scholarly journals Composition, in vitro Anti-mildew Fungal Activities of the Heartwood Essential Oil of Chamaecyparis formosensis from Taiwan

2018 ◽  
Vol 13 (10) ◽  
pp. 1934578X1801301
Author(s):  
Yu-Chang Su ◽  
Kuang-Ping Hsu ◽  
Chen-Lung Ho

In this study, anti-mildew fungal activities of the heartwood essential oil, and its constituents from Chamaecyparis formosensis were evaluated in vitro against 7 mildew fungi. The main compounds responsible for the anti-mildew fungal activities were isolated and identified. The heartwood essential oil of C. formosensis was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. The heartwood oil consisted primarily of myrtenol (18.4%), cis-myrtanol (14.0%), α-muurolol (13.8%), α-cadinol (12.7%), and chamaecynone (9.8%). The heartwood oil was shown to have excellent anti-mildew fungal activities. Further fractionation of the heartwood oil produced α-cadinol, chamaecynone, α-muurolol, τ-cadinol, and τ-muurolol. The 5 compounds exhibited very strong anti-mildew fungal activities. For the anti-mildew fungal activities of the heartwood oil, the active source compounds were determined to be α-cadinol, chamaecynone, α-muurolol, τ-cadinol, and τ-muurolol.

2013 ◽  
Vol 8 (9) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yu-Chang Su ◽  
Kuan-Ping Hsu ◽  
Eugene I-Chen Wang ◽  
Chen-Lung Ho

In this study, anti-mildew and anti-wood-decay fungal activities of the leaf and fruits essential oil and its constituents from Juniperus formosana were evaluated in vitro against seven mildew fungi and four wood decay fungi, respectively. The main compounds responsible for the anti-mildew and anti-wood-decay fungal activities were also identified. The essential oil from the fresh leaves and fruits of J. formosana were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS, respectively. The leaf oil mainly consisted of α-pinene (41.0%), limonene (11.5%), α-cadinol (11.0%), elemol (6.3%), and β-myrcene (5.8%); the fruit oil was mostly α-pinene (40.9%), β-myrcene (32.4%), α-thujene (5.9%) and limonene (5.9%). Comparing the anti-mildew and anti-wood-decay fungal activities of the oils suggested that the leaf oil was the most effective. For the anti-mildew and anti-wood-decay fungal activities of the leaf oil, the active source compounds were determined to be α-cadinol and elemol.


2020 ◽  
Vol 15 (8) ◽  
pp. 1934578X2093697
Author(s):  
Kuang-Ping Hsu ◽  
Yu-Chang Su ◽  
Chen-Lung Ho

In this study, antiphytopathogenic fungi activities of the leaf and cones essential oils and its constituents from Cunninghamia lanceolata were evaluated in vitro against 6 plant pathogenic fungi. The main compounds responsible for the antiphytopathogenic fungi activities were isolated and identified. The essential oil from the fresh leaves and cones of C. lanceolata was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS, respectively. The leaf oil consisted primarily of ferruginol (10%), τ-cadinol (8.2%), and α-cadinol (6.6%); the cones oil’s main constituents were abietadiene (42.5%), abietatriene (13.1%), and α-pinene (9.6%). Comparing the antiphytopathogenic fungi activities of the oils suggested that leaf oil was the most effective one. Further fractionation of the leaf oil produced ferruginol, τ-cadinol, and α-cadinol. The 3 compounds exhibited very strong antiphytopathogenic fungi activities. For the antiphytopathogenic fungi activities of the leaf oil, the active source compounds were determined to be ferruginol, τ-cadinol, and α-cadinol.


