scholarly journals Cytotoxicity, Antimicrobial Activity and Composition of Essential Oil from Tanacetum balsamita L. Subsp. Balsamita

2009 ◽  
Vol 4 (1) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Morteza Yousefzadi ◽  
Samad Nejad Ebrahimi ◽  
Ali Sonboli ◽  
Farah Miraghasi ◽  
Shahla Ghiasi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Capillary Gc ◽  
Aerial Parts ◽  
Full Flowering ◽  
Main Components ◽  
Total Oil

The aerial parts of Tanacetum balsamita subsp. balsamita were collected at full flowering stage from Tabriz, Iran. The essential oil was isolated by hydrodistillation and analyzed by combination of capillary GC and GC-MS. Twenty seven components were identified, representing 99.2% of the total oil. Carvone (51.0%), β-thujone (20.8%), 1,8-cineole (4.4%) and α-thujone (3.2%) were the main components. The in vitro antimicrobial activity of the essential oil of T. balsamita subsp. balsamita was studied against eight Gram-positive and Gram-negative bacteria ( Bacillus subtilis, B. pumulis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi ( Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The results of the antimicrobial activity of the essential oil according to the disc diffusion method and MIC values indicated that the oil exhibited moderate to high antimicrobial activity. The in vitro toxicological study based on the MTT cytotoxicity assay on human fetal skin fibroblast (HFSF) and Monkey kidney (Vero) cell lines showed IC50 values of 2500 and 1250 μg/mL, respectively.

Antimicrobial Activity of Essential Oil and Major Constituents of Salvia chloroleuca

2008 ◽  
Vol 63 (5-6) ◽  
pp. 337-340 ◽  
Author(s):  
Morteza Yousefzadi ◽  
Ali Sonboli ◽  
Samad Nejad Ebrahimi ◽  
Seid Hasan Hashemi
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Diffusion Method ◽  
Capillary Gc ◽  
Aerial Parts ◽  
Full Flowering ◽  
Tehran Province ◽  
Main Components ◽  
Total Oil

The aerial parts of Salvia chloroleuca were collected at full flowering stage at Shahrestanak (Tehran province of Iran). The essential oil was isolated by hydrodistillation and analyzed by combination of capillary GC and GC-MS. Thirty-four components were identified, representing 98.5% of the total oil. β-Pinene (10.6%), α-pinene (9.0%), β-caryophyllene (9.0%), 1,8-cineole (9.0%) and carvacrol (7.9%) were the main components. The in vitro antimicrobial activity of the essential oil of S. chloroleuca was studied against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger); the disc diffusion method and MIC values indicated that the oil exhibited moderate to high antimicrobial activity.

Antimicrobial Activity and Composition of the Essential Oil of Gontscharovia popovii from Iran

2006 ◽  
Vol 61 (9-10) ◽  
pp. 681-684 ◽  
Author(s):  
Ali Sonboli ◽  
Fatemeh Sefidkon ◽  
Morteza Yousefzadi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Gram Negative Bacteria ◽  
Capillary Gc ◽  
Aerial Parts ◽  
Full Flowering ◽  
Strong Antimicrobial Activity

AbstractThe aerial parts of Gontscharovia popovii (B. Fedtsch. and Gontsch.) Boriss. were collected at full flowering stage. The essential oil was isolated by hydrodistillation and analyzed by a combination of capillary GC and GC-MS. Thirty-one components were identified with the main constituent being carvacrol (71.9%), followed by linalool (5.5%), p-cymene (4.5%) and γ-terpinene (4.4%). The in vitro antimicrobial activity of the essential oil of G. popovii was studied against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The results of the bioassays showed that the oil exhibited strong antimicrobial activity against all the tested fungi and bacteria except for the resistant bacterium Pseudomonas aeruginosa.

scholarly journals Composition and in vitro antimicrobial activity of the essential oil of Dorema ammoniacum D. Don. fruit from Iran

