scholarly journals Effect of storage on biochemical parameters of packed red blood cells of goats in............ 257-261 citrate phosphate dextrose adenine/ saline adenine glucose mannitol

2021 ◽  
Vol 52 (3) ◽  
Author(s):  
R. Thirupathy Venkatachalapathy ◽  
R. Thirupathy Venkatachalapathy ◽  
R. Thirupathy Venkatachalapathy ◽  
R. Thirupathy Venkatachalapathy ◽  
R. Thirupathy Venkatachalapathy
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
Reduced Glutathione ◽  
Packed Red Blood Cells ◽  
Storage Media ◽  
Citrate Phosphate Dextrose ◽  
Biochemical Studies ◽  
Citrate Phosphate ◽  
Packed Rbcs ◽  
Apparently Healthy

A study was conducted to assess the suitability of citrate phosphate dextrose adenine / saline adenine glucose mannitol as a storage media for packed RBCs of goats. Samples collected from ten apparently healthy goats were utilized for the study. Biochemical studies were carried out on day 0, 14, 28 and 42 days of storage using the parameters, viz. pH, glucose, potassium, malondialdehyde and reduced glutathione. The pH was stable throughout the study, whereas glucose showed significant reduction. Rest of the parameters increased significantly from 0th day to 42nd day. Based on the results, the storage media can be considered to be suitable for storing caprine packed RBCs.

scholarly journals The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 280-284 ◽  
Author(s):  
E Beutler ◽  
C West
Keyword(s):  
Shelf Life ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Donor ◽  
Red Cell ◽  
Packed Red Blood Cells ◽  
Citrate Phosphate Dextrose ◽  
Storage Cells ◽  
Stored Blood ◽  
Citrate Phosphate

Abstract The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

scholarly journals The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 280-284
Author(s):  
E Beutler ◽  
C West
Keyword(s):  
Shelf Life ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Donor ◽  
Red Cell ◽  
Packed Red Blood Cells ◽  
Citrate Phosphate Dextrose ◽  
Storage Cells ◽  
Stored Blood ◽  
Citrate Phosphate

The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

Transfusing red blood cells stored in citrate phosphate dextrose adenine-1 for 28 days fails to improve tissue oxygenation in rats

1997 ◽  
Vol 25 (5) ◽  
pp. 726-732 ◽  
Author(s):  
Robert D. Fitzgerald ◽  
Claudio M. Martin ◽  
Glen E. Dietz ◽  
Gordon S. Doig ◽  
Richard F. Potter ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Tissue Oxygenation ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

scholarly journals The Contribution of Storage Medium and Membranes in the Microwave Dielectric Response of Packed Red Blood Cells Suspension

2020 ◽  
Vol 10 (5) ◽  
pp. 1702 ◽  
Author(s):  
Larisa Latypova ◽  
Gregory Barshtein ◽  
Dan Arbell ◽  
Yuri Feldman
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Dielectric Response ◽  
Microwave Frequency ◽  
Dielectric Strength ◽  
Storage Medium ◽  
Storage Lesion ◽  
Packed Red Blood Cells ◽  
Microwave Dielectric ◽  
Citrate Phosphate

During cold storage, packed red blood cells (PRBCs) undergo slow detrimental changes that are collectively termed storage lesion. The aging of the cells causes alterations in the composition of the storage-medium in the PRBC unit. In this paper, we present the comparison of the dielectric response of water in the primary (fresh) storage medium (citrate phosphate dextrose adenine solution, CPDA-1) versus the storage medium from three expired units of PRBCs. Dielectric response of the water molecules has been characterized by dielectric spectroscopy technique in the microwave frequency band (0.5–40 GHz). The dominant phenomenon is the significant increase of the dielectric strength and decrease the relaxation time τ for the samples of the stored medium in comparison with the fresh medium CPDA-1. Furthermore, we demonstrated that removing the ghosts from PRBC hemolysate did not cause the alteration of the dielectric spectrum of water. Thus, the contribution associated with water located near the cell membrane can be neglected in microwave dielectric measurements.

scholarly journals Prolonged Storage of Red Blood Cells for Transfusion in Citrate Phosphate Dextrose Adenine-1 Affects Their Viability

OALib ◽  
2015 ◽  
Vol 02 (09) ◽  
pp. 1-7
Author(s):  
Samuel Antwi-Baffour ◽  
Samuel Appiah Danso ◽  
Jonathan Adjei ◽  
Ransford Kyeremeh ◽  
Michael Mark Addae
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Prolonged Storage ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

scholarly journals Characteristics of resveratrol and serotonin on antioxidant capacity and susceptibility to oxidation of red blood cells in stored human blood in a time-dependent manner

2017 ◽  
Vol 46 (1) ◽  
pp. 272-283 ◽  
Author(s):  
Zübeyir Huyut ◽  
Mehmet Ramazan Şekeroğlu ◽  
Ragıp Balahoroğlu ◽  
Mehmet Tahir Huyut
Keyword(s):  
Antioxidant Capacity ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Time Dependent ◽  
Dependent Manner ◽  
Protective Effects ◽  
Activity Time ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate ◽  
Over Time

Objective In stored red blood cells (RBCs), which are used in diseases (e.g., acute blood loss and leukaemia), storage lesions arise by oxidative stress and other factors over time. This study investigated the protective effects of resveratrol and serotonin on stored RBCs. Methods Blood from each donor (n = 10) was placed in different bags containing 70 mL of citrate phosphate dextrose (total volume: 500 mL) and divided into three groups (n = 30): control, 60 µg/mL resveratrol, and 60 µg/mL serotonin. Malondialdehyde (MDA) and glutathione (GSH) levels, activity of glutathione peroxidase (GSH-Px), catalase, and carbonic anhydrase (CA), and susceptibility to oxidation in RBCs, and pH in whole blood were measured at baseline and on days 7, 14, 21, and 28. Results MDA levels and susceptibility to oxidation were increased in all three groups time-dependently, but this increase was greater in the serotonin group than in the other groups. Activity of GSH-Px, CAT, and CA, as well as GSH levels, were decreased in the control and serotonin groups time-dependently, but were significantly preserved in the resveratrol group. The pH was decreased in all groups time-dependently. Conclusion Our study shows that resveratrol attenuates susceptibility to oxidation of RBCs and protects their antioxidant capacity, and partially preserves CA activity time-dependently.

