Detecting and Trapping of a Single C. elegans Worm in a Microfluidic Chip for Automated Microplate Dispensing

Microfluidic devices offer new technical possibilities for a precise manipulation of Caenorhabditis elegans due to the comparable length scale. C. elegans is a small, free-living nematode worm that is a popular model system for genetic, genomic, and high-throughput experimental studies of animal development and neurobiology. In this paper, we demonstrate a microfluidic system in polydimethylsiloxane (PDMS) for dispensing of a single C. elegans worm into a 96-well plate. It consists of two PDMS layers, a flow and a control layer. Using five microfluidic pneumatic valves in the control layer, a single worm is trapped upon optical detection with a pair of optical fibers integrated perpendicular to the constriction channel and then dispensed into a microplate well with a dispensing tip attached to a robotic handling system. Due to its simple design and facile fabrication, we expect that our microfluidic chip can be expanded to a multiplexed dispensation system of C. elegans worms for high-throughput drug screening.

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Biophysical phenotyping of C. elegans in a microfluidic chip for high-throughput drug screening

10.1016/b978-0-12-823990-2.00012-x ◽

2021 ◽

pp. 261-293

Author(s):

Samuel Sofela ◽

Yongxiang Feng ◽

Navajit S. Baban ◽

Christopher J. Stubbs ◽

Yong-Ak Song ◽

...

Keyword(s):

High Throughput ◽

Drug Screening ◽

Microfluidic Chip ◽

C Elegans ◽

High Throughput Drug Screening

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Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1609569114 ◽

2016 ◽

Vol 113 (52) ◽

pp. 14915-14920 ◽

Cited By ~ 21

Author(s):

Yih Yang Chen ◽

Pamuditha N. Silva ◽

Abdullah Muhammad Syed ◽

Shrey Sindhwani ◽

Jonathan V. Rocheleau ◽

...

Keyword(s):

High Throughput ◽

High Throughput Screening ◽

Three Dimensional ◽

Microfluidic System ◽

High Throughput Drug Screening ◽

Image Analysis Algorithm ◽

Screening Assays ◽

On Chip ◽

Tissue Models

On-chip imaging of intact three-dimensional tissues within microfluidic devices is fundamentally hindered by intratissue optical scattering, which impedes their use as tissue models for high-throughput screening assays. Here, we engineered a microfluidic system that preserves and converts tissues into optically transparent structures in less than 1 d, which is 20× faster than current passive clearing approaches. Accelerated clearing was achieved because the microfluidic system enhanced the exchange of interstitial fluids by 567-fold, which increased the rate of removal of optically scattering lipid molecules from the cross-linked tissue. Our enhanced clearing process allowed us to fluorescently image and map the segregation and compartmentalization of different cells during the formation of tumor spheroids, and to track the degradation of vasculature over time within extracted murine pancreatic islets in static culture, which may have implications on the efficacy of beta-cell transplantation treatments for type 1 diabetes. We further developed an image analysis algorithm that automates the analysis of the vasculature connectivity, volume, and cellular spatial distribution of the intact tissue. Our technique allows whole tissue analysis in microfluidic systems, and has implications in the development of organ-on-a-chip systems, high-throughput drug screening devices, and in regenerative medicine.

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Microfluidic System Based High Throughput Drug Screening System for Curcumin/TRAIL Combinational Chemotherapy in Human Prostate Cancer PC3 Cells

Biomolecules & Therapeutics ◽

10.4062/biomolther.2014.078 ◽

2014 ◽

Vol 22 (4) ◽

pp. 355-362 ◽

Cited By ~ 38

Author(s):

Dami An ◽

Kwangmi Kim ◽

Jeongyun Kim

Keyword(s):

Prostate Cancer ◽

High Throughput ◽

Drug Screening ◽

Microfluidic System ◽

Human Prostate ◽

Screening System ◽

Human Prostate Cancer ◽

High Throughput Drug Screening ◽

Pc3 Cells

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The Developing Status of High-Throughput Drug Screening Microfluidic Chip by FRET on Medicine

Materials Science Forum ◽

10.4028/www.scientific.net/msf.914.19 ◽

2018 ◽

Vol 914 ◽

pp. 19-28

Author(s):

Xin Yu Zhang ◽

Qiu Hong Huang ◽

Mei Yang ◽

Xiao Ling Liao ◽

Ze Yu Shao ◽

...

Keyword(s):

High Throughput ◽

Drug Screening ◽

Microfluidic Chip ◽

High Throughput Screening ◽

Functional Integration ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

Cell Detection ◽

High Throughput Drug Screening ◽

Efficient Detection

High-throughput drug screening microfluidic chip has good biocompatibility and faveriable functional integration, which is the excellent platform for high-throughput screening. Importantly, FRET (Fluorescence Resonance Energy Transfer) technology is the most efficient detection means at present. In this paper, we introduce the development of drug screening microfluidic chip on cellular level and the application of FRET technology on cell detection. Further, we discusse the possibility of FRET applied in the field of microfluidic biochip.

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