2011 ◽  
Vol 76 (6) ◽  
pp. 857-863 ◽  
Author(s):  
Morteza Yousefzadi ◽  
Hossein Mirjalili ◽  
Naba Alnajar ◽  
Amineh Zeinali ◽  
Mitra Parsa
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
In Vitro Antimicrobial Activity ◽  
Main Components ◽  
Total Oil ◽  
Antimicrobial Assay

The genus Dorema (Apiaceae) is represented in the flora of Iran with seven species of which two, D. ammoniacum D. Don. and D. aucheri Boiss. are endemic. Ripe fruits of D. ammoniacum collected just in the deciduous time were subjected to hydrodistillation to yield the essential oil, which was subsequently analyzed by GC and GC-MS. Twenty-nine compounds were identified and quantified, representing 95.1 % of the total oil. (Z)-Ocimenone (22.3 %) and (E)-ocimenone (18.1 %) were the main components of the oil. In vitro antimicrobial activity of the oil was evaluated against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The results of the antimicrobial assay of the oil by the disc diffusion method and the MIC values indicated that the oil exhibited moderate to high antimicrobial activity, especially against B. subtilis and S. epidermidis with MIC value of 3.75 mg ml-1.

scholarly journals Chemical Composition and Biological Activity of the Essential Oil from Jamaican Cleome serrata

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Megil J. McNeil ◽  
Roy B. R. Porter ◽  
Lawrence A. D. Williams
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Oil Composition ◽  
Filter Paper Disc ◽  
Aerial Parts ◽  
Main Components ◽  
Total Oil

The chemical composition of the essential oil obtained from the aerial parts of Cleome serrata by hydrodistillation was analyzed by employing GC-FID, GC-MS and RI. Fourteen compounds comprising 90.4% of the total oil composition were characterized. The main components identified were ( Z)-phytol (53.0%) and di(2-ethylhexyl)-phthalate (DEHP) (14.7%). The oil was evaluated for its in vitro antimicrobial activities against nine pathogenic microorganisms using the filter paper disc diffusion method. Moderate antimicrobial activity was observed against five of the pathogens assayed. In addition, the essential oil was tested against the sweet potato weevil, Cylas formicarius elegantulus. Strong knockdown insecticidal activity was observed.

Antimicrobial Activity of Some Salvia Species Essential Oils from Iran

2007 ◽  
Vol 62 (7-8) ◽  
pp. 514-518 ◽  
Author(s):  
Morteza Yousefzadi ◽  
Ali Sonboli ◽  
Farah Karimi ◽  
Samad Nejad Ebrahimi ◽  
Behvar Asghari ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Staphylococcus Epidermidis ◽  
Diffusion Method ◽  
Capillary Gc ◽  
Aerial Parts ◽  
Full Flowering ◽  
Weak Activity

The aerial parts of Salvia multicaulis, S. sclarea and S. verticillata were collected at full flowering stage. The essential oils were isolated by hydrodistillation and analyzed by combination of capillary GC and GC-MS. The in vitro antimicrobial activity of the essential oils were studied against eight Gram-positive and Gram-negative bacteria (Bacillus subtilis, Bacillus pumulis, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The results of antibacterial activity tests of the essential oils according to the disc diffusion method and MIC values indicated that all the samples have moderate to high inhibitory activity against the tested bacteria except for P. aeruginosa which was totally resistant. In contrast to antibacterial activity, the oils exhibited no or slight antifungal property, in which only the oil of S. multicaulis showed weak activity against two tested yeasts, C. albicans and S. cerevisiae

scholarly journals Biological Activity and Composition of the Essential Oil of Tetrataenium Nephrophyllum (Apiaceae) from Iran

2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Ali Sonboli ◽  
Mohammad Reza Kanani ◽  
Morteza Yousefzadi ◽  
Mehran Mojarrad
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Gram Negative Bacteria ◽  
Flowering Stage ◽  
High Antimicrobial Activity ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D ◽  
Aliphatic Esters ◽  
Total Oil

