scholarly journals THE HISTOCHEMICAL DEMONSTRATION OF POLYADENYLIC ACID HYDROLASES IN RAT LIVER DURING AZO DYE CARCINOGENESIS

1972 ◽  
Vol 20 (7) ◽  
pp. 536-541 ◽  
Author(s):  
R. DAOUST
Keyword(s):  
Enzyme Activity ◽  
Control Diet ◽  
Normal Liver ◽  
Liver Parenchyma ◽  
Nuclease Activity ◽  
Tumor Formation ◽  
Hepatic Tissue ◽  
Similar Distribution ◽  
Acid Hydrolases ◽  
Polyadenylic Acid

Films of polyadenylic acid (poly-A) were exposed to liver sections from 4-dimethylaminoazobenzene (DAB)-fed rats in order to determine whether the nucleases acting on these films, like the ribonucleases (RNases), are depressed during carcinogenesis. Normal liver parenchyma gave a positive reaction which was particularly intense in periportal areas. Livers from animals fed the basal control diet showed a similar distribution of enzyme activity but were generally more active than normal livers. In DAB-fed rats, the nodules of hepatic tissue gave intense reactions while the trabeculae of bile ducts and connective tissue, as well as the necrotic areas, were negative. The formation of hyperbasophilic foci at later stages of DAB feeding was accompanied by a loss of enzyme activity. The hepatomas, which apparently derived from such foci, showed weak or negligible activity. Thus the changes in RNases and poly-A hydrolases occur at different stages of the carcinogenic process. The loss of RNase activity precedes the neoplastic transformation while the decrease in the activity of poly-A hydrolases is closely associated with tumor formation, but the induced tumors are deficient in both types of nuclease activity.

scholarly journals THE HISTOCHEMICAL DEMONSTRATION OF NUCLEASE ACTIVITY WITH FILMS OF SOLUBLE RIBONUCLEIC ACID AND POLYADENYLIC ACID

1972 ◽  
Vol 20 (5) ◽  
pp. 350-357 ◽  
Author(s):  
R. DAOUST ◽  
R. MORAIS
Keyword(s):  
Enzyme Activity ◽  
Ribonucleic Acid ◽  
Nuclease Activity ◽  
Histochemical Demonstration ◽  
The Other ◽  
Rat Tissues ◽  
Other Hand ◽  
Polyadenylic Acid ◽  
Normal Rat

Films of soluble ribonucleic acid (sRNA) and polyadenylic acid (poly-A) were used to investigate the distribution of nuclease activity in normal rat tissues. The reactions obtained with films of sRNA were similar to those previously observed with standard RNA and both substrates apparently reveal the same group of nucleases. On the other hand, the distribution of enzyme activity shown by films of poly-A differed markedly from that observed with RNA films, and it appears that films of poly-A demonstrate a different group of nucleases.

scholarly journals THE DISTRIBUTION OF DEOXYRIBONUCLEASE IN NORMAL, CIRRHOTIC AND NEOPLASTIC RAT LIVERS

1959 ◽  
Vol 7 (2) ◽  
pp. 139-143 ◽  
Author(s):  
R. DAOUST ◽  
A. CANTERO
Keyword(s):  
Enzyme Activity ◽  
Bile Duct ◽  
Normal Liver ◽  
Liver Parenchyma ◽  
Cirrhotic Liver ◽  
The Other ◽  
Parenchymal Cells ◽  
Rat Livers ◽  
Film Method ◽  
Dna Film

The distribution of deoxyribonuclease in normal, cirrhotic and neoplastic rat livers was investigated histochemically using the gelatine-DNA film method. The bile duct cells and connective tissue elements which are present in abnormal amounts in cirrhotic liver contain little DNAase activity compared with parenchymal tissue. The distribution pattern of DNAase is relatively uniform in normal liver parenchyma but becomes heterogeneous in the parenchyma of cirrhotic and neoplastic tissues. Groups of parenchymal cells in cirrhotic liver and the hepatoma cells in general appear devoid of DNAase activity. The necrotic areas of tissues, on the other hand, show intense enzyme activity.

