Development of a tissue-engineered skin substitute on a base of human amniotic membrane

Allogenic graft material and tissue engineering have recently shown promising results for the improvement of both esthetic and functional outcomes in the treatment of large skin defects. We chose human amniotic membrane as a cellular scaffold in order to develop a skin substitute for later in vivo uses. Various methods of de-epithelialization of the human amniotic membrane were evaluated by histological analysis including hematoxylin–eosin and laminin staining, optic coherence tomography, and scanning electron microscopy with 0.25/0.02% trypsin/ethylenediaminetetraacetic acid treatment and mechanical cell removal showing an almost complete loss of the epithelium and a mainly intact basement membrane. Novel examination of human amniotic membrane by optic coherence tomography was feasible, but difficulties were experienced in handling and interpretation of the tissue as no comparable data exist. Subsequently, we developed an air–liquid interface cell culture to cultivate keratinocytes and fibroblasts on the de-epithelialized human amniotic membrane. We achieved a mostly keratinized surface on the epidermal side with a confluent fibroblast network on the chorion side.

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Preparation and clinical application possibilities of human amniotic membrane grafts

Postępy Higieny i Medycyny Doświadczalnej ◽

10.5604/01.3001.0013.6286 ◽

2019 ◽

Vol 73 ◽

pp. 674-680

Author(s):

Agnieszka Klama-Baryła ◽

Wojciech Smętek ◽

Wojciech Łabuś ◽

Diana Kitala

Keyword(s):

Amniotic Membrane ◽

Cell Activity ◽

Tissue Bank ◽

Skin Substitute ◽

Fetal Membranes ◽

Tissue Banks ◽

Human Amniotic Membrane ◽

Graft Material ◽

Alternative Source ◽

Human Amnion

In the majority of obstetric and gynaecological wards, the fetal membranes along with the rest of the placenta are usually discarded as medical waste. However, donating placenta to the Tissue Bank may provide a highly valuable source of graft material. One of the most common tissues used in clinical transplantation is amniotic membrane. The unique properties of amnion make it highly beneficial not only in preventing bacterial infections, protein loss, fluids and electrolytes disturbances, but also in reducing burn wound pain and accelerating healing processes. Due to its abundance of nutrients and low immunogenicity, it is often used as a skin substitute. Human amnion may be prepared in tissue banks, as biostatic or biovital grafts. In order to increase surgical treatment efficiency and to prevent the risk of potential transmission of infectious diseases, a number of methods of amniotic graft preparation have been developed. This paper presents the current clinical possibilities of applying human amnion and other types of placenta tissue grafts in the treatment of wounds of various etiology. It also describes new application areas of fetal membranes for other diseases. Placental grafts are extensively used as an alternative therapy in situations when standard treatment does not produce desirable outcomes. The properties of placental grafts including i.a. low cost, easy manipulation, capability of promoting cell migration and proliferation and stimulation of stem cell activity, making them highly beneficial in comparison to many other bioactive therapies. The alternative source of graft preparation may also be harvested from animal placenta, which makes it an alternative source of supply to human tissues.

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Human Amniotic Membrane Preparation, Preservation and Clinical Application Using Various Techniques for the Treatment of Ophthalmic Dysfunctions

10.20944/preprints201810.0321.v1 ◽

2018 ◽

Author(s):

Liakat Hossain ◽

Md. Arifuzzaman ◽

Farzana Diba ◽

Ayesha Siddika ◽

MH Adnan ◽

...

Keyword(s):

Clinical Application ◽

Amniotic Membrane ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Interleukin 1 ◽

Interleukin 10 ◽

Human Amniotic Membrane ◽

Graft Material ◽

Revolutionary Change ◽

Ocular Disorders

The aim of this study is to emphasize on the clinical uses of amniotic membrane (AM) in multiple ocular disorders. AM possesses many properties including promotion of epithelialization, anti-fibrotic, anti-apoptotic, anti-angiogenic properties. Epithelial wound healing on the eye surface is promoted due to several epidermal and keratocyte growth factors as well as by its anti-inflammatory and anti-scarring effects due to interleukin-10, interleukin-1 receptor antagonists and inhibition of transforming growth factor- beta (TGF-β) signal transduction by AM in the corneal and conjunctival fibroblasts respectively. It plays a crucial role by functioning as a substitute of basement membrane (BM) and as a temporary graft material. It is not only being used as an eye graft but also its extract can be used as an eye drop for corneal and external eye diseases. So its proper preparation, preservation and clinical application can bring a revolutionary change in the treatment of different ocular disorders.

