DYNLT3 Is Required for Chromosome Alignment During Mouse Oocyte Meiotic Maturation

UCHL5IP is one of the subunits of the haus complex, which is important for microtubule generation, spindle bipolarity and accurate chromosome segregation in Drosophila and human mitotic cells. In this study, the expression and localisation of UCHL5IP were explored, as well as its functions in mouse oocyte meiotic maturation. The results showed that the UCHL5IP protein level was consistent during oocyte maturation and it was localised to the meiotic spindle in MI and MII stages. Knockdown of UCHL5IP led to spindle defects, chromosome misalignment and disruption of γ-tubulin localisation in the spindle poles. These results suggest that UCHL5IP plays critical roles in spindle formation during mouse oocyte meiotic maturation.

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Bisphenol B Exposure Disrupts Mouse Oocyte Meiotic Maturation in vitro Through Affecting Spindle Assembly and Chromosome Alignment

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2020.616771 ◽

2020 ◽

Vol 8 ◽

Author(s):

Shou-Xin Zhang ◽

Zhi-Ming Ding ◽

Muhammad Jamil Ahmad ◽

Yong-Sheng Wang ◽

Ze-Qun Duan ◽

...

Keyword(s):

Dna Damage ◽

Spindle Assembly ◽

Epigenetic Modifications ◽

Mouse Oocyte ◽

Meiotic Maturation ◽

Meiotic Arrest ◽

First Polar Body ◽

Chromosome Alignment ◽

Oocyte Meiotic Maturation

Bisphenol B (BPB), a substitute of bisphenol A (BPA), is widely used in the polycarbonate plastic and resins production. However, BPB proved to be not a safe alternative to BPA, and as an endocrine disruptor, it can harm the health of humans and animals. In the present study, we explored the effects of BPB on mouse oocyte meiotic maturation in vitro. We found that 150 μM of BPB significantly compromised the first polar body extrusion (PBE) and disrupted the cell cycle progression with meiotic arrest. The spindle assembly and chromosome alignment were disordered after BPB exposure, which was further demonstrated by the aberrant localization of p-MAPK. Also, BPB exposure increased the acetylation levels of α-tubulin. As a result, the spindle assemble checkpoint (SAC) was continuously provoked, contributing to meiotic arrest. We further demonstrated that BPB severely induced DNA damage, but the ROS and ATP production were not altered. Furthermore, the epigenetic modifications were changed after BPB exposure, as indicated by increased K3K9me3 and H3K27me3 levels. Besides, the pattern of estrogen receptor α (ERα) dynamics was disrupted with a mass gathering on the spindle in BPB-exposed oocytes. Our collective results indicated that exposure to BPB compromised meiotic maturation and damaged oocyte quality by affecting spindle assembly and chromosome alignment, acetylation of α-tubulin, DNA damage, epigenetic modifications, and ERα dynamics in mouse oocytes.

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Perturbation of survivin expression affects chromosome alignment and spindle checkpoint in mouse oocyte meiotic maturation

Cell Cycle ◽

10.4161/cc.8.20.9855 ◽

2009 ◽

Vol 8 (20) ◽

pp. 3365-3372 ◽

Cited By ~ 25

Author(s):

Shao-Chen Sun ◽

Liang Wei ◽

Mo Li ◽

Sheng-Li Lin ◽

Bao-Zeng Xu ◽

...

Keyword(s):

Spindle Checkpoint ◽

Survivin Expression ◽

Mouse Oocyte ◽

Meiotic Maturation ◽

Chromosome Alignment ◽

Oocyte Meiotic Maturation

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EB1 Is Essential for Spindle Formation and Chromosome Alignment During Oocyte Meiotic Maturation in Mice

Microscopy and Microanalysis ◽

10.1017/s1431927620024897 ◽

2021 ◽

pp. 1-7

Author(s):

Dongjie Zhou ◽

Zheng-Wen Nie ◽

Xiang-Shun Cui

Keyword(s):

