Proteomic analysis of the rice (Oryza officinalis) provides clues on molecular tagging of proteins for brown planthopper resistance

Restriction fragment length polymorphism analysis was carried out to tag the alien genes for brown planthopper (BPH) resistance and earliness introgressed from wild species Oryza australiensis into cultivated rice, O. sativa L. One introgression line (IR65482-4-136-2-2), resistant to biotypes 1, 2, and 3 of BPH and early in flowering, was selected from BC2F4 of the cross between O. sativa (IR31917-45-3-2) and O. australiensis (accession 100882). Recurrent parent, O. australiensis, and introgression line were surveyed for RFLP using probes of chromosomes 10 and 12. Two probes, RG457 and CDO98, detected introgression from O. australiensis. Cosegregation between introgressed characters and molecular markers was studied in F2 derived from the cross between the introgression line and recurrent parent. The gene for BPH resistance is linked with RG457 of chromosome 12 at a distance of 3.68 ± 1.29 cM, and the gene for earliness is linked with CDO98 of chromosome 10 at a distance of 9.96 ± 3.28 cM. Such close linkage is useful in marker-based selection while transferring BPH resistance from introgression line into other elite breeding lines. Introgression at the molecular level indicates that the mechanism of alien gene transfer is probably genetic recombination through crossing over rather than substitution of whole or large segment of chromosomes of wild species.Key words: molecular tagging, alien gene introgression, rice, wild species, brown planthopper resistance.

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Small brown planthopper resistance loci in wild rice (Oryza officinalis)

Molecular Genetics and Genomics ◽

10.1007/s00438-014-0814-8 ◽

2014 ◽

Vol 289 (3) ◽

pp. 373-382 ◽

Cited By ~ 14

Author(s):

Weilin Zhang ◽

Yan Dong ◽

Ling Yang ◽

Bojun Ma ◽

Rongrong Ma ◽

...

Keyword(s):

Wild Rice ◽

Brown Planthopper ◽

Oryza Officinalis ◽

Small Brown Planthopper ◽

Brown Planthopper Resistance

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Analysis of QTLs for Brown Planthopper Resistance inIndicaRice WD15515

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2016.00353 ◽

2016 ◽

Vol 42 (3) ◽

pp. 353 ◽

Cited By ~ 1

Author(s):

Zhao DENG ◽

Shao-Jie SHI ◽

Hui-Ying WANG ◽

Xin-Xin SHANG-GUAN ◽

Bing-Fang LIU ◽

...

Keyword(s):

Brown Planthopper ◽

Brown Planthopper Resistance

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The distribution and identification of brown planthopper resistance genes in rice

Hereditas ◽

10.1111/j.1601-5223.2009.02088.x ◽

2009 ◽

Vol 146 (2) ◽

pp. 67-73 ◽

Cited By ~ 14

Author(s):

Yuqiang Liu ◽

Changchao Su ◽

Ling Jiang ◽

Jun He ◽

Han Wu ◽

...

Keyword(s):

Resistance Genes ◽

Brown Planthopper ◽

Brown Planthopper Resistance

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High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds

Theoretical and Applied Genetics ◽

10.1007/s00122-010-1413-7 ◽

2010 ◽

Vol 121 (8) ◽

pp. 1601-1611 ◽

Cited By ~ 59

Author(s):

Yongfu Qiu ◽

Jianping Guo ◽

Shengli Jing ◽

Lili Zhu ◽

Guangcun He

Keyword(s):

High Resolution ◽

Resistance Gene ◽

Brown Planthopper ◽

High Resolution Mapping ◽

Resolution Mapping ◽

Brown Planthopper Resistance

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Pengujian Kemurnian Genetik Benih Padi Galur F3 (Pandanwangi x PTB33) Terseleksi Menggunakan Marka Molekuler Simple Sequence Repeats (SSR)

Agrikultura ◽

10.24198/agrikultura.v26i2.8462 ◽

2015 ◽

Vol 26 (2) ◽

Author(s):

Syindy Raffini Nasihin ◽

Wieny H. Rizky ◽

Nono Carsono

Keyword(s):

