Modifikasi Medium Menggunakan Saline-Water Soluble Fraction (SSF) atau Fraksi Minyak Terlarut untuk Menumbuhkan Bakteri Pendegradasi Hidrokarbon

Isolasi bakteri pendegradasi hidrokarbon memerlukan teknik yang baik dan nutrisi optimal untuk pertumbuhannya. Kendala dalam pembuatan medium dan pengamatan isolat bakteri yang mengandung hidrokarbon sering terjadi di laboratorium, sehingga dibutuhkan teknik pengembangan metode dalam proses isolasi bakteri pendegradasi hidrokarbon. Penelitian ini bertujuan untuk mengetahui dan membandingkan jumlah dan jenis koloni bakteri yang tumbuh dalam medium Zobell+saline-water soluble fraction (SSF) dibandingkan dengan medium Zobell+minyak bumi. Metode penelitian yaitu mengisolasi bakteri menggunakan medium Zobell+SSF 6 jam (A), 12 jam (B), 24 jam (C) dan sebagai kontrol adalah medium Zobell+1% v/v minyak bumi (K), perhitungan bakteri menggunakan metode TPC dan mengidentifikasi bakteri dengan alat VITEK-MS. Hasil penelitian memperlihatkan bahwa jumlah bakteri yang tumbuh pada medium Zobell+SSF 24 jam (C) adalah 6.9 x 108 CFU/ml, hal ini menunjukkan lebih baik dibandingkan dengan Zobell+1% v/v minyak bumi (K)=5.2 x 108 CFU/ml, medium Zobell+SSF 12 jam (B)=6.6 x 107 CFU/ml dan medium Zobell+SSF 6 jam (A)=1.8 x 107 CFU/ml.Kesimpulan penelitian ini adalah bahwa dari segi jumlah total bakteri medium modifikasi Zobell+SSF pengadukan selama 24 jam (C) lebih baik dalam menumbuhkan bakteri pendegradasi hidrokarbon dibandingkan dengan pengadukan 6 jam (A), 12 jam (B), dan medium Zobell+1% v/v minyak bumi (K). Sedangkan apabila berdasarkan dengan keragaman bakteri, didapatkan hasil bahwa strain bakteri yang terisolasi pada medium modifikasi Zobell+SSF perlakuan pengadukan 6, 12, 24 jam sama dengan strain bakteri yang tumbuh pada kontrol (medium Zobell +1% v/v minyak bumi. Bakteri yang teridentifikasi sebagai bakteri pendegaradsi hidrokarbon adalah bakteri Klebsiella pneumoniae, Enterobacter asburiae/Enterobacter cloacae dan Pseudomonas aeruginosa.

The Role of Phyllosphere Bacteria in Improving Cotton Growth and Yield Under Drought Conditions

Cotton is a valuable fiber and cash crop in Telangana, India. This study examines how crop growth and fiber yield are affected by the uneven distribution of rainfall. Cotton phyllosphere bacterial isolates were gathered from the Rangareddy and Warangal Districts of Telangana, and in total, 31 phyllosphere bacterial isolates were obtained. These isolates were screened for drought tolerance and it was found that fifteen suitable isolates grew at −1.25 Mpa stress level with copious amounts of exopolysaccharides production. These isolates were further screened for ACC deaminase production and we observed 0.13–0.40 mM of α-ketobutyrate per milligram of cellular protein per hour. Five efficient bacterial isolates, namely Pseudomonas stutzeri, Acinetobacter sp., Bacillus mojavensis, Pseudomonas chlororaphis, and Enterobacter asburiae were found to produce ACC deaminase and were able to grow at −1.25 Mpa stress level. The cotton variety ADB-542 (drought susceptible) was treated with drought-tolerant five isolates. Acinetobacter sp. treated seeds had the highest seed germination and seedling vigor of 76.67 and 45.81%, respectively. The nutrient status of inoculated plots was considerably improved. The root length, fresh weight, proline content, and the number of bolls were increased by 28.52, 41.9, 28.78, and 12.99%, respectively, with the inoculation of Acinetobacter sp., to plants at −0.75 Mpa water potential. Overall the performance of cotton was improved significantly with the inoculation of phyllosphere bacteria to seeds; hence they can be recommended for the application of field crops as bio-inoculants.

