The role of protein-protein interactions mediated by the PB1 domain of NLP transcription factors in nitrate-inducible gene expression

ABSTRACT Hypertrophic growth of cardiac muscle cells is induced by a variety of physiological and pathological stimuli and is associated with a number of changes, including activation of genes such as atrial natriuretic factor. We found that two serum response element (SRE)-like DNA elements, one of which does not meet the consensus sequence and binds serum response factor (SRF) with low affinity, regulate the activity of this promoter. Surprisingly, the ability to induce the promoter by two different physiologic stimuli, as well as various activated transcription factors, including SRF-VP16, was primarily dependent upon the nonconsensus rather than the consensus SRE. This SRE controls the induction of gene expression via an unusual mechanism in that it is required to allow some, but not all, active transcription factors at unrelated sites on the promoter to stimulate gene expression. Thus, in addition to regulation of SRF activity by growth stimuli, regulation of a low-affinity SRE element controls inducible gene expression by modulating the ability of other transcription factors to stimulate the transcription machinery.

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A differential role of CREB phosphorylation in cAMP-inducible gene expression in the rat pineal

Brain Research ◽

10.1016/s0006-8993(00)02185-5 ◽

2000 ◽

Vol 864 (2) ◽

pp. 270-280 ◽

Cited By ~ 11

Author(s):

Rainer Spessert ◽

Maria Rapp ◽

Holger Jastrow ◽

Nesrin Karabul ◽

Frank Blum ◽

...

Keyword(s):

Gene Expression ◽

Inducible Gene Expression ◽

Creb Phosphorylation ◽

Inducible Gene

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Transcription factors of the NF1 family: Possible mechanisms of inducible gene expression in the evolutionary lineage of multicellular animals

Journal of Evolutionary Biochemistry and Physiology ◽

10.1134/s123456781702001x ◽

2017 ◽

Vol 53 (2) ◽

pp. 85-92 ◽

Cited By ~ 2

Author(s):

E. V. Romanovskaya ◽

M. V. Vikhnina ◽

T. V. Grishina ◽

M. P. Ivanov ◽

L. E. Leonova ◽

...

Keyword(s):

Gene Expression ◽

Transcription Factors ◽

Inducible Gene Expression ◽

Evolutionary Lineage ◽

Inducible Gene

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Mechanism of gene expression by the glucocorticoid receptor: Role of protein-protein interactions

BioEssays ◽

10.1002/bies.950190210 ◽

1997 ◽

Vol 19 (2) ◽

pp. 153-160 ◽

Cited By ~ 134

Author(s):

Iain J. McEwan ◽

Anthony P. H. Wright ◽

Jan-�ke Gustafsson

Keyword(s):

Gene Expression ◽

Glucocorticoid Receptor ◽

Protein Interactions ◽

Protein Protein Interactions

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Posttranslational regulation of multiple clock-related transcription factors triggers cold-inducible gene expression in Arabidopsis

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.2021048118 ◽

2021 ◽

Vol 118 (10) ◽

pp. e2021048118

Author(s):

Satoshi Kidokoro ◽

Kentaro Hayashi ◽

Hiroki Haraguchi ◽

Tomona Ishikawa ◽

Fumiyuki Soma ◽

...

Keyword(s):

Gene Expression ◽

Transcription Factors ◽

Cold Stress ◽

Circadian Clock ◽

Crop Productivity ◽

Stress Conditions ◽

Posttranslational Regulation ◽

Inducible Gene Expression ◽

Myb Proteins ◽

Inducible Gene

Cold stress is an adverse environmental condition that affects plant growth, development, and crop productivity. Under cold stress conditions, the expression of numerous genes that function in the stress response and tolerance is induced in various plant species, and the dehydration-responsive element (DRE) binding protein 1/C-repeat binding factor (DREB1/CBF) transcription factors function as master switches for cold-inducible gene expression. Cold stress strongly induces these DREB1 genes. Therefore, it is important to elucidate the mechanisms of DREB1 expression in response to cold stress to clarify the perception and response of cold stress in plants. Previous studies indicated that the central oscillator components of the circadian clock, CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY), are involved in cold-inducible DREB1 expression, but the underlying mechanisms are not clear. We revealed that the clock-related MYB proteins REVEILLE4/LHY-CCA1-Like1 (RVE4/LCL1) and RVE8/LCL5 are quickly and reversibly transferred from the cytoplasm to the nucleus under cold stress conditions and function as direct transcriptional activators of DREB1 expression. We found that CCA1 and LHY suppressed the expression of DREB1s under unstressed conditions and were rapidly degraded specifically in response to cold stress, which suggests that they act as transcriptional repressors and indirectly regulate the cold-inducible expression of DREB1s. We concluded that posttranslational regulation of multiple clock-related transcription factors triggers cold-inducible gene expression. Our findings clarify the complex relationship between the plant circadian clock and the regulatory mechanisms of cold-inducible gene expression.

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Faculty Opinions recommendation of Posttranslational regulation of multiple clock-related transcription factors triggers cold-inducible gene expression in Arabidopsis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.739665053.793583716 ◽

2021 ◽

Author(s):

Ramón Serrano

Keyword(s):

Gene Expression ◽

Transcription Factors ◽

Posttranslational Regulation ◽

Inducible Gene Expression ◽

Inducible Gene

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Radiosensitive and Mitotic Recombination Phenotypes of the Saccharomyces cerevisiae dun1 Mutant Defective in DNA Damage-Inducible Gene Expression

Genetics ◽

10.1093/genetics/152.3.909 ◽

1999 ◽

Vol 152 (3) ◽

pp. 909-919 ◽

Cited By ~ 1

Author(s):

Michael Fasullo ◽

Joseph Koudelik ◽

Peter AhChing ◽

Peter Giallanza ◽

Cinzia Cera

Keyword(s):

Gene Expression ◽

Saccharomyces Cerevisiae ◽

Dna Damage ◽

Biological Significance ◽

Uv Damage ◽

Triple Mutant ◽

Inducible Gene Expression ◽

Dna Damaging Agents ◽

Inducible Gene

Abstract The biological significance of DNA damage-induced gene expression in conferring resistance to DNA-damaging agents is unclear. We investigated the role of DUN1-mediated, DNA damage-inducible gene expression in conferring radiation resistance in Saccharomyces cerevisiae. The DUN1 gene was assigned to the RAD3 epistasis group by quantitating the radiation sensitivities of dun1, rad52, rad1, rad9, rad18 single and double mutants, and of the dun1 rad9 rad52 triple mutant. The dun1 and rad52 single mutants were similar in terms of UV sensitivities; however, the dun1 rad52 double mutant exhibited a synergistic decrease in UV resistance. Both spontaneous intrachromosomal and heteroallelic gene conversion events between two ade2 alleles were enhanced in dun1 mutants, compared to DUN1 strains, and elevated recombination was dependent on RAD52 but not RAD1 gene function. Spontaneous sister chromatid exchange (SCE), as monitored between truncated his3 fragments, was not enhanced in dun1 mutants, but UV-induced SCE and heteroallelic recombination were enhanced. Ionizing radiation and methyl methanesulfonate (MMS)-induced DNA damage did not exhibit greater recombinogenicity in the dun1 mutant compared to the DUN1 strain. We suggest that one function of DUN1-mediated DNA damage-induced gene expression is to channel the repair of UV damage into a nonrecombinogenic repair pathway.

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