Inhibitory effects of inhaled complex traditional Chinese medicine on early and late asthmatic responses induced by ovalbumin in sensitized guinea pigs

Astragaloside IV (AS-IV) is one of the active ingredients in Astragalus membrananceus (Huangqi), a traditional Chinese medicine. The present study investigated the effects of AS-IV on Ca[Formula: see text] handling in cardiac myocytes to elucidate its possible mechanism in the treatment of cardiac disease. The results showed that AS-IV at 1 and 10[Formula: see text][Formula: see text]M reduced KCl-induced [Ca[Formula: see text]]i increase ([Formula: see text] from 1.33[Formula: see text][Formula: see text][Formula: see text]0.04 (control, [Formula: see text] 28) to 1.22[Formula: see text][Formula: see text][Formula: see text]0.02 ([Formula: see text], [Formula: see text] 29) and 1.22[Formula: see text][Formula: see text][Formula: see text]0.02 ([Formula: see text] 0.01, [Formula: see text]), but it enhanced Ca[Formula: see text] release from SR ([Formula: see text] from 1.04[Formula: see text][Formula: see text][Formula: see text]0.01 (control, [Formula: see text]) to 1.44[Formula: see text][Formula: see text][Formula: see text]0.03 ([Formula: see text], [Formula: see text]) and 1.60[Formula: see text][Formula: see text][Formula: see text]0.04 ([Formula: see text] 0.01, [Formula: see text]0), in H9c2 cells. Similar results were obtained in native cardiomyocytes. AS-IV at 1 and 10[Formula: see text][Formula: see text]M inhibited L-type Ca[Formula: see text] current ([Formula: see text] from [Formula: see text]4.42[Formula: see text][Formula: see text][Formula: see text]0.58 pA/pF of control to [Formula: see text]2.25[Formula: see text][Formula: see text][Formula: see text]0.12 pA/pF ([Formula: see text] 0.01, [Formula: see text] 5) and [Formula: see text]1.78[Formula: see text][Formula: see text][Formula: see text]0.28 pA/pF ([Formula: see text] 0.01, [Formula: see text] 5) respectively, when the interference of [Ca[Formula: see text]]i was eliminated due to the depletion of SR Ca[Formula: see text] store by thapsigargin, an inhibitor of Ca[Formula: see text] ATPase. Moreover, when BAPTA, a rapid Ca[Formula: see text] chelator, was used, CDI (Ca[Formula: see text]-dependent inactivation) of [Formula: see text] was eliminated, and the inhibitory effects of AS-IV on ICaL were significantly reduced at the same time. These results suggest that AS-IV affects Ca[Formula: see text] homeostasis through two opposite pathways: inhibition of Ca[Formula: see text] influx through L-type Ca[Formula: see text] channel, and promotion of Ca[Formula: see text] release from SR.

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A nebulized complex traditional Chinese medicine inhibits Histamine and IL-4 production by ovalbumin in guinea pigs and can stabilize mast cells in vitro

BMC Complementary and Alternative Medicine ◽

10.1186/1472-6882-13-174 ◽

2013 ◽

Vol 13 (1) ◽

Cited By ~ 9

Author(s):

Hung-Chou Chang ◽

Cheng-Chung Gong ◽

Chi-Lim Chan ◽

Oi-Tong Mak

Keyword(s):

Chinese Medicine ◽

Mast Cells ◽

Traditional Chinese Medicine ◽

Guinea Pigs

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Inhibitory Effects of Traditional Chinese Medicine Shimotsu-to and Its Included Crude Fractions on Adjuvant-Induced Chronic Inflammation of Mice.