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Human amniotic membrane extracellular matrix scaffold for dental pulp regeneration in vitro and in vivo

International Endodontic Journal ◽

10.1111/iej.13675 ◽

2021 ◽

Author(s):

Hengameh Bakhtiar ◽

Azin Ashoori ◽

Sarah Rajabi ◽

Mohamad Pezeshki‐Modaress ◽

Alireza Ayati ◽

...

Keyword(s):

Extracellular Matrix ◽

Amniotic Membrane ◽

Dental Pulp ◽

Human Amniotic Membrane ◽

Pulp Regeneration ◽

Regeneration In Vitro ◽

Extracellular Matrix Scaffold

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Tissue-Engineered Skin Substitutes Using Collagen Scaffold with Amniotic Membrane Component

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.277-279.12 ◽

2005 ◽

Vol 277-279 ◽

pp. 12-18 ◽

Cited By ~ 2

Author(s):

Eun Kyung Yang ◽

Jung Keug Park ◽

Jae Il Ahn ◽

Hee Gu Lee ◽

Seong Jun Seo ◽

...

Keyword(s):

Wound Healing ◽

Amniotic Membrane ◽

Wound Repair ◽

Collagen Scaffold ◽

Animal Origin ◽

Skin Substitute ◽

Skin Substitutes ◽

Membrane Component ◽

Early Degradation

Human skin substitutes are needed for implantation and wound repair based on the new concept of tissue engineering in combination with biomaterials and cell biological technology. However, failure sometimes occurs when the wound healing is delayed in vivo due to acute inflammation resulting from the early degradation of the transplanted biomaterials. Accordingly, the current study modified conventional biomaterials to overcome early degradation and strong inflammation. In a conventional skin substitute, the animal origin collagenous materials have a slight antigenicity as xenogenic materials, however, the modified method was able to obtain a low antigenicity and anti-inflammation effect using atelo-collagen and an amniotic component. The tyrosine content in the developed atelo-collagen, representing the antigenicity, was reduced from 0.590% to 0.046% based on an HPLC analysis. In addition, to reduce the inflammation and foreign material reaction, an amniotic component was applied to the atelo-collagen materials. While, to enhance the wound healing, the modified skin substitute was developed as a composite matrix of an atelo-collagen scaffold with an amniotic membrane component. A quantitative analysis of hEGF in the amniotic membrane was also performed using different processing methods. Finally, a tissueengineered skin substitute was constructed by cultivating skin cells in the collagen scaffold attached to an amniotic membrane.

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In vivo confocal microscopy of human cornea covered with human amniotic membrane

Japanese Journal of Ophthalmology ◽

10.1007/s10384-008-0578-y ◽

2008 ◽

Vol 52 (6) ◽

pp. 493-496 ◽

Cited By ~ 4

Author(s):

Tatsuya Mimura ◽

Satoru Yamagami ◽

Tomohiko Usui ◽

Norihiko Honda ◽

Fumiyuki Araki ◽

...

Keyword(s):

Confocal Microscopy ◽

Amniotic Membrane ◽

Human Amniotic Membrane ◽

Human Cornea ◽

In Vivo Confocal Microscopy

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A skin substitute based on human amniotic membrane

Cell and Tissue Banking ◽

10.1007/s10561-014-9427-z ◽

2014 ◽

Vol 15 (2) ◽

pp. 257-265 ◽

Cited By ~ 12

Author(s):

Hélène Tauzin ◽

Gwenaël Rolin ◽

Céline Viennet ◽

Philippe Saas ◽

Philippe Humbert ◽

...