Enterobacter Asburea in Intravenous Infection in Neonatal Born

Introduction: Enterobacter asburiae (E. asburiae) is a facultative anaerobic rarely isolated in neonatal care; the nosocomial infections continue to be a serious problem, associated with increased mortality rates, immediate and long-term morbidity, prolonged hospital stays, and increased cost of care, because of resistant of this specie. Objective: it’s un nosocomial infection of blood, that the first time it’s determined in neonatal hospitalization CHU Mohamed VI Marrakech. Observation: We report in this subject a case of a preterm baby had a septicemia secondary to enterobacter asburea it’s the first time this mirogerme founded in CHU, resulting from contaminated intravenous fluid hospitalized in neonatal care unit, in CHU Mohamed VI Marrakech Conclusion: the case reported in this work pushes us to deepen investigations concerning the resistance and the clinical evolution of the affected patients.

Re-examining the association of AmpC variants with Enterobacter species in the context of updated taxonomy

We performed whole genome sequencing for 17 Enterobacter clinical strains and analyzed all available Enterobacter genomes and its closely-related genera (n=3,389) from NCBI. The exact origin of plasmid-borne bla CMH and bla MIR genes is Enterobacter cloacae and Enterobacter roggenkampii , respectively, while plasmid-borne bla ACT genes originated from multiple other Enterobacter species including Enterobacter xiangfangensis , Enterobacter hoffmannii , and Enterobacter asburiae , Enterobacter ludwigii , and Enterobacter kobei . The genus of Enterobacter represents a large reservoir of plasmid-borne AmpC β-lactamase.

Draft Genome Sequence of Enterobacter asburiae NCR1, a Plant Growth-Promoting Rhizobacterium Isolated from a Cadmium-Contaminated Environment

Enterobacter asburiae NCR1 is a plant growth-promoting rhizobacterium isolated from the rhizosphere of Carpobrotus rossii . We report the draft genome sequence of E. asburiae strain NCR1, which revealed many genes facilitating beneficial interactions with plant hosts.

Characterization of Emerging Pathogens Carrying blaKPC-2 Gene in IncP-6 Plasmids Isolated From Urban Sewage in Argentina

Untreated wastewater is a reservoir for multidrug-resistant bacteria, but its role in the spread of antibiotic resistance in the human population remains poorly investigated. In this study, we isolated a KPC-2-producing ST2787 Klebsiella quasipneumoniae subsp. quasipneumoniae (WW14A), recovered from raw sewage at a wastewater treatment plant in Argentina in 2018 and determined its complete genome sequence. Strain WW14A was resistant to all β-lactams, ciprofloxacin and amikacin. A core genome phylogenetic analysis indicated that WW14A was closely related to a GES-5-producing Taiwanese strain isolated from hospital wastewater in 2015 and it was clearly distinct from strains isolated recently in Argentina and Brazil. Interestingly, blaKPC-2 was harbored by a recently described IncP-6 broad-spectrum plasmid which was sporadically reported worldwide and had never been reported before in Argentina. We investigated the presence of the IncP-6 replicon in isolates obtained from the same sampling and found a novel non-typable/IncP-6 hybrid plasmid in a newly assigned ST1407 Enterobacter asburiae (WW19C) also harboring blaKPC-2. Nanopore sequencing and hybrid assembly of strains WW14A and WW19C revealed that both IncP-6 plasmids shared 72% of coverage (~20 kb), with 99.99% of sequence similarity and each one also presented uniquely combined regions that were derived from other plasmids recently reported in different countries of South America, Asia, and Europe. The region harboring the carbapenem resistance gene (~11 kb) in both plasmids contained a Tn3 transposon disrupted by a Tn3-ISApu-flanked element and the core sequence was composed by ΔISKpn6/blaKPC-2/ΔblaTEM-1/ISKpn27. Both strains also carried genes conferring resistance to heavy metals (e.g., arsenic, mercury, lead, cadmium, copper), pesticides (e.g., glyphosate), disinfectants, and several virulence-related genes, posing a potential pathogenic risk in the case of infections. This is the first study documenting blaKPC-2 associated with IncP-6 plasmids in K. quasipneumoniae and Enterobacter cloacae complex from wastewater in Argentina and highlights the circulation of IncP-6 plasmids as potential reservoirs of blaKPC-2 in the environment.

