Histopathological and immunocytochemical changes in the liver of patients with chronic hepatitis C

Background. The presence of lymphocytes within the liver parenchyma is related to immunologically mediated liver damage in chronic hepatitis C. The aim of the study was to make histological, histochemical, and immunocytochemical assessment of liver biopsy specimens in patients with chronic hepatitis C virus (HCV) infection. Methods. Biopsy specimens of 20 patients with chronic HCV disease were analyzed, using standard staining procedures to verify histologic liver lesions, as well as immunoenzymatic staining with monoclonal antibodies to detect CD4+ T-lymphocytes, B-lymphocytes, and macrophages. Results. Micromorphologic characteristics of chronic active viral hepatitis C were present in all the patients, differing, hower, by the level of their activity. Dominant changes were found within the portal space, consisting of mononuclear lympho-plasmocytic infiltration and macrophages. Immunocytochemical investigation of mononuclear and macrophageal infiltration showed the correlation between micromorphological findings and the degree of the activity. Conclusion. The presence of lymphocytic and macrophageal infiltration within the hepatic tissue directly correlated with the intensity of the liver damage. Analysis of the population of cellular infiltrate in the liver together with the monitoring of viremia level and the level of hepatocyte necrosis, could be useful tools for elucidation of the pathogenesis of chronic hepatitis C.

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Modern clinical laboratory aspects of chronic hepatitis C

Experimental and Clinical Gastroenterology ◽

10.31146/1682-8658-ecg-191-7-37-42 ◽

2021 ◽

pp. 37-42

Author(s):

O. N. Sumlivaia ◽

M. S. Nevzorova ◽

A. T. Sayfitova ◽

S. A. Vysotin

Keyword(s):

Chronic Hepatitis ◽

Hepatitis C ◽

Viral Hepatitis ◽

Chronic Hepatitis C ◽

Clinical Examination ◽

Liver Damage ◽

Clinical Laboratory ◽

Diagnostic Value ◽

Control Group ◽

Viral Hepatitis C

Aim. Assess the current diagnostic value of clinical laboratory markers of liver damage in chronic viral hepatitis C. Materials and methods. Comprehensive clinical examination of 194 patients with the diagnosis chronic hepatitis C and 73 almost healthy faces was conducted. Results. According to the clinical examination, 91% of patients have hepatomegaly. According to the results of elastography, the subgroup without fibrosis F0 49 (25%) people, with fibrosis of stages F1-F3-145 (75%) patients. The occurrence of HCV genotypes was: HCV-1-33%, HCV-2-12% and HCV-3-55%. The virusemia indicator showed large variations of values. During the research it was established that indirect indicators of a fibrosis of a liver: levels of AST and ALT, GGTP, a direct and general bilirubin, the APRI index are authentically exceeded by indexes of control group while the level of thrombocytes and de Ritis’s coefficient authentically decrease. Conclusions. The current course of chronic hepatitis C occurs with hepatomegalia, often associated with gastrointestinal and cardiovascular damage. The most common virus among patients is the HVC-3 genotype virus. When examining indirect markers of fibrosis in viral hepatitis C, the APRI test is an early predictor and has high predictive value. Platelet levels decrease with viral liver damage. As a consequence, the APRI index increases and the de Ritis ratio decreases.

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P.128 LIVER EXPRESSION OF RBP4 CORRELATES WITH LIVER DAMAGE IN CHRONIC HEPATITIS C AND IN NONALCOHOLIC FATTY LIVER DISEASE

Digestive and Liver Disease ◽

10.1016/s1590-8658(10)60241-1 ◽

2010 ◽

Vol 42 ◽

pp. S148

Author(s):

S. Petta ◽

C. Tripodo ◽

S. Grimaudo ◽

C. Cammà ◽

D. Cabibi ◽

...

Keyword(s):

Chronic Hepatitis ◽

Liver Disease ◽

Hepatitis C ◽

Fatty Liver ◽

Nonalcoholic Fatty Liver Disease ◽

Chronic Hepatitis C ◽

Liver Damage ◽

Fatty Liver Disease ◽

Nonalcoholic Fatty Liver

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Significance of iron reduction for the therapy of chronic hepatitis C

Vojnosanitetski pregled ◽

10.2298/vsp0502161n ◽

2005 ◽

Vol 62 (2) ◽

pp. 161-164 ◽

Cited By ~ 1

Author(s):

Darko Nozic ◽

Bela Balint ◽

Nebojsa Stankovic ◽

Jovan Dimitrijevic ◽

Gorana Neskovic

Keyword(s):

Chronic Hepatitis ◽

Hepatitis C ◽

Iron Overload ◽

Antiviral Therapy ◽

Chronic Hepatitis C ◽

Iron Reduction ◽

Elevated Serum ◽

Chronic Viral Hepatitis ◽

Viral Hepatitis C ◽

Iron Deposits

Background. It has been established that many patients with chronic hepatitis C have elevated serum iron, feritin levels and iron deposits in the liver. Therefore, the liver damage due to hepatitis C virus may be aggravated with iron overload. In many studies higher levels of iron in the blood and the liver were connected with the decreased response to interferon-alfa therapy for chronic viral hepatitis C. Recent introduction of pegylated interferons plus ribavirin has improved the therapeutic response, so it is now possible to cure more than 50% of the patients. Case report. Three patients with chronic hepatitis C and iron overload were presented. Iron reduction therapy using phlebotomy or eritrocytapheresis with plasmapheresis was done at different times in regard to specific antiviral therapy or as a sole therapy. Conclusion. It has been shown that iron reduction, sole or combined with antiviral therapy, led to the deacreased aminotransferase serum activity and might have slow down the evolution of chronic hepatitis C viral infection.

