scholarly journals An immunohistochemical study performed with monoclonal and polyclonal antibodies to mouse epidermal growth factor.

1988 ◽  
Vol 36 (9) ◽  
pp. 1153-1160 ◽  
Author(s):  
H J Beerstecher ◽  
C Huiskens-van der Meij ◽  
S O Warnaar
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Immunohistochemical Study ◽  
Polyclonal Antibodies ◽  
Rabbit Antiserum ◽  
Kidney Tissue ◽  
Human Kidney ◽  
Immunohistochemical Analysis ◽  
Rodent Brain ◽  
Epidermal Growth

We developed two monoclonal antibodies, E5 and F5, which react with mouse epidermal growth factor (mEGF) and urogastrone. These antibodies and a rabbit antiserum to mEGF were used for immunohistochemical analysis of staining reactions in rodent and human tissues. Our results do not confirm the published reports of EGF-like immunoreactivity in Brunner's glands, human submandibular glands, human kidney tissue, or rodent brain sections.

scholarly journals IMMUNOHISTOCHEMICAL STUDY OF THE EXPRESSION OF EPIDERMAL GROWTH FACTOR (EGF) IN HUMAN GASTRIC CARCINOMAS

1993 ◽  
Vol 54 (9) ◽  
pp. 2224-2228
Author(s):  
Keishiro AOYAGI ◽  
Ken HASHIMOTO ◽  
Kikuo KOHFUJI ◽  
Issei KODAMA ◽  
Masafumi MARUIWA ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Immunohistochemical Study ◽  
Gastric Carcinomas ◽  
Epidermal Growth

scholarly journals Laminin γ3 Chain Binds to Nidogen and Is Located in Murine Basement Membranes

2005 ◽  
Vol 280 (23) ◽  
pp. 22146-22153 ◽  
Author(s):  
Nikolaus Gersdorff ◽  
Eddie Kohfeldt ◽  
Takako Sasaki ◽  
Rupert Timpl ◽  
Nicolai Miosge
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Self Assembly ◽  
Mammalian Cells ◽  
Polyclonal Antibodies ◽  
Basement Membranes ◽  
Electron Microscopic ◽  
Immunogold Staining ◽  
Adult Mice ◽  
Epidermal Growth

Recently a novel laminin γ3 chain was identified in mouse and human and shown to have the same modular structure as the laminin γ1 chain. We expressed two fragments of the γ3 chain in mammalian cells recombinantly. The first, domain VI/V, consisting of laminin N-terminal (domain VI) and four laminin-type epidermal growth factor-like (domain V) and laminin N-terminal modules, was shown to be essential for self-assembly of laminins. The other was domain III3–5, which consists of three laminin-type epidermal growth factor-like modules and is predicted to bind to nidogens. The γ3 VI/V fragment was a poor inhibitor for laminin-1 polymerization as was the β2 VI/V fragment. The γ3 III3–5 fragment bound to nidogen-1 and nidogen-2 with lower affinity than the γ1 III3–5 fragment. These data suggested that laminins containing the γ3 chain may assemble networks independent of other laminins. Polyclonal antibodies raised against γ3 VI/V and γ3 III3–5 showed no cross-reaction with homologous fragments from the γ1 and γ2 chains of laminin and allowed the establishment of γ chain-specific radioimmunoassays and light and electron microscopic immunostaining of tissues. This demonstrated a 20–100-fold lower content of the γ3 chain compared with the γ1 chain in various tissue extracts of adult mice. The expression of γ3 chain was highly tissue-specific. In contrast to earlier assumptions, the antibodies against the γ3 chain showed light microscopic staining exclusively in basement membrane zones of adult and embryonic tissues, such as the brain, kidney, skin, muscle, and testis. Ultrastructural immunogold staining localized the γ3 chain to basement membranes of these tissues.

Involvement of epidermal growth factor in inducing obesity in ovariectomized mice

1993 ◽  
Vol 265 (2) ◽  
pp. E323-E331 ◽  
Author(s):  
H. Kurachi ◽  
H. Adachi ◽  
S. Ohtsuka ◽  
K. Morishige ◽  
K. Amemiya ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Mrna Level ◽  
Subcutaneous Fat ◽  
Rabbit Antiserum ◽  
Weight Ratio ◽  
The Body ◽  
Ovariectomized Mice ◽  
Liver And Kidney ◽  
Epidermal Growth

Ovariectomy (Ovx) of mice significantly increases the epidermal growth factor (EGF) concentration in the submandibular gland. To elucidate the role of this elevated EGF in obesity of Ovx mice, we examined the effects of sialoadenectomy (Sx) and anti-EGF rabbit antiserum administration on the body weight (BW) gain and carcass fat deposition in Ovx animals. Studies were performed in four groups of mice consisting of control, Ovx, Ovx+Sx, and Ovx+anti-EGF groups. Ovx increased the BW gain compared with the control animals, whereas Sx and anti-EGF significantly reduced it. Although the relative weights (weight ratio to BW) of the liver and kidney were not significantly changed by Ovx, Sx, or anti-EGF treatment of Ovx mice, the relative weights of mesenteric, parametrial, and subcutaneous fat tissues were increased in Ovx mice, and this increase was significantly reduced by Sx or anti-EGF administration. Ovx induced adipocyte hypertrophy, and this effect was eliminated by Sx and anti-EGF. Moreover, acyl-CoA synthetase mRNA level was increased by Ovx, and this increase was reduced by Sx and anti-EGF in mesenteric fat tissue. These findings suggest that elevation of EGF may play a role in the induction of obesity in Ovx mice.

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