Evaluation of the efficacy and optimization of indigenous microbial isolate for herbicides (Paraquat Dichloride, Glyphosate, and Glyphosate isopropylamine) degradation

The presence of herbicides in soil is a serious problem for the environment. Studies on degradation of Herbicide (Paraquate dichloride (PD), Rake out (RO) and Gobara (GB)) by bacteria and fungi species isolated from soil environment in Keffi Metropolis Nigeria were carried out. A total of twenty (20) soil samples were collected. The bacteria and fungi were isolated from the soil and identified using standard microbiological methods. The herbicides utilization was determined using Atomic Adsorption Spectrometer. The effect of temperature on utilization of the herbicides by Enterobacter asburiae at 26OC ranges from 1.23±0.11 mg/ml for PD, 1.14±0.29 mg/ml for RO and 0.53±0.86mg/ml for GB, Pseudomonas aeruginosa utilization ranges from 1.45±0.17 mg/ml for PD, 1.17±0.35 mg/ml, for RO 1.12± 0.82mg/ml for GB. Aspergillus flavus ranges from 2.12±0.19 mg/ml for PD, 2.00±0.03 mg/ml for RO and 2.02±0.57 mg/ml for GB, Fusarium redolens were 2.19±0.26 mg/ml for PD, 2.15±0.08 mg/ml for RO and 1.92±0.16 mg/ml for GB. Effect of incubation time on microbial herbicides degradations: for E. asburiae on PD it ranges from day 1 with 0.24±0.37 mg/ml to day 20 with 2.06±0.11 mg/ml. for P. aeruginosa on PD ranges from day 1 with 0.38±0.08 mg/ml to day 20 with 2.39±1.45 mg/ml. The Utilization of herbicides by A. flavus on PD ranges from day 1 with 0.10±0.01 mg/ml to day 20 with 2.29±0.12 mg/ml. for F. redolens in PD, it ranges from day 1 with 0.27±0.08 mg/ml to day 20 with 2.57±0.27mg/ml. The process of degradation of herbicide has become very attractive as it allows for removal of herbicide over a relatively broad range of pH and temperature

Complete Genome Sequence of Enterobacter asburiae Strain AEB30, Determined Using Illumina and PacBio Sequencing

The complete genome sequence of Enterobacter asburiae strain AEB30 is presented. The strain was isolated from store-bought ginger in Albany, CA, in 2016.

Molecular and phylogenic identifications of potential herbicide degrading microorganisms from contaminated farmland in Keffi, Nasarawa State, Nigeria

Background: Over the past years, the continuous use of herbicides has raised increasing concern mainly due to their massive pollution of the environment. To address this problem, the herbicide-degrading microorganism might be very promising. Method: In the present study, a total of twenty (20) soil samples were collected, bacteria and fungi were isolated from the soil and identified using standard microbiological and molecular studies. Results: The results revealed that a total of 2 bacterial (Enterobacter asburiae and Pseudomonas aeruginosa) and 2 fungi (Aspergillus flavus and Fusarium redolens) strains were isolated from the soil samples collected from herbicide contaminated soil in Keffi, Nasarawa State, Nigeria. The E. asburiae and P. aeruginosa had 75.0%, and 100% occurrence while the fungi isolates including the A. flavus and F. redolens had 75.0%, and 100% occurrence respectively. The 16S rDNA and ITS1F analysis confirmed the identity of the bacteria and fungi genus. Phylogenetic analysis suggested the bacteria strains were closely related to Pseudomonas aeruginosa strain CIFRI DTSB1 and Enterobacter sp revealed a close relatedness with Enterobacter asburiae RD-DAROS-04 strain, whereas, the fungi strain revealed a closely relatedness of Fusarium redolens strainTIST190421511. Conclusion: In conclusion, the microbial strains including Pseudomonas aeruginosa strain CIFRI DTSB1, Entrobacter asburiae RD-DAROS-04, and Fusarium redolens strainTIST190421511 were the major microbial habitant of pesticide-contaminated farmland in Keffi, and thus represent the herbicide degrading microorganism in this region. Further studies on the herbicide degrading properties of these microbial strains are underway.