scholarly journals Functional characterization of a liverworts bHLH transcription factor involved in the regulation of bisbibenzyls and flavonoids biosynthesis

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yu Zhao ◽  
Yu-Ying Zhang ◽  
Hui Liu ◽  
Xiao-Shuang Zhang ◽  
Rong Ni ◽  
...  
Keyword(s):  
Vascular Plants ◽  
Functional Characterization ◽  
Bhlh Transcription Factor ◽  
Structural Genes ◽  
Helix Loop Helix ◽  
Family Protein ◽  
Transient Expression Experiment ◽  
Flavonoids Biosynthesis ◽  
Bhlh Transcription Factors

Abstract Background The basic helix-loop-helix (bHLH) transcription factors (TFs), as one of the largest families of TFs, play important roles in the regulation of many secondary metabolites including flavonoids. Their involvement in flavonoids synthesis is well established in vascular plants, but not as yet in the bryophytes. In liverworts, both bisbibenzyls and flavonoids are derived through the phenylpropanoids pathway and share several upstream enzymes. Results In this study, we cloned and characterized the function of PabHLH1, a bHLH family protein encoded by the liverworts species Plagiochasma appendiculatum. PabHLH1 is phylogenetically related to the IIIf subfamily bHLHs involved in flavonoids biosynthesis. A transient expression experiment showed that PabHLH1 is deposited in the nucleus and cytoplasm, while the yeast one hybrid assay showed that it has transactivational activity. When PabHLH1 was overexpressed in P. appendiculatum thallus, a positive correlation was established between the content of bibenzyls and flavonoids and the transcriptional abundance of corresponding genes involved in the biosynthesis pathway of these compounds. The heterologous expression of PabHLH1 in Arabidopsis thaliana resulted in the activation of flavonoids and anthocyanins synthesis, involving the up-regulation of structural genes acting both early and late in the flavonoids synthesis pathway. The transcription level of PabHLH1 in P. appendiculatum thallus responded positively to stress induced by either exposure to UV radiation or treatment with salicylic acid. Conclusion PabHLH1 was involved in the regulation of the biosynthesis of flavonoids as well as bibenzyls in liverworts and stimulated the accumulation of the flavonols and anthocyanins in Arabidopsis.

scholarly journals Functional Characterization of Basic Helix-Loop-Helix-PAS Type Transcription Factor NXFin Vivo

2008 ◽  
Vol 284 (2) ◽  
pp. 1057-1063 ◽  
Author(s):  
Norihisa Ooe ◽  
Kozo Motonaga ◽  
Kentaro Kobayashi ◽  
Koichi Saito ◽  
Hideo Kaneko
Keyword(s):  
Transcription Factor ◽  
Functional Characterization ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

Identification and functional characterization of two Bcl-2 family protein genes in Zhikong scallop Chlamys farreri

2015 ◽  
Vol 44 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Haigang Qi ◽  
Guoying Miao ◽  
Li Li ◽  
Huayong Que ◽  
Guofan Zhang
Keyword(s):  
Functional Characterization ◽  
Chlamys Farreri ◽  
Zhikong Scallop ◽  
Family Protein

scholarly journals Functional characterization of the transcription regulator WhiB1 from Gordonia sp. IITR100

Microbiology ◽  
2020 ◽  
Vol 166 (12) ◽  
pp. 1181-1190
Author(s):  
Pooja Murarka ◽  
Aditi Keshav ◽  
Bintu Kumar Meena ◽  
Preeti Srivastava
Keyword(s):  
Type Species ◽  
Functional Characterization ◽  
Rhodococcus Erythropolis ◽  
Multiple Sequence ◽  
Content Type ◽  
Transcription Regulator ◽  
Link Type ◽  
Family Protein ◽  
The Family

