scholarly journals PEG embedding for immunocytochemistry: application to the analysis of immunoreactivity loss during histological processing.

1993 ◽  
Vol 41 (3) ◽  
pp. 455-463 ◽  
Author(s):  
P Klosen ◽  
X Maessen ◽  
P van den Bosch de Aguilar
Keyword(s):  
Polyethylene Glycol ◽  
High Temperature ◽  
Intermediate Filaments ◽  
Experimental Research ◽  
Thin Sections ◽  
Paraffin Embedding ◽  
Histological Processing ◽  
Histological Staining ◽  
Neurotransmitter Enzymes

We have developed a protocol for the production and longterm storage of polyethylene glycol (PEG) sections for immunocytochemistry. Sections obtained by this protocol allow immunolabeling for many different antigens, such as intermediate filaments, macrophage markers, or neurotransmitter enzymes. Standard histological staining can also be performed on these sections. This fixation-embedding system may therefore be of interest for histopathology of rare specimens, as well as for experimental research. Multiple labeling can be performed either on the same section or on consecutive thin sections, thus allowing a more thorough analysis of precious experimental material. We compare the advantages of PEG vs cryostat or vibratome sections. This protocol has been used to study the inactivation of antigenicity by paraffin embedding. We have identified the infiltration by paraffin as the antigenicity inactivating step, not dehydration or high temperature as generally thought.

ALCOA ALUMINUM ALLOY 7050

Alloy Digest ◽  
1990 ◽  
Vol 39 (1) ◽  
Keyword(s):  
Corrosion Resistance ◽  
Aluminum Alloy ◽  
High Temperature ◽  
Magnesium Alloy ◽  
Stress Corrosion Cracking ◽  
Heat Treating ◽  
Thin Sections ◽  
Aerospace Applications ◽  
Temperature Performance ◽  
Aluminum Company

Abstract ALCOA ALUMINUM ALLOY 7050 is an aluminum-zinc-copper-magnesium alloy with a superior combination of strength, stress-corrosion cracking resistance and toughness, particularly in thick sections. In thin sections it also possesses an excellent combination of properties that are important for aerospace applications. This datasheet provides information on composition, physical properties, elasticity, and tensile properties as well as creep. It also includes information on low and high temperature performance, and corrosion resistance as well as forming, heat treating, and joining. Filing Code: Al-233. Producer or source: Aluminum Company of America. Originally published as Aluminum 7050, January 1979, revised January 1990.

Experimental Research of Gas Hydrate Drilling Fluids Using Polyethylene Glycol as an Inhibitor

2006 ◽  
Vol 17 (3) ◽  
pp. 276-282 ◽  
Author(s):  
L ZHANG ◽  
G JIANG ◽  
Y TU ◽  
J CAI ◽  
B DOU ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Experimental Research ◽  
Gas Hydrate ◽  
Drilling Fluids

Experimental Research on Bond Strength between Rebar and Concrete after High Temperature

2011 ◽  
Vol 71-78 ◽  
pp. 1057-1061 ◽  
Author(s):  
Ke Fang Yin ◽  
Yang Han ◽  
Yi Liu
Keyword(s):  
Reinforced Concrete ◽  
High Temperature ◽  
Bond Strength ◽  
Experimental Research ◽  
Bonding Strength ◽  
Different Temperatures ◽  
Ultimate Bond Strength

With the centrally pulling-out test, the bond strength of reinforced concrete is measured with different temperatures and different cooling ways after high temperature; and the ultimate bond strength and slip of reinforced and concrete under different conditions are analyzed. The results show that the bonding strength declines gradually with the increase of temperature, and the ultimate slippage also decreases gradually.

scholarly journals The behaviours of Mg and Ca in some types of ultramaphic alkaline melts in the presence of H 2 O at high temperature and pressure (the experimental data)

2002 ◽  
Vol 50 ◽  
Author(s):  
V. K. MARKOV ◽  
V.V. NASEDKIN ◽  
N. M. BOEVA
Keyword(s):  
High Temperature ◽  
High Temperature Phase ◽  
Optical Methods ◽  
Phase Identification ◽  
Temperature Phase ◽  
Ray Method ◽  
Thin Sections ◽  
Piston Cylinder ◽  
Structural Peculiarities ◽  
Main Components

The sample has been investigated, which was composed of olivine-melilite rock from the south-western part of the Kugda intrusion, situated in the eastern margin of the Maimecha-Kotuiskay alkaline- ultramaphic province (northern part of Siberia platform). The experiments were carried out using a high-pressure apparatus of the "piston-cylinder" type. The pressure was 5-15 kb and the temperature -800-1250 0 C. The water content in the system was 3 and 9 wt. All experiments were carried out in hermetically sealed Pt or Au ampoules. The samples were chemically analyzed using a scanning electron microscope (SEM). Besides, the chemical composition of the experimental phases was determined by means of more than 40 electron microprobe in 20 experimental samples. For phase identification the X-ray method was used too. The structural peculiarities of rocks and mineral compositions were studied by optical methods in polished and thin sections prior and after the experiment. It was shown that at pressures above 7.5-8 kb the olivine-melilite melt is separated into two melts: pyroxenite and melilite. Most part of Mg in this system was concentrated in a high temperature phase of diopside type. The remaining melt was depleted of Mg and enriched by Ca. The mineralization vapour practically did not contain Mg. The main components of the mineralization vapour were Ca, Na, Si and Fe.

The Research of the Chemistry Composition of High Temperature Oxidization under Loads for the Cr5Mo Alloy

2011 ◽  
Vol 694 ◽  
pp. 729-732
Author(s):  
Shun Qing Chen ◽  
Yu Min Ma
Keyword(s):  
High Temperature ◽  
Experimental Research ◽  
Experimental Results ◽  
The Difference

The chemistry composition of the high temperature oxidization under loads has been analyzed for the Cr5Mo alloy in this paper. The experimental research to the Fe, Cr and O elements have also been done. The difference between loads and no loads has been emphasized to the chemistry elements of the Cr5Mo alloy. The experimental results showed that the temperature couldn’t change the rate of the chemistry elements, but the loads could change them. The chemistry elements Fe ，Mo and Cr could change more obvious than other elements of the Cr5Mo alloy in this paper.

The prevention of dehydration injury in celery seeds by polyethylene glycol, abscisic acid, dark and high temperature

1982 ◽  
Vol 55 (4) ◽  
pp. 407-410 ◽  
Author(s):  
N. L. Biddington ◽  
P. A. Brocklehurst ◽  
A. S. Dearman ◽  
Jane Dearman
Keyword(s):  
Abscisic Acid ◽  
Polyethylene Glycol ◽  
High Temperature ◽  
Celery Seeds
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