scholarly journals Evidence for the interleukin-2 dependent expansion of leukemic cells in adult T cell leukemia

Blood ◽  
1987 ◽  
Vol 70 (5) ◽  
pp. 1407-1411 ◽  
Author(s):  
M Maeda ◽  
N Arima ◽  
Y Daitoku ◽  
M Kashihara ◽  
H Okamoto ◽  
...  
Keyword(s):  
T Cell ◽  
Receptor Gene ◽  
Interleukin 2 ◽  
Leukemic Cells ◽  
Type I ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
In Vitro Proliferation ◽  
Adult T Cell Leukemia

Abstract Interleukin 2 (IL-2) receptor/Tac antigen is abnormally expressed on cells of patients with adult T cell leukemia (ATL) caused by infection with human T lymphotropic virus type I (HTLV-I). Twenty-five patients with ATL were examined to determine whether their leukemic cells continued to show IL-2-dependent proliferation. In 21 patients, the in vitro proliferation of HTLV-I-infected nonleukemic T cell clones was found to be dependent on IL-2. However, clonality analysis based on T cell receptor gene rearrangement profiles and the site of HTLV-I provirus integration revealed IL-2-dependent growth in leukemic cells in four patients with ATL. These results provide evidence for the IL-2- dependent proliferation of leukemic cells in some ATL patients.

scholarly journals Evidence for the interleukin-2 dependent expansion of leukemic cells in adult T cell leukemia

Blood ◽  
1987 ◽  
Vol 70 (5) ◽  
pp. 1407-1411 ◽  
Author(s):  
M Maeda ◽  
N Arima ◽  
Y Daitoku ◽  
M Kashihara ◽  
H Okamoto ◽  
...  
Keyword(s):  
T Cell ◽  
Receptor Gene ◽  
Interleukin 2 ◽  
Leukemic Cells ◽  
Type I ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
In Vitro Proliferation ◽  
Adult T Cell Leukemia

Interleukin 2 (IL-2) receptor/Tac antigen is abnormally expressed on cells of patients with adult T cell leukemia (ATL) caused by infection with human T lymphotropic virus type I (HTLV-I). Twenty-five patients with ATL were examined to determine whether their leukemic cells continued to show IL-2-dependent proliferation. In 21 patients, the in vitro proliferation of HTLV-I-infected nonleukemic T cell clones was found to be dependent on IL-2. However, clonality analysis based on T cell receptor gene rearrangement profiles and the site of HTLV-I provirus integration revealed IL-2-dependent growth in leukemic cells in four patients with ATL. These results provide evidence for the IL-2- dependent proliferation of leukemic cells in some ATL patients.

Interleukin-2-mediated growth of leukemic cells in lymph nodes of patients with adult T-cell leukemia/ lymphoma

1996 ◽  
Vol 20 (2) ◽  
pp. 135-141 ◽  
Author(s):  
Kakushi Matsushita ◽  
Naomichi Arima ◽  
Toshinobu Fujiyoshi ◽  
Yasuhisa Daitoku ◽  
Shiroh Hidaka ◽  
...  
Keyword(s):  
T Cell ◽  
Lymph Nodes ◽  
Interleukin 2 ◽  
Leukemic Cells ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Adult T Cell Leukemia

scholarly journals Leukemic cells from some adult T-cell leukemia patients proliferate in response to interleukin-4

Blood ◽  
1988 ◽  
Vol 72 (4) ◽  
pp. 1182-1186 ◽  
Author(s):  
T Uchiyama ◽  
M Kamio ◽  
T Kodaka ◽  
S Tamori ◽  
S Fukuhara ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Growth ◽  
Proliferative Response ◽  
Interleukin 2 ◽  
Leukemic Cells ◽  
Interleukin 4 ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Receptor System ◽  
Adult T Cell Leukemia

Abstract The proliferative response of fresh peripheral blood leukemic cells from eight adult T cell leukemia (ATL) patients to interleukin-4 (IL-4) was studied to determine the possibility that the IL-4-mediated T-cell growth pathway is involved in the cell growth of leukemic cells in ATL. Resting lymphocytes from ten normal individuals did not proliferate in response to IL-4. Leukemic cells from two ATL patients did not respond to interleukin-2 (IL-2) or IL-4. Leukemic cells from two patients did respond to IL-2, but not to IL-4. In contrast, a strong proliferative response was observed in the IL-4 culture, but not in the IL-2 culture in the remaining four patients. Chromosome analysis of mitotic cells, performed in one of four patients, confirmed that the cells dividing in response to IL-4 were leukemic cells, but not activated normal lymphocytes. These results indicate the activation of IL-4/IL-4 receptor system in leukemic cells from some ATL patients and suggest the possible involvement of the system in the proliferation of leukemic cells and the leukemogenesis in ATL.

scholarly journals Autocrine growth of interleukin 2-producing leukemic cells in a patient with adult T cell leukemia

Blood ◽  
1986 ◽  
Vol 68 (3) ◽  
pp. 779-782 ◽  
Author(s):  
N Arima ◽  
Y Daitoku ◽  
S Ohgaki ◽  
J Fukumori ◽  
H Tanaka ◽  
...  
Keyword(s):  
T Cell ◽  
Interleukin 2 ◽  
Leukemic Cells ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Autocrine Growth ◽  
Short Term ◽  
Adult T Cell Leukemia ◽  
Spontaneous Proliferation ◽  
Short Term Culture