WhiB is a transcription regulator which has been reported to be involved in the regulation of cell morphogenesis, cell division, antibiotic resistance, stress, etc., in several members of the family Actinomycetes . The present study describes functional characterization of a WhiB family protein, WhiB1 (protein ID: WP_065632651.1), from Gordonia sp. IITR100. We demonstrate that WhiB1 affects chromosome segregation and cell morphology in recombinant Escherichia coli , Gordonia sp. IITR100 as well as in Rhodococcus erythropolis . Multiple sequence alignment suggests that WhiB1 is a conserved protein among members of the family Actinomycetes . It has been reported that overexpression of WhiB1 leads to repression of the biodesulfurization operon in recombinant E. coli , Gordonia sp. IITR100 and R. erythropolis . A WhiB1-mut containing a point mutation Q116A in the DNA binding domain of WhiB1 led to partial alleviation of repression of the biodesulfurization operon. We show for the first time that the WhiB family protein WhiB1 is also involved in repression of the biodesulfurization operon by directly binding to the dsz promoter DNA.

scholarly journals Genome-wide identification and characterization of melon bHLH transcription factors in regulation of fruit development

2020 ◽  
Author(s):  
Selinge Bai ◽  
Chao Tan ◽  
Yunyun Tian ◽  
Ming Ma ◽  
Agula Hasi
Keyword(s):  
Transcription Factor ◽  
Fruit Ripening ◽  
Distribution Patterns ◽  
Fruit Growth ◽  
Bhlh Transcription Factor ◽  
Transcription Factor Family ◽  
Helix Loop Helix ◽  
Bhlh Genes ◽  
Bhlh Transcription Factors ◽  
Intron Distribution

Abstract Background: The basic helix-loop-helix (bHLH) transcription factor family is one of the largest transcription factor families in plants, and plays crucial roles in plant development. Melon is one of an important horticulture plants, and is an attractive model plant for studying fruit ripening. However, the bHLH gene family of melon has not been identified yet, and functions in fruit growth and ripening are seldom researched. Results: In this study, 118 bHLH genes were identified in the genome of melon. Phylogenetic analysis illustrated that these CmbHLHs could be classified into 16 subfamilies. Intron distribution pattern analysis of bHLH domain found 13 intron distribution patterns in CmbHLHs. CmbHLH genes were unevenly distributed on chromosomes 1 to 12 of the melon genome, and five CmbHLH s were tandem repeat on chromosomes 4 and 8. Expression characters of CmbHLH genes were studied using the transcriptome data. Tissue analysis of indicated CmbHLH32 high expressed in female flowers and early fruit growth stage. Transgenic plant lines of overexpression of CmbHLH32 were constructed, and overexpression of CmbHLH32 result in early fruit ripening compared to the wild type fruit. Conclusions: The bHLH transcription factor family was identified and analyzed for the first time in the melon, overexpression of CmbHLH32 will affect the ripening time of melon fruit, these findings laid a theoretical foundation for further study on the role of bHLH family members in the growth and development of melon .

scholarly journals Identification and Functional Characterization of PtoMYB055 Involved in the Regulation of the Lignin Biosynthesis Pathway in Populus tomentosa

2020 ◽  
Vol 21 (14) ◽  
pp. 4857
Author(s):  
Yiming Sun ◽  
Sha Ren ◽  
Shenglong Ye ◽  
Qiaoyan Tian ◽  
Keming Luo
Keyword(s):  
Cell Wall ◽  
Vascular Plants ◽  
Secondary Cell Wall ◽  
Functional Characterization ◽  
Lignin Biosynthesis ◽  
Transcriptional Activator ◽  
Populus Tomentosa ◽  
Myb Transcription Factor ◽  
Phloem Tissue

Wood, which is mainly composed of lignified secondary cell wall, is the most abundant biomass in woody plants. Previous studies have revealed that R2R3-type MYB transcription factors are important regulators of the formation of the secondary cell wall in vascular plants. In this study, we isolated the R2R3-type MYB transcription factor gene PtoMYB055, which is mainly expressed in xylem and phloem tissue, from Populus tomentosa and demonstrate that PtoMYB055 is a key regulator of lignin biosynthesis. PtoMYB055 as a transcriptional activator is localized to the nucleus. Overexpression of PtoMYB055 upregulates expression of lignin biosynthetic genes in transgenic poplar plants, resulting in ectopic deposition of lignin in phloem tissue and an increase in thickness of the secondary cell wall. In sum, PtoMYB055 is a transcriptional activator that is involved in regulating lignin biosynthesis during the formation of the secondary cell wall in poplar.

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