scholarly journals The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 280-284
Author(s):  
E Beutler ◽  
C West
Keyword(s):  
Shelf Life ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Donor ◽  
Red Cell ◽  
Packed Red Blood Cells ◽  
Citrate Phosphate Dextrose ◽  
Storage Cells ◽  
Stored Blood ◽  
Citrate Phosphate

The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

scholarly journals The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 280-284 ◽  
Author(s):  
E Beutler ◽  
C West
Keyword(s):  
Shelf Life ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Donor ◽  
Red Cell ◽  
Packed Red Blood Cells ◽  
Citrate Phosphate Dextrose ◽  
Storage Cells ◽  
Stored Blood ◽  
Citrate Phosphate

Abstract The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

scholarly journals Spectrin oxidation correlates with membrane vesiculation in stored RBCs

Blood ◽  
1987 ◽  
Vol 69 (6) ◽  
pp. 1777-1781 ◽  
Author(s):  
GM Wagner ◽  
DT Chiu ◽  
JH Qju ◽  
RH Heath ◽  
BH Lubin
Keyword(s):  
Shelf Life ◽  
Strong Correlation ◽  
Exchange Chromatography ◽  
Blood Bank ◽  
Disulfide Exchange ◽  
Citrate Phosphate Dextrose ◽  
Membrane Vesiculation ◽  
Stored Blood ◽  
Citrate Phosphate

Abstract An increase in spectrin oxidation in a variety of erythrocytes displaying a tendency to vesiculate has been previously described. To explore this relationship in more detail, we have studied blood stored in citrate-phosphate-dextrose-adenine under blood bank conditions because, in this system, vesiculation occurs slowly. Vesiculation was quantitated by measuring acetylcholinesterase release, and the extent of spectrin oxidation was detected by using thiol-disulfide exchange chromatography. A strong correlation (r = .92) was found between the extent of spectrin oxidation and vesiculation when blood from five donors was analyzed at weekly intervals during storage. This strongly suggests that spectrin oxidation plays a role in the formation of spectrin-free vesicles, thereby limiting the shelf life of stored blood.

scholarly journals Effect of storage on biochemical parameters of packed red blood cells of goats in............ 257-261 citrate phosphate dextrose adenine/ saline adenine glucose mannitol

2021 ◽  
Vol 52 (3) ◽  
Author(s):  
R. Thirupathy Venkatachalapathy ◽  
R. Thirupathy Venkatachalapathy ◽  
R. Thirupathy Venkatachalapathy ◽  
R. Thirupathy Venkatachalapathy ◽  
R. Thirupathy Venkatachalapathy
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
Reduced Glutathione ◽  
Packed Red Blood Cells ◽  
Storage Media ◽  
Citrate Phosphate Dextrose ◽  
Biochemical Studies ◽  
Citrate Phosphate ◽  
Packed Rbcs ◽  
Apparently Healthy

A study was conducted to assess the suitability of citrate phosphate dextrose adenine / saline adenine glucose mannitol as a storage media for packed RBCs of goats. Samples collected from ten apparently healthy goats were utilized for the study. Biochemical studies were carried out on day 0, 14, 28 and 42 days of storage using the parameters, viz. pH, glucose, potassium, malondialdehyde and reduced glutathione. The pH was stable throughout the study, whereas glucose showed significant reduction. Rest of the parameters increased significantly from 0th day to 42nd day. Based on the results, the storage media can be considered to be suitable for storing caprine packed RBCs.

scholarly journals Spectrin oxidation correlates with membrane vesiculation in stored RBCs

Blood ◽  
1987 ◽  
Vol 69 (6) ◽  
pp. 1777-1781
Author(s):  
GM Wagner ◽  
DT Chiu ◽  
JH Qju ◽  
RH Heath ◽  
BH Lubin
Keyword(s):  
Shelf Life ◽  
Strong Correlation ◽  
Exchange Chromatography ◽  
Blood Bank ◽  
Disulfide Exchange ◽  
Citrate Phosphate Dextrose ◽  
Membrane Vesiculation ◽  
Stored Blood ◽  
Citrate Phosphate

An increase in spectrin oxidation in a variety of erythrocytes displaying a tendency to vesiculate has been previously described. To explore this relationship in more detail, we have studied blood stored in citrate-phosphate-dextrose-adenine under blood bank conditions because, in this system, vesiculation occurs slowly. Vesiculation was quantitated by measuring acetylcholinesterase release, and the extent of spectrin oxidation was detected by using thiol-disulfide exchange chromatography. A strong correlation (r = .92) was found between the extent of spectrin oxidation and vesiculation when blood from five donors was analyzed at weekly intervals during storage. This strongly suggests that spectrin oxidation plays a role in the formation of spectrin-free vesicles, thereby limiting the shelf life of stored blood.

scholarly journals OBSERVATIONS ON THE BIOCHEMICAL EFFECTS OF TRANSFUSION OF CITRATE-PHOSPHATE-DEXTROSE STORED BLOOD IN MAN