Cell Shape ◽

Control Cell ◽

Meiotic Maturation ◽

Junctional Complex ◽

Spindle Formation ◽

Oocyte Meiosis ◽

Polarized Cell Growth ◽

Chromosome Alignment ◽

Oocyte Meiotic Maturation

The cytoskeleton plays an orchestrating role in polarized cell growth. Microtubules (MTs) not only play critical roles in chromosome alignment and segregation but also control cell shape, division, and motility. A member of the plus-end tracking proteins, end-binding protein 1 (EB1), regulates MT dynamics and plays vital roles in maintaining spindle symmetry and chromosome alignment during mitosis. However, the role of EB1 in mouse oocyte meiosis remains unknown. Here, we examined the localization patterns and expression levels of EB1 at different stages. EB1 protein level was found to be stable during meiosis. EB1 mainly localized along the spindle and had a similar localization pattern as that of α-tubulin. The EB1 protein was degraded with a Trim-Away method, and the results were further confirmed with western blotting and immunofluorescence. At 12 h of culture after EB1 knockdown (KD), a reduced number of mature MII oocytes were observed. EB1 KD led to spindle disorganization, chromosome misalignment, and missegregation; β-catenin protein binds to actin via the adherens junctional complex, which was significantly reduced in the EB1 KD oocytes. Collectively, we propose that the impairment of EB1 function manipulates spindle formation, thereby promoting chromosomal loss, which is expected to fuel aneuploidy and possibly fertilization failure.

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Maternal mRNAs with distinct 3′ UTRs define the temporal pattern ofCcnb1synthesis during mouse oocyte meiotic maturation

Genes & Development ◽

10.1101/gad.296871.117 ◽

2017 ◽

Vol 31 (13) ◽

pp. 1302-1307 ◽

Cited By ~ 27

Author(s):

Ye Yang ◽

Cai-Rong Yang ◽

Seung Jin Han ◽

Enrico Maria Daldello ◽

Ara Cho ◽

...

Keyword(s):

Temporal Pattern ◽

Mouse Oocyte ◽

Meiotic Maturation ◽

Maternal Mrnas ◽

Oocyte Meiotic Maturation

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JNK2 Participates in Spindle Assembly during Mouse Oocyte Meiotic Maturation

Microscopy and Microanalysis ◽

10.1017/s1431927610094456 ◽

2011 ◽

Vol 17 (2) ◽

pp. 197-205 ◽

Cited By ~ 10

Author(s):

Xin Huang ◽

Jing-Shan Tong ◽

Zhen-Bo Wang ◽

Cai-Rong Yang ◽

Shu-Tao Qi ◽

...

Keyword(s):

Polar Body ◽

Specific Inhibitor ◽

Germinal Vesicle ◽

Spindle Assembly ◽

Mouse Oocyte ◽

Meiotic Maturation ◽

Cytoplasmic Microtubule ◽

Spindle Poles ◽

First Polar Body ◽

Oocyte Meiotic Maturation

AbstractIt is well known that c-Jun N-terminal kinase (JNK) plays pivotal roles in various mitotic events, but its function in mammalian oocyte meiosis remains unknown. In this study, we found that no specific JNK2 signal was detected in germinal vesicle stage. JNK2 was associated with the spindles especially the spindle poles and cytoplasmic microtubule organizing centers at prometaphase I, metaphase I, and metaphase II stages. JNK2 became diffusely distributed and associated with the midbody at telophase I stage. Injection of myc-tagged JNK2α1 mRNA into oocytes also revealed its localization on spindle poles. The association of JNK2 with spindle poles was further confirmed by colocalization with the centrosomal proteins, γ-tubulin and Plk1. Nocodazole treatment showed that JNK2 may interact with Plk1 to regulate the spindle assembly. Then we investigated the possible function of JNK2 by JNK2 antibody microinjection and JNK specific inhibitor SP600125 treatment. These two manipulations caused abnormal spindle formation and decreased the rate of first polar body (PB1) extrusion. In addition, inhibition of JNK2 resulted in impaired localization of Plk1. Taken together, our results suggest that JNK2 plays an important role in spindle assembly and PB1 extrusion during mouse oocyte meiotic maturation.

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