scholarly journals LOW ASSOCIATION OF Bph17 ALLELE IN LANDRACES AND IMPROVED VARIETIES OF RICE RESISTANT TO BROWN PLANTHOPPER

2017 ◽  
Vol 18 (1) ◽  
pp. 1
Author(s):  
Wage Ratna Rohaeni ◽  
Untung Susanto ◽  
Aida F.V. Yuningsih
Keyword(s):  
Brown Planthopper ◽  
Gene Marker ◽  
Specific Gene ◽  
Rice Varieties ◽  
Improved Varieties ◽  
Recessive Genes ◽  
Check Variety ◽  
Rice Genotypes ◽  
Brown Planthopper Resistance ◽  
Dominant Genes

<p>Resistance traits to brown planthopper on rice varieties are controlled by dominant and recessive genes called Bph/bph. Bph17 is one of dominant genes that control rice resistance to brown planthopper.  Marker of Bph17 allele can be used as a tool of marker assisted selection (MAS) in breeding activity. Association of Bph17 allele and resistance to brown planthopper in Indonesian landraces and new-improved varieties of rice is not clearly known. The study aimed to determine the association of Bph17 allele in landraces and new-improved varieties of rice resistant to brown planthopper. Twenty-one rice genotypes were used in the study, consisting of 13 landraces, 5 improved varieties, 3 popular varieties and a check variety Rathu Heenati. Two simple sequence repeat markers linked to Bph17 allele were used, i.e. RM8213 and RM5953. The results showed that association of Bph17 allele in landraces and new-improved varieties of rice resistant to brown planthopper resistance was very low (r = -0.019 and -0.023, respectively). The presence of Bph17 allele did not constantly express resistance to brown planthopper. The study suggests that Bph17 allele cannot be used as a tool of MAS for evaluating resistance of landraces and new-improved varieties of rice to brown planthopper. Further research is needed to obtain a specific gene marker that can be used as a tool of MAS and applicable for Indonesian differential rice varieties. </p>

scholarly journals IDENTIFICATION OF POLYMORPHISM ON SIMPLE SEQUENCE REPEATS MARKERS ASSOCIATED WITH BROWN PLANTHOPPER RESISTANCE GENES IN TWENTY RICE GENOTYPES AND THEIR GENETIC RELATIONSHIP

2015 ◽  
Vol 2 (1) ◽  
pp. 98
Author(s):  
Nono Carsono ◽  
Yessikha V. Barus ◽  
Santika Sari ◽  
Winny D. Widarmi ◽  
Danar Dono ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Resistance Genes ◽  
Genetic Relationship ◽  
Polymorphic Information Content ◽  
Durable Resistance ◽  
Brown Planthopper ◽  
Bph Genes ◽  
Rice Genotypes ◽  
Brown Planthopper Resistance ◽  
Simple Sequence

<p>Brown planthopper is one of the most destructive insect pest of rice in Indonesia and other Asian countries. Pyramiding some brown planthopper resistance genes is a valuable approach to create more durable resistance against the pest. The objective of this study was to identify polymorphisms of Brown Planthopper Resistance genes (Bph) on 20 genotypes of rice, and to obtain genetic relationship among genotypes tested. The experiment was conducted from June to September 2012 at Green House and Laboratory of Plant Analysis and Biotechnology, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor. Twenty genotypes were analyzed, and two of them were used as check varieties. Simple Sequence Repeat (SSR) markers were applied to detect Bph3, Bph4, Qbph3, and Qbph4 genes. Polymorphic levels were analyzed by calculating PIC (Polymorphic Information Content). The grouping of rice genotypes were done based on principal components analysis (PCA) of SSR data, and the genetic relationship based on the presence of Bph genes was estimated using UPGMA (Unweighted Pair Group With Arithmetic mean). Results showed that RM313, RM8072, RM8213, RM5953, RM586, and RM589 markers were polymorphic. Rice genotypes PTB 33, Diah Suci, Cibogo, Cisantana, Digul, Ciherang, Inpari 13, Inpari 10, and Memberamo had Bph3, Qbph3, Bph4, and Qbph4. Meanwhile Bph3, Qbph3, and Bph4 were supposed to be belonged by IR 64, Aek Sibundong, Batang Gadis, IR 66, and Mekongga. Kalimas and Tukat Penatu had Bph3, Qbph3, dan Qbph4. IR 74 had Bph3 and Qbph3, and Fatmawati had Bph3 and Bph4. UPGMA clustering resulted in two main clusters, in which the first cluster consisted of 2 subclusters. PTB-33 was closely related with Memberamo, Tukat Penatu, Digul, Diah Suci, and Kalimas. The SSR markers used in this study were proven to be valuable in molecular detection of Bph genes and in estimating genetic relationsips of rice genotypes. PTB-33 was a good donor of resistance genes, as well as Memberamo, Tukat Penatu, Digul, Diah Suci, and Kalimas which were identified as promising donors in rice breeding resistance to brown planthopper. </p><p><strong>Keywords :</strong> Bph gene, Brown Planthopper, Genetic relationship, SSR markers.</p>

