scholarly journals The effects of varicocelectomy on the DNA fragmentation index and other sperm parameters: a meta-analysis

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Ponco Birowo ◽  
J. Rahendra Wijaya ◽  
Widi Atmoko ◽  
Nur Rasyid
Keyword(s):  
Dna Fragmentation ◽  
Semen Analysis ◽  
Meta Analysis ◽  
Sperm Concentration ◽  
Mean Difference ◽  
Sperm Parameters ◽  
Progressive Motility ◽  
Fragmentation Index ◽  
Common Causes ◽  
Dna Fragmentation Index

Abstract Background Varicocele is one of the most common causes of reversible male infertility, and 15% of the varicocele patients with normal semen analysis are diagnosed as infertile. According to the current guidelines, varicocelectomy is indicated based on abnormal sperm parameters and not abnormal DNA fragmentation index (DFI) values. Thus, in this study, we performed a meta-analysis of the effects of varicocelectomy on the DFI and other conventional sperm parameters, and determined whether DFI could be used to indicate varicocelectomy for varicocele patients. Results Through an electronic search of the PubMed, Scopus, EBSCO, and Cochrane databases, we included 7 prospective studies including a total of 289 patients in this meta-analysis. The results showed that varicocelectomy significantly reduced DNA fragmentation (mean difference: − 6.86; 95% confidence interval [CI]: − 10.04, − 3.69; p < 0.00001) and improved sperm concentration (mean difference: 9.59; 95% CI: 7.80, 11.38; p < 0.00001), progressive motility (mean difference: 8.66; 95% CI: 6.96, 10.36; p < 0.00001), and morphology (mean difference: 2.73; 95% CI: 0,65, 4.80; p = 0.01). Conclusion Varicocelectomy reduced DNA fragmentation and improved sperm concentration, progressive motility, and morphology. Additionally, the analysis showed that an abnormal DFI measurement should be considered as an indication for varicocelectomy.

The effects of Pb on sperm parameters and sperm DNA fragmentation of men investigated for infertility

2020 ◽  
Vol 31 (4) ◽  
Author(s):  
G.U.S. Wijesekara ◽  
D.M.S. Fernando ◽  
S. Wijeratne
Keyword(s):  
Dna Fragmentation ◽  
Seminal Plasma ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
World Health ◽  
Sperm Dna Fragmentation ◽  
Progressive Motility ◽  
Sperm Dna ◽  
The Mean ◽  
Pb Concentration

AbstractBackgroundLead (Pb) is one of the metals most prevalent in the environment and is known to cause infertility and deoxyribonucleic acid (DNA) fragmentation. This study aimed to determine the association between seminal plasma Pb and sperm DNA fragmentation in men investigated for infertility.MethodsMale partners (n = 300) of couples investigated for infertility were recruited after informed consent was obtained. Sperm parameters were assessed according to the World Health Organization (WHO) guidelines. Seminal plasma Pb was estimated by atomic absorption spectrophotometry after digestion with nitric acid.ResultsIn Pb-positive and -negative groups the sperm parameters and sperm DNA fragmentation were compared using independent sample t-test and the Mann-Whitney U-test, respectively. The mean [standard deviation (SD)] age and duration of infertility were 34.8 (5.34) years and 45.7 (35.09) months, respectively, and the mean Pb concentration was 15.7 μg/dL. In Pb positives compared to Pb negatives the means (SD) of sperm count, progressive motility viability and normal morphology were lower (p > 0.05) but the DNA fragmentation was significantly higher 39.80% (25.08) than Pb negatives 22.65% (11.30). Seminal plasma Pb concentration and sperm DNA fragmentation had a positive correlation (r = 0.38, p = 0.03). A negative correlation was observed between sperm DNA fragmentation and sperm concentration, progressive motility, total motility and viability. When the DNA fragmentation was ≥30% sperm concentration and viability decreased (p < 0.05).ConclusionsPb in seminal plasma had a significant effect on sperm DNA fragmentation but not with other sperm parameters.

Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay

Zygote ◽  
2003 ◽  
Vol 11 (4) ◽  
pp. 367-371 ◽  
Author(s):  
Isabelle Oger ◽  
Christelle Da Cruz ◽  
Gilles Panteix ◽  
Yves Menezo
Keyword(s):  
Dna Fragmentation ◽  
Chromatin Structure ◽  
Inverse Relationship ◽  
Sperm Concentration ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Oxidative Products ◽  
Dna Fragmentation Index

In our work, we have used 8-hydroxy-deoxyguanosine (8-OH-dG), one of the major oxidative products of sperm DNA, in a population of patients consulting for infertility. We found an inverse relationship between sperm concentration and the log of the ratio of 8-OH-dG to dG (P<0.01). On the same patients' sperm samples, the sperm chromatin structure assay (SCSA) was performed. An inverse relationship was observed between the DNA fragmentation index and sperm concentration (P<0.001). There was also a positive relationship between SCSA and log 8-OH-dG/dG. This indicates that DNA fragmentation measured by the SCSA originates in part from oxidative products. In a few patients, antioxidant treatment decreased the DNA fragmentation index below the threshold of 30% that is crucial for subfertility.

scholarly journals From Spermiogram to Bio-Functional Sperm Parameters: When and Why Request Them?

