Sequoyitol ameliorates diabetic nephropathy in diabetic rats induced with a high-fat diet and a low dose of streptozotocin

2014 ◽  
Vol 92 (5) ◽  
pp. 405-417 ◽  
Author(s):  
Xian-Wei Li ◽  
Yan Liu ◽  
Wei Hao ◽  
Jie-Ren Yang
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Glucose ◽  
High Fat Diet ◽  
Low Dose ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
High Fat

Sequoyitol decreases blood glucose, improves glucose intolerance, and enhances insulin signaling in ob/ob mice. The aim of this study was to investigate the effects of sequoyitol on diabetic nephropathy in rats with type 2 diabetes mellitus and the mechanism of action. Diabetic rats, induced with a high-fat diet and a low dose of streptozotocin, and were administered sequoyitol (12.5, 25.0, and 50.0 mg·(kg body mass)−1·d−1) for 6 weeks. The levels of fasting blood glucose (FBG), serum insulin, blood urea nitrogen (BUN), and serum creatinine (SCr) were measured. The expression levels of p22phox, p47phox, NF-κB, and TGF-β1 were measured using immunohistochemisty, real-time PCR, and (or) Western blot. The total antioxidative capacity (T-AOC), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were also determined. The results showed that sequoyitol significantly decreased FBG, BUN, and SCr levels, and increased the insulin levels in diabetic rats. The level of T-AOC was significantly increased, while ROS and MDA levels and the expression of p22phox, p47phox, NF-κB, and TGF-β1 were decreased with sequoyitol treatment both in vivo and in vitro. These results suggested that sequoyitol ameliorates the progression of diabetic nephropathy in rats, as induced by a high-fat diet and a low dose of streptozotocin, through its glucose-lowering effects, antioxidant activity, and regulation of TGF-β1 expression.

scholarly journals Curcumin improves glycolipid metabolism through regulating peroxisome proliferator activated receptor γ signalling pathway in high-fat diet-induced obese mice and 3T3-L1 adipocytes

2017 ◽  
Vol 4 (11) ◽  
pp. 170917 ◽  
Author(s):  
Yanyun Pan ◽  
Dandan Zhao ◽  
Na Yu ◽  
Tian An ◽  
Jianan Miao ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Fasting Blood Glucose ◽  
Signalling Pathway ◽  
Peroxisome Proliferator ◽  
Obese Mice ◽  
High Fat ◽  
Peroxisome Proliferator Activated Receptor ◽  
Glycolipid Metabolism

Curcumin is an active component derived from Curcuma longa L. which is a traditional Chinese medicine that is widely used for treating metabolic diseases through regulating different molecular pathways. Here, in this study, we aimed to comprehensively investigate the effects of curcumin on glycolipid metabolism in vivo and in vitro and then determine the underlying mechanism. Male C57BL/6 J obese mice and 3T3-L1 adipocytes were used for in vivo and in vitro study, respectively. Our results demonstrated that treatment with curcumin for eight weeks decreased body weight, fat mass and serum lipid profiles. Meanwhile, it lowered fasting blood glucose and increased the insulin sensitivity in high-fat diet-induced obese mice. In addition, curcumin stimulated lipolysis and improved glycolipid metabolism through upregulating the expressions of adipose triglyceride lipase and hormone-sensitive lipase, peroxisome proliferator activated receptor γ/α (PPARγ/α) and CCAAT/enhancer binding proteinα (C/EBPα) in adipose tissue of the mice. In differentiated 3T3-L1 cells, curcumin reduced glycerol release and increased glucose uptake via upregulating PPARγ and C/EBPα. We concluded that curcumin has the potential to improve glycolipid metabolism disorders caused by obesity through regulating PPARγ signalling pathway.

scholarly journals In vitro, acute and subchronic evaluation of the antidiabetic activity of Atractylis flava Desf n-butanol extract in alloxan-diabetic rats

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mohamed Akram Melakhessou ◽  
Salah Eddine Marref ◽  
Naima Benkiki ◽  
Cherine Marref ◽  
Imene Becheker ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Plant Extract ◽  
Fasting Blood Glucose ◽  
Folk Medicine ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Oral Glucose ◽  
Butanol Extract

