Immunoregulation via Adhesion Molecules in Allogenic and Xenogenic Hepatocyte Transplantation to Nagase's Analbuminemic Rats

1997 ◽  
Vol 6 (5) ◽  
pp. 535-536 ◽  
Author(s):  
Hitoshi Horimoto ◽  
Masumi Nozawa ◽  
Norihiro Kokudo ◽  
Masatomo Nakao ◽  
Shigeki Takahashi ◽  
...  
Keyword(s):  
Treated Group ◽  
Hepatocyte Transplantation ◽  
Intercellular Adhesion Molecule ◽  
Control Group ◽  
Lymphocyte Function ◽  
Intercellular Adhesion Molecule 1 ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

We investigated the effects of monoclonal antibodies (mAbs) against lymphocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) on intrasplenic allogenic and xenogenic hepatocyte transplantation (HCTx) to analbuminemic rats. Ten to 12-wk-old male Nagase's analbuminemic rats (RT1l) were used as recipients, Wistar/Shi rats (RTlk) were used as donors for allografts and BALB/C mice were used as donors for xenografts. The experimental groups were as follows: group 1, allo-HCTx (n = 7); group 2, allo-HCTx + antirat ICAM-1/antirat LFA-1 mAbs (1.0 mg/kg/day, for 7 days, respectively) (n = 6); group 3, xeno-HCTx (n = 5); group 4, xeno-HCTx + mAbs (antimouse LFA-1/antirat ICAM-1) (n = 5). group 5, xeno-HCTx + mAbs (antirat LFA-1/antimouse ICAM-1) (n = 5). Serum rat albumin levels were measured in groups 1 and 2, and serum mouse albumin levels were measured in groups 3, 4, and 5, as indicators of the function of grafted hepatocytes. In allotransplantation groups, the serum rat albumin levels in the mAbs-treated group were significantly higher than those in the control group for 6 wk after transplantation. In xenotransplantation groups, no increase in the serum mouse albumin levels was detected in any group.

scholarly journals Ameilorative effect of lycopine against cisplatin toxicity in rats

2021 ◽  
Vol 9 (2) ◽  
pp. 84
Author(s):  
Fatma Ehgendy ◽  
Rania M. Waheed ◽  
Samer Ibrahim ◽  
Elshaimaa Said ◽  
Faten Elsayed
Keyword(s):  
Corn Oil ◽  
Protective Efficacy ◽  
Treated Group ◽  
Control Group ◽  
Once Daily ◽  
Group 4 ◽  
Total Antioxidant ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

The following study aimed to investigate the hepato and neuro protective efficacy of Lycopine against Cisplatin which induced hepatotoxicity and neurotoxicity. Twenty Five male Wister rats were used for this experiment they were equally divided into 5 groups (5 rats per group): group (1) served as control group they were injected 1ml saline orally once daily for 20 day, group (2) served as Corn Oil group and they were administrated 1 mL Corn Oil orally once daily for 20 days, group (3) served as Lycopine group and they were administrated (10 mg/kg b.wt) Lycopine orally once daily for 20 days. , group (4) served as Cisplatin treated group and they were injected (6 mg/kg b.wt.) intrapertonialy once at day 10 of experiment and group (5) Lycopine+Cisplatin group and were administrated 10 mg/kg b.wt Lycopine orally once daily for 20 days and injected 6 mg/kg b.wt.) intraperitonialy once at day 10 . Result revealed that Cisplatin induced liver damage indicated by significant increase in liver biomarkers ALP, AlT, AST along with significant decrease in albumin, Moreover marked increase increase in tissue concentrations of malondialdehyde(MDA) and Total antioxidant(TAC) and reduce tissue Glutathione reductase(GSH),that indicated oxidative stress Also results revealed up regulation IL-6 and down regulation IL-10 in liver and brain tissue in compared to control group . However, interestingly concurrent adminsteration of the Lycopine orally at dose level of 10mg/kg b.wt for 20 days with Cisplatin can mitigate these toxic effects caused by Cisplatin.So it is concluded that the antioxidant and the anti-inflammatory effects of Lycopine moderate the Cisplatin-induced hepato and neurotoxicity.