Simple Sequence Repeats ◽

Simple Sequence Repeat ◽

Brown Planthopper ◽

Plant Analysis ◽

Current Experiment ◽

Genetic Purity ◽

Experimental Station ◽

Purity Testing ◽

Brown Planthopper Resistance ◽

Simple Sequence

ABSTRACTSeed Purity Testing of F3 Progeny of Rice Lines Derived from a Cross between Pandanwangi x PTB-33 Estimated by Simple Sequence Repeat MarkersSeeds with high purity is required to produce maximum crop yield. Genetic purity of selected F3rice seed progenies derived from a cross between Pandanwangi x PTB33 was estimated by SSR(simple sequence repeats) molecular markers. Objective of current experiment was to obtain riceseed with high genetic purity (100%) in terms of homogeneity of alleles. The experiment wasconducted at Laboratory of Plant Analysis and Biotechnology, meanwhile field experiment wasperformed at Ciparanje Experimental Station, Faculty of Agriculture, Universitas Padjadjaran.Based on primer designed by Bradbury (aromatic trait) and primers RM589 and RM586 (supposedto correlate with brown planthopper resistance gene), seeds of F3 selected had 100% geneticpurity. SSR markers applied for each offspring were able to demonstrate the purity of the seed.Genotypes with 100% genetic purity can be continued for propagating their seeds.Keywords: F3, seed purity, seed rice, SSR markersABSTRAKBenih dengan kemurnian genetik tinggi sangat diperlukan untuk produksi tanaman yangmaksimal. Kemurnian genetik padi generasi F3 hasil persilangan Pandanwangi x PTB-33 diestimasidengan menggunakan marka molekuler SSR. Percobaan ini bertujuan untuk mendapatkan generasiF3 yang memiliki kemurnian genetik 100%. Penelitian dilaksanakan di Kebun PercobaanCiparanje, Fakultas Pertanian Universitas Padjadjaran dan Laboratorium Analisis dan BioteknologiTanaman. Hasil analisis menggunakan primer Bradbury menunjukkan 100% benih murniberdasarkan karakter aromatik, begitupun berdasarkan karakter ketahanan terhadap wereng(primer RM589 dan RM586) menunjukkan 100% benih murni. Marka molekuler SSR yangdigunakan untuk verifikasi kemurnian mampu menunjukkan kemurnian genetik benih padi yangtinggi. Genotip PP dapat dilanjutkan untuk pengujian dan atau perbanyakan benih sumber.Kata kunci: benih padi, F3, marka SSR, uji kemurnian genetik

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LOW ASSOCIATION OF Bph17 ALLELE IN LANDRACES AND IMPROVED VARIETIES OF RICE RESISTANT TO BROWN PLANTHOPPER

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v18n1.2017.p1-6 ◽

2017 ◽

Vol 18 (1) ◽

pp. 1

Author(s):

Wage Ratna Rohaeni ◽

Untung Susanto ◽

Aida F.V. Yuningsih

Keyword(s):

Brown Planthopper ◽

Gene Marker ◽

Specific Gene ◽

Rice Varieties ◽

Improved Varieties ◽

Recessive Genes ◽

Check Variety ◽

Rice Genotypes ◽

Brown Planthopper Resistance ◽

Dominant Genes

<p>Resistance traits to brown planthopper on rice varieties are controlled by dominant and recessive genes called Bph/bph. Bph17 is one of dominant genes that control rice resistance to brown planthopper. Marker of Bph17 allele can be used as a tool of marker assisted selection (MAS) in breeding activity. Association of Bph17 allele and resistance to brown planthopper in Indonesian landraces and new-improved varieties of rice is not clearly known. The study aimed to determine the association of Bph17 allele in landraces and new-improved varieties of rice resistant to brown planthopper. Twenty-one rice genotypes were used in the study, consisting of 13 landraces, 5 improved varieties, 3 popular varieties and a check variety Rathu Heenati. Two simple sequence repeat markers linked to Bph17 allele were used, i.e. RM8213 and RM5953. The results showed that association of Bph17 allele in landraces and new-improved varieties of rice resistant to brown planthopper resistance was very low (r = -0.019 and -0.023, respectively). The presence of Bph17 allele did not constantly express resistance to brown planthopper. The study suggests that Bph17 allele cannot be used as a tool of MAS for evaluating resistance of landraces and new-improved varieties of rice to brown planthopper. Further research is needed to obtain a specific gene marker that can be used as a tool of MAS and applicable for Indonesian differential rice varieties. </p>

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