Abstract Background Diabetes mellitus is a serious complex multifactorial disorder that imposes huge health and economic burden on societies. Because the currently available medications have many drawbacks, it's important to look for alternative therapies. Medicinal plants utilized in folk medicine are ideal candidates. Therefore, this work assessed the antidiabetic action of n-butanol extract from the whole plant Atractylis flava Desf (BEAF). These ethnomedicinal properties of BEAF were scientifically validated using in vitro and in vivo assays. In vitro antidiabetic effect of the BEAF was conducted using α-Glucosidase and α-Amylase assays. While the antihyperglycemic activity was assessed using two rat models: Alloxan-induced diabetic rats and oral glucose challenged rats. Experimental diabetes was induced by a single intraperitoneal injection of alloxan at a dose of 150 mg/kg and animals with fasting blood glucose levels (BGL) > 200 mg/dL were considered diabetic. Glibenclamide (5 mg/kg) was used as a typical drug. Results The BEAF at all tested dose levels (100, 250, and 500 mg/kg) showed a significant decrease in blood glucose level in all the two animal models. Besides, the plant extract exhibited a potent inhibitory effect on α-Amylase and α-Glucosidase activity at a concentration of 1000 µg/mL with 76.17% and 89.37%, respectively. Conclusion BEAF exerts in vitro and in vivo antidiabetic effects, these results suggest that the plant extract can be a therapeutic resource in the treatment of diabetes and hyperlipidemia.

scholarly journals Sanziguben polysaccharides inhibit diabetic nephropathy through NF-κB-mediated anti-inflammation

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Kang Zhou ◽  
Jianing Zhang ◽  
Chang Liu ◽  
Lijuan Ou ◽  
Fan Wang ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
High Fat Diet ◽  
In Vitro Model ◽  
Critical Role ◽  
High Fat ◽  
Chinese Medicines ◽  
Vitro Model ◽  
Anti Inflammation

Abstract Background Sanziguben polysaccharides (SZP) are large amounts of classical Chinese medicines from Sanziguben (SZGB). Moreover, SZGB is a widely applied compound prescription for diabetic nephropathy (DN) treatment, but the role is still unclear. This study initially explores the mechanism of SZP in the treatment of DN. Methods The high-fat diet plus streptozotocin injections were used to replicate the DN models in male C57BL/6 mice. DN mice were divided into five groups: DN mice, DN mice treated with SZP(1.01 or 2.02 g/kg), DN mice treated with SZGB decoction(4.7 g/kg), and DN mice treated with metformin (300 mg/kg). HG and LPS plus TNFα stimulated human tubule epithelial (HK-2) cells to establish an in vitro model and treated with SZP (100 or 200 μg/mL). Results SZP was found to comprise sugar, protein, and uronic acid. Furthermore, SZP alleviated the progression of inflammation in vivo and in vitro by inhibiting the expression of NF-κB. Conclusions NF-κB plays a critical role in the development of DN induced by STZ and HG. Furthermore, SZP can attenuate the NF-κB‐mediated progression of diabetic nephropathy, improve DN through anti-inflammation.

scholarly journals A COMPARATIVE STUDY OF THE ANTI-OXIDATIVE AND ANTI-DIABETIC POTENTIAL OF IN VITRO AND IN VIVO ROOT AND LEAF EXTRACTS OF WITHANIA SOMNIFERA ON STREPTOZOTOCIN INDUCED DIABETIC RATS

2016 ◽  
Vol 8 (10) ◽  
pp. 85 ◽  
Author(s):  
Somanatha Jena ◽  
Ram C. Jena ◽  
Rasmita Bhol ◽  
Khusbu Agarwal ◽  
Ansuman Sarangi ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Withania Somnifera ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Root Extract ◽  
Leaf Extracts ◽  
Root Extracts