2017 ◽  
Vol 12 (10) ◽  
pp. 1934578X1701201
Author(s):  
Yu-Chang Su ◽  
Kuang-Ping Hsu ◽  
Chen-Lung Ho

In this study, antibacterial and anti-mildew fungal activities of the twig and fruit essential oil and its constituents from Eucalyptus citriodora were investigated in vitro against eight bacteria and seven mildew fungi, respectively. The major ingredient responsible for the antibacterial and anti-mildew fungal activities were also identified. The essential oil from the fresh twigs and fruits of E. citriodora were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC–FID and GC–MS, respectively. The twig oil consisted primarily of 1,8-cineole (17.7%), p-cymene (17.1%), caryophyllene oxide (13.8%), and isopulegol (12.2%); the fruit oil's main constituent was p-cymene (23.2%), α-pinene (16.1%), α-cadinol (11.5%), and τ-cadinol (8.4%). In addition, comparing the antibacterial and anti-mildew fungal activities of the oils showed that the fruit oil was the most effective. For the antibacterial and anti-mildew fungal activities of the fruit oil, the active source compounds were determined to be τ-cadinol and τ-cadinol.


2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001
Author(s):  
Yu-Chang Su ◽  
Kuan-Ping Hsu ◽  
Shu-Ching Li ◽  
Chen-Lung Ho

This study investigated the chemical composition, in-vitro cytotoxicity, and anti-mildew fungal activities of the essential oil isolated from the leaf of Machilus thunbergii from Taiwan. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC–FID and GC–MS. Eighty-three compounds were identified, representing 99.8% of the oil. The main components identified were n-decanal (26.6%), β-caryophyllene (15.8%), α-humulene (10.8%), and β-eudesmol (10.5%). The oil exhibited cytotoxic activity against human oral, liver, lung, colon, melanoma, and leukemic cancer cells. The active source compounds were β-caryophyllene, α-humulene, and β-eudesmol. The anti-mildew activity of the leaf oil was also evaluated. Results showed that the leaf oil had excellent anti-mildew activity. For the anti-mildew activity of the leaf oil, the active source compound was determined to be β-eudesmol.


2013 ◽  
Vol 8 (1) ◽  
pp. 1934578X1300800
Author(s):  
Yu-Chang Su ◽  
Chen-Lung Ho

This study investigated the chemical composition and in-vitro cytotoxic activities of the essential oil isolated from the leaf of Beilschmiedia erythrophloia. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Fifty-five compounds were identified, representing 100% of the oil. The main components identified were β-caryophyllene (22.6%), α-humulene (21.9%), terpinen-4-ol (5.3%), cis-β-ocimene (5.1%), sabinene (5.0%) and limonene (4.5%). The anticancer activities of oil were evaluated. The results showed that the oil exhibited cytotoxic activity against human oral, liver, lung, colon, melanoma, and leukemic cancer cells.


2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yu-Chang Su ◽  
Kuan-Ping Hsu ◽  
Eugene I-Chen Wang ◽  
Chen-Lung Ho

This study investigated the chemical composition and in vitro anticancer activities of the essential oil isolated from the leaf of Neolitsea variabillima. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Sixty-seven compounds were identified, representing 100% of the oil. The main components identified were trans-β-ocimene (13.4%), α-cadinol (10.5%), terpinen-4-ol (9.3%), τ-cadinol (9.2%), β-caryophyllene (8.8%), and sabinene (6.7%). The anticancer activities of oil were evaluated. The results showed that the oil exhibited cytotoxic activity against human oral, liver, lung, colon, melanoma, and leukemic cancer cells. The presence of β-caryophyllene, τ-cadinol, and α-cadinol significantly contributed to the anticancer activities of N. variabillima leaf oil.


2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000
Author(s):  
Yu-Chang Su ◽  
Kuang-Ping Hsu ◽  
Eugene I-Chen Wang ◽  
Chen-Lung Ho

In this study, anti-mildew activities of the essential oils of the leaves, wood, twigs, flowers, and their constituents from Michelia compressa var. formosana were evaluated in vitro against 7 mildew fungi. The main compounds responsible for the anti-mildew activities were isolated and identified. The essential oils from the fresh leaves, wood, twigs, and flowers of M. compressa var. formosana were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. The essential oil yield from the flowers was the highest, and the oils obtained from the 4 parts of the tree were composed mainly of sesquiterpenoids. The leaf oil consisted primarily of α-cadinol (18.9%) and germacrene D (18.5%); the twig oil's main constituent was α-cadinol (19.1%); the wood oil's major components were α-cadinol (25.7%) and β-eudesmol (20.2%); and the flower oil contained α-cadinol (11.4%). Comparing the anti-mildew activities of the oils suggested that wood oil was the most effective. Further fractionation of the wood oil produced α-cadinol, β-eudesmol, τ-cadinol, and elemol. The 4 compounds exhibit very strong anti-mildew activities, and these in descending order are α-cadinol, elemol, β-eudesmol, and τ-cadinol. For the anti-mildew activities of the wood oil, the active source compounds were determined to be α-cadinol, elemol, β-eudesmol, and τ-cadinol.