scholarly journals Effectiveness of medical treatment in overcoming the ureteral double-J stent related symptoms

2012 ◽  
Vol 6 (6) ◽  
pp. 234 ◽  
Author(s):  
Ugur Kuyumcuoglu ◽  
Bilal Eryildirim ◽  
Murat Tuncer ◽  
Gokhan Faydaci ◽  
Fatih Tarhan ◽  
...  
Keyword(s):  
The Body ◽  
Control Group ◽  
Group 4 ◽  
Double J Stent ◽  
Urinary Tract Symptoms ◽  
Stone Treatment ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

Background: We investigated whether the frequency of lower urinary tract symptoms (LUTS) increased in patients in whom double-J stents were applied. We also evaluated several medical therapy protocols to treat symptoms related with ureteral stents.Materials and Methods: A total of 108 patients, in whom unilateral double-j stent was applied during ureteral stone treatment, were included. Before the double-J stent was applied, all patients completed storage components of the “International Prostate Symptom Score” (IPSSs), quality of life components of the IPSS (IPSS-QOL) and “Overactive Bladder Questionnaire” (OABq) forms and scores were calculated. After the procedure, cases were randomized into 5 groups, an antiinflammatory was given to Group 1, spasmolytic to Group 2, anticholinergic to Group 3 and α-blocker to Group 4. No additional drug was given to Group 5 as this control group. During the fourth week of the procedure, IPSSs, IPSS-QOL and OABq forms were again completed and scores were compared with the previous ones.Results: When all the cases were evaluated, the IPSSs, IPSS-QOLand OABq scores of patients in whom the double-J stent was applied were statistically significantly higher the procedure. Compared to the control group, the cases where the double-J stent was applied showed a higher IPSSs, IPSS-QOL and OABq scores and none of the medical therapies could prevent this increase.Interpretation: The frequency of LUTS increased in cases where the ureteral stent was applied and discomfort continued as long as the stent stayed in the body.

scholarly journals Zingerone Ameliorates Hepatotoxicity

2020 ◽  
Vol 11 (1) ◽  
pp. 280-284 ◽  
Author(s):  
Julietpoornamathy J ◽  
Parameswari C.S.
Keyword(s):  
The Body ◽  
Control Group ◽  
Medical Sciences ◽  
Toxic Compounds ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Insight Into ◽  
Group 1

In medical sciences, toxicity is an area wherein extensive studies have been carried to improve the diseases as well as to prevent. So, there is a high requirement for novel and improved alternative therapeutic strategies to manage diseases. The liver is the largest gland in the body, which executes several important mechanisms; it stores minerals and vitamins and releases them in periods of need. The main aim of this study was to give a closer insight into potent non- toxic compounds that is capable of modifying the responses. Animals were divided into five equal groups viz control (Group 1), administered with food and water ad libitum, (Group 2) administered with olive oil, (Group 3) administered with zingerone, (Group 4) administered with concanavalin A, (Group 5) administered with cyclosporine A followed by zingerone. Our results revealed significant changes in liver marker enzymes and liver histology of zingerone treated rats when compared to control rats.  A corollary, zingerone has no toxic effect on hepatocytes and was found to be safe at a dose of 10mg/kg b wt and also ameliorates hepatotoxicity.

scholarly journals FE-SEM Evaluation of Dental Specimens Prepared by Different Methods forIn VitroContamination

