Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains

DNA probes that identify genes coding for heat-labile type I (LT-I) and heat-stable type 1 (ST-I) enterotoxins, enteropathogenic Escherichia coli adherence factor (EAF), and Shigella-like, invasiveness (INV) are used to evaluate the sensitivity and specificity of stool blots in comparison with the sensitivity and specificity of colony blots in detecting enteropathoghens. The sensitivities of the probes in stool blots are 91.7% for the LT-I probe, 76.9% for the ST-I probes, 78.9% for the EAF probe, and 45.5% for the INV probe. The specificity of all probes is higher than 95%. In general, the stool blot method identifies as many if not more LT-I-, ST-I-, and EAF-producing E. coli infections than the colony blots. Key words: DNA probes, stool blots, enteropathogens, diagnosis.

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Does enteropathogenic Escherichia coli produce heat-labile enterotoxin, heat-stable enterotoxins a or b, or cholera toxin A subunits?

Infection and Immunity ◽

10.1128/iai.46.2.612-614.1984 ◽

1984 ◽

Vol 46 (2) ◽

pp. 612-614 ◽

Cited By ~ 7

Author(s):

S A Long-Krug ◽

C S Weikel ◽

K T Tiemens ◽

E L Hewlett ◽

M M Levine ◽

...

Keyword(s):

Escherichia Coli ◽

Cholera Toxin ◽

Enteropathogenic Escherichia Coli ◽

Toxin A ◽

Heat Stable ◽

Heat Labile

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Susceptibility of the Mouse Intestine to Heat-Stable Enterotoxin Produced by Enteropathogenic Escherichia coli of Porcine Origin

Applied Microbiology ◽

10.1128/aem.26.3.450-451.1973 ◽

1973 ◽

Vol 26 (3) ◽

pp. 450-451 ◽

Cited By ~ 3

Author(s):

Lucia Dobrescu ◽

C. Huygelen

Keyword(s):

Escherichia Coli ◽

Enteropathogenic Escherichia Coli ◽

Heat Stable ◽

Mouse Intestine ◽

Porcine Origin

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Production of heat-stable enterotoxin II by chicken clinical isolates of Escherichia coli

FEMS Microbiology Letters ◽

10.1016/0378-1097(93)90038-4 ◽

1993 ◽

Vol 109 (2-3) ◽

pp. 311-315

Author(s):

J Noriko Akashi

Keyword(s):

Escherichia Coli ◽

Clinical Isolates ◽

Heat Stable

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Occurrence of Enteropathogenic Escherichia coli Serotypes in Butter

Journal of Food Protection ◽

10.4315/0362-028x-50.10.829 ◽

1987 ◽

Vol 50 (10) ◽

pp. 829-831 ◽

Cited By ~ 7

Author(s):

F. M. ABBAR ◽

M. TAHIR MOHAMED

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Fecal Coliforms ◽

Enteropathogenic Escherichia Coli ◽

Heat Stable ◽

Resistance Patterns ◽

Antibiotic Resistance Patterns

A total of 30 samples of butter analysed during the course of the investigation showed that fecal coliforms were absent from only 13.3% of samples. One hundred forty colonies of fecal coliforms were biochemically characterized with the following types obtained (Escherichia sp. 41.4%, Enterobacter sp. 25.7%, Citrobacter sp. 20%, Klebsiella sp. 10%). Five different serotypes, namely 0 125 K70(2), 0 142K86(1), 0 127K63(1), 0 114 K90(2), 0 111 K58(1) were detected in 7 of 58 Escherichia coli isolates and 51 strains were untypable. Three strains produced heat stable (ST) enterotoxin and belonged to the enteropathogenic serotype. The antibiotic resistance patterns of coliform strains are presented.

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