Both B and T lymphocytes may be clonally involved in myelofibrosis with myeloid metaplasia

A combination of magnetic cell sorting (MACS) and fluorescent in situ hybridization (FISH) techniques was used to detect clonal cytogenetic markers in different myeloid and lymphoid cell types of the peripheral blood from 4 patients with myelofibrosis with myeloid metaplasia (MMM) that was associated with either a 13q− or a 20q− karyotypic abnormality. Interphase cytogenetics studies demonstrated abnormal clonal FISH signal patterns in neutrophil, myeloid, erythroid, megakaryocyte, and B- and T-cell preparations in 3 of the 4 patients. In one patient, FISH results were within normal limits in T cells and slightly abnormal in B cells. In general, the percentage of abnormal nuclei was variable in both lymphocyte populations but always higher in B lymphocytes compared with T lymphocytes. The current study provides direct evidence for the clonal involvement of both B and T lymphocytes in MMM. A larger study is needed to clarify the relevance of the observed interpatient heterogeneity in clonal constitution.

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Prognostic relevance of cytogenetics determined by fluorescent in situ hybridization in patients having myelofibrosis with myeloid metaplasia

Cancer ◽

10.1002/cncr.22318 ◽

2006 ◽

Vol 107 (12) ◽

pp. 2801-2806 ◽

Cited By ~ 13

Author(s):

Kathrin Strasser-Weippl ◽

Michael Steurer ◽

Mathias Kees ◽

Florian Augustin ◽

Alexandar Tzankov ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescent In Situ Hybridization ◽

Myeloid Metaplasia ◽

Prognostic Relevance ◽

Myelofibrosis With Myeloid Metaplasia

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Lymphocyte populations and apoptosis of peripheral blood B and T lymphocytes in children with end stage renal disease

Renal Failure ◽

10.3109/0886022x.2013.875833 ◽

2014 ◽

Vol 36 (4) ◽

pp. 502-507 ◽

Cited By ~ 14

Author(s):

Khaled Saad ◽

Khalid I. Elsayh ◽

Asmaa M. Zahran ◽

Karema M. Sobhy

Keyword(s):

T Lymphocytes ◽

Renal Disease ◽

Peripheral Blood ◽

End Stage Renal Disease ◽

Stage Renal Disease ◽

End Stage ◽

Lymphocyte Populations ◽

B And T Lymphocytes

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Comparison of peripheral blood interphase cytogenetics with bone marrow karyotype analysis in myelofibrosis with myeloid metaplasia

British Journal of Haematology ◽

10.1046/j.1365-2141.2001.03131.x ◽

2001 ◽

Vol 115 (2) ◽

pp. 316-319 ◽

Cited By ~ 23

Author(s):

Ayalew Tefferi ◽

Reid G. Meyer ◽

William A. Wyatt ◽

Gordon W. Dewald

Keyword(s):

Bone Marrow ◽

Peripheral Blood ◽

Karyotype Analysis ◽

Interphase Cytogenetics ◽

Myeloid Metaplasia ◽

Myelofibrosis With Myeloid Metaplasia

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Differential expression of somatostatin receptor subtypes in human peripheral blood mononuclear cell subsets

Acta Endocrinologica ◽

10.1530/eje.0.1500565 ◽

2004 ◽

pp. 565-577 ◽

Cited By ~ 36

Author(s):

EG Lichtenauer-Kaligis ◽

VA Dalm ◽

SP Oomen ◽

DM Mooij ◽

PM van Hagen ◽

...

Keyword(s):

T Lymphocytes ◽

Mrna Expression ◽

T Lymphocyte ◽

Peripheral Blood ◽

Human Peripheral Blood ◽

Lymphocyte Activation ◽

Cell Types ◽

Facs Analysis ◽

Peripheral Blood Mononuclear ◽

B And T Lymphocytes

BACKGROUND: Somatostatin (SS)-binding sites have been demonstrated in human lymphoid tissues and peripheral blood cells. However, not much is known with respect to the SS receptor subtype (sst) expression pattern and the expression of SS itself in the immune system. OBJECTIVE: The aim of this study was to evaluate the mRNA expression of the five known sst (sst(1-5)) in peripheral blood mononuclear cell (sub)populations. Moreover, the expression of the mRNAs encoding SS and the SS-like peptide cortistatin (CST) in immune cell subsets was studied. METHODS: RT-PCR and quantitative PCR were performed to evaluate sst, SS and CST mRNA expression in cells in the basal or activated state. Fluorescence-activated cell sorter (FACS) analysis using fluorescent SS was performed to visualize sst protein on cell membranes. RESULTS: B- and T-lymphocytes selectively expressed sst(3) mRNA. sst(3) expression in B-lymphocytes was significantly lower compared with T-lymphocytes. Unstimulated, freshly isolated monocytes did not express any sst mRNA. Upon activation, monocytes selectively expressed sst(2) mRNA, whereas T-lymphocyte activation upregulated sst(3) expression. sst(2) mRNA expression on monocytes was confirmed by FACS analysis. B- and T-lymphocytes did not express SS mRNA, while both cell types expressed CST mRNA. CST mRNA expression was downregulated following T-lymphocyte activation. CONCLUSION: We demonstrate for the first time unequivocally that human peripheral blood B- and T-lymphocytes selectively express sst(3), whereas monocytes do not express sst. However, upon activation, monocytes are induced to express sst(2A). No expression of SS mRNA was detected in any cell type, whereas all cell types expressed CST mRNA. The differential expression of sst and CST mRNA in lymphocytes and monocytes suggests a functional significance for the CST-sst interaction in immune cells, but further studies should be performed to evaluate the significance of sst and CST in these cells.