Abstract Leukemic cells in the peripheral blood of a patient with adult T cell leukemia (ATL), which expressed the Tac antigen/interleukin 2 (IL2) receptor, were investigated in vitro for autocrine growth by IL 2. The cells showed spontaneous proliferation in mitogen-free medium. The spontaneous proliferation of the cells was inhibited by monoclonal anti- IL 2 or anti-Tac antibody. These cells were found to produce messenger RNA for IL 2 and secrete IL 2 during short-term culture in the same medium. Recombinant IL 2 and IL 2 secreted by the cells enhanced the proliferation of the cells in a dose-dependent manner when added to the initial culture. These findings demonstrate that an autocrine mechanism by IL 2 is involved in the proliferation of ATL cells during short-term culture.

scholarly journals Interleukin-2 Receptor β-Chain (p70-75) Expressed on Leukemic Cells from Adult T Cell Leukemia Patients

1990 ◽  
Vol 81 (9) ◽  
pp. 902-908 ◽  
Author(s):  
Taiichi Kodaka ◽  
Takashi Uchiyama ◽  
Takayuki Ishikawa ◽  
Masanori Kamio ◽  
Rie Onishi ◽  
...  
Keyword(s):  
T Cell ◽  
Interleukin 2 ◽  
Leukemic Cells ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Adult T Cell Leukemia ◽  
Interleukin 2 Receptor ◽  
Β Chain

scholarly journals Deregulation of microRNA involved in hematopoiesis and the immune response in HTLV-I adult T-cell leukemia

Blood ◽  
2009 ◽  
Vol 113 (20) ◽  
pp. 4914-4917 ◽  
Author(s):  
Marcia Bellon ◽  
Yves Lepelletier ◽  
Olivier Hermine ◽  
Christophe Nicot
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Ex Vivo ◽  
Type I ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Hematopoietic Stem ◽  
Adult T Cell Leukemia ◽  
Infected Cells

Human T-cell leukemia virus type-I (HTLV-I) is the etiologic agent of adult T-cell leukemia (ATL), an aggressive lymphoproliferative disease. MicroRNAs (miRNAs) are differentially expressed during hematopoiesis and lineage commitment of hematopoietic stem cell progenitors (HSCPs). Here, we report aberrant expression of hematopoietic-specific miR-223, miR-181a, miR-150, miR-142.3p, and miR-155 in HTLV-I–infected cells in vitro and uncultured ex vivo ATL cells. Our results suggest that HTLV-I–infected cells have an unbalanced expression of miRNA that favors T-cell differentiation. We also found altered expression of miRNA previously recognized as innate immunity regulators: miR-155, miR-125a, miR-132, and miR-146. Strikingly, our data also revealed significant differences between ex vivo ATL tumor cells and in vitro HTLV-I cell lines. Specifically, miR-150 and miR-223 were up-regulated in ATL patients but consistently down-regulated in HTLV-I cell lines, suggesting that ATL cells and in vitro–established cells are derived from distinct cellular populations.

Development of a novel redirected T-cell–based adoptive immunotherapy targeting human telomerase reverse transcriptase for adult T-cell leukemia

Blood ◽  
2013 ◽  
Vol 121 (24) ◽  
pp. 4894-4901 ◽  
Author(s):  
Yukihiro Miyazaki ◽  
Hiroshi Fujiwara ◽  
Hiroaki Asai ◽  
Fumihiro Ochi ◽  
Toshiki Ochi ◽  
...  
Keyword(s):  
T Cell ◽  
Adoptive Immunotherapy ◽  
Receptor Gene ◽  
Cell Receptor ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Human Telomerase Reverse Transcriptase ◽  
Adult T Cell Leukemia

Key Points The efficacy and safety of a novel redirected T-cell–based adoptive immunotherapy targeting hTERT for patients with adult T-cell leukemia. hTERT-specific T-cell receptor gene-transduced CD8+ T cells lyse ATL cells, but not normal cells, both in vitro and in vivo.

scholarly journals Soluble interleukin 2 receptors in sera of Japanese patients with adult T cell leukemia mark activity of disease

Blood ◽  
1988 ◽  
Vol 71 (4) ◽  
pp. 1021-1026 ◽  
Author(s):  
N Yasuda ◽  
PK Lai ◽  
SH Ip ◽  
PC Kung ◽  
Y Hinuma ◽  
...  
Keyword(s):  
T Cell ◽  
Interleukin 2 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Normal Range ◽  
Cell Leukemia Virus Type ◽  
Adult T Cell Leukemia ◽  
Human T Cell

Abstract Serum concentrations of soluble interleukin 2 receptors (sIL 2R) were measured by an enzyme-linked immunosorbent assay (ELISA) in 30 patients with adult T cell leukemia (ATL), in 9 patients with other hematopoietic malignancies, and in 17 asymptomatic individuals seropositive for human T cell leukemia virus type I (HTLV-I). Sixty HTLV-I seronegative, age-matched controls showed a normal range of form 63.2 to 480.8 U/mL. All asymptomatic carriers of HTLV-I had sIL 2R in their sera within the normal range. sIL 2R in sera was not related to the anti-HTLV-I antibody titer. Eleven patients with acute ATL, a clinical phenotype with median survival rate of 4.4 months, had markedly elevated sIL 2R (11,100 to 99,000 U/mL), but eight patients with smoldering ATL had low sIL 2R values (less than 480.8 U/mL) comparable to controls. Eleven patients with chronic ATL had intermediate elevated levels of sIL 2R (480.8 to 37,300.0 U/mL). Serum levels of sIL 2R correlated with the number of ATL cells (r = 0.812) and CD25-positive cells (r = 0.725) circulating in the peripheral blood. Longitudinal studies performed in four patients with ATL showed significant correlation between serum concentration of sIL 2R and activity of the malignancy. These findings suggest that the level of sIL 2R in serum indicated tumor load and, possibly, prognosis.

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