CT attenuation of normal liver parenchyma on delayed scanning with iohexol

1992 ◽  
Vol 33 (5) ◽  
pp. 502-503
Author(s):  
G. Tidebrant ◽  
P. Lukes ◽  
A. Wihed
Keyword(s):  
Normal Liver ◽  
Liver Parenchyma ◽  
Normal Liver Parenchyma ◽  
Ct Attenuation

scholarly journals Impact of Pharmaceutical Prophylaxis on Radiation-Induced Liver Disease Following Radioembolization

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1992
Author(s):  
Max Seidensticker ◽  
Matthias Philipp Fabritius ◽  
Jannik Beller ◽  
Ricarda Seidensticker ◽  
Andrei Todica ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Liver Disease ◽  
Positive Impact ◽  
Metastatic Breast ◽  
Normal Liver ◽  
Liver Parenchyma ◽  
Breast Cancer Patients ◽  
Radiation Induced ◽  
The Impact

Background: Radioembolization (RE) with yttrium-90 (90Y) resin microspheres yields heterogeneous response rates in with primary or secondary liver cancer. Radiation-induced liver disease (RILD) is a potentially life-threatening complication with higher prevalence in cirrhotics or patients exposed to previous chemotherapies. Advances in RILD prevention may help increasing tolerable radiation doses to improve patient outcomes. This study aimed to evaluate the impact of post-therapeutic RILD-prophylaxis in a cohort of intensely pretreated liver metastatic breast cancer patients; Methods: Ninety-three patients with liver metastases of breast cancer received RE between 2007 and 2016. All Patients received RILD prophylaxis for 8 weeks post-RE. From January 2014, RILD prophylaxis was changed from ursodeoxycholic acid (UDCA) and prednisolone (standard prophylaxis [SP]; n = 59) to pentoxifylline (PTX), UDCA and low-dose low molecular weight heparin (LMWH) (modified prophylaxis (MP); n = 34). The primary endpoint was toxicity including symptoms of RILD; Results: Dose exposure of normal liver parenchyma was higher in the modified vs. standard prophylaxis group (47.2 Gy (17.8–86.8) vs. 40.2 Gy (12.5–83.5), p = 0.017). All grade RILD events (mild: bilirubin ≥ 21 µmol/L (but <30 μmol/L); severe: (bilirubin ≥ 30 µmol/L and ascites)) were observed more frequently in the SP group than in the MP group, albeit without significance (7/59 vs. 1/34; p = 0.140). Severe RILD occurred in the SP group only (n = 2; p > 0.1). ALBI grade increased in 16.7% patients in the MP and in 27.1% patients in the SP group, respectively (group difference not significant); Conclusions: At established dose levels, mild or severe RILD events proved rare in our cohort. RILD prophylaxis with PTX, UDCA and LMWH appears to have an independent positive impact on OS in patients with metastatic breast cancer and may reduce the frequency and severity of RILD. Results of this study as well as pathophysiological considerations warrant further investigations of RILD prophylaxis presumably targeting combinations of anticoagulation (MP) and antiinflammation (SP) to increase dose prescriptions in radioembolization.

Different Expression of Esterase Variants in Rat Hepatic and Hepatom a-Derived Cell Lines Detected by Electrophoresis

1995 ◽  
Vol 50 (9-10) ◽  
pp. 664-668 ◽  
Author(s):  
Adel S. Afify ◽  
Yoshimitsu Yamazaki ◽  
Yu-ichi Kageyama ◽  
Shiro Yusa ◽  
Yoshikatsu Ogawa ◽  
...  
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Hepatoma Cell ◽  
Polyacrylamide Gel Electrophoresis ◽  
Liver Homogenate ◽  
Normal Liver ◽  
Liver Parenchyma ◽  
Hepatoma Cell Lines ◽  
Normal Rat ◽  
Transformed Cell Lines

