scholarly journals Poor reliability of immunocytochemical localization of IgG in immersion-fixed tissue from the central nervous system.

1992 ◽  
Vol 40 (7) ◽  
pp. 987-991 ◽  
Author(s):  
R H Fabian
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Serum Proteins ◽  
Surrounding Tissue ◽  
Immunocytochemical Staining ◽  
Post Mortem ◽  
Fixed Tissue ◽  
The Central Nervous System ◽  
Fixation Interval

The effect of fixation technique and post mortem-to-fixation interval in immersion-fixed tissue from the central nervous system on immunocytochemical staining for the presence of an immunoglobulin was determined in mice. Immersion-fixed tissue was found to be inferior to perfusion-fixed tissue for immunocytochemical staining of this serum protein. Unlike what has been observed for other antigens, the quality of staining for IgG in immersion-fixed tissue decreased to unacceptable levels if the post mortem-to-fixation interval was increased to more than a few hours. This effect may be secondary to the rapid post-mortem disintegration of the blood-brain barrier and a resulting diffusion of serum proteins into surrounding tissue from the vasculature.

scholarly journals Diagnosis and treatment of Multiple sclerosis: Brazilian and global overview

2021 ◽  
Author(s):  
Lorrane de Moura Moreira ◽  
Bruna Stefany Alves Françozo ◽  
Bruno Barcelos Pereira ◽  
Camila Almeida Sardinha ◽  
Débora Pimenta Alves ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Central Nervous System ◽  
Nervous System ◽  
Research Result ◽  
Motor Action ◽  
The Central Nervous System ◽  
Nutritional Monitoring ◽  
Diagnosis Therapy ◽  
Inflammatory Character

Introduction: Multiple sclerosis (MS) is a neurodegenerative autoimmune disease of the central nervous system with a chronic, progressive and inflammatory character. In addition, it presents itself in a heterogeneous way, and can be as an isolated syndrome or as a recurrent remitter, in the first stage, or as progressive, in the second stage. The present work was developed with the objective of determining which is the best form of diagnosis and therapy for multiple sclerosis in Brazil. Methods: The review was performed in PubMed platform, with the descriptors: “multiple sclerosis”, “diagnosis”, “therapy” and “research”. Results: The research result in 148 articles. After a criterious reading and the application of the used criteria, was selected 20 articles. Conclusion: For the diagnosis of this chronic neurological disease, magnetic resonance imaging is used to assess myelination of the different regions of the central nervous system, which is the most suitable for the diagnosis of MS. Μoreover, as a complement, cerebrospinal fluid extraction and blood tests are performed in order to ascertain the concentration of B cells. Regarding therapeutics, this is diversified, including drugs, diets and therapies that stimulate cognition and motor action, such as the use of virtual reality programs and motor images. In relation to drugs, it is of importance that SUS makes natalizumab and ocrelizumab available because they are more efficient and enable users to have a better quality of life. Finally, nutritional monitoring is also suggested to establish a ketogenic or fasting diet in a balanced way

scholarly journals Quality of Life in Carotid Atherosclerosis: The Role of Co-morbid Mood Disorders

2016 ◽  
Vol 12 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Maria Lecca ◽  
Luca Saba ◽  
Roberto Sanfilippo ◽  
Elisa Pintus ◽  
Michela Cadoni ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Central Nervous System ◽  
Nervous System ◽  
Mood Disorders ◽  
Carotid Atherosclerosis ◽  
Short Form ◽  
The Central Nervous System ◽  
Dsm Iv

Introduction/Objective: To study in severe carotid atherosclerosis (CA): the frequency of mood disorders (MD); the impairment of quality of life (QoL); the role of co-morbid MD in such impairment. Methods: Case-control study. Cases: consecutive in-patients with CA (stenosis ≥ 50%). Controls: subjects with no diagnosis of CA randomized from a database of a community survey. Psychiatric diagnosis according to DSM-IV made by clinicians and semi-structured interview, QoL measured by the Short Form Health Survey (SF-12). Results: This is the first study on comorbidity on CA disease and MD in which psychiatric diagnoses are conducted by clinicians according to DSM-IV diagnostic criteria. Major Depressive Disorder (MDD) (17.4% vs 2.72%, P <0.0001) but not Bipolar Disorders (BD) (4.3% vs 0.5%, P = 0.99) was higher in cases (N=46) than in controls (N= 184). SF-12 scores in cases were lower than in controls (30.56±8.12 vs 36.81±6:40; p <0.001) with QoL comparable to serious chronic diseases of the central nervous system. The burden of a concomitant MDD or BD amplifies QoL impairment. Conclusion: Comorbid MD aggravates the impairment of QoL in CA. Unlike autoimmune diseases or degenerative diseases of the Central Nervous System, CA shows a strong risk of MDD than BD.