2020 ◽  
Vol 9 (2) ◽  
pp. 406 ◽  
Author(s):  
Rosita A. Condorelli ◽  
Aldo E. Calogero ◽  
Giorgio I. Russo ◽  
Sandro La Vignera
Keyword(s):  
Normal Form ◽  
Sperm Motility ◽  
Sperm Count ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
Sperm Parameter ◽  
Progressive Motility ◽  
Semen Volume ◽  
Chromatin Compactness

The aim of this experimental study was to evaluate whether infertile patients may benefit from the evaluation of bio-functional sperm parameters in addition to the conventional semen analysis. To accomplish this, we evaluated the correlation between conventional and bio-functional sperm parameters based on their percentile distribution in search of a potential threshold of these latter that associates with conventional sperm parameter abnormalities. The study was conducted on 577 unselected patients with infertility lasting at least 12 months. We identified cut-off values according to the median of the population for mitochondrial membrane potential (MMP), number of alive spermatozoa, and chromatin abnormality. High MMP (HMMP) (≥46.25%) was associated with sperm concentration, sperm count, progressive motility, and normal form. Low MMP (LMMP) (≥36.5%) was found to be associated with semen volume, sperm concentration, total sperm count, progressive motility, total motility, and normal form. The number of alive spermatozoa (≥71.7%) was associated with sperm concentration and progressive motility whereas abnormal chromatin compactness (≥21.10%) was associated with sperm concentration, total sperm count, and progressive motility. The data would suggest that, for every increase in the percentile category of sperm concentration, the risk of finding an HMMP≤46.25 is reduced by 0.4 and by 0.66 for a total sperm count. This risk is also reduced by 0.60 for every increase in the percentile category of sperm progressive motility and by 0.71 for total sperm motility. Each increment of percentile category of the following sperm parameter was followed by a decrease in the risk of finding an LMMP≤36.5: sperm concentration 1.66, total sperm count 1.28, sperm progressive motility 1.27, total sperm motility 1.76, and normal form 1.73. Lastly, the data showed that, for every increase in the percentile category of total sperm count, the risk of finding an abnormal chromatin compactness ≤21.10 is reduced by 1.25 (1.04–1.51, p < 0.05) and an increase of total sperm motility is associated with a reduced risk by 1.44 (1.12–1.85, p < 0.05). Results suggest a correlation between bio-functional and conventional sperm parameters that impact the sperm fertilizing potential. Therefore, the evaluation of bio-functional sperm parameters by flow cytometry may be useful to explain some cases of idiopathic male infertility.

313 ASSESSMENT OF SPERM DNA FRAGMENTATION IN CANINE EJACULATES USING THE Sperm-Halomax® KIT: PRELIMINARY RESULTS

2010 ◽  
Vol 22 (1) ◽  
pp. 312 ◽  
Author(s):  
M. Hidalgo ◽  
M. R. Murabito ◽  
M. J. Gálvez ◽  
S. Demyda ◽  
L. J. De Luca ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Commercial Kit ◽  
Sperm Dna Fragmentation Index ◽  
Dna Fragmentation Index

Recently, a new procedure for the analysis of sperm DNA fragmentation has been developed for humans and different mammalian species, using a commercial kit based on the sperm chromatin dispersion (SCD) test; however, a descriptive study in canine semen has not been performed. The aim of this work was to assess the sperm DNA fragmentation in canine ejaculates using the SCD test and 2 different staining techniques. For this purpose, ejaculates were collectedby digital manipulation from4 healthy dogs of different breeds (1 German Pointer, 2 Spanish Greyhounds, and 1 Crossbreed). After collection, the sperm-rich fraction of the ejaculates from 3 dogs were pooled each time (n = 4) and then extended in Dulbecco’s phosphate buffered saline. All the pooled semen samples presented physiological values concerning routine semen parameters (motility, morphology, and sperm concentration). The sperm DNA fragmentation was assessed using the Sperm-Halomax® commercial kit specifically developed for canine semen (Halotech DNA SL, Madrid, Spain). Two semen aliquots of the diluted pooled semen samples were processed on each pre-treated slide provided in the kit following the manufacturer’s instructions. The last step was the staining technique. We stained each slide with 2 different staining procedures. The first half of the slide was stained with propidium iodide (Sigma-Aldrich, St. Louis, MO, USA) mixed in a proportion 1 : 1 with an antifading solution. The second half of the slide was stained for 15 min in Wright solution (1.01383.0500, Merck, Whitehouse Station, NJ, USA) 1 :1 in Phosphate Buffer pH 6.88 (1.07294.1000, Merck). The stained slides were observed using fluorescence and light microscopy, respectively. Five hundred sperm per slide were counted. Spermatozoa with fragmented DNA showed a large and spotty halo of chromatin dispersion. Unfragmented sperm only showed a small and compact halo. Statistical analyses were performed using the Statistical Package for Social Science version 12.0 (SPSS Inc., Chicago, IL, USA). The sperm DNA fragmentation index was compared between Wright and fluorescence staining methods by ANOVA. Results were expressed as mean ± standard error of the mean. The first report of the sperm DNA fragmentation index in canine ejaculates was 2.26 ± 0.53% for Wright staining and 1.99 ± 0.10% for fluorescence technique. No differences were found between staining procedures. In conclusion, it was possible to assess the sperm DNA fragmentation of canine ejaculates using 2 different staining procedures, expecting that continuous research could be useful in defining the role of DNA fragmentation SCD test in canine semen evaluation and cryopreservation.