Keyword(s):

Amniotic Membrane ◽

Skin Substitute ◽

Human Amniotic Membrane

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Human amniotic membrane derived-mesenchymal stem cells induce C6 glioma apoptosis in vivo through the Bcl-2/caspase pathways

Molecular Biology Reports ◽

10.1007/s11033-011-0760-z ◽

2011 ◽

Vol 39 (1) ◽

pp. 467-473 ◽

Cited By ~ 26

Author(s):

Hongliang Jiao ◽

Fangxia Guan ◽

Bo Yang ◽

Jianbin Li ◽

Laijun Song ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Amniotic Membrane ◽

C6 Glioma ◽

Human Amniotic Membrane

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Human Amniotic Membrane Preparation, Preservation and Clinical Application Using Various Techniques for the Treatment of Ophthalmic Dysfunctions

10.20944/preprints201810.0307.v1 ◽

2018 ◽

Author(s):

Md. Arifuzzaman ◽

Md. Liakat Hossain ◽

Farzana Diba ◽

Ayesha Siddika ◽

MH Adnan ◽

...

Keyword(s):

Clinical Application ◽

Amniotic Membrane ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Interleukin 1 ◽

Interleukin 10 ◽

Human Amniotic Membrane ◽

Graft Material ◽

Revolutionary Change ◽

Ocular Disorders

Abstract: The aim of this study is to emphasize on the clinical uses of amniotic membrane (AM) in multiple ocular disorders. AM possesses many properties including promotion of epithelialization, anti-fibrotic, anti-apoptotic, anti-angiogenic properties. Epithelial wound healing on the eye surface is promoted due to several epidermal and keratocyte growth factors as well as by its anti-inflammatory and anti-scarring effects due to interleukin-10, interleukin-1 receptor antagonists and inhibition of transforming growth factor- beta (TGF-β) signal transduction by AM in the corneal and conjunctival fibroblasts respectively. It plays a crucial role by functioning as a substitute of basement membrane (BM) and as a temporary graft material. It is not only being used as an eye graft but also its extract can be used as an eye drop for corneal and external eye diseases. So its proper preparation, preservation and clinical application can bring a revolutionary change in the treatment of different ocular disorders.

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Human Amniotic Membrane and Amniotic Membrane–Derived Cells

Cell Transplantation ◽

10.1177/0963689717725528 ◽

2018 ◽

Vol 27 (1) ◽

pp. 77-92 ◽

Cited By ~ 13

Author(s):

Taja Železnik Ramuta ◽

Mateja Erdani Kreft

Keyword(s):

Amniotic Membrane ◽

Antimicrobial Properties ◽

Organ Damage ◽

Human Amniotic Membrane ◽

Loss Of Function ◽

Amniotic Cavity ◽

Reconstructive Urology ◽

And Storage

Human amniotic membrane (hAM) is the innermost layer of fetal membranes, which surrounds the developing fetus and forms the amniotic cavity. hAM and hAM-derived cells possess many properties that make them suitable for use in regenerative medicine, such as low immunogenicity, promotion of epithelization, anti-inflammatory properties, angiogenic and antiangiogenic properties, antifibrotic properties, antimicrobial properties, and anticancer properties. Many pathological conditions of the urinary tract lead to organ damage or complete loss of function. Consequently, the reconstruction or replacement of damaged organs is needed, which makes searching for new approaches in regenerative and reconstructive urology a necessity. The use of hAM for treating defects in kidneys, ureters, urinary bladder, and urethra was tested in vitro in cell cultures and in vivo in mice, rats, rabbits, cats, dogs, and also in humans. These studies confirmed the advantages and the potential of hAM for use in regenerative and reconstructive urology as stated above. However, they also pointed out a few concerns we have to take into consideration. These are (1) the lack of a standardized protocol in hAM preparation and storage, (2) the heterogeneity of hAM, and especially (3) low mechanical strength of hAM. Before any wider use of hAM for treating urological defects, the protocols for preparation and storage will need to be standardized, followed by more studies on larger animals and clinical trials, which will altogether extensively assess the potential of hAM use in urological patients.

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