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The association gene’s TNFα G(308) with quantity of TNFα and degree of liver’s fibrosis in patients with chronic hepatitis C

Reports of Vinnytsia National Medical University ◽

10.31393/reports-vnmedical-2018-22(4)-19 ◽

2018 ◽

Vol 22 (4) ◽

pp. 682-685

Author(s):

E.N. Usychenko ◽

Yu.I. Bazhora ◽

E.M. Usychenko ◽

V.A. Gudz

Keyword(s):

Chronic Hepatitis ◽

Hepatitis C ◽

Liver Fibrosis ◽

Chronic Hepatitis C ◽

Degrees Of Freedom ◽

Morphological Changes ◽

Polymorphic Marker ◽

Pathological Process ◽

Hepatic Tissue ◽

The Individual

The data on the polymorphism of cytokine genes associated with individual reactivity on the effects of hepatitis C virus, predict the rate of progression of liver fibrosis. The purpose of this work is study the association of the polymorphic marker G308A of the TNFα gene with its quantitative content and degree of liver fibrosis in patients with chronic hepatitis C. A total of 100 patients with CSF were examined. The polymorphism of G308A gene’s TNFα was studied by amplification of the corresponding genome zones by PCR. The assessment of the degree of fibrosis was performed using the non-invasive Fibrotest method. The study of the quantity of TNFα cytokine in serum of patients was performed by ELISA. The distribution of genotypes on the investigated polymorphic loci was verified using Pearson's χ2 criterion. The frequencies of alleles and genotypes in the groups were compared using Pearson's χ2 criterion with Yates correction for continuity with the number of degrees of freedom 1. In order to detect the correlation dependencies between the individual parameters, the Spearman correlation coefficient was applied. It was found that a smaller degree of fibrosis was observed in carriers of the GG TNFα genotype, and a greater degree of fibrosis in the carriers of the genotype AA TNFα (moderate feedback between the degree of fibrosis and the genotypes of TNFα). The higher content of TNFα is noted in the carriers of the AA genotype TNFα, the lower content of TNFα - in the carriers of the GG TNFα genotype (moderate feedback between the TNFα genotypes and the TNFα content). It has been established that a higher TNFα content is observed in patients with F1-F0 fibrosis, a lower TNFα content in patients with F2-F3 fibrosis (a strong correlation between the degree of fibrosis and the amount of TNFα cytokine). It is assumed that the production of the cytokine is determined at the genetic level, and the severity of changes in the cytokine profile in chronic hepatitis C affects the course of the pathological process. An increase in the TNFα content in chronic hepatitis C may be a marker for significant morphological changes in the hepatic tissue and high activity of the inflammatory process.

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Patatin-Like phospholipase domain-containing 3 I148M polymorphism, steatosis, and liver damage in chronic hepatitis C

Hepatology ◽

10.1002/hep.24123 ◽

2011 ◽

Vol 53 (3) ◽

pp. 791-799 ◽

Cited By ~ 182

Author(s):

Luca Valenti ◽

MariaGrazia Rumi ◽

Enrico Galmozzi ◽

Alessio Aghemo ◽

Benedetta Del Menico ◽

...

Keyword(s):

Chronic Hepatitis ◽

Hepatitis C ◽

Chronic Hepatitis C ◽

Liver Damage

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Fibrosis in Chronic Hepatitis C: Correlation between Immunohistochemically-Assessed Virus Load with Steatosis and Cellular Iron Content

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2016.122 ◽

2016 ◽

Vol 4 (4) ◽

pp. 578-584

Author(s):

Maha Akl ◽

Ali EL Hindawi ◽

Maha Mosaad ◽

Ahmed Montasser ◽

Ahmed El Ray ◽

...

Keyword(s):

Chronic Hepatitis ◽

Viral Load ◽

Hepatitis C ◽

Chronic Hepatitis C ◽

Liver Damage ◽

Cellular Iron ◽

Iron Load ◽

Cirrhotic Group ◽

Sirius Red

AIM: We aimed study impact of hepatocytic viral load, steatosis, and iron load on fibrosis in chronic hepatitis C and role of VEGF and VEGFR overexpression in cirrhotic cases in evolving HCC.MATERIAL AND METHODS: Total of 120 cases were included from TBRI and Beaujon Hospital as chronic hepatitis C (CHC), post-hepatitis C cirrhosis, and HCC. Cases of CHC were stained for Sirius red, Prussian blue and immunohistochemically (IHC) for HCV-NS3/NS4. HCC were stained IHC for VEGF and by FISH.RESULTS: Stage of fibrosis was significantly correlated with inflammation in CHC (P < 0.01). Noticed iron load did not correlate with fibrosis. Steatosis was associated with higher inflammation and fibrosis. The cellular viral load did not correlate with inflammation, steatosis or fibrosis. VEGF by IHC was significantly higher in cases of HCC when compared to cirrhotic group (P < 0.001). Amplification of VEGFR2 was confirmed in 40% of cases of HCC. Scoring of VEGF by IHC was the good indicator of VEGFR2 amplification by FISH (P < 0.005).CONCLUSION: Grade of inflammation is the factor affecting fibrosis in CHC. The degree of liver damage is not related to cellular viral load or iron load. Steatosis is associated with higher inflammation and fibrosis. VEGF by IHC is correlated with overexpression of VEGFR2 by FISH.

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