Abstract Esterases in nine rat hepatic and hepatoma-derived cell lines and normal rat liver homogenate were detected by polyacrylamide gel electrophoresis coupled with active staining with a-naphthyl acetate or butyrate as a substrate. The esterase band patterns of the non-cancerous and oncogene-transformed cell lines were alike, but different from those of hepatoma cell lines and normal rat liver homogenate. The former groups of cells might have completely lost the characteristics of rat liver parenchymal cells, or else they might have their origin at cells other than liver parenchyma. The esterase patterns of the hepatoma cell lines (e.g., McA-RH7777) and the normal rat liver highly resembled with each other, exemplifying the slight biochemical deviation of cancer from normal cells. However, two-dimensional electrophoretogram for the McA-RH7777 cell line showed a prominent esterase spot {p/ 6.0-Mr 110 kDa) that was lacking in the normal liver. This result indicates that there is invariably some change in esterase expression between the cancer cells and the normal liver cells

scholarly journals Histopathological and immunocytochemical changes in the liver of patients with chronic hepatitis C

2004 ◽  
Vol 61 (1) ◽  
pp. 29-34
Author(s):  
Maja Jovanovic ◽  
Ljiljana Konstantinovic ◽  
Vuka Katic ◽  
Slavisa Ciric ◽  
Velimir Kostic ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Liver Damage ◽  
Liver Parenchyma ◽  
Hepatic Tissue ◽  
Biopsy Specimens ◽  
Viral Hepatitis C ◽  
Viremia Level ◽  
Chronic Active Viral Hepatitis

Background. The presence of lymphocytes within the liver parenchyma is related to immunologically mediated liver damage in chronic hepatitis C. The aim of the study was to make histological, histochemical, and immunocytochemical assessment of liver biopsy specimens in patients with chronic hepatitis C virus (HCV) infection. Methods. Biopsy specimens of 20 patients with chronic HCV disease were analyzed, using standard staining procedures to verify histologic liver lesions, as well as immunoenzymatic staining with monoclonal antibodies to detect CD4+ T-lymphocytes, B-lymphocytes, and macrophages. Results. Micromorphologic characteristics of chronic active viral hepatitis C were present in all the patients, differing, hower, by the level of their activity. Dominant changes were found within the portal space, consisting of mononuclear lympho-plasmocytic infiltration and macrophages. Immunocytochemical investigation of mononuclear and macrophageal infiltration showed the correlation between micromorphological findings and the degree of the activity. Conclusion. The presence of lymphocytic and macrophageal infiltration within the hepatic tissue directly correlated with the intensity of the liver damage. Analysis of the population of cellular infiltrate in the liver together with the monitoring of viremia level and the level of hepatocyte necrosis, could be useful tools for elucidation of the pathogenesis of chronic hepatitis C.

Hypocholesterolemic effects of watermelon fruit rind on rats

2018 ◽  
Vol 48 (5) ◽  
pp. 836-845 ◽  
Author(s):  
Hind Abu-Hiamed
Keyword(s):  
Total Cholesterol ◽  
Normal Control ◽  
Citrullus Lanatus ◽  
Control Diet ◽  
Low Density Lipoproteins ◽  
Hepatic Tissue ◽  
Low Density ◽  
Hypocholesterolemic Effect ◽  
Content Type ◽  
Fruit Rind

Purpose Watermelon (Citrullus lanatus) fruit and its rind are known to contain phytochemicals that may have health benefits. The aim of this paper is to investigate the potential hypocholesterolemic effect of watermelon fruit rind (WR) using rats who are fed a high-cholesterol diet. Design/methodology/approach Rats were divided into six groups and fed diets for eight weeks containing normal control diet or normal control diet with either 1% cholesterol, 5% WR, 10% WR, 1% cholesterol + 5% WR or 1% cholesterol + 10% WR. Triglycerides, total cholesterol and lipoprotein levels in serum and liver samples were determined, and histopathological examination of liver tissues was carried out. Findings Diets containing 1% cholesterol led to hypercholesterolemia, characterized by increased levels of total cholesterol and low-density lipoproteins in rat serum and liver samples. Incorporation of 10% WR into the diet of the otherwise hypercholesterolemic rats led to significant reduction in serum levels of total cholesterol (from 266.2 to 222.7 mg/dL) and low-density lipoproteins (from 159.5 to 94.4 mg/dL). In addition, these rats also exhibited improvements in hepatic tissue structure compared to the hypercholesterolemic rats. Originality/value These results support the potential use of WR as a hypocholesterolemic agent. Further research is needed to ascertain the hypocholesterolemic effect of WR in human.

