RNA-Seq analysis of resistant and susceptible potato varieties during the early stages of potato virus Y infection

Virus elimination in potato through meristem culture followed by thermotherapy and virus indexing was studied. Three levels of thermotherapy, viz. 27±1°C (control), 30±1°C and 35±1°C, sixteen combinations of BAP (Benzyl Amino Purine) plus GA3 (gibberellic Acid) concentrations viz. 0.0+0.0 (control), 0.0+0.2, 0.0+0.4, 0.0+0.6, 1.5+0.0, 1.5+0.2, 1.5+0.4, 1.5+0.6, 3.0+0.0, 3.0+0.2, 3.0+0.4, 3.0+0.6 , 4.5+0.0, 4.5+ 0.2, 4.5+0.4 and 4.5+0.6 were used in this study in three potato varieties viz. Diamant, Heera and Lalpakri. Among the thermo therapies, 27±1°C showed the highest (24.55) survival response followed by 30±1°C, 35±1°C, respectively. The poorest (20.47) survival response of meristem derived plantlets was noticed in 35±1°C which gave the highest percentage (43.79) of virus free plantlets followed by 30±1°C. The best (25.85%) survival response was found in Lalpakri and the lowest (19.08%) survivality was recorded in Diamant. The highest (33.27) percentage of PVY (Potato Virus Y) free plantlets was observed in Heera. The combined treatment 3.0 mg L-1 BAP and 0.2 mg L-1 GA3 showed the highest (63.39) percentage of virus free plantlet production followed by 4.5 mg L-1 BAP and 0.2 mg L-1 GA3DOI: http://dx.doi.org/10.3329/sja.v11i1.18376 SAARC J. Agri., 11(1): 71-80 (2013)

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The effect of potato rugose on the yield of some potato varieties

Agricultural and Food Science ◽

10.23986/afsci.71567 ◽

1961 ◽

Vol 33 (1) ◽

pp. 89-100

Author(s):

Onni Pohjakallio ◽

Laura Karhuvaara ◽

Simo Antila

Keyword(s):

Virus Infection ◽

Potato Virus ◽

Potato Plant ◽

Potato Virus Y ◽

Tuber Yield ◽

Virus Disease ◽

Potato Varieties ◽

Potato Plants ◽

Severe Type

The virus disease occurring in the potato varieties Harbinger, Aquila, and King George V was probably the potato rugose caused by potato virus Y (Solanum virus 2). In the variety Harbinger, the degree of strength of the virus disease probably varied as a result of the appearance of different races of potato virus Y. The type of the virus disease did not change from one vegetative generation of the potato to another. In the potato varieties Aquila and King George V, only the severe type of the disease was observed. The severe type of the virus disease reduced the tuber yield of all the potato varieties investigated to a level of 5 to 6 %, and the slight type occurring in the variety Harbinger to an average of 27 % of the normal. The virus infection appeared more frequently in Harbinger than in the variety Aquila. In the summer, when the infection of the potato plant took place, the virus disease reduced its productivity only relatively little. In the following vegetative generation of the diseased potato plants, the degree of their degeneration conclusively displayed itself. Following this, the wakening of the virus diseased plants did not progress further from one vegetative generation to the next.

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First results of assessment potato varieties of Kazakhstan breeding on the availability of resistance genes to potato virus Y with the help of molecular markers

Agrarian science ◽

10.32634/0869-8155-2019-326-3-23-27 ◽

2019 ◽

Vol 326 (3) ◽

pp. 23-27

Author(s):

B. Beisembina ◽

◽

O.A. Kuzminova ◽

A.S. Udovitsky ◽

K.M. Musynov ◽

...

Keyword(s):

Molecular Markers ◽

Potato Virus ◽

Resistance Genes ◽

Potato Virus Y ◽

Potato Varieties ◽

First Results

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Foliar and Tuber Symptoms of U.S. Potato Varieties to Multiple Strains and Isolates of Potato virus Y

American Journal of Potato Research ◽

10.1007/s12230-020-09820-1 ◽

2021 ◽

Author(s):

Jonathan L. Whitworth ◽

Stewart M. Gray ◽

Jason T. Ingram ◽

Darren G. Hall

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Potato Varieties ◽

Multiple Strains

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THE REACTIONS OF CERTAIN SOLANACEOUS SPECIES TO STRAINS OF POTATO VIRUS Y

Canadian Journal of Botany ◽

10.1139/b55-031 ◽

1955 ◽

Vol 33 (4) ◽

pp. 355-361 ◽

Cited By ~ 14

Author(s):

James Munro

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Avirulent Strain ◽

Potato Varieties ◽

Nicotiana Species ◽

Solanaceous Species ◽

Leaf Drop ◽

Young Leaves

Strains of potato virus Y that: were not clearly differentiated from each other by their reaction on potato varieties were shown to be separate entities by the differing reactions that they caused in several other solanaceous species. One strain did not infect Physalis floridana, but this same strain caused necrosis and a severe distortion of the young leaves of Nicotiana sanderae. After infection with certain other strains, N. rustica showed leaf-drop streak symptoms; this observation has not been previously reported. An avirulent strain in all potato varieties and seedlings tested so far caused a severely necrotic disease in N. tabacum and other Nicotiana species.

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Field Evaluation and Enzyme-Linked Immunosorbent Assay Detection of Potato Leaf Roll Virus, Potato Virus X and Potato Virus Y in Potato Germplasm

Journal of Agricultural Science ◽

10.5539/jas.v9n7p229 ◽

2017 ◽

Vol 9 (7) ◽

pp. 229 ◽

Cited By ~ 1

Author(s):

Romana Anjum ◽

M. Aslam Khan ◽

Kolawole Oluwaseun Olawale ◽

Raheel Baber

Keyword(s):

Immunosorbent Assay ◽

Potato Virus ◽

Potato Virus Y ◽

Rating Scale ◽

Leaf Roll ◽

Potato Virus X ◽

Potato Leaf Roll Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Potato Leaf ◽

Potato Varieties

Polerovirus: potato leaf roll virus (PLRV), Potyvirus: potato virus Y (PVY) and Potexvirus: potato virus X (PVX) is more destructive and well distributed throughout the Pakistan. Incidence has been reported to be as high as 90%, 25%, and ≥ 15%, respectively in the potato growing regions. To find out the source of resistance, twenty-nine virus free potato varieties were grown under field conditions with good agricultural practices. The disease severity of PLRV, PVY and PVX was recorded to determine the level of resistance of the potato varieties according to the disease rating scale. Infectivity and biological assay of all twenty-nine varieties were done in green house on potato, Datura stramonium, Nicotiana glutinosa and Physalis floridana. Non-inoculated plants were served as control. Leaf samples from potato varieties were collected for serological detection of PLRV, PVY and PVX by Double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). Out of twenty nine varieties, none of the variety was resistant to PLRV although three varieties; Mirrato, 394021-120 and Orla were moderately resistant. Only FD 48-4 and TPS 9813 showed resistance to PVX and PVY. While FD 3-10, FD 9616 and FD 37-13 were moderately to PVX and PVY. Rest of the varieties was found susceptible to all three viruses.

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