FA), an antistaphyllococcal antibiotic that has been shown to possess an immunosuppressive effect in vitro and in vivo, or cyclosporin-A (CsA). Islets were isolated by collagenase digestion, separated from acinar tissue by handpicking under a dissecting microscope and transplanted into the liver by portal vein injection of streptozotocin(STZ)-induced diabetic rats. The results indicated that while a temporary immunosuppression with CsA achieved an indefinite islet allograft survival, FA administered to recipients daily was not able to prevent islet allograft rejection across a major histocompatibility barrier. We conclude that despite the fact that fusidic acid has been claimed to act as an immunosuppressant drug in vitro with effects similar to those of CsA, unlike CsA, FA given either orally or by s.c. injection was not effective to prolong islet allograft survival in vivo. Copyright © 1997 Elsevier Science Inc.

Effect of cyclosporine A treatment in vitro on pancreatic islet allograft rejection

1997 ◽  
Vol 29 (5) ◽  
pp. 2494-2497
Author(s):  
J. Mendola ◽  
H. Corominola ◽  
E. Esmatjes ◽  
A. Saenz ◽  
L. Fernandez-Cruz ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Cyclosporine A ◽  
Allograft Rejection ◽  
Islet Allograft ◽  
Pancreatic Islet Allograft

Diazoxide pretreatment improves pancreatic islet survival in vitro and functionality in vivo

2018 ◽  
Author(s):  
Michiel Nijhoff ◽  
Francoise Carlotti ◽  
Marten Engelse ◽  
Koning Eelco de
Keyword(s):  
Pancreatic Islet ◽  
Islet Survival

Promotion of pancreatic islet allograft survival by intrathymic transplantation of bone marrow

Diabetes ◽  
1992 ◽  
Vol 41 (6) ◽  
pp. 771-775 ◽  
Author(s):  
A. M. Posselt ◽  
J. S. Odorico ◽  
C. F. Barker ◽  
A. Naji
Keyword(s):  
Bone Marrow ◽  
Pancreatic Islet ◽  
Allograft Survival ◽  
Islet Allograft ◽  
Pancreatic Islet Allograft

scholarly journals Amelioration of lipid peroxidation in vivo and in vitro by Satureja khozestanica essential oil in alloxan-induced diabetic rats

2014 ◽  
Vol 13 (1) ◽  
Author(s):  
Hassan Ahmadvand ◽  
Majid Tavafi ◽  
Ali Khosrowbeygi ◽  
Gholamreza Shahsavari ◽  
Maryam Hormozi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Essential Oil ◽  
Diabetic Rats

Reepithelialization of Orthotopic Tracheal Allografts Prevents Rejection after Withdrawal of Immunosuppression

2005 ◽  
Vol 114 (4) ◽  
pp. 279-288 ◽  
Author(s):  
Satish Govindaraj ◽  
Elena Fedorova ◽  
Eric M. Genden ◽  
Houtan Chaboki ◽  
Jonathan S. Bromberg ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cyclosporine A ◽  
Allograft Rejection ◽  
Lymphocyte Subpopulation ◽  
Ciliated Epithelium ◽  
Prior Work ◽  
Cellular Infiltrate ◽  
Tracheal Transplantation

Prior work has demonstrated that immunosuppressed orthotopic tracheal allografts undergo progressive reepithelialization over a 48-day period with recipient-derived tracheal epithelium. We hypothesized that reepithelialization of tracheal allografts would prevent rejection after withdrawal of immunosuppression. BALB/c murine tracheal grafts were transplanted orthotopically into either syngeneic or allogeneic C57/BL6 recipients. The recipients were either not immunosuppressed, immunosuppressed with cyclosporine A (10 mg/kg per day) continuously, or immunosuppressed for 48 days and then withdrawn from immunosuppression. The grafts were assessed for acute and chronic rejection 10 days and 50 days after immunosuppression withdrawal. The immunosuppressed allograft recipients maintained a ciliated epithelium acutely and chronically after immunosuppression withdrawal. Ten days after immunosuppression withdrawal, there was a mild cellular infiltrate, which resolved 50 days after withdrawal. Electron microscopy, lymphocyte subpopulation assays, and lamina propria analysis demonstrated that immunosuppression withdrawal did not result in tracheal allograft rejection. In vitro and in vivo assessments did not demonstrate evidence of systemic or local immune tolerance. We conclude that reepithelialization of orthotopic tracheal allografts with recipient-derived mucosa prevents rejection of allograft segments. Tracheal transplantation may require only transient immunosuppression, which can be withdrawn after tracheal reepithelialization.

Sequoyitol ameliorates diabetic nephropathy in diabetic rats induced with a high-fat diet and a low dose of streptozotocin

2014 ◽  
Vol 92 (5) ◽  
pp. 405-417 ◽  
Author(s):  
Xian-Wei Li ◽  
Yan Liu ◽  
Wei Hao ◽  
Jie-Ren Yang
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Glucose ◽  
High Fat Diet ◽  
Low Dose ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
High Fat

Sequoyitol decreases blood glucose, improves glucose intolerance, and enhances insulin signaling in ob/ob mice. The aim of this study was to investigate the effects of sequoyitol on diabetic nephropathy in rats with type 2 diabetes mellitus and the mechanism of action. Diabetic rats, induced with a high-fat diet and a low dose of streptozotocin, and were administered sequoyitol (12.5, 25.0, and 50.0 mg·(kg body mass)−1·d−1) for 6 weeks. The levels of fasting blood glucose (FBG), serum insulin, blood urea nitrogen (BUN), and serum creatinine (SCr) were measured. The expression levels of p22phox, p47phox, NF-κB, and TGF-β1 were measured using immunohistochemisty, real-time PCR, and (or) Western blot. The total antioxidative capacity (T-AOC), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were also determined. The results showed that sequoyitol significantly decreased FBG, BUN, and SCr levels, and increased the insulin levels in diabetic rats. The level of T-AOC was significantly increased, while ROS and MDA levels and the expression of p22phox, p47phox, NF-κB, and TGF-β1 were decreased with sequoyitol treatment both in vivo and in vitro. These results suggested that sequoyitol ameliorates the progression of diabetic nephropathy in rats, as induced by a high-fat diet and a low dose of streptozotocin, through its glucose-lowering effects, antioxidant activity, and regulation of TGF-β1 expression.

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