scholarly journals Identification of two QTLs, BPH41 and BPH42, and their respective gene candidates for brown planthopper resistance in rice by a combinatorial approach of QTL mapping and RNA-seq

2021 ◽  
Author(s):  
Han Qi Tan ◽  
Sreekanth Palyam ◽  
Jagadeesha Gouda ◽  
Prakash Kumar ◽  
Santhosh Kumar Chellian
Keyword(s):  
Innate Immunity ◽  
Brown Planthopper ◽  
Resistance Mechanism ◽  
Rna Seq ◽  
Rice Varieties ◽  
Major Pest ◽  
Differential Gene ◽  
Brown Planthopper Resistance ◽  
Innate Immunity Pathway ◽  
Successful Production

Abstract The brown planthopper (BPH) is the leading cause of insect damage to rice plants. It has caused profound losses in rice production since the late 1970’s and continues to be a major pest problem. Therefore, there is an urgent need to discover new BPH resistance genes to ensure the successful production of rice. Here, a new BPH resistance source provided by SeedWorks International Pvt. Ltd., SWD10, was used for this purpose. A total of 232 F2 progenies were genotyped with 216 polymorphic markers. Results revealed two dominant QTLs located on the short and long arms of chromosome 4. These QTLs are referred to as BPH41 and BPH42, respectively. BPH resistance mechanism test revealed that antibiosis and antixenosis mechanisms both play a role in BPH resistance conferred by these two QTLs. To further reduce the size of each QTL, 14,368 recombinant lines were screened, and the QTLs were delimited between markers SWRm_01617 and SWRm_01522 for BPH41, and SWRm_01695 and SWRm_00328 for BPH42. Additionally, using RNA-seq data of lines containing the QTLs, we further shortlisted four and three gene candidates for BPH41 and BPH42, respectively. Differential gene expression analysis of lines containing the QTLs suggested that SWD10 BPH resistance is contributed by the plant’s innate immunity and the candidate genes may be part of the rice innate immunity pathway. The newly identified QTLs are currently being utilized for breeding BPH resistant rice varieties and hybrids.

scholarly journals Rice Sesquiterpene Plays Important Roles in Antixenosis against Brown Planthopper in Rice

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1049
Author(s):  
Wintai Kamolsukyeunyong ◽  
Wissarut Sukhaket ◽  
Kitsada Pitija ◽  
Pornwalai Thorngkham ◽  
Sugunya Mahatheeranont ◽  
...  
Keyword(s):  
Amino Acid ◽  
Sequence Analysis ◽  
Promoter Region ◽  
Defense Mechanism ◽  
Rice Variety ◽  
Brown Planthopper ◽  
Major Product ◽  
Amino Acid Deletion ◽  
Rice Varieties ◽  
Rice Genotypes

The rice sesquiterpene synthase II gene (OsSTPS2, LOC_Os04g27430), which is involved in the antixenosis defense mechanism of rice against brown planthopper (BPH) infestation, was identified in the BPH-resistant rice variety Rathu Heenati (RH). In contrast, the gene was not functional in the BPH-susceptible rice variety KDML105 (KD). Single-nucleotide polymorphisms (SNPs) in the promoter region and in exon 5 of the gene and a seven amino acid deletion in the deduced protein sequence are suggested as factors that negatively regulate the function of the gene. Sequence analysis of the promoter region and expression analysis of the OsSTPS2 gene in several rice genotypes revealed the correlation of SNPs of the ATHB-1, SBE1, and P-factor with the expression of the gene. Genomic and complementary DNA (cDNA) sequence analysis at exon 5 of the gene showed that the 21 bp deletion naturally occurred in several rice genotypes. The antixenosis of the BPH feeding preference (AFP) of rice varieties differed in the seven amino acid deletion lesion of the gene, suggesting that the seven amino acid deletion negatively controls the antixenosis mechanism during BPH infestation. Analysis of the plant volatile compounds released after BPH infestation suggested that E-β-farnesene (EBF) is the major product of the OsSTPS2 gene.