1976 ◽  
Vol 86 (8) ◽  
pp. 1272-1279 ◽  
Author(s):  
Robert N. Miller ◽  
Robert Engelhardt ◽  
John A. Collins ◽  
Eduardo Slatopolsky ◽  
Jack H. Ladenson
Keyword(s):  
Biochemical Effects ◽  
Citrate Phosphate Dextrose ◽  
Stored Blood ◽  
Citrate Phosphate

scholarly journals Genetic differences in red cell osmotic fragility: analysis in allophenic mice

Blood ◽  
1982 ◽  
Vol 59 (5) ◽  
pp. 986-989 ◽  
Author(s):  
MJ Dewey ◽  
JL Brown ◽  
FS Nallaseth
Keyword(s):  
Phenotypic Variation ◽  
Blood Cells ◽  
Osmotic Fragility ◽  
Red Cells ◽  
Fragility Analysis ◽  
Red Cell ◽  
F1 Hybrid ◽  
Osmotic Lysis ◽  
Per Se ◽  
Genetically Determined

Abstract Mice of strain DBA/2J were found to produce red cells considerably more resistant to osmotic lysis than cells from C57BL/6J or the F1 hybrid between the two strains. Such strain-specific differences in osmotic fragility could be the result of genetically determined humoral or other systemic differences that indirectly influence red cell properties. Alternatively, this phenotypic variation might be an inherent property of the erythrocyte themselves and be directly controlled by their genotype. Analysis of red cells from allophenic (mosaic) mice of the strain composition C57BL/6J in equilibrium DBA/2J demonstrated that the latter possibility is the case. In such mice, erythrocytes of the DBA/2J genotype are relatively more resistant to osmotic lysis than are those of the C57BL/6J genotype; partial lysis of allophenic blood at intermediate salt concentrations results in marked enrichment for DBA/2J cells among the survivors. Future experiments designed to determine the mechanism underlying this difference can now focus on the properties of the red blood cells per se with the certainty that this property is inherent to the genotype of each cell.

Primary Plasma Therapy For Thrombotic Thrombocytopenic Purpura

1981 ◽  
Author(s):  
D C Case
Keyword(s):  
Platelet Count ◽  
Blood Cells ◽  
Reticulocyte Count ◽  
Fresh Frozen Plasma ◽  
Red Cell ◽  
Peripheral Smear ◽  
Plasma Therapy ◽  
Packed Red Blood Cells ◽  
Fresh Frozen ◽  
Frozen Plasma

A 25-year old male was admitted for an episode of right sided headache and subsequent generalized seizure. On admission his temperature was 37.6°. He had generalized petechiae and conjunctival hemorrhages. Organomegaly and lymphadenopathy were absent. There was mild left sided weakness. The Hgb. was 6.9 g/dl., reticulocyte count 10%, WBC 11,500/mm3, and platelet count 10,000/mm3. There were numerous schistocytes on the peripheral smear; bone marrow revealed panhyperplasia. Coagulation studies were normal. The BUN was 30, and the creatinine 1.7 mg/dl. Plasma was positive for Hgb. CT scan was negative for gross intracranial bleeding. The diagnosis of T.T.P. was made. On admission, the patient received 10 units of platelets and 2 units of packed red blood cells. He did not require further red cell or platelet transfusions during the rest of his hospital course. He was then started on infusions of fresh-frozen plasma. He then received one unit every 3 hours for 6 days, one unit every 6 hours for 2 days, then one unit every 12 hours for 2 days and finally 1 unit daily for 5 days. The response was immediate. After the infusions were started, the hematologic parameters steadily improved. The patient’s hematuria rapidly improved. Further CNS symptoms did not appear. The patient’s Hgb. was 12 g/dl, and reticulocyte count was 2.5% by the 9th day. His platelet count was normal by the 4th day. The patient was discharged on the 15th day. Infusions of plasma were discontinued at the time of discharge. The patient required plasma therapy 4 weeks later for recurrent thrombocytopenia (50,000/mm3). The patient has remained normal for 9 months since therapy and further plasma has not been required. Primary plasma therapy for T.T.P. as sole treatment should be further studied.

scholarly journals Normal Functions of the Spleen Relative to Red Blood Cells: A Review

Blood ◽  
1959 ◽  
Vol 14 (4) ◽  
pp. 399-408 ◽  
Author(s):  
WILLIAM H. CROSBY
Keyword(s):  
Cell Surface ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Normal Function ◽  
Red Cells ◽  
Red Cell ◽  
Cell Production ◽  
Normal Functions ◽  
Normal Spleen ◽  
And Storage

Abstract During all the stages of a red cell’s life the normal spleen exerts a normal function. Eight of these functions have been considered: (1) erythropoiesis; (2) an effect upon red cell production; (3) an effect upon maturation of the red cell surface; (4) the reservoir function; (5) the "culling function"; (6) iron turnover and storage; (7) the "pitting function"; (8) destruction of old red cells.

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