scholarly journals DNA fragmentation index and its correlation with other sperm parameters in 4833 infertile males

2018 ◽  
Vol 7 (9) ◽  
pp. 3718
Author(s):  
Priya S. Patil ◽  
Satish M. Patki ◽  
Shweta S. Patki ◽  
Ujjwala S. Patki
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Sperm Count ◽  
Semen Analysis ◽  
Pearson Correlation ◽  
Significant Negative Correlation ◽  
Male Factor ◽  
Fragmentation Index ◽  
Success Of Treatment ◽  
Dna Fragmentation Index

Background: Semen analysis forms the basic investigation for male factor of infertility. According to WHO Manual sperm values of normalcy are decreasing. Recent studies have highlighted newer parameters of sperm playing their role in the outcome of pregnancy. The aim was to study the DNA fragmentation index (DFI) and to know its correlation with other sperm parameters.Methods: A total of 4833 cases underwent routine semen analysis along with the DFI. The statistical analysis was done using SPSS using t test and ANOVA test.Results: It was observed that 52% infertile males presented late for treatment. All parameters showed a decline with advancing age. There was definite correlation between sperm count and sperm motility as well as morphology. The DFI showed significant negative correlation with sperm count. Pearson correlation showed positive correlation between DFI and sperm motility and morphology.Conclusions: The study highlights the need for increasing awareness about male factor of infertility. A thorough semen analysis along with DFI can help the clinician to explain the couple in a better way about the choice of ART and success of treatment.

scholarly journals Associations of Sperm mtDNA copy number, DNA fragmentation index, and reactive oxygen species with clinical outcomes in ART treatments

2021 ◽  
Author(s):  
Wei-Hui Shi ◽  
Mu-Jin Ye ◽  
Zhi-Yang Zhou ◽  
Ning-Xin Qin ◽  
Xuan-You Zhou ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Clinical Outcomes ◽  
Dna Fragmentation ◽  
Pregnancy Outcomes ◽  
Copy Number ◽  
Reactive Oxygen ◽  
Sperm Parameters ◽  
Oxygen Species ◽  
Fragmentation Index ◽  
Dna Fragmentation Index

Abstract Background Except for sperm parameters (sperm concentration, motility, and morphology), sperm mitochondria DNA copy number (mtDNA-CN), DNA fragmentation index (DFI), and reactive oxygen species (ROS) content are essential characteristics for sperm function. However, the roles of these measurements in embryo development and pregnancy outcomes are still unclear. Methods Semen samples of 402 participants were collected. Sperm parameters, including sperm morphology, concentration, and motility, were analyzed by a computer-assisted sperm analysis system. MtDNA-CN, DFI, and ROS levels were measured using remained sperms through quantitative polymerase chain reaction method, sperm chromatin structure assay and Reactive Oxygen Species Assay Kit, respectively. The assisted reproductive technology (ART) treatments, including in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), were performed in 126 couples of these participants. Results In 402 semen samples, elevated mtDNA-CN and DFI were associated with poor seminal quality. In 126 couples conducted ART, only mtDNA-CN was negatively correlated with fertilization rate in ART cases. However, it was not significant after adjusting male age, female age, seminal quality, and ART strategy. With regard to pregnancy outcomes, none of sperm mtDNA-CN, ROS and DFI was associated with clinical pregnancy rate in 79 cases transferred embryos. Conclusions Increased mtDNA-CN and DFI in sperms jointly contributed to poor seminal quality. However, none of sperm mtDNA-CN, ROS/MS and DFI were associated with clinical outcomes in ART.

Total reactive antioxidant potential and DNA fragmentation index as fertility sperm parameters

2011 ◽  
Vol 11 (2) ◽  
pp. 135-144 ◽  
Author(s):  
Piotr Miciński ◽  
Krzysztof Pawlicki ◽  
Ewa Wielgus ◽  
Michał Bochenek ◽  
Piotr Gogol ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Antioxidant Potential ◽  
Sperm Parameters ◽  
Fragmentation Index ◽  
Dna Fragmentation Index
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