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.19.47 ◽

1996 ◽

Vol 19 (1) ◽

pp. 47-52 ◽

Cited By ~ 14

Author(s):

Satoru KOJIMA ◽

Kazuhiko INABA ◽

Shinjiro KOBAYASHI ◽

Masayasu KIMURA

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Chronic Inflammation ◽

Inhibitory Effects

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Macromolecular substances as a dangerous factor in traditional Chinese medicine injections were determined by size-exclusive chromatography

Toxicology Research ◽

10.1093/toxres/tfaa024 ◽

2020 ◽

Vol 9 (3) ◽

pp. 323-330

Author(s):

Yue Li ◽

Jinlian Duan ◽

Heng Xia ◽

Bin Shu ◽

Weigang Duan

Keyword(s):

Molecular Weight ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Guinea Pigs ◽

Anaphylactoid Reaction ◽

Danshen Injection ◽

Anaphylactoid Reactions ◽

Qingkailing Injection ◽

The Relationship ◽

Do So

Abstract Macromolecular substances in traditional Chinese medicine injections (TCMIs) are expected to be a main dangerous factor causing anaphylactic or anaphylactoid reaction. The main aim of the study was to verify the macromolecular substances’ anaphylactic or anaphylactoid reaction in guinea pigs and establish a size-exclusive chromatographic method to detect them. The macromolecular substances from six TCMIs (Danshen injection, Dengzhanxixin injection, Honghua injection, Qingkailing injection, Shuanghuanglian injection and Shuxuening injection) were prepared by removing substances with molecular weight less than 10 kDa with an ultra-filter. The anaphylactic and anaphylactoid reactions caused by original TCMIs, injections rich in or free of macromolecules were assayed in guinea pigs. The relationship between the amount of the macromolecular substances and peak area of chromatogram was established by size-exclusive chromatography. Injections free of macromolecules were not likely to cause anaphylactic and anaphylactoid reactions, but injections rich in macromolecular substances were more likely to do so. If the macromolecular substances with molecular weight bigger than 10 kDa were removed, the signal of macromolecular substances in TCMIs was quantitatively reduced. All the results suggested that macromolecular substances in TCMIs are a dangerous factor causing safety problems, and the macromolecular substances can be quantitatively detected with size-exclusive chromatography.

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A study on the traditional chinese medicine jinyebaidu for prevention and treatment of intrauterine infection with guinea pigs cytomegalovirus

Journal of Huazhong University of Science and Technology [Medical Sciences] ◽

10.1007/bf02896182 ◽

2005 ◽

Vol 25 (6) ◽

pp. 721-723 ◽

Cited By ~ 4

Author(s):

Chen Suhua ◽

Xiong Jinwen ◽

Xing Wei ◽

Wen Liangzhen ◽

Liu Haizhi ◽

...

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Guinea Pigs ◽

Intrauterine Infection ◽

Prevention And Treatment

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Study on the Inhibitory Effects of Ephedra Aconite Asarum Decoction on LPS-Induced Dendritic Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/3272649 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Wenting Ji ◽

Jinghong Hu ◽

Xue Yu ◽

Lanlan Zhang ◽

Min Liu ◽

...

Keyword(s):

Bone Marrow ◽

Dendritic Cells ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Inflammatory Responses ◽

Inhibitory Effects ◽

Chinese Medicine Formula ◽

Th1 Polarization ◽

Anti Inflammation

Dendritic cells (DCs) can secrete cytokines stimulated by lipopolysaccharide (LPS), which leads to not just acute inflammatory responses but also Th1 polarization. Furtherly, chronic inflammation or autoimmune diseases could be triggered. As a classic Traditional Chinese Medicine formula, Ephedra Aconite Asarum Decoction with the main ingredients of ephedrine and hypaconitine can show effect on anti-inflammation and immunoregulation. But it remains unclear whether Ephedra Aconite Asarum Decoction controls DCs. In this study, we investigated the effects of Ephedra Aconite Asarum Decoction on LPS-induced bone marrow-derived DCs (BMDCs) in vitro. We found that Ephedra Aconite Asarum Decoction lowered surface costimulators on DCs and reduced the expression of Th1 type cytokines. Yet it is slightly beneficial for shifting to Th2. Our work reveals that the Ephedra Aconite Asarum Decoction can regulate Th1 inflammation through intervening DCs.

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