scholarly journals Homozygous deletion of the retinoblastoma gene in an acute lymphoblastic leukemia (T) cell line

Blood ◽  
1990 ◽  
Vol 75 (3) ◽  
pp. 730-735
Author(s):  
J Cheng ◽  
P Scully ◽  
JY Shew ◽  
WH Lee ◽  
V Vila ◽  
...  

Human leukemia cell lines were examined for the status of the retinoblastoma (RB) protein by immunoblotting analysis using antibodies raised against the TrpE-RB fusion protein. One of 16 cell lines examined, the T-cell acute lymphoblastic leukemia (ALL) line HSB-2, lacked the 110-Kd RB protein. Southern blot analysis of genomic DNA extracted from HSB-2 cells showed a large homozygous deletion of the RB gene, stretching from exon 18 beyond exon 27. Northern blot analysis showed multiple, abnormal RB transcripts in HSB-2. A truncated protein (72 Kd) was detected with 35S-methionine labeling but not with 32P- orthophosphate labeling of the HSB-2 cells. The genomic deletion of greater than 85 kb DNA at the RB locus (13q14) was not detectable in the karyotype of the HSB-2 cells. Among the 16 human leukemia cell lines examined for the status of the RB gene, only one, the HSB-2 line, showed an abnormal RB protein. Further study of primary leukemia and lymphoma samples appears to be warranted.

Blood ◽  
1990 ◽  
Vol 75 (3) ◽  
pp. 730-735 ◽  
Author(s):  
J Cheng ◽  
P Scully ◽  
JY Shew ◽  
WH Lee ◽  
V Vila ◽  
...  

Abstract Human leukemia cell lines were examined for the status of the retinoblastoma (RB) protein by immunoblotting analysis using antibodies raised against the TrpE-RB fusion protein. One of 16 cell lines examined, the T-cell acute lymphoblastic leukemia (ALL) line HSB-2, lacked the 110-Kd RB protein. Southern blot analysis of genomic DNA extracted from HSB-2 cells showed a large homozygous deletion of the RB gene, stretching from exon 18 beyond exon 27. Northern blot analysis showed multiple, abnormal RB transcripts in HSB-2. A truncated protein (72 Kd) was detected with 35S-methionine labeling but not with 32P- orthophosphate labeling of the HSB-2 cells. The genomic deletion of greater than 85 kb DNA at the RB locus (13q14) was not detectable in the karyotype of the HSB-2 cells. Among the 16 human leukemia cell lines examined for the status of the RB gene, only one, the HSB-2 line, showed an abnormal RB protein. Further study of primary leukemia and lymphoma samples appears to be warranted.


2009 ◽  
Vol 33 (10) ◽  
pp. 1386-1391 ◽  
Author(s):  
Hiroaki Goto ◽  
Takuya Naruto ◽  
Reo Tanoshima ◽  
Hiromi Kato ◽  
Tomoko Yokosuka ◽  
...  

2020 ◽  
Vol 38 (4) ◽  
pp. 614-617
Author(s):  
Koshi Akahane ◽  
Takahiko Yasuda ◽  
Shinobu Tsuzuki ◽  
Fumihiko Hayakawa ◽  
Nobutaka Kiyokawa ◽  
...  

2019 ◽  
Vol 8 (11) ◽  
pp. 5274-5288 ◽  
Author(s):  
Atsushi Watanabe ◽  
Takeshi Inukai ◽  
Keiko Kagami ◽  
Masako Abe ◽  
Masatoshi Takagi ◽  
...  

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