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Vitor Cesar Nakamura ◽  
Simony Hidee Hamoy Kataoka ◽  
Giulio Gavini ◽  
Patrícia Helena Ferrari ◽  
Silvana Cai
Keyword(s):  
Control Group ◽  
Buffer Solution ◽  
Morphological Alterations ◽  
Ultrasonic Bath ◽  
Group 4 ◽  
Cement Removal ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Objective. To evaluate through FE-SEM the cleanliness and dentinal alterations promoted by different methods of dental sample preparation.Methods. Twenty-five human single-rooted teeth were used. The teeth were cleaned and autoclaved in wet medium and randomly divided into 5 groups (), according to the preparation methods employed—control group: no solutions applied; group 1: cement removal and irrigation with 5.25% % EDTA for 4 minutes each; group 2: 17%  % NaOCl (4 minutes ultrasonic bath); group 3: cement removal and 17%     buffer solution + distilled water (10 minutes ultrasonic); group 4: 17%   NaOCl (3 minutes ultrasonic bath). Specimens were analyzed by field emission scanning electron microscope (FE-SEM), at 1500x magnification. Data were submitted to qualitative analysis according to a scoring system and submitted to Kruskal-Wallis test.Results. In ascending order, as to bind parameters, (i) cleanliness: control, group 2, group 3, group 5, and group 4, (ii) dentinal alterations: group 1, group 5, group 2, group 3, and group 4.Conclusion. The proposed protocol was suitable for subsequent microbiological contamination, because it showed less dentinal morphological alterations with increased removal of organic waste.

scholarly journals Cerebral Hemodynamics and Cognitive Function in Cirrhotic Patients with Hepatic Encephalopathy

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Yuqing Zhou ◽  
Qian Dong ◽  
Rong Zhang ◽  
Shunfeng Zhou ◽  
Linqiang Li ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Treated Group ◽  
Cerebral Hemodynamics ◽  
Control Group ◽  
Group 4 ◽  
Group A ◽  
Cirrhotic Patients ◽  
Group 3 ◽  
Group 2 ◽  
Group B

Aims.To investigate cerebral hemodynamics in cirrhotic patients with HE and to observe effects of treatment in cerebral hemodynamics and correlations among ammonia, cerebral hemodynamics, and cognitive function.Methods. There were four groups: healthy controls (group 1), cirrhosis without HE (group 2), cirrhosis with MHE (group 3), and cirrhosis with OHE (group 4). Ammonia and cerebral hemodynamics (by TCD) were assessed. Patients in group 3 were subsequently randomized to two subgroups: the control (group A) and the treated (group B, treated with lactulose for two months), and they were retested for ammonia and TCD after treatment. Results. Ammonia,Vm,Vd, PI, and RI were statistically different before treatment, and ammonia, PI, and RI levels paralleled the severity of HE (P<0.05). In group B,Vdincreased and ammonia, PI, and RI declined following treatment (P<0.05), while there were no differences in group A (P>0.05). Correlations were found between ammonia andVd, PI, RI, NCT-A, and DST and also found betweenVd, PI, RI, and NCT-A and DST (P<0.05).Conclusions. This study revealed that cerebral hemodynamics were related to the severity of HE and cerebral autoregulation was impaired. There were tight correlations among ammonia, cerebral hemodynamics, and cognitive function, and, following treatment, cerebral hemodynamics improved.

scholarly journals The Evaluation of Various Restoration Techniques on Internal Adaptation of Composites in Class V Cavities

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
D. Dionysopoulos ◽  
C. Papadopoulos ◽  
E. Koliniotou-Koumpia
Keyword(s):  
Room Temperature ◽  
Control Group ◽  
Class V ◽  
Group 4 ◽  
Restoration Techniques ◽  
Group 3 ◽  
Internal Adaptation ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Aim.The aim of this study was to evaluate the effect of different restoration techniques on the formation of internal microgaps between materials and dentin in class V restorations.Materials and Methods.Twenty-five extracted human premolars were prepared with standardized class V cavity outlines (3 mm × 2 mm × 2 mm). The cavities were randomly divided into 5 groups of 10 cavities each and restored according to manufacturer’s instructions: Group 1: preheating (55°C) conventional composite (Filtek Z250), Group 2: flowable composite (Filtek Flow), Group 3: Filtek Flow + Filtek Z250 light-cured separately, Group 4: Filtek Flow + Filtek Z250 light-cured simultaneously, and Group 5 (control): Filtek Z250 at room temperature (23°C). The specimens were then thermocycled and cross-sectioned through the center of the restoration. Subsequently, impressions were taken, and epoxy resin replicas were made. The internal adaptation of the materials to the axial wall was analyzed under SEM.Results.The preheated Filtek Z250 (Group 1) showed better internal adaptation than the room temperature groups(P<0.05). The combination of Filtek Flow with Filtek Z250 which was light-cured separately (Group 3) exhibited better internal adaptation than control group(P<0.05).Conclusion.Different restoration techniques exhibit different behavior regarding internal adaptation to dentin after photopolymerization.