<p><strong>Objective: </strong>The present investigation explores the possibilities of using the <em>in vitro</em> and <em>in vivo </em>root and leaf extracts of <em>Withania somnifera </em>for anti-diabetic and anti-hyperlipidaemic effects on streptozotocin-induced diabetic rats.</p><p><strong>Methods: </strong><em>In vitro </em>shoot cultures of <em>Withania somnifera</em> were raised by the axillary proliferation in nodal explants from a garden grown plant using Murashige and Skoog medium then <em>in</em><em> vitro</em> raised roots and shoots were used for the anti-hyperglycemic and anti-hyperlipidaemic experiment. After 72 h of STZ administration, the fasting blood glucose levels were measured and the rats showing FBG level&gt;220 mg/dl were considered to be diabetic and were used for the hyperglycemic study. <em>In vitro</em> and <em>in vivo</em> methanolic root and leaf extracts were orally administered daily to diabetic rats for eight weeks. After the treatment period, blood glucose and serum enzymes like aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total cholesterol, triglycerides, HDL-c high density lipoprotein-bound cholesterol, LDL-c low density lipoprotein-bound cholesterol, LDH, serum protein level, total phenolics and anti-oxidative analysis (DPPH and FRAP) were determined.</p><p><strong>Results: </strong>The levels of blood glucose, AST, ALT, ALP, LDH, HDL-c significantly increased by the use of <em>in vitro</em> methanolic root extracts compared to normal control rats. However, remarkable loss of total protein, albumin, albumin: globulin (A: G) ratio was reported in streptozotocin-induced diabetic rats by using <em>in vitro</em> root extracts. Methanolic <em>in vitro</em> root extract at the dose levels of 300 mg/kg body weight produced a significant decrease in fasting blood glucose (FBG) level by 102.65 with respect to initial fasting blood glucose level after 30 d of the treatment. <em>In vitro</em> root extract demonstrated highest DPPH and FRAP free radical scavenging activity, i.e. 86.55±1.77 and 48.87±2.55 than other extracts.</p><p><strong>Conclusion: </strong>It may be concluded that methanolic <em>in vitro</em> root extract <em>W. somnifera </em>at the dose (300 mg/kg) has more potent anti-hyperglycaemic activity than the other <em>in vitro</em> and <em>in vivo </em>extracts of leaf and root on streptozotocin induced diabetic rats and was also found to be similar in effect to that of the standard drug ‘Glibenclamide’.</p>

scholarly journals Antidiabetic Effect of Fresh Nopal (Opuntia ficus-indica) in Low-Dose Streptozotocin-Induced Diabetic Rats Fed a High-Fat Diet

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Seung Hwan Hwang ◽  
Il-Jun Kang ◽  
Soon Sung Lim
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
High Fat Diet ◽  
Low Dose ◽  
Water Extract ◽  
Diabetic Rats ◽  
Control Group ◽  
High Fat ◽  
Glucose Levels ◽  
Blood Glucose Levels

The objective of the present study was to evaluateα-glucosidase inhibitory and antidiabetic effects of Nopal water extract (NPWE) and Nopal dry power (NADP) in low-dose streptozotocin- (STZ-) induced diabetic rats fed a high-fat diet (HFD). The type 2 diabetic rat model was induced by HFD and low-dose STZ. The rats were divided into four groups as follows: (1) nondiabetic rats fed a regular diet (RD-Control); (2) low-dose STZ-induced diabetic rats fed HFD (HF-STZ-Control); (3) low-dose STZ-induced diabetic rats fed HFD and supplemented with NPWE (100 mg/kg body weight, HF-STZ-NPWE); and (4) low-dose STZ-induced diabetic rats fed HFD and supplemented with comparison medication (rosiglitazone, 10 mg/kg, body weight, HF-STZ-Rosiglitazone). In results, NPWE and NADP had IC50values of 67.33 and 86.68 μg/mL, both of which exhibit inhibitory activities but lower than that of acarbose (38.05 μg/mL) while NPWE group significantly decreased blood glucose levels compared to control and NPDP group on glucose tolerance in the high-fat diet fed rats model (P<0.05). Also, the blood glucose levels of HR-STZ-NPWE group were significantly lower (P<0.05) than HR-STZ-Control group on low-dose STZ-induced diabetic rats fed HFD. Based on these findings, we suggested that NPWE could be considered for the prevention and/or treatment of blood glucose and a potential use as a dietary supplement.

scholarly journals ANTIDIABETIC POTENTIAL OF PERILLYL ALCOHOL, A MONOTERPENE ON HIGH-FAT DIET LOW-DOSE STREPTOZOTOCIN-INDUCED DIABETES IN EXPERIMENTAL RATS

2019 ◽  
pp. 230-235
Author(s):  
TOWSEEF HASSAN ◽  
ELANCHEZHIYAN C ◽  
INSHA NASEER
Keyword(s):  
Blood Glucose ◽  
High Fat Diet ◽  
Low Dose ◽  
Histological Study ◽  
Perillyl Alcohol ◽  
Diabetic Rats ◽  
Control Group ◽  
High Fat ◽  
Plasma Parameters ◽  
Reference Drug