Effect of Repeated Normothermic Ischaemia on Normal Liver Parenchyma

1987 ◽  
Vol 28 (2) ◽  
pp. 215-219
Author(s):  
H. Stridbeck ◽  
T. Holmin ◽  
I. Hägerstrand
Keyword(s):  
Portal Vein ◽  
Hepatic Artery ◽  
Alternative Treatment ◽  
Balloon Catheter ◽  
Normal Liver ◽  
Liver Parenchyma ◽  
Histopathologic Examination ◽  
Liver Tumours ◽  
Small Areas ◽  
Minimal Damage

Hepatic ischaemia was induced in 8 mesenterico-cavally shunted pigs. The hepatic artery was occluded with biodegradable starch microspheres and the portal vein with a balloon catheter. The liver was kept ischaemic for 90 minutes at the beginning, and 90 minutes at the end, of a 24-hour period. At histopathologic examination 6 of the livers had multiple, small areas of necrosis. The total volume of these necroses accounted for no more than 10 to 15 per cent of each liver. No necrosis was evident in 2 livers. Thus normothermic Ischaemia for 2 periods of 90 minutes each within a 24-hour period caused minimal damage to the liver. An alternative treatment in patients with liver tumours could therefore be simultaneous occlusion of the hepatic artery and the portal vein.

scholarly journals PREOPERATIVE COMPUTED TOMOGRAPHY VOLUMETRY AND GRAFT WEIGHT ESTIMATION IN ADULT LIVING DONOR LIVER TRANSPLANTATION

2017 ◽  
Vol 30 (1) ◽  
pp. 38-41 ◽  
Author(s):  
Rafael S. PINHEIRO ◽  
Ruy J. CRUZ-JR ◽  
Wellington ANDRAUS ◽  
Liliana DUCATTI ◽  
Rodrigo B. MARTINO ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Living Donor ◽  
Living Donor Liver Transplantation ◽  
Normal Liver ◽  
Liver Parenchyma ◽  
Donor Liver ◽  
Donor Liver Transplantation ◽  
Graft Weight ◽  
Computed Tomography Volumetry ◽  
Graft Volume

ABSTRACT Background: Computed tomography volumetry (CTV) is a useful tool for predicting graft weights (GW) for living donor liver transplantation (LDLT). Few studies have examined the correlation between CTV and GW in normal liver parenchyma. Aim: To analyze the correlation between CTV and GW in an adult LDLT population and provide a systematic review of the existing mathematical models to calculate partial liver graft weight. Methods: Between January 2009 and January 2013, 28 consecutive donors undergoing right hepatectomy for LDLT were retrospectively reviewed. All grafts were perfused with HTK solution. Estimated graft volume was estimated by CTV and these values were compared to the actual graft weight, which was measured after liver harvesting and perfusion. Results: Median actual GW was 782.5 g, averaged 791.43±136 g and ranged from 520-1185 g. Median estimated graft volume was 927.5 ml, averaged 944.86±200.74 ml and ranged from 600-1477 ml. Linear regression of estimated graft volume and actual GW was significantly linear (GW=0.82 estimated graft volume, r2=0.98, slope=0.47, standard deviation of 0.024 and p<0.0001). Spearman Linear correlation was 0.65 with 95% CI of 0.45 - 0.99 (p<0.0001). Conclusion: The one-to-one rule did not applied in patients with normal liver parenchyma. A better estimation of graft weight could be reached by multiplying estimated graft volume by 0.82.

Effect of ischaemia on the enzyme activity of the hepatic tissue

1977 ◽  
Vol 170 (3) ◽  
pp. 259-270 ◽  
Author(s):  
P. Kupcsulik ◽  
K. Stekker ◽  
M. Németh
Keyword(s):  
Enzyme Activity ◽  
Hepatic Tissue
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