scholarly journals Treatment of ovarian cysts in sows – a field trial

2008 ◽  
Vol 52 (No. 9) ◽  
pp. 413-418 ◽  
Author(s):  
S. Cech ◽  
R. Dolezel
Keyword(s):  
The Other ◽  
Ovarian Cysts ◽  
Control Group ◽  
Initial Examination ◽  
Interval Group ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Different procedures of treatment of large follicular ovarian cysts in 177 sows using GnRH, hCG and PGF2α are evaluated in this study. Ovarian cysts were diagnosed by transcutaneous ultrasonography, which was a part of routine pregnancy diagnosis. No treatment was performed in the control group (Group 1, <i>n</i> = 29); the method of treatment used in the other groups immediately after the diagnosis was intramuscular administration of lecirelin in doses 50 µg (Group 2, <i>n</i> = 28), 100 µg (Group 3, <i>n</i> = 27) and 200 µg divided into 2 equal doses administered at a 12-hour interval (Group 4, <i>n<i> = 25) and of hCG in doses 1 500 IU (Group 5, <i>n</i> = 23), 3 000 IU (Group 6, <i>n</i> = 21), and 250 µg of cloprostenol (Group 7, <i>n</i> = 24). Insemination rate (IR), conception rate (CR) in inseminated sows, pregnancy rate (PR = recovery rate), treatment-insemination interval (TII) and treatment-pregnancy interval (TPI) within 42 days after the initial examination were evaluated. In addition PR in groups of sows divided according to parity (1–3, 4–6 and ≥ 7) were also evaluated. IR and PR were higher in Group 4 (84.0% and 44.0%) and lower in Group 1 (17.2% and 6.9%) in comparison with the other groups (<i>P</i> < 0.001 and <i>P</i> < 0.05). CR, TII and TPI did not differ between the experimental groups. PR were similar in sows with different parity. The study proved a positive response in sows with large follicular ovarian cysts to the treatment consisting of 2 administrations of 100 µg GnRH at a 12-hour interval.

scholarly journals Effects of a probiotic in combination with prebiotics on intestinal lactobacilli and coliforms and activities of bacterial enzymes in 1,2-dimethylhydrazine exposed rats

2011 ◽  
Vol 56 (No. 3) ◽  
pp. 99-106 ◽  
Author(s):  
L. Strojný ◽  
A. Bo ◽  
E. Hijová ◽  
A. Chmelárová ◽  
G. Mojžišová ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Control Group ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Beta Glucosidase ◽  
Alpha Glucosidase ◽  
Beta Galactosidase ◽  
Group 1

