scholarly journals Development and Validation of Monoclonal and Polyclonal Antibodies for the Detection of Immunoglobulin G of Bottlenose Dolphins (Tursiops Truncatus)

2007 ◽  
Vol 19 (5) ◽  
pp. 465-470 ◽  
Author(s):  
Hendrik H. Nollens ◽  
Linda G. Green ◽  
Diane Duke ◽  
Michael T. Walsh ◽  
Beth Chittick ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Polyclonal Antibody ◽  
Immunoglobulin G ◽  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Bottlenose Dolphins ◽  
Enzyme Linked Immunosorbent Assay ◽  
Common Bottlenose Dolphin ◽  
Circulating Antibodies ◽  
The Common

Antibodies directed against species-specific immunoglobulin G (IgG) have a broad range of applications in serologic and immunologic research and in the development of clinical assays. Validated anti-IgG antibodies for marine mammal species are in short supply. The objective of this study was to produce and validate antibodies with specificity for IgG of the common bottlenose dolphin ( Tursiops truncatus). Bottlenose dolphin IgG was purified using protein G. Two mouse monoclonal antibodies and a rabbit polyclonal antibody were developed from mice and rabbits immunized with bottlenose dolphin IgG. The specificity of the monoclonal antibodies and the polyclonal antibody for bottlenose dolphin IgG was first verified by Western blot analysis and enzyme-linked immunosorbent assay (ELISA). For further validation, both monoclonal antibodies and the polyclonal antibody were incorporated in an indirect ELISA for the detection of the immune response of bottlenose dolphins to a vaccine antigen. Three bottlenose dolphins were immunized with a commercial Erysipelothrix rhusiopathiae vaccine, and serial blood samples were collected from all dolphins for measurement of levels of circulating antibodies. Seroconversion was observed in all 3 dolphins by use of both monoclonal antibodies and the polyclonal antibody. Circulating antibodies were detectable as early as 6 days after immunization in 1 dolphin. Peak antibody levels were detected 14 days after the immunization. The ability to detect seroconversion in all 3 immunized bottlenose dolphins firmly establishes the specificity of the monoclonal antibodies and the polyclonal antibody for IgG of the common bottlenose dolphin.

scholarly journals Genetic divergence and fine scale population structure of the common bottlenose dolphin (Tursiops truncatus, Montagu) found in the Gulf of Guayaquil, Ecuador

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4589 ◽  
Author(s):  
Rosa de los Ángeles Bayas-Rea ◽  
Fernando Félix ◽  
Rommel Montufar
Keyword(s):  
Population Structure ◽  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Bottlenose Dolphins ◽  
Western Coast ◽  
Common Bottlenose Dolphin ◽  
Dolphin Population ◽  
The Common ◽  
Gulf Of Guayaquil ◽  
Outer Estuary

The common bottlenose dolphin, Tursiops truncatus, is widely distributed along the western coast of South America. In Ecuador, a resident population of bottlenose dolphins inhabits the inner estuarine area of the Gulf of Guayaquil located in the southwestern part of the country and is under threat from different human activities in the area. Only one genetic study on South American common bottlenose dolphins has been carried out to date, and understanding genetic variation of wildlife populations, especially species that are identified as threatened, is crucial for defining conservation units and developing appropriate conservation strategies. In order to evaluate the evolutionary link of this population, we assessed the phylogenetic relationships, phylogeographic patterns, and population structure using mitochondrial DNA (mtDNA). The sampling comprised: (i) 31 skin samples collected from free-ranging dolphins at three locations in the Gulf of Guayaquil inner estuary, (ii) 38 samples from stranded dolphins available at the collection of the “Museo de Ballenas de Salinas,” (iii) 549 mtDNA control region (mtDNA CR) sequences from GenBank, and (iv) 66 concatenated sequences from 7-mtDNA regions (12S rRNA, 16S rRNA, NADH dehydrogenase subunit I–II, cytochrome oxidase I and II, cytochrome b, and CR) obtained from mitogenomes available in GenBank. Our analyses indicated population structure between both inner and outer estuary dolphin populations as well as with distinct populations of T. truncatus using mtDNA CR. Moreover, the inner estuary bottlenose dolphin (estuarine bottlenose dolphin) population exhibited lower levels of genetic diversity than the outer estuary dolphin population according to the mtDNA CR. Finally, the estuarine bottlenose dolphin population was genetically distinct from other T. truncatus populations based on mtDNA CR and 7-mtDNA regions. From these results, we suggest that the estuarine bottlenose dolphin population should be considered a distinct lineage. This dolphin population faces a variety of anthropogenic threats in this area; thus, we highlight its fragility and urge authorities to issue prompt management and conservation measures.

