Isolation and characterization of atypical Listeria monocytogenes associated with a canine urinary tract infection

1. An antibactericidal factor in the serum of patients with urinary-tract infection could be partially purified by absorbing the serum with the homologous organism and treating the absorption complex with a weak acid buffer. 2. Material obtained in this way contained mainly immunoglobulin G. 3. Lipopolysaccharide extracted from the homologous organism was as effective as whole bacterial cells in removing the factor from patient's serum.

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Isolation and Characterization of Multidrug Resistant Enterobacteriaceae in Urine Sample of Patients Suffering from Urinary Tract Infection with Diabetes and Nephropathy

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v20i1.54036 ◽

2021 ◽

Vol 20 (1) ◽

pp. 87-93

Author(s):

Nazmul Ahsan ◽

Monzilur Rahman ◽

Md Nazrul Islam ◽

Anwarul Azim Akhand

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Diabetic Patients ◽

Isolation And Characterization ◽

Alternative Drug ◽

Enterobacter Asburiae

Multidrug-resistant (MDR) organisms are spreading widely and becoming an issue of utmost importance to deal with. In the current study, ten urine samples from diabetic patients suffering from multiple complications, including urinary tract infection (UTI) and nephropathy were investigated. Antibiogram assays of the bacterial isolates from collected samples demonstrated resistance against most of the antibiotics tested. Further studies were conducted to determine the types of resistant bacteria that caused UTI. Analyzing the 16S rDNA sequence and phylogenetic tree, 3 isolates were identified as Escherichia coli, 5 as Klebsiella pneumoniae and the rest 2 as Enterobacter asburiae. The findings of this research indicate the necessity of urgent attention to find an effective alternative drug for treating infections caused by these resistant isolates. Dhaka Univ. J. Pharm. Sci. 20(1): 87-93, 2021 (June)

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Isolation and Characterization of Pathogens Responsible for Urinary Tract Infection in Bangladesh and Determination of their Antibiotic Susceptibility Pattern.

Journal of Applied Pharmaceutical Science ◽

10.7324/japs.2016.60410 ◽

2016 ◽

pp. 072-076 ◽

Cited By ~ 1

Author(s):

Saima Mollick ◽

Tumpa Dasgupta ◽

Md. Hasnain ◽

Mushtaque Ahmed

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Antibiotic Susceptibility ◽

Susceptibility Pattern ◽

Antibiotic Susceptibility Pattern ◽

Isolation And Characterization

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Isolation and Characterization of Antibiotic-Resistant Bacteria from Urinary Tract Infection on Diabetic Mellitus Patients

Journal of Scientific Research ◽

10.3329/jsr.v12i3.44368 ◽

2020 ◽

Vol 12 (3) ◽

pp. 391-396

Author(s):

M. Kumar ◽

L. Prince

Keyword(s):

Diabetes Mellitus ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Resistant Bacteria ◽

Klebsiella Aerogenes ◽

Diabetic Mellitus ◽

Antibiotic Resistant ◽

Isolation And Characterization

Diabetes mellitus is a chronic disease-which occurs when the pancreas does not produce enough insulin, or when the body cannot effectively use the insulin it produces. This leads to an increased concentration of glucose in the blood (hyperglycemia). Diabetes mellitus is one of the most challenging health problems in India. The present study was investigated for isolation and characterization of antibiotic-resistant bacteria from urinary tract infection on diabetic mellitus patients. The 118 diabetic urine samples were collected and UTI bacteria using HiChrome UTI Agar was isolated. The diabetic UTI isolates were confirmed as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus saprophyticus, Proteus mirabilis and Klebsiella aerogenes based on morphological and biochemical characteristics. Our study identified that almost all the bacteria were highly sensitive to Cefoperazone/Sulbactam (75/30 mcg), Gentamicin (10 mcg), Meropenem (30 mcg), Piperacillin/Tazobactam (100/10 mcg) and Nitrofurantoin (100 mcg) antibiotics. Interestingly Klebsiella aerogenes alone was found to be more resistant to the entire antibiotics used in this study. The antibiotic resistant Klebsiella aerogenes is one of the biggest treats to human health, antibiotic resistance occurs naturally, but misuse of antibiotics in humans is accelerating the process.

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