scholarly journals miR-27a-3p Functions as a Tumor Suppressor and Regulates Non-Small Cell Lung Cancer Cell Proliferation via Targeting HOXB8

2019 ◽  
Vol 18 ◽  
pp. 153303381986197 ◽  
Author(s):  
Xiaohong Yan ◽  
Hui Yu ◽  
Yao Liu ◽  
Jie Hou ◽  
Qiao Yang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Tumor Suppressor ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Normal Cell ◽  
Cancer Cell Proliferation ◽  
Lung Cancer Cell ◽  
Normal Cell Line

MicroRNA-27a-3p has been implicated to play crucial roles in human cancers. However, the biological role and underlying mechanisms of microRNA-27a-3p in regulating nonsmall lung cancer remain unclear. MicroRNA-27a-3p expression levels in non-small lung cancer cell lines were detected by quantitative real-time polymerase chain reaction, using a normal cell line as control. The effects of microRNA-27a-3p on cell proliferation and apoptosis were analyzed by Cell Counting Kit-8 assay and flow cytometry assay. Luciferase activity reporter assay and Western blot were conducted to validate the potential targets of miR27a-3p after preliminary screening by TargetScan. Effect of microRNA-27a-3p or homeobox B8 on the overall survival of patients with non-small lung cancer was analyzed at Kaplan-Meier Plotter website. MicroRNA-27a-3p expression levels were significantly reduced in non-small lung cancer cell lines compared with normal cell line. Overexpression of microRNA-27a-3p inhibits non-small lung cancer cell proliferation but promotes cell apoptosis. Homeobox B8 was further validated as a functional target of microRNA-27a-3p. Collectively, our results indicated that microRNA-27a-3p acts as a tumor suppressor in non-small lung cancer via targeting homeobox B8.

miR-4326 promotes lung cancer cell proliferation through targeting tumor suppressor APC2

2017 ◽  
Vol 443 (1-2) ◽  
pp. 151-157 ◽  
Author(s):  
Guopeng Xu ◽  
Zhongwei Zhang ◽  
Li Zhang ◽  
Ying Chen ◽  
Ning Li ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Tumor Suppressor ◽  
Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Lung Cancer Cell

scholarly journals A novel long non-coding RNA LSAMP-1 is down-regulated in non-small cell lung cancer and predicts a poor prognosis

2020 ◽  
Author(s):  
wei gong ◽  
Fuman Qiu ◽  
Yinyan Li ◽  
Lei Yang ◽  
Yuanyuan Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Tumor Suppressor ◽  
Small Cell Lung Cancer ◽  
Cancer Cell ◽  
Poor Prognosis ◽  
Small Cell ◽  
Cancer Cell Proliferation ◽  
Lung Cancer Cell ◽  
Small Cell Lung

Abstract Background There is very little known about how long non-coding RNAs (lncRNAs) are associated with membrane proteins in lung cancer. The limbic system-associated membrane protein ( LSAMP ) has been reported to play a tumor suppressor role in a variety of cancers.Methods We aimed to explore the lncRNA associated with LSAMP and explore its effects on lung cancer.Results We found that Lnc-LSAMP-1 was significantly down-regulated in 170 cases of lung tumor tissues when compared to the adjacent non-cancerous tissues (p<0.001). Our results indicated that low expression of Lnc-LSAMP-1 was significantly correlated with stage (TNM)(p=0.006), N status (p=0.009) and poor prognosis (p=0.004). Further investigation showed that overexpression of Lnc-LSAMP-1 significantly inhibited lung cancer cell proliferation, viability, invasion and migration ability, arrested cell cycle and facilitated apoptosis. Meanwhile, the expression of Lnc-LSAMP-1 is highly correlated with the expression of nearby tumor suppressor gene LSAMP in our samples (r=0.7074, p<0.001) and TCGA database (r=0.78, p<0.001). It was found that overexpression of Lnc-LSAMP-1 can slow down the degradation rate of LSAMP gene by mRNA protection experiments. By knocking down of LSAMP gene, it was found that overexpressed Lnc-LSAMP-1 cells showed a high proliferation rate. Chemotherapy sensitization experiments showed that overexpression of Lnc-LSAMP-1 enhanced TKI inhibition of lung cancer cell proliferation, which is probably related to affecting the prognosis of patients.Conclusions Consequently, the above data suggested that Lnc-LSAMP-1 functions as a tumor suppressor and provides a new potentially therapeutic and prognostic target for non-small cell lung cancer.

scholarly journals Long non-coding RNA FGD5-AS1 promotes non-small cell lung cancer cell proliferation through sponging hsa-miR-107 to up-regulate FGFRL1

2020 ◽  
Vol 40 (1) ◽  
Author(s):  
Yafeng Fan ◽  
Hongxia Li ◽  
Zhongping Yu ◽  
Wen Dong ◽  
Xiaoyan Cui ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Small Cell Lung Cancer ◽  
Cancer Cell ◽  
Small Cell ◽  
Cancer Cell Proliferation ◽  
Lung Cancer Cell ◽  
Small Cell Lung ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Abstract Long non-coding RNA (lncRNA) FYVE, RhoGEF and PH domain containing 5 antisense RNA 1 (FGD5-AS1) has been reported as an oncogene in colorectal cancer, promoting its tumorgenesis. The present paper focused on searching the potential function of FGD5-AS1 in non-small cell lung carcinoma (NSCLC). There are connections between the expression of lncRNA FGD5-AS1 and human NSCLC tumor growth and progression. Also, the relationships between FGD5-AS1, hsa-miR-107 and mRNA fibroblast growth factor receptor like 1 (FGFRL1) are going to test their interaction in NSCLC cell lines, which may cause a series of biological behaviors of NSCLC cells. qRT-PCR analysis was conducted to test the expression of RNAs in different situation. CCK-8 experiment and clone formation assay were performed to assess proliferation of NSCLC cells. Also, connection between FGD5-AS1 and hsa-miR-107 were investigated by luciferase reporter assay and RNA pull-down assay. Rescue experiments were performed to verify the modulating relationship between FGD5-AS1, hsa-miR-107 and FGFRL1. High-level expression of FGD5-AS1 was found in NSCLC. FGD5-AS1 may promote the proliferation of NSCLC cells. Also, the combination between hsa-miR-107, FGD5-AS1 and NSCLC have been proved, which means they can play an interaction function in NSCLC cells. Thence, we concluded that lncRNA FGD5-AS1 promotes non-small cell lung cancer cell proliferation through sponging hsa-miR-107 to up-regulate FGFRL1.

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