Objective: The aim of the study was to evaluate the antidiabetic potential of Perillyl alcohol (POH) in high-fat diet (HFD) and low-dose streptozotocin (STZ)-induced diabetic Wistar rats. Methods: In experimental rats fed with HFD for 4 weeks, diabetes was induced by single intraperitoneal injection of STZ (35 mg/kg body weight [BW]). Three days after STZ induction, the hyperglycemic rats were treated with POH orally at the doses of 50 and 100 mg/kg BW daily for 30 days. Glibenclamide (6 mg/kg BW) was used as reference drug. Blood glucose level, BW, fluid intake, and food intake such as parameters were analyzed throughout the experiment period. Serum and plasma biochemical parameters were also estimated. Assay on insulin resistance was also done. Histological study of the pancreas and liver was also performed. Results: POH orally at the doses of 50 and 100 mg/kg BW for 30 days significantly (p<0.05) and dose-dependently reduced and normalized blood glucose levels as compared to that of HFD+STZ control group. Serum and plasma parameters were significantly (p<0.05) restored toward the normal levels in POH-treated rats as compared to HFD-STZ control animals. Histological study of liver and pancreas also shows the prominent regeneration in POH and glibenclamide treated rats. Conclusion: The present study concludes that POH demonstrated promising antidiabetic action in HFD STZ-induced diabetic rats.

scholarly journals ALOE-EMODIN GLYCOSIDES AMELIORATE GLUCOSE UTILIZATION VIA INSULIN DOWNSTREAM REGULATORS: AN IN VIVO INVESTIGATION

2016 ◽  
pp. 191 ◽  
Author(s):  
Singaravelu Anand ◽  
Saravanababu C ◽  
Lakshmi Bs ◽  
Muthusamy Vs
Keyword(s):  
Type 2 Diabetes ◽  
Insulin Receptor ◽  
Carbohydrate Metabolism ◽  
High Fat Diet ◽  
Low Dose ◽  
Diabetic Rats ◽  
High Fat ◽  
Aloe Emodin

<p>ABSTRACT<br />Objective: Aloe-emodin glycosides (AEG) isolated from Cassia fistula stimulates glucose transport and glycogen storage through a phosphatidylinositol<br />3 kinase (PI3K)-dependent mechanism in L6 myotubes and inhibits adipocytes differentiation in 3T3L1 adipocytes was previously reported. This<br />study intended to investigate the insulin mimetic effect of AEG by in vivo method.<br />Methods: Male Wistar albino rats were randomly allocated into two groups and fed for a period of 3-week. The high-fat diet group animals were<br />injected with a low dose (35 mg/kg) of streptozotocin to induce Type-2 diabetes. The diabetic rats were then treated with low dose: 10 mg/kg and<br />high dose: 30 mg/kg for a period of 21-day. A dose-dependent decrease in fasting blood glucose, cholesterol, and triglycerides levels on treatment<br />with AEG. The carbohydrate metabolism in diabetic rats appeared to improve due to regulation in hepatic enzymes such as hexokinase, glucose-6phosphatase,<br />and fructose<br />1,6-bisphosphatase with a concomitant increase<br />in glycogen<br />content.<br />Results: AEG decreased lipid peroxidation and improved the antioxidant (enzymatic and nonenzymatic) levels in the liver of diabetic rats. Treatment<br />with AEG (30 mg/kg) augmented the phosphorylation of insulin downstream regulators such as insulin receptor beta, insulin receptor substrate 1,<br />PI3K, glucose transporter 4, glycogen synthase kinase 3 beta, and peroxisome proliferator activator receptor gamma in the skeletal muscle tissue of<br />the Type-2 diabetic rats compared to vehicle-treated diabetic rats.<br />Conclusion: The present results suggested that AEG could serve as an interesting candidate in the drug development for the management of diabetes.<br />Keywords: Aloe-emodin glycoside, Type-2 diabetes, High-fat diet/streptozotocin, Carbohydrate Metabolism, Glycogen, Antioxidant enzyme.</p>

scholarly journals PROTECTIVE ROLE OF BERBERINE IN AMELIORATING DIABETIC COMPLICATIONS IN STREPTOZOTOCIN-HIGH FAT DIET MODEL IN EXPERIMENTAL ANIMALS