The aerial parts of Tetrataenium nephrophyllum were collected at the flowering stage, hydrodistilled, and the essential oil was analyzed by GC and GC-MS. Forty components accounting for 97.9% of the total oil were identified. Germacrene D (38.5%), 2-ethylhexyl acetate (11.2%), n-octyl 2-methylbutanoate (9.2%) and geranyl isovalerate (8.3%) were the major constituents. Sesquiterpene hydrocarbons (51.3%) and aliphatic esters (40.4%) were found to be the main group of compounds. The antimicrobial activity of the essential oil of T. nephrophyllum was determined against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae), as well as three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The bioassay showed that the oil exhibited moderate to high antimicrobial activity.

Antimicrobial activity of Thymus longicaulis C. Presl essential oil against respiratory pathogens

2012 ◽  
Vol 7 (6) ◽  
pp. 1109-1115 ◽  
Author(s):  
Sanda Vladimir-Knežević ◽  
Ivan Kosalec ◽  
Marija Babac ◽  
Marinko Petrović ◽  
Jovica Ralić ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Perennial Herb ◽  
Diffusion Method ◽  
Respiratory Pathogens ◽  
Antimicrobial Drugs ◽  
Plant Parts ◽  
Traditional Remedy ◽  
Main Components

AbstractThymus longicaulis C. Presl is a small aromatic perennial herb used as a traditional remedy for cold, flu and cough. Composition of the essential oil of T. longicaulis from Croatia and its in vitro antimicrobial activity against the most common respiratory pathogens were evaluated. The yield of essential oil obtained by hydrodistillation from aerial plant parts was 1.2%. According to the GC-MS analysis, a total of forty one compounds (99%) were identified. Thymol (46.3%), γ-terpinene (16.2%), thymyl methyl ether (11.4%), and p-cymene (9.4%) were the main components. Antimicrobial activity of the essential oil against six clinically isolated bacterial and yeast strains was determined using standard disc agar diffusion method and microdilution broth assay. The essential oil exhibited antimicrobial activity towards all tested respiratory pathogens. The most sensitive strains were Haemophilus influenzae and Streptococcus pneumoniae(MIC=0.78 mg/mL), while Staphylococcus aureus was the most resistant (MIC>25.00 mg/mL). Our results indicate that T. longicaulis essential oil could be effective against clinically relevant respiratory pathogens which have the ability to develop resistance to antimicrobial drugs.

scholarly journals Chemical composition and antimicrobial activity of essential oil from Pyrethrum pulchrum Ledeb.

2018 ◽  
Vol 19 (45) ◽  
pp. 38-43 ◽  
Author(s):  
Uugangerel Erdenetsogt ◽  
Choijamts Gotov ◽  
Kerstin Voigt ◽  
Stefan Bartram ◽  
Wilhelm Boland ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Oil Composition ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Total Oil ◽  
Sporidiobolus Salmonicolor

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Pyrethrum pulchrum Ledeb. were investigated. Dried plant material was hydro-distillated yielding 0.1% of essential oil. The oil was analyzed by GC-MS techniques. Fifty-five compounds were identified representing 99.7% of the total oil composition. Camphor was the predominant compound (33.9%) followed by linalool (21.1%) and α-pinene (9.0%). The antimicrobial activity of the oil was determined using the disk diffusion method against Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Mycobacterium vaccae and fungi (Candida albicans, Sporidiobolus salmonicolor and Penicillum notatum). The essential oil of P. pulchrum displays an intermediate activity against selected bacteria.

scholarly journals Biological Activity and Composition of the Essential Oil of Dracocephalum Moldavica L. Grown in Iran