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Host-cell apoptosis inTaenia solium-induced brain granulomas in naturally infected pigs

Parasitology ◽

10.1017/s0031182008004678 ◽

2008 ◽

Vol 135 (10) ◽

pp. 1237-1242 ◽

Cited By ~ 15

Author(s):

C. S. SIKASUNGE ◽

I. K. PHIRI ◽

M. V. JOHANSEN ◽

A. L. WILLINGHAM ◽

P. S. LEIFSSON

Keyword(s):

T Lymphocytes ◽

Normal Cell ◽

Taenia Solium ◽

Cell Types ◽

Terminal Deoxynucleotidyl Transferase ◽

Tunel Staining ◽

Apoptotic Cells ◽

Host Cell Apoptosis ◽

Cellular Immune

SUMMARYTo assess whether apoptosis occurs in pig brain granulomas due toTaenia soliumcysticerci, brain tissues from 30 pigs naturally infected withT. soliumcysticercosis were evaluated by terminal deoxynucleotidyl transferase-end labelling (TUNEL) staining. In addition, tissues were stained with CD3 marker to identify T lymphocytes. Examination of TUNEL-stained tissues showed apoptotic cells in early lesions that contained viable cysticerci. Apoptotic cells were primarily found interspersed with normal cell types, and were mostly located in the inflammatory infiltrate. Late or advanced granulomas with disintegrated scolices did not show TUNEL-positive cells. CD3+ cells were found in both early and advanced lesions and apoptosis mainly co-localized with CD3+ T lymphocytes. This suggests that these cells are constantly undergoing apoptosis and thus die as soon as they arrive at the site of infection. Apoptosis indeed may be one way by whichT. soliumcysticerci down-regulate the host's cellular immune response in early cysticercosis. Therefore, further research is needed to establish if other cells besides T-lymphocytes are also a target for destruction by cysticerci in early cysticercosis as well as studies to assess if cysteine protease is expressed by viable cysticerciin situ.

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Differences between the carbohydrate units of cell-surface glycoproteins of moust B- and T-lymphocytes

Biochemical Journal ◽

10.1042/bj1810451 ◽

1979 ◽

Vol 181 (2) ◽

pp. 451-456 ◽

Cited By ~ 5

Author(s):

T Krusius ◽

J Finne ◽

L C Andersson ◽

C G Gahmberg

Keyword(s):

T Lymphocytes ◽

Cell Surface ◽

Affinity Chromatography ◽

Concanavalin A ◽

Cell Types ◽

Galactose Oxidase ◽

Neuraminidase Treatment ◽

Cell Surface Glycoproteins ◽

B And T Lymphocytes ◽

Surface Glycoproteins

Carbohydrate units of cell-surface glycoproteins of mouse B- and T-lymphocytes, labelled in their sialic acid residues by the periodate/NaB3H4 method and in their galactose residues by the galactose oxidase/NaB3H4 method after neuraminidase treatment, have been studied. Glycopeptides were prepared from the labelled cells by Pronase digestion and fractionated by concanavalin A affinity chromatography into two fractions (A and B). Alkali-labile oligosaccharides were isolated after mild NaOH/NaBH4 treatment by gel filtration. The alkali-labile oligosaccharides were further analysed by t.l.c. To study the relative proportion of neutral mannose-rich carbohydrate units (fraction C) in lymphocyte glycoproteins, glycopeptides were also prepared from unlabelled cells and subjected to concanavalin A affinity chromatography after N-[3H]acetylation of their peptide moiety. The major alkali-labile oligosaccharide component of both cell types was identified as galactosyl-(beta 1 leads to 3)-N-acetylgalactosaminitol. T-Lymphocytes were characterized by a high proportion of this oligosaccharide and a lower proportion of alkali-stable fraction A glycopeptides, whereas the opposite was observed for B-lymphocytes. The relative proportions of the concanavalin A-binding fractions B and C were similar in both cell types. The differences observed may correlate with the different surface properties of B- and T-lymphocytes.

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