2017 ◽  
Vol 12 (2) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Yu-Chang Su ◽  
Chen-Lung Ho

This study investigated the chemical composition, in vitro cytotoxicity, anti-mildew, and anti-wood-decay fungal activities of the essential oil isolated from the fruit of Liquidambar formosana from Taiwan. The essential oil from the fresh fruit was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. A total of 45 compounds were identified, representing 98.5% of the essential oil. The main components identified were α-pinene (16.8%), β-caryophyllene (10.1%), τ-muurolol (8.3%), τ-cadinol (7.6%), β-pinene (6.7%), and sabinene (5.7%). The essential oil exhibited cytotoxic activity against human oral, liver, and lung cancer cells. The active source compounds were β-caryophyllene, τ-cadinol, and τ-muurolol. The fruit essential oil was shown to have excellent anti-mildew and anti-wood-decay fungal activities, the active compounds being evaluated as τ-cadinol and τ-muurolol.


2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Yu-Chang Su ◽  
Kuang-Ping Hsu ◽  
Chen-Lung Ho

In this study, cytotoxicity, anti-mildew and anti-wood-decay fungal activities of the bark essential oil and its constituents from Cunninghamia lanceolata var. konishii were evaluated in vitro against three human cancer cell lines, seven mildew fungi, and four wood decay fungi, respectively. The main compounds responsible for the cytotoxicity, anti-mildew, and anti-wood decay fungal activities were also identified. The essential oil from the barks of C. lanceolata var. konishii was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS, respectively. The bark oil mainly consisted of ferruginol (15.1%), β-caryophyllene (11.2%), α-cadinol (10.9%) and cedrol (10.1%). The essential oil exhibited cytotoxic activity against human oral, liver, and lung cancer cells. The active source compounds were α-cadinol, cedrol, β-caryophyllene, and ferruginol. The bark oil was shown to have excellent anti-mildew and anti-wood-decay fungal activities, the active compounds being evaluated as α-cadinol, cedrol, and ferruginol.


2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700
Author(s):  
Chen-Lung Ho ◽  
Kuo-Feng Hua ◽  
Kuan-Ping Hsu ◽  
Eugene I-Chen Wang ◽  
Yu-Chang Su

In this study, antipathogenic activities of the twig essential oil and its constituents from Chamaecyparis formosensis Matsum were evaluated in vitro against six plant pathogenic fungi. The essential oil from the fresh twigs was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Twenty-five compounds were identified, representing 98.9% of the oil. The main components were β-eudesmol (25.1%), τ-muurolol (21.6%), elemol (15.0%), totarol (14.9%), and α-cadinol (12.4%). The twig oil (500 μg/mL) showed growth inhibitory activity against the phytopathogenic fungi, Fusarium oxysporum, Pestalotiopsis funereal, and Ganoderma austral, with antifungal indices of 92.7%, 71.1%, and 87.7%, respectively. In addition, the oil suppressed totally the growth of Rhizoctonia solani, Colletotrichum gloeosporioides, and Fusarium solani. In order to ascertain the source compounds of these antipathogenic activities, the main components were individually evaluated. τ-Muurolol and α-cadinol exhibited excellent activity against F. oxysporum, R. solani, C. gloeosporioides, and F. solani, with IC50< 50 μg/mL. These compounds also efficiently inhibited the mycelial growths of P. funereal and G. austral. Thus, α-cadinol and τ-muurolol could be considered as potential natural fungicides for controlling fungal pathogens and worth.


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