2020 ◽  
pp. 41-48
Author(s):  
DEEPTI D. BANDAWANE ◽  
SHRUTI B. MOOLIYA ◽  
SHAILAJA B. JADHAV
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Urea Nitrogen ◽  
Diabetic Complications ◽  
High Fat Diet ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Protective Role ◽  
Left Ventricular ◽  
High Fat ◽  
Urea Nitrogen

Objective: Diabetes mellitus is a serious, complex metabolic disorder and growing health threat disease in the world. Berberine, one of the main constituent in Rhizoma coptidis is widely used in the treatment of diabetes. Potential of berberine in the management of diabetic complications, namely diabetic nephropathy and cardiomyopathy, is however, not yet explored. The present study was, therefore, undertaken to explore the potential of berberine for the management of diabetic nephropathy and diabetic cardiomyopathy in high-fat diet (HFD) and low dose streptozotocin (STZ) induced diabetes in rats. Methods: Rats were fed a high-fat diet for 4 w followed by a single intraperitoneal dose of streptozotocin (35 mg/kg). Animals were divided in five groups. Berberine was given orally in two different dose levels (75 mg/kg and 150 mg/kg) for 28 d. Metformin (100 mg/kg) was used as a standard antidiabetic drug. At the end of the study, parameters evaluated includes glycemic profile, lipid profile, left ventricular indices, urinary protein, serum creatinine, blood urea nitrogen and cardiac antioxidants. Histopathology of kidney and pancreas was carried out. Results: Berberine treated groups showed a significant decrease in fasting blood glucose, glycosylated Hb, creatinine, blood urea nitrogen and urinary total proteins, whereas there was a significant improvement in serum insulin, liver glycogen, skeletal muscle glycogen and cardiac antioxidant enzymes. Conclusion: Present study indicated that berberine shows a protective role in diabetes-associated renal and cardiovascular complications.

scholarly journals BIOCHEMICAL EVALUATION OF ANTIDIABETIC PROPERTIES OF SWERTIAMARIN, A SECOIRIDOID GLYCOSIDE OF ENICOSTEMMA LITTORALE LEAVES, STUDIED IN HIGH-FAT DIET–FED LOW-DOSE STREPTOZOTOCIN-INDUCED TYPE 2 DIABETIC RATS

2018 ◽  
Vol 11 (10) ◽  
pp. 486 ◽  
Author(s):  
Selvam R ◽  
Muruganantham K ◽  
Subramanian S
Keyword(s):  
High Fat Diet ◽  
Low Dose ◽  
Fasting Blood Glucose ◽  
Glycogen Metabolism ◽  
Diabetic Rats ◽  
High Fat ◽  
Enicostemma Littorale ◽  
Liver And Kidney ◽  
Secoiridoid Glycoside

Objective: Swertiamarin, a secoiridoid glycoside present in the leaves of Enicostemma littorale, is reported to be responsible for its pharmacological and beneficial properties. The present study was aimed to biochemically evaluate the antidiabetic properties of Swertiamarin in high fat diet fed - low dose streptozotocin (STZ)-treated diabetic rats.Methods: High-fat diet-fed low-dose STZ was used to induce experimental type 2 diabetes in rats. Diabetic rats were orally treated with swertiamarin (50 mg/kg b.w./rat/day) for 30 days. The physiological criterions such as food and fluid intake were recorded. Oral glucose tolerance test was performed. The levels of fasting blood glucose, plasma insulin, glycosylated hemoglobin A1c (HbA1c), hemoglobin, and homeostasis model assessment of insulin resistance (HOMA-IR) values were estimated. The activities of key enzymes involved in carbohydrate and glycogen metabolism in the liver and kidney tissues were assayed. The glycogen content in liver tissue was estimated.Result: Oral administration of swertiamarin to diabetic rats established a significant decline in the levels of fasting blood glucose, HbA1c as well as HOMA-IR values and an increase in plasma insulin and hemoglobin levels. The altered activities of key enzymes of carbohydrate and glycogen metabolism in liver and kidney tissues of diabetic rats were restored to near normalcy by swertiamarin treatment.Conclusion: Swertiamarin treatment maintains normoglycemia in diabetic rats by modulating the activities of key carbohydrate and glycogen metabolizing enzymes in the hepatic and renal tissues.

Sign in / Sign up

Export Citation Format

Share Document