scholarly journals Construction of a high-density genetic map and detection of a major QTL of resistance to powdery mildew (Erysiphe necator Sch.) in Caucasian grapes (Vitis vinifera L.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Tyrone Possamai ◽  
Sabine Wiedemann-Merdinoglu ◽  
Didier Merdinoglu ◽  
Daniele Migliaro ◽  
Gloria De Mori ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Vitis Vinifera ◽  
Central Asia ◽  
Genetic Relationships ◽  
High Density ◽  
Vitis Vinifera L ◽  
Resistance Locus ◽  
Phenotypic Variance ◽  
Mycelium Growth ◽  
Erysiphe Necator

Abstract Background Vitis vinifera L. is the most cultivated grapevine species worldwide. Erysiphe necator Sch., the causal agent of grape powdery mildew, is one of the main pathogens affecting viticulture. V. vinifera has little or no genetic resistances against E. necator and the grape industry is highly dependent on agrochemicals. Some Caucasian V. vinifera accessions have been reported to be resistant to E. necator and to have no genetic relationships to known sources of resistance to powdery mildew. The main purpose of this work was the study and mapping of the resistance to E. necator in the Caucasian grapes ‘Shavtsitska’ and ‘Tskhvedianis tetra’. Results The Caucasian varieties ‘Shavtsitska’ and ‘Tskhvedianis tetra’ showed a strong partial resistance to E. necator which segregated in two cross populations: the resistant genotypes delayed and limited the pathogen mycelium growth, sporulation intensity and number of conidia generated. A total of 184 seedlings of ‘Shavtsitska’ x ‘Glera’ population were genotyped through the Genotyping by Sequencing (GBS) technology and two high-density linkage maps were developed for the cross parents. The QTL analysis revealed a major resistance locus, explaining up to 80.15% of the phenotypic variance, on ‘Shavtsitska’ linkage group 13, which was associated with a reduced pathogen infection as well as an enhanced plant necrotic response. The genotyping of 105 Caucasian accessions with SSR markers flanking the QTL revealed that the resistant haplotype of ‘Shavtsitska’ was shared by ‘Tskhvedianis tetra’ and a total of 25 Caucasian grape varieties, suggesting a widespread presence of this resistance in the surveyed germplasm. The uncovered QTL was mapped in the region where the Ren1 locus of resistance to E. necator, identified in the V. vinifera ‘Kishmish vatkana’ and related grapes of Central Asia, is located. The genetic analysis conducted revealed that the Caucasian grapes in this study exhibit a resistant haplotype different from that of Central Asian grape accessions. Conclusions The QTL isolated in ‘Shavtsitska’ and present in the Caucasian V. vinifera varieties could be a new candidate gene of resistance to E. necator to use in breeding programmes. It co-localizes with the Ren1 locus but shows a different haplotype from that of grapevines of Central Asia. We therefore consider that the Caucasian resistance locus, named Ren1.2, contains a member of a cluster of R-genes, of which the region is rich, and to be linked with, or possibly allelic, to Ren1.

scholarly journals Construction of A High-Density Genetic Map And Detection of A Major QTL of Resistance To Powdery Mildew (Erysiphe Necator Sch.) In Caucasian Grapes (Vitis Vinifera L.)

2021 ◽  
Author(s):  
Tyrone Possamai ◽  
Sabine Wiedemann-Merdinoglu ◽  
Didier Merdinoglu ◽  
Daniele Migliaro ◽  
Gloria De Mori ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Vitis Vinifera ◽  
Central Asia ◽  
High Density ◽  
Vitis Vinifera L ◽  
Resistance Locus ◽  
Phenotypic Variance ◽  
Sources Of Resistance ◽  
Mycelium Growth ◽  
Erysiphe Necator

Abstract BackgroundVitis vinifera L. is the most cultivated grapevine species worldwide. Erysiphe necator Sch., the causal agent of grape powdery mildew, is one of the main pathogens affecting viticulture. V. vinifera has little or no genetic resistance against E. necator and the grape industry is highly dependent on agrochemicals. Some Caucasian V. vinifera accessions have been reported to be resistant to E. necator and to have no genetic relationship to known sources of resistance to powdery mildew. The main purpose of this work was the study and mapping of the resistance to E. necator in the Caucasian grapes ‘Shavtsitska’ and ‘Tskhvedianis tetra’.ResultsThe Caucasian varieties ‘Shavtsitska’ and ‘Tskhvedianis tetra’ showed a strong partial resistance to E. necator which segregated in two cross populations: the resistant genotypes delayed and limited the pathogen mycelium growth, sporulation intensity and number of conidia generated. A total of 184 seedlings of ‘Shavtsitska’ x ‘Glera’ population were genotyped through the Genotyping by Sequencing (GBS) technology and two high-density linkage maps were developed for the cross parents. The QTL analysis revealed a major resistance locus, explaining up to 80.15% of the phenotypic variance, on ‘Shavtsitska’ linkage group 13, which was associated with a reduced pathogen infection as well as an enhanced plant necrotic response. The genotyping of 105 Caucasian accessions with SSR markers flanking the QTL revealed that the resistant haplotype of ‘Shavtsitska’ was shared by ‘Tskhvedianis tetra’ and a total of 24 Caucasian grape varieties, suggesting a widespread presence of this resistance in the surveyed germplasm. The uncovered QTL was mapped in the region where the Ren1 gene of resistance to E. necator, identified in the