Indole-3-acetic acid improves drought tolerance of white clover via activating auxin, abscisic acid and jasmonic acid related genes and inhibiting senescence genes

Maize grains (Zea mays convar. Indentata Sturt.) were treated with non-thermal plasma, where Gliding Arc plasma discharge at an atmospheric pressure was used (working gas: Air; time duration: 0 s, 180 s, 300 s, 600 s). The experiment was conducted at a temperature of 18 °C, light/dark 12/12 h, and a light intensity of 100 µmol/m2s. Seed germination, seedling growth, photosynthetic parameters, and hormone (abscisic acid, jasmonic acid, salicylic acid, indole-3-acetic acid, and cytokinin) contents were measured. The highest stimulation of seed germination (to 141%), root length (to 221%), shoot length (to 298%), and root weight (to 122%) in comparison with the control was recorded after Gliding Arc plasma treatment for 600 s. The photochemical and non-photochemical Chl fluorescence parameters were not significantly affected by Gliding Arc plasma treatment. In contrast, hormonal pools in maize were significantly affected. The short-term plasma treatment (180 s) was associated with a decrease in the stress hormones abscisic acid, salicylic acid, jasmonic acid, and jasmonate isoleucine, while indole-3-acetic acid and cytokinin precursors were elevated. Longer-term treatment (300 s, 600 s) had an opposite effect—an elevation of abscisic acid, jasmonic acid, and jasmonate isoleucine as well as active cytokinins. The content of auxin decreased. Gliding plasma treatment may significantly affect maize physiology, dependent on the treatment duration.

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Study on the extraction, purification and quantification of jasmonic acid, abscisic acid and indole-3-acetic acid in plants

Phytochemical Analysis ◽

10.1002/pca.1085 ◽

2008 ◽

Vol 19 (6) ◽

pp. 560-567 ◽

Cited By ~ 32

Author(s):

Feng Juan Zhang ◽

You Ju Jin ◽

Xing You Xu ◽

Rong Chun Lu ◽

Hua Jun Chen

Keyword(s):

Acetic Acid ◽

Abscisic Acid ◽

Jasmonic Acid ◽

Indole 3 Acetic Acid

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Indole -3-acetic acid improves drought tolerance of white clover via activating auxin, abscisic acid and jasmonic acid related genes and inhibiting senescence genes

10.21203/rs.2.16687/v4 ◽

2020 ◽

Author(s):

Youzhi Zhang ◽

Yaping Li ◽

Muhammad Jawad Hassan ◽

Zhou Li ◽

Yan Peng

Keyword(s):

Acetic Acid ◽

Drought Stress ◽

Drought Tolerance ◽

Leaf Senescence ◽

White Clover ◽

Stress Condition ◽

Drought Stress Condition ◽

Concentration Changes ◽

Indole 3 Acetic Acid ◽

Exogenous Iaa

Abstract Background: Auxin may have a positive effect on plants under drought stress. White clover is widely cultivated and often prone to water shortages. In the present study, we investigated the effects of exogenous indole -3-acetic acid (IAA) on growth and physiological changes of white clover under drought stress condition. The contents of endogenous IAA and other hormones including ABA, CTK, JA, GA, IAA, and SA were assayed. Moreover, expressions of auxin-responsive genes, drought-responsive genes and leaf senescence-associated genes were detected in response to exogenous IAA. Results: Compared to control, drought stress alone significantly diminished stem dry weigh, relative water content (RWC) and total chlorophyll content (Chl). Exogenous IAA treatment significantly increased RWC and Chl, whereas L-AOPP treatment drastically decreased stem dry weight, RWC and Chl under drought stress condition. Additionally, exogenous IAA treatment significantly increased ABA content and JA content, up-regulated expression of auxin responsive genes ( GH3.1 , GH3.9 , IAA8 ), drought stress responsive genes ( bZIP11 , DREB2 , MYB14 , MYB48 , WRKY2 , WRKY56 , WRKY108715 and RD22 ), and down-regulated expressions of auxin-responding genes ( GH3.3 , GH3.6 , IAA27 ) and leaf senescence genes ( SAG101 and SAG102 ) in the presence of PEG. Contrarily, L-AOPP treatment significantly reduced contents of ABA, GA3 and JA, down-regulated expressions of GH3.1 , GH3.9 , IAA8 , bZIP11 , DREB2 , MYB14 , MYB48 , WRKY2 , WRKY56 , WRKY108715 , ERD and RD22 , and up-regulated SAG101 and SAG102 . Conclusions: Exogenous IAA improved drought tolerance of white clover possibly due to endogenous plant hormone concentration changes and modulation of genes involving in drought stress response and leaf senescence. These results provided useful information to understand mechanisms of IAA improved drought tolerance in white clover.

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