The Anatomy of a Nonfaradaic Electrochemical Biosensor

2017 ◽  
Vol 23 (1) ◽  
pp. 5-15 ◽  
Author(s):  
Hunter Stevenson ◽  
Nandhinee Radha Shanmugam ◽  
Anjan Paneer Selvam ◽  
Shalini Prasad
Keyword(s):  
Mathematical Modeling ◽  
Clinical Management ◽  
Electrochemical Biosensor ◽  
Point Of Care ◽  
Electrochemical Measurements ◽  
Sample Data ◽  
Trained Personnel ◽  
Analyze Sample ◽  
Model Protein ◽  
Quantitative Results

Point-of-care (POC) testing has revolutionized diagnostic healthcare, bringing medical results directly and immediately to the patient. With faster diagnostics, more immediate clinical management decisions can be made. POC tests most often use a dipstick or swab format to detect the presence of a pathogen, disease, or other relevant biomarker. In these formats, the POC tests eliminate the need for complex lab equipment and trained personnel to collect, process, and analyze sample data for simple diagnostics. However, these tests cannot satisfy all clinical needs, because accurate quantitative results are needed. The present study serves as a template for designing a nonfaradaic electrochemical biosensor toward quantitative POC diagnostics. We focus on investigating the most important parameters when constructing a nonfaradaic biosensor through both mathematical modeling and electrochemical measurements. Furthermore, we demonstrate quantitative affinity biosensing of a model protein toward developing a POC device.

The effect of Vitamin C for point-of-care blood analysis applications using an electrochemical biosensor

2013 ◽  
Author(s):  
Hadar Ben-Yoav ◽  
Sheryl E. Chocron ◽  
Thomas E. Winkler ◽  
Eunkyoung Kim ◽  
Gregory F. Payne ◽  
...  
Keyword(s):  
Vitamin C ◽  
Electrochemical Biosensor ◽  
Point Of Care ◽  
Blood Analysis

Performance Evaluation and Multicenter Consistency Study of Smartphone-based Hipee S2 Point-of-care Testing Urine Dipstick Analyser: Evaluation Study (Preprint)

2021 ◽  
Author(s):  
Qiang Zhang ◽  
Guoqing Wang ◽  
Xiaolong Zong ◽  
Jinghua Sun
Keyword(s):  
Health Management ◽  
Clinical Performance ◽  
Point Of Care ◽  
Method Comparison ◽  
Evaluation Study ◽  
Analytical Performance ◽  
Point Of Care Testing ◽  
Urine Dipstick ◽  
Quantitative Results ◽  
Carry Over

BACKGROUND With advances in mobile technology, smartphone-based point-of-care testing (POCT) urinalysis hold great potential for disease screening and health management for clinicians and individual users. The POCT devices need to have good analytical and clinical performance, but data are lacking. OBJECTIVE The purpose of this study is to evaluate the analytical performance of smartphone-based Hipee S2 POCT urine dipstick analyser. METHODS A total of 1,603 urine samples from three hospitals were collected. Precision, drift, carry-over, interference, and method comparison of Hipee S2 were evaluated. RESULTS The precision for each parameter, assessed by control materials, was acceptable. No sample carry-over or drift was observed. Ascorbate solution with 1 g/L had an inhibitory effect for the haemoglobin test. Agreement for specific gravity (SG) varied between moderate to substantial, for pondus hydrogenii (pH) was moderate, and for other parameters varied between substantial to excellent, on comparing the results obtained by Hipee S2 with those measured by laboratory reference analysers. The semi-quantitative results of microalbumin and creatinine were highly correlated with the quantitative results. CONCLUSIONS Hipee S2 POCT urine analyser showed acceptable analytical performance as a semi-quantitative method. It serves as a convenient alternate device for clinicians and individual users for urinalysis and health management.

scholarly journals Application in Europe of a urine-based rapid diagnostic test for confirmation of Schistosoma mansoni infection in migrants from endemic areas

2015 ◽  
Vol 20 (23) ◽  
Author(s):  
S L Becker ◽  
H Marti ◽  
S Zimmermann ◽  
D Vidacek ◽  
M Herrmann ◽  
...  
Keyword(s):  
Abdominal Pain ◽  
Schistosoma Mansoni ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Rectal Bleeding ◽  
Clinical Management ◽  
Point Of Care ◽  
Rectal Biopsy ◽  
Non Invasive ◽  
Persistent Abdominal Pain