scholarly journals The Production of Rabbits free from Lesions Associated with Encephalitozoon Cuniculi

1968 ◽  
Vol 2 (2) ◽  
pp. 143-146 ◽  
Author(s):  
J. Mcc Howell ◽  
N. Edington
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Experimental Results ◽  
The Other ◽  
Post Mortem ◽  
Encephalitozoon Cuniculi ◽  
Post Mortem Examination ◽  
The Central Nervous System

Lesions produced by Encephalitozoon cuniculi are common in the central nervous system of laboratory rabbits, and may affect experimental results. Post-mortem examination often does disclosed that three manifested typical lesions, while the other seven did not. Of the fourteen young born to the three does in which lesions were discovered, four (29 per cent) were free from obvious evidence of the disease. Of the fifty young born to the seven lesion-free does, forty-seven (94 per cent) were also free from such lesions.

scholarly journals BIOLOGICAL MARKERS OF NON-CARCEROGENIC NEGATIVE IMPACTS ON THE CENTRAL NERVOUS SYSTEM OF CHILDREN IN THE AREA WITH EXPOSURE TO ALUMINUM PRODUCTION EMISSIONS

2018 ◽  
Vol 97 (5) ◽  
pp. 461-469
Author(s):  
I. G. Zhdanova-Zaplesvichko ◽  
Marina A. Zemlyanova ◽  
Yu. V. Koldibekova
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Aluminum Content ◽  
Negative Impact ◽  
Neuron Specific Enolase ◽  
Aluminum Production ◽  
Atmospheric Air ◽  
Hyperactivity Disorder ◽  
The Central Nervous System

Introduction. The priority indicator of the influence of chemical factors of the environment on the health of the population of Russia is the pollution of atmospheric air, the composition of which is largely determined by regional features of production. Material and methods. A comparative hygienic assessment of the quality of the atmospheric air of the territory with the placement of aluminum production and the territory without similar sources of emissions was conducted; a chemical-analytical and clinical laboratory examination of 135 children was carried out, with an assessment of the negative effects from the nervous system in the case of aerogenic exposure to aluminum and manganese. Results. In the conditions of the existing quality of atmospheric air in the residential area in the zone of influence of aluminum production which forms an aerogenic exposure of substances (aluminum and manganese), possessing of the unidirectional negative impact on the central nervous system, at the level of 0.0015g/(kg∙day) the share of aluminum is 93.3%, which indicates its primary impact on the population. Children of the observation group 1 showed urine aluminum content by 3.1 times more than in comparison to observation subgroup 2 and 6.9 times in relation to the comparison group (p = 0.0001). The concentration of aluminum as a marker of inhalation exposure is substantiated, and its value more than 0.053 mg/dm3 in urine may indicate an increased risk of neurotoxic exposure. An increased prevalence (1.6-5.5 times) of the negative impact on the CNS in the form of the asthenic autonomous syndrome, as a predictor of attention deficit and hyperactivity disorder, has been shown to be associated with the aerogenic exposure to aluminum. In children with an elevated aluminum content in urine comparing to the reference level, revealed laboratory abnormalities and indices were proved to be associated with an elevated concentration of aluminum in the urine relative to the children of the comparison group: an increase in the level of neuron-specific enolase in the serum indicating an increase in the activity of damage to the blood-brain barrier; an increase in the glutamic acid content by 1.3 times, characterizing the imbalance of the neurotransmitters of the central nervous system; reduction in serum phosphorus, reflecting the antagonistic effect of aluminum, followed by an increase in the level of ionized calcium in the blood. The contribution of aluminum to the biochemical and functional indices deviation from the physiological norm accounted for from 10% to 58%. On the basis of a consistent chain of reliable dependencies, a complex of biomarkers of the asthenic autonomous syndrome and attention deficit and hyperactivity disorder associated with an elevated aluminum content in urine, including glutamic acid, neuron-specific enolase, and phosphorus is substantiated.

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