Apoptotic Microrna Profiling of Packed Red Blood Cells During Storage.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 3145-3145
Author(s):  
Meganathan Kannan ◽  
Sandhya Kulkarni ◽  
Chintamani D Atreya
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Conflicts Of Interest ◽  
Storage Period ◽  
Storage Condition ◽  
Packed Red Blood Cells ◽  
Cellular Expression ◽  
Underlying Mechanisms ◽  
Packed Rbcs

Abstract Abstract 3145 Poster Board III-82 Introduction During storage, RBCs undergo physiological changes often termed as storage lesions that adversely affect their survival in vivo, following transfusion. MicroRNAs (miRs), the negative regulators of cellular mRNAs, control cellular expression of genes relevant to differentiation and apoptosis via mRNA degradation or inhibition of translation. Recent reports indicate that matured red blood cells (RBC) contain diverse population of miRs in abundance. Understanding the role of miRs in RBC during storage would perhaps provide insights into the mechanisms associated with storage lesions. Methods In this study, we utilized a membrane-based array to obtain differential miR profiles of 52-apoptosis-associated miRs in packed RBCs during storage. The packed RBCs were obtained from the National Institutes of Health (NIH) blood bank and stored at appropriate storage condition (4-8°C) for up to 40 days. Samples were collected at days 0, 10, 20, 30 and day 40 and subjected to miR analysis. Our rationale is that since miRs are regulators of apoptosis, profiling of apoptosis associated miRs in packed RBCs during storage would provide the first step towards understanding the underlying mechanisms associated with storage lesions. Results Our miR analysis identified perturbation of six miRNAs during packed RBC storage. Two miRs remain at high levels throughout the RBC storage period studied while four miRs demonstrated an upward trend from day 0 to day 40 of storage. TarMir bioinformatics-based target gene identification for miR-96 identified CASPN1 mRNA as its target. The presence of CASPN1 mRNA was confirmed by RT-PCR. Although this observation tempts us to speculate that an interaction of CASPN1 with miR-96 in stored RBC is possible, further experimental verification of this interaction is warranted. Conclusion The differential microarray analysis presented here suggest that further refinement of miR profiling of stored red blood cells could be of value as predictive markers of ‘cellular status of RBC’ during storage. Future experimental analysis of selected mRNA-miR interactions in packed RBC during storage would provide insights into the mechanisms of storage lesions The findings and conclusions in this abstract have not been formally disseminated by the Food and Drug Administration and should not be construed to represent any Agency determination or policy. Disclosures No relevant conflicts of interest to declare.

scholarly journals In vitro assessment of time-dependent changes in red cell cytoplasmic antioxidants of donkey blood preserved in citrate phosphate dextrose adenine 1 anticoagulant

2020 ◽  
Vol 13 (4) ◽  
pp. 726-730
Author(s):  
Okereke Henry Nnamdi ◽  
Udegbunam Rita Ijeoma ◽  
Nwobi Lotanna Gilbert ◽  
Ezeobialu Henry Toochukwu ◽  
Udegbunam Sunday Ositadinma
Keyword(s):  
Oxidative Stress ◽  
Antioxidant System ◽  
Blood Cells ◽  
Protective Capacity ◽  
Citrate Phosphate Dextrose ◽  
Protective System ◽  
Primary Antioxidant ◽  
Antioxidant Markers ◽  
Citrate Phosphate

Background and Aim: Stored blood is continuously exposed to oxidative stress, which affects its antioxidant protective system. Erythrocytes are naturally armed with antioxidant protective capacity. Blood antioxidant system functions to protect the blood cells against oxidative damage by free radicals. However, during storage, blood is continuously exposed to oxidative stress, which affects its antioxidant system. The aim of this work was to investigate alteration in malondialdehyde (MDA) levels, reduced glutathione (glutathione reductase [GSH-Rd]), catalase (CAT), and superoxide dismutase (SOD) activities in stored donkey blood. Materials and Methods: Blood (250 ml) was drawn from four clinically healthy donkeys into citrate phosphate dextrose adenine 1 blood bags and preserved at 4°C. MDA, GSH-Rd, CAT, and SOD activities were assayed by colorimetric methods, over a period of 42 days. Results: The result showed that SOD enzyme activities significantly (p<0.05) increased by day 7 post-storage (PS) while MDA levels significantly (p<0.05) increased by day 21 PS. However, activities of GSH-Rd and CAT enzymes decreased (p<0.05) by day 21 PS. Pearson's product-moment correlation showed a negative correlation between the levels of MDA and enzymatic antioxidant markers (CAT and GSH-Rd). Conclusion: The findings revealed that GSH-Rd and CAT are the primary antioxidant defense markers in donkey red blood cells. The observed alterations in these principal antioxidants suggest a 14 days optimum keeping time of donkey blood for blood banking purposes.

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