scholarly journals Cycling Characteristics of Human Lymphocytes In Vitro

Blood ◽  
1974 ◽  
Vol 44 (1) ◽  
pp. 99-107 ◽  
Author(s):  
Lewis M. Schiffer ◽  
Arnold M. Markoe ◽  
Alan Winkelstein ◽  
Janet S. R. Nelson ◽  
John M. Mikulla
Keyword(s):  
Cell Cycle ◽  
Dna Polymerase ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Peripheral Blood Lymphocytes ◽  
Cell Cycle Time ◽  
Daughter Cells ◽  
Human Peripheral Blood Lymphocytes ◽  
A New Technique

Abstract The cycling characteristics of human peripheral blood lymphocytes in phytohemagglutinin-stimulated cultures were examined by means of a new technique employing radioautographic methods which assay the fraction of cells whose nuclei contain DNA polymerase utilizing exogenous and/or endogenous DNA primer-template capability. The fraction of cells labeled by these techniques increases 5-11 hr prior to the onset of DNA synthesis. Estimates of cell cycle time, G1 time, and fraction of cycling cells are made for the first 4 days in culture. Evidence is presented to support the hypothesis that daughter cells of the first divisions may be retired from cycle by virtue of loss of primer-template availability, rather than by loss of DNA polymerase.

scholarly journals Cytotoxic, clastogenic and genotoxic effects of cis-tetraammine(oxalato)ruthenium(III) dithionate on human peripheral blood lymphocytes

2020 ◽  
Vol 42 ◽  
pp. e50517
Author(s):  
Manuela da Rocha Matos Rezende ◽  
Vivianne de Souza Velozo-Sá ◽  
Cesar Augusto Sam Tiago Vilanova-Costa ◽  
Elisangela Silveira-Lacerda
Keyword(s):  
Comet Assay ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Low Frequency ◽  
Peripheral Blood Lymphocytes ◽  
Negative Control ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

There is a concern about stablishing the clinical risk of drugs used for cancer treatment. In this study, the cytotoxic, clastogenic and genotoxic properties of cis-tetraammine(oxalato)ruthenium(III) dithionite - cis-[Ru(C2O4)(NH3)4]2(S2O6), were evaluated in vitro in human lymphocytes. The mitotic index (MI), chromosomal aberrations (CA) and DNA damage by comet assay were also analyzed. The MTT test revealed that the ruthenium compound showed a slight cytotoxic effect at the highest concentration tested. The IC50 value for the compound after 24 hours of exposure was 185.4 µM. The MI values of human peripheral blood lymphocytes treated with 0.015, 0.15, 1.5 and 150 µM of cis-[Ru(C2O4)(NH3)4]2(S2O6) were 6.1, 3.9, 3.2 and 0.2%, respectively. The lowest concentration, 0.015 µM, did not show any cytotoxic activity. The CA values for the 0.015, 0.15 and 1.5 µM concentrations presented low frequency (1.5, 1.6 and 2.3%, respectively), and did not express clastogenic activity when compared to the negative control, although it was observed clastogenic activity in the highest concentration tested (150 µM). The results obtained by the comet assay suggest that this compound does not present genotoxic activity at lower concentrations. The results show that cis-[Ru(C2O4)(NH3)4]2(S2O6) has no cytotoxic, clastogenic or genotoxic in vitro effects at concentrations less than or equal to 0.015 µM. This information proves as promising in the treatment of cancer and is crucial for future trials.

Cytotoxic Effects of Trimethyltin Chloride on Human Peripheral Blood Lymphocytes in vitro

1989 ◽  
Vol 8 (5) ◽  
pp. 349-353 ◽  
Author(s):  
B.B. Ghosh ◽  
G. Talukder ◽  
A. Sharma
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Peripheral Blood Lymphocytes ◽  
Sister Chromatid Exchanges ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Abnormal Cells ◽  
Trimethyltin Chloride

Trimethyltin chloride was found to induce cytotoxic damage in vitro in human peripheral blood lymphocytes. Two concentrations (0.5 μg and 1.0 μg) were added to lymphocytes from male and female subjects in mitogen stimulated and serum supplemented culture medium for 72 h. A dose-related increase of inhibition of replication index (P < 0.01) and cell division (P < 0.001) was observed. The frequencies of abnormal cells and chromosomal aberrations such as chromatid and chromosome breaks, dicentrics, triradial and quadriradial configurations were increased significantly (P < 0.001), as were micronucleus counts (P < 0.001) and sister chromatid exchanges (P < 0.001). Endoreduplication, an extremely rare spontaneous event in human lymphocytes, was observed in a few cases at all dose levels. The effects were more pronounced in lymphocytes obtained from habitual smokers.

scholarly journals In vitro effect of tuibur (tobacco brew) on the viability of human blood lymphocytes

2017 ◽  
Vol 17 (1) ◽  
pp. 19-24 ◽  
Author(s):  
B. Lalruatfela ◽  
Jennifer Zoremsiami ◽  
Ganesh Chandra Jagetia
Keyword(s):  
Adverse Effect ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
In Vitro Study ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Long Time ◽  
Vitro Effect

The use of tobacco and its products are known to cause many illnesses including cancer. A smokeless tobacco locally manufactured called tuibur (tobacco brew) has been consumed by the Mizos from a very long time. In this experiment we aim to determine the cytotoxicity of tuibur by an in vitro study on tuibur-treated human peripheral blood lymphocytes. We have found that 24 h treatment of human lymphocytes with two grades of commercial tuibur and nicotine showed a concentration dependent decrease in cell viability. We, therefore, concluded that as the in vitro use of tuibur has an adverse effect on cell survival, its consumption might have potential side effects on the health of the users.

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