Taxonomy and distribution of bottlenose dolphins (genus Tursiops) in Australian waters: an osteological clarification

2020 ◽  
Vol 98 (7) ◽  
pp. 461-479 ◽  
Author(s):  
M. Jedensjö ◽  
C.M. Kemper ◽  
M. Milella ◽  
E.P. Willems ◽  
M. Krützen
Keyword(s):  
Bottlenose Dolphin ◽  
Bottlenose Dolphins ◽  
Three Dimensional ◽  
Large Sample Size ◽  
Type Specimens ◽  
Tursiops Aduncus ◽  
Common Bottlenose Dolphin ◽  
Intermediate Size ◽  
The Common ◽  
Taxonomic Studies

Species relationships in the bottlenose dolphin (genus Tursiops Gervais, 1855) are controversial. We carried out a comprehensive osteological study of 264 skulls, including type specimens, and 90 postcranial skeletons of Tursiops spp. to address taxonomic uncertainties in Australia using two-dimensional (2D) measurements, and three-dimensional geometric morphometrics (3DGM), tooth and vertebral counts, and categorical data. Analyses provided support for the presence of two forms, aligned to the Indo-Pacific bottlenose dolphin (Tursiops aduncus (Ehrenberg, 1832)) and the common bottlenose dolphin (Tursiops truncatus (Montagu, 1821)), including type specimens. The Burrunan dolphin (Tursiops australis Charlton-Robb, Gershwin, Thompson, Austin, Owen and McKechnie, 2011) fell well within T. truncatus for both 2D and 3DGM methods. Thirteen Tursiops spp. specimens, no T. australis specimens, were of intermediate size (2D) and could not be assigned to either species. For 3DGM data, there was a strong allometric influence and few non-allometric differences between species. Length and width of the cranium and rostrum were important discriminating variables. Tursiops aduncus was smaller, had more teeth, fewer vertebrae, and more erosion on the pterygoids and frontals than T. truncatus. Overall cranium shape was round in T. aduncus and angular in T. truncatus. Skull length of T. aduncus was smaller in low than in high latitudes. This study highlights the importance of large sample size, multiple analytical methods, and extensive geographical coverage when undertaking taxonomic studies.

scholarly journals Study on the Ejaculate Characteristics and Liquid Storage of Semen in the Common Bottlenose Dolphin (Tursiops truncatus)

2013 ◽  
Vol 18 (3) ◽  
pp. 107-114
Author(s):  
Asami TAKENAKA ◽  
Nobuyuki KASHIWAGI ◽  
Yuko MAEZONO ◽  
Tatsuko NAKAO ◽  
Yuka WANO ◽  
...  
Keyword(s):  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Liquid Storage ◽  
Common Bottlenose Dolphin ◽  
The Common

Behaviorally measured tactile sensitivity in the common bottlenose dolphin, Tursiops truncatus

2020 ◽  
Vol 36 (3) ◽  
pp. 802-812
Author(s):  
Madelyn G. Strahan ◽  
Dorian S. Houser ◽  
James J. Finneran ◽  
Jason Mulsow ◽  
Daniel E. Crocker
Keyword(s):  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Tactile Sensitivity ◽  
Common Bottlenose Dolphin ◽  
The Common

scholarly journals Labeled quantitative mass spectrometry to study the host response during aspergillosis in the common bottlenose dolphin (Tursiops truncatus)

2019 ◽  
Vol 232 ◽  
pp. 42-49
Author(s):  
Guillaume Desoubeaux ◽  
Maria del Carmen Piqueras ◽  
Carolina Le-Bert ◽  
Vanessa Fravel ◽  
Tonya Clauss ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Host Response ◽  
Quantitative Mass Spectrometry ◽  
Common Bottlenose Dolphin ◽  
The Common

scholarly journals Aspectos etnoecológicos e implicações para a conservação de Tursiops truncatus (Cetartiodactyla: Delphinidae) no Arquipélago das Cagarras, Rio de Janeiro, Brasil

2013 ◽  
Vol 13 ◽  
Author(s):  
Liliane Lodi ◽  
Camilah Antunes Zappes ◽  
Álvaro Samuel Guimarães dos Santos
Keyword(s):  
Tursiops Truncatus ◽  
Rio De Janeiro ◽  
Anthropogenic Impacts ◽  
Participant Observation ◽  
Bottlenose Dolphins ◽  
Morphological Characteristics ◽  
Small Scale ◽  
Common Bottlenose Dolphin ◽  
Significant Difference ◽  
The Common

The objectives of this study were to understand the ecological aspects of the common bottlenose dolphin (Tursiops truncatus) in the Cagarras Archipelago, Rio de Janeiro, Brazil, and possible anthropogenic impacts by comparing the knowledge of local small-scale fishermen (GA) and the regulars in the archipelago (GB). The data obtained were compared with those available in the literature to propose strategies for monitoring and preservation. Between August and December 2010, we applied the methods of participant observation, interviews-questionnaires and use of illustrative posters. Sixty-one interviews were conducted, from which 83.6% were selected by considering correct answers about the identification of the species on posters (morphological characteristics) and description of time of occurrence in the archipelago (July to December) as reported for the species in the literature. Regarding the seven questions analyzed statistically, a significant difference was found between the accounts of the two groups with respect to anthropic threats to T. truncatus. Gill-nets and the decrease of fishing resources were the main causes identified by the GA group whereas the GB group pointed to the disorganized use of space, pollution and predatory fishing as the factors that have threatened the common bottlenose dolphins. The use of ethnobiological data in conservation programs brings opportunities for the collaboration and participation of the community that is directly inserted into the ecosystem as well as a chance for locals to get involved in issues concerning the sustainability of the archipelago.

Machine learning approaches to investigate the impact of PCBs on the transcriptome of the common bottlenose dolphin (Tursiops truncatus)

2014 ◽  
Vol 100 ◽  
pp. 57-67 ◽  
Author(s):  
Annalaura Mancia ◽  
James C. Ryan ◽  
Frances M. Van Dolah ◽  
John R. Kucklick ◽  
Teresa K. Rowles ◽  
...  
Keyword(s):  
Machine Learning ◽  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Learning Approaches ◽  
Common Bottlenose Dolphin ◽  
The Common ◽  
The Impact
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