2008 ◽  
Vol 3 (9) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Ali Sonboli ◽  
Mehran Mojarrad ◽  
Abbas Gholipour ◽  
Samad Nejad Ebrahimi ◽  
Mitra Arman
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Biological Activity ◽  
Essential Oil ◽  
Geranyl Acetate ◽  
Oil Analysis ◽  
Inhibition Zones ◽  
Main Components ◽  
Total Oil

The biological activity and composition of the essential oil of the aerial flowering parts of D. moldavica have been investigated. The oil analysis was performed by GC and GC-MS. Thirteen compounds were identified, representing 99.8% of the oil. Oxygenated monoterpenes (96.3%) were found to be the principal group of compounds, of which neral, geranial, geranyl acetate and geraniol with 32.1, 21.6, 19.9 and 17.6% of the total oil were the main constituents, respectively. The in vitro antimicrobial activity of the essential oil and its main components, citral, geraniol and geranyl acetate, showed that all of the tested microorganisms were highly inhibited by the essential oil with inhibition zones ranged from 15 – 41 mm for bacteria and 29 – 30 mm for fungi. The most sensitive microorganisms were Bacillus subtilis, Staphylococcus aureus and S. epidermidis with the lowest MIC values of 0.9 mg/mL. Considering sensitivity screening, it is conceivable that the activity of the oil from D. moldavica could be attributed mainly to the presence of citral.

scholarly journals Essential Oil Composition of Pimpinella cypria and its Insecticidal, Cytotoxic, and Antimicrobial Activity

2016 ◽  
Vol 11 (10) ◽  
pp. 1934578X1601101
Author(s):  
Nurhayat Tabanca ◽  
Ayse Nalbantsoy ◽  
Ulrich R. Bernier ◽  
Natasha M. Agramonte ◽  
Abbas Ali ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Positive Control ◽  
Essential Oil Composition ◽  
Amblyomma Americanum ◽  
Effective Dosage ◽  
Oil Composition ◽  
Aerial Parts ◽  
Main Components

A water-distilled essential oil (EO) from the aerial parts of Pimpinella cypria Boiss. (Apiaceae), an endemic species in northern Cyprus, was analyzed by GC-FID and GC-MS. Forty-five compounds were identified in the oil, which comprised 81.7% of the total composition. The compound classes in the oil were oxygenated sesquiterpenes (33.9%), sesquiterpenes (22.0%), monoterpenes (11.4%), oxygenated monoterpenes (2.6%), and phenylpropanoids (7.5%). The main components of the oil were ( Z)-β-farnesene (6.0%), spathulenol (5.9%), ar-curcumene (4.3%), and 1,5-epoxy-salvial(4)14-ene (3.8%). The P. cypria EO deterred yellow fever mosquitoes (Aedes aegypti) from biting at a concentration of 10 μg/cm2 in in vitro bioassays. The oil was tested for repellency in assays using human volunteers. The oil had a minimum effective dosage (MED) for repellency of 47 ± 41 μg/cm2 against Ae. aegypti, which was less efficacious than the positive control N,N-diethyl-3-methylbenzamide (DEET). In larval bioassays, P. cypria EO showed an LC50 value of 28.3 ppm against 1st instar Ae. aegypti larvae. P. cypria EO demonstrated dose dependent repellency against nymphs of the lone star tick, Amblyomma americanum. Between 45.0% and 85.0% repellency was observed at concentrations ranging from 26 to 208 μg/cm2. However, P. cypria EO was less effective compared with DEET in the tick bioassays. Cytotoxicity assays showed that the P. cypria EO did not exhibit significant effects up to the maximum treatment concentration of 50 μg/mL on HEK293, PC3, U87MG, and MCF cells. P. cypria EO also demonstrated moderate antimicrobial activity against Gram-negative and -positive bacteria with MICs ranging from 15.6 to 62.5 μg/mL, except for Candida albicans, which showed the same MIC value of 7.8 μg/mL as the positive control, flucytosine. This is the first report on the chemical composition of P. cypria EO and its insecticidal, toxicant, cytotoxic, and antimicrobial activity.

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