In February 2015, a male patient from Eritrea with persistent abdominal pain and rectal bleeding was diagnosed with Schistosoma mansoni infection upon examination of a rectal biopsy. In May 2015, repeated stool microscopy identified S. mansoni infection in another Eritrean patient with abdominal pain and considerable eosinophilia (34%). Use of point-of-care circulating cathodic antigen (POC-CCA) tests on urine confirmed S. mansoni infection in both patients. Wider application of non-invasive POC-CCA urine tests will improve schistosomiasis diagnosis and clinical management in migrants.

scholarly journals A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material

2021 ◽  
Author(s):  
Jennifer N. Rauch ◽  
Eric Valois ◽  
Sabrina C. Solley ◽  
Friederike Braig ◽  
Ryan S. Lach ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Genetic Material ◽  
Detection Sensitivity ◽  
Biochemical Methods ◽  
Trained Personnel ◽  
Interpretation Of Results

The COVID-19 pandemic has created massive demand for widespread, distributed tools for detecting SARS-CoV-2 genetic material. The hurdles to scalable testing include reagent and instrument accessibility, availability of highly trained personnel, and large upfront investment. Here we showcase an orthogonal pipeline we call CREST (Cas13-based, Rugged, Equitable, Scalable Testing) that addresses some of these hurdles. Specifically, CREST pairs commonplace and reliable biochemical methods (PCR) with low-cost instrumentation, without sacrificing detection sensitivity. By taking advantage of simple fluorescence visualizers, CREST allows for a binary interpretation of results. CREST may provide a point-of-care solution to increase the distribution of COVID-19 surveillance.

scholarly journals Clinical impact of the rapid molecular detection of RSV and influenza A and B virus in the emergency room

2021 ◽  
Author(s):  
Nicolas Yin ◽  
Marc Van Nuffelen ◽  
Magali Bartiaux ◽  
Thierry Preseau ◽  
Inge Roggen ◽  
...  
Keyword(s):  
Emergency Room ◽  
Clinical Management ◽  
Diagnostic Tests ◽  
Influenza A ◽  
Point Of Care ◽  
Antibiotic Use ◽  
Rapid Diagnostic Tests ◽  
Analytical Performance ◽  
Molecular Test ◽  
The Impact

Introduction: Using respiratory viruses' rapid diagnostic tests in the emergency room (ER) could allow a better and faster clinical management. Point-of-care PCR instruments provide now results in less than 30 minutes. The objective of this study was to assess the impact of the use of the cobas® Influenza A/B & RSV Assay for use on Roche's cobas® Liat® instrument on the clinical management of ER patients. Methods: Patients (adults and children) requiring admission or suffering from an underlying condition at risk of respiratory complication were prospectively recruited in the ER of four hospitals in the Brussels region. Physician's intentions regarding admission, isolation, antibiotic and antiviral use were collected before and after performing a cobas® Influenza A/B & RSV molecular test. Additionally, a comparison of the analytical performance of this test against antigen rapid tests and viral culture was performed as well as a time-to-result evaluation. Results: PCR yielded twice more positive results than antigen rapid diagnostic tests. It allowed a decrease in the overall need for isolation and treatment by limiting the isolation of negative patients and the antibiotic use for positive patients. Meanwhile, antiviral treatments better targeted patients with a positive influenza PCR. Conclusion: The use of a rapid influenza and RSV molecular test improves the clinical management of patients admitted to the ER by providing a fast and reliable result. Their additional cost compared to antigen tests should be balanced with the benefit of their analytical performance, leading to efficient reductions in the need of isolation and antibiotic use.

Ultrasound

2019 ◽  
pp. 1001-1028
Author(s):  
Matthew Lyon ◽  
W. Ted Kuhn
Keyword(s):  
Clinical Management ◽  
Point Of Care ◽  
Image Acquisition ◽  
Image Interpretation ◽  
Ultrasound Image ◽  
Point Of Care Ultrasound ◽  
Clinical Integration ◽  
Humanitarian Emergency ◽  
Emergency Situations ◽  
Essential Components

This chapter outlines how clinical management decisions in humanitarian emergency situations can be greatly enhanced by the utilization of point-of-care ultrasound. The chapter recognizes the advancing technology of point-of care ultrasound and its use in humanitarian missions, and provides guidance on the essential components of clinical ultrasound (image acquisition, image interpretation, and clinical integration).

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