Effects of the probiotic (PRO) Lactobacillus plantarum and of the combination of PRO and the prebiotic (PRE) inulin enriched with oligofructose (2%), and PRO with Lini oleum virginale (O) on counts of lactobacilli and coliforms and enzymatic activities in faeces of rats were studied. The rats (n = 60) were divided into 5 groups of 12 subjects. The animals were fed on a high fat diet (10%) for 8 weeks of experiment. Colon cancer was induced by the application of 1,2-dimethylhydrazine (DMH) twice a week in a dose of 20 mg/kg s.c. in groups G2-G5. The rats in group 1 (control 1) received a diet without any supplements. The rats in group 2 (control 2) received 1,2 DMH without any supplements. The rats in group 3 received PRO, group 4 PRO and PRE, and group 5 received PRO and O. A significant decrease (P &lt; 0.05) of coliforms was found out after the application of PRO, PRO-O, and PRO-PRE in comparison with control group G2. Significantly higher (P &lt; 0.05) counts of lactobacilli were determined after the application of PRO-O and PRO-PRE. Significantly lower (P &lt; 0.001) activities of &beta;-galactosidase, &beta;-glucuronidase and &alpha;-glucosidase were observed in PRO, PRO-PRE and PRO-O, while in the case of the enzyme &beta;-glucosidase the activity was lower only after the addition of PRO-O. The protective effect of lactobacilli was observed in the order PRO-O, PRO-PRE, and PRO. It was shown that combinations of PRO-O and PRO-PRE had a synergistic effect which was higher than the effect of administering only PRO.

scholarly journals The effect of excess dietary manganese on uninfected and Ascaridia galli infected chicks

1999 ◽  
Vol 73 (4) ◽  
pp. 313-316 ◽  
Author(s):  
M. Gabrashanska ◽  
S. Tepavitcharova ◽  
C. Balarew ◽  
M.M. Galvez-Morros ◽  
P. Arambarri
Keyword(s):  
Worm Burden ◽  
Manganese Content ◽  
Control Group ◽  
Ascaridia Galli ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Different Sources ◽  
Manganese Level

The effect of dietary manganese from two different sources on chicks (uninfected and infected with Ascaridia galli) was studied. Chick diet was supplemented with 0.9 g Mn2+ kg-1 food either in the form of MnSO4.H2O or 2Gly. MnCl2.2H2O for 20 days. Chicks were divided into six groups: group 0, control; group 1, control + MnSO4.H2O; group 2, control + 2Gly.MnCl2.2H2O; group 3, infected with A. galli; group 4, infected with A. galli + MnSO4.H2O; and group 5, infected with A. galli + 2Gly.MnCl2.2H2O. Body weight, mortality, worm burden, and liver manganese content were investigated. Excess dietary manganese increased weights and manganese level, but mortality and worm burden were unaffected. A greater bioavailability of manganese from 2Gly. MnCl2.2H2O was established.

scholarly journals Effect of the number of thermocycles on microleakage of resin composite restorations

2003 ◽  
Vol 17 (4) ◽  
pp. 337-341 ◽  
Author(s):  
Flávia Bittencourt Pazinatto ◽  
Bruno Barbosa Campos ◽  
Leonardo César Costa ◽  
Maria Teresa Atta
Keyword(s):  
Resin Composite ◽  
Control Group ◽  
Group 4 ◽  
Thermal Changes ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Number Of Cycles ◽  
Group 1

Thermocycling simulates, in vitro, thermal changes that occur in the oral cavity. The aim of this study was to evaluate the influence of the number of cycles on microleakage. Class V cavities (1.5 mm deep, 3 mm in height and 3 mm in width) were prepared in bovine teeth, restored with a Single Bond/Z250 restorative system (3M/ESPE) and then divided into five groups of ten teeth each: group 1 was not thermocycled (control group), and groups 2, 3, 4 and 5 were thermocycled 500, 1,000, 2,500 and 5,000 times, respectively (5º-55º ± 2ºC, 15 s dwell time). The teeth were immersed in 0.5% basic fuchsin aqueous solution for 24 h, sectioned and the sections with the highest degree of microleakage were selected, scanned and the extent of dye penetration was measured by the ImageTool program. The results submitted to one-way ANOVA showed no significant differences between the groups (p > 0.05). The averages of microleakage values in millimeters were: group 1 (3.92); group 2 (3.13); group 3 (4.48); group 4 (4.33) and group 5 (3.42). Thus, it was concluded that there is no relation between the increase of the number of cycles and the increase in microleakage.

Sign in / Sign up

Export Citation Format

Share Document