Identification of antibiosis and tolerance in rice varieties carrying brown planthopper resistance genes

2011 ◽  
Vol 141 (3) ◽  
pp. 224-231 ◽  
Author(s):  
Yongfu Qiu ◽  
Jianping Guo ◽  
Shengli Jing ◽  
Ming Tang ◽  
Lili Zhu ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Brown Planthopper ◽  
Rice Varieties ◽  
Brown Planthopper Resistance

scholarly journals Screening of Advanced Aromatic Rice Lines Using Morphological and Physico-Chemical Characteristics

2020 ◽  
Vol 22 (2) ◽  
pp. 91-102
Author(s):  
S Paul ◽  
PK Biswas ◽  
MS Islam ◽  
SS Siddique ◽  
BJ Shirazy ◽  
...  
Keyword(s):  
Grain Yield ◽  
Plant Height ◽  
Grain Shape ◽  
Aromatic Rice ◽  
Rice Varieties ◽  
Rice Plants ◽  
Physico Chemical ◽  
Kernel Length ◽  
Check Variety ◽  
Rice Genotypes

Short-statured and lodging resistant rice plants with long to medium slender grain are the expected criteria for aromatic rice. However, most of the aromatic rice varieties in Bangladesh do not meet the expected criteria. Therefore, this study was undertaken to detect short-statured rice plants with aromatic and long to medium slender grain where twelve advanced rice lines (derived from the local rice germplasm) with a local check Kataribhog were evaluated. All the genotypes demonstrated significant variation for different parameters such as plant height at harvest, yield per hectare, grain length, grain size, grain shape, and aroma. At harvest, the shortest plant height was obtained from SAU ADL11 (107.09cm), and it was statistically similar to SAU ADL4 (111.68cm) and SAU ADL5 (111.89cm). However, the plant height of check variety Kataribhog was 167.17cm. The highest grain yield per hectare was found in SAU ADL5 (4.79 tha-1), where it was 2.71 tha-1 in Kataribhog. Besides, the grain yield of shorter genotypes SAU ADL4 and SAU ADL11 was 3.47 t ha-1 and 3.84 t ha-1 , respectively. The SAU ADL1 provided the highest kernel length (7.31mm), and the lowest kernel length (4.87 mm) was recorded in the check. Kernel length of short-statured genotypes ranged from 7.01 mm to 6.57 mm. Kernel size of all evaluated genotypes was long to medium; whereas, it was short for Kataribhog. Kernel shape of these short-statured genotypes was slender to medium. In case of aroma, SAU ADL3, SAU ADL5, SAU ADL7, SAU ADL9, SAU ADL10, and SAU ADL11 were moderately aromatic, and other genotypes were non-aromatic. However, Kataribhog was strongly aromatic. Finally, SAU ADL5 and SAU ADL11 were evaluated as important germplasms in respect of different characters such as shortstatured plant, long to medium slender grain, and aroma. These two lines could be potential inbreed aromatic rice genotypes for Bangladesh. Bangladesh Agron. J. 2019, 22(2): 91-102

Screening of rice genotypes for resistance to the brown planthopper, Nilaparvata lugens Stål

2012 ◽  
Vol 40 (4) ◽  
pp. 502-508 ◽  
Author(s):  
M. P. Ali ◽  
Salem S. Alghamdi ◽  
M. A. Begum ◽  
A. B. M. Anwar Uddin ◽  
M. Z. Alam ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Genotypes

Analysis of QTLs for Brown Planthopper Resistance inIndicaRice WD15515

2016 ◽  
Vol 42 (3) ◽  
pp. 353 ◽  
Author(s):  
Zhao DENG ◽  
Shao-Jie SHI ◽  
Hui-Ying WANG ◽  
Xin-Xin SHANG-GUAN ◽  
Bing-Fang LIU ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Brown Planthopper Resistance

scholarly journals The distribution and identification of brown planthopper resistance genes in rice

Hereditas ◽  
2009 ◽  
Vol 146 (2) ◽  
pp. 67-73 ◽  
Author(s):  
Yuqiang Liu ◽  
Changchao Su ◽  
Ling Jiang ◽  
Jun He ◽  
Han Wu ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Brown Planthopper ◽  
Brown Planthopper Resistance
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