Faculty Opinions recommendation of Posttranslational regulation of multiple clock-related transcription factors triggers cold-inducible gene expression in Arabidopsis.

2021 ◽  
Author(s):  
Ramón Serrano
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Posttranslational Regulation ◽  
Inducible Gene Expression ◽  
Inducible Gene

scholarly journals Posttranslational regulation of multiple clock-related transcription factors triggers cold-inducible gene expression in Arabidopsis

2021 ◽  
Vol 118 (10) ◽  
pp. e2021048118
Author(s):  
Satoshi Kidokoro ◽  
Kentaro Hayashi ◽  
Hiroki Haraguchi ◽  
Tomona Ishikawa ◽  
Fumiyuki Soma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Cold Stress ◽  
Circadian Clock ◽  
Crop Productivity ◽  
Stress Conditions ◽  
Posttranslational Regulation ◽  
Inducible Gene Expression ◽  
Myb Proteins ◽  
Inducible Gene

Cold stress is an adverse environmental condition that affects plant growth, development, and crop productivity. Under cold stress conditions, the expression of numerous genes that function in the stress response and tolerance is induced in various plant species, and the dehydration-responsive element (DRE) binding protein 1/C-repeat binding factor (DREB1/CBF) transcription factors function as master switches for cold-inducible gene expression. Cold stress strongly induces these DREB1 genes. Therefore, it is important to elucidate the mechanisms of DREB1 expression in response to cold stress to clarify the perception and response of cold stress in plants. Previous studies indicated that the central oscillator components of the circadian clock, CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY), are involved in cold-inducible DREB1 expression, but the underlying mechanisms are not clear. We revealed that the clock-related MYB proteins REVEILLE4/LHY-CCA1-Like1 (RVE4/LCL1) and RVE8/LCL5 are quickly and reversibly transferred from the cytoplasm to the nucleus under cold stress conditions and function as direct transcriptional activators of DREB1 expression. We found that CCA1 and LHY suppressed the expression of DREB1s under unstressed conditions and were rapidly degraded specifically in response to cold stress, which suggests that they act as transcriptional repressors and indirectly regulate the cold-inducible expression of DREB1s. We concluded that posttranslational regulation of multiple clock-related transcription factors triggers cold-inducible gene expression. Our findings clarify the complex relationship between the plant circadian clock and the regulatory mechanisms of cold-inducible gene expression.

scholarly journals A Low-Affinity Serum Response Element Allows Other Transcription Factors To Activate Inducible Gene Expression in Cardiac Myocytes

1999 ◽  
Vol 19 (3) ◽  
pp. 1841-1852 ◽  
Author(s):  
Wirt A. Hines ◽  
Jacqueline Thorburn ◽  
Andrew Thorburn
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Consensus Sequence ◽  
Serum Response Element ◽  
Cardiac Muscle Cells ◽  
Response Element ◽  
Inducible Gene Expression ◽  
Hypertrophic Growth ◽  
Serum Response ◽  
Inducible Gene

ABSTRACT Hypertrophic growth of cardiac muscle cells is induced by a variety of physiological and pathological stimuli and is associated with a number of changes, including activation of genes such as atrial natriuretic factor. We found that two serum response element (SRE)-like DNA elements, one of which does not meet the consensus sequence and binds serum response factor (SRF) with low affinity, regulate the activity of this promoter. Surprisingly, the ability to induce the promoter by two different physiologic stimuli, as well as various activated transcription factors, including SRF-VP16, was primarily dependent upon the nonconsensus rather than the consensus SRE. This SRE controls the induction of gene expression via an unusual mechanism in that it is required to allow some, but not all, active transcription factors at unrelated sites on the promoter to stimulate gene expression. Thus, in addition to regulation of SRF activity by growth stimuli, regulation of a low-affinity SRE element controls inducible gene expression by modulating the ability of other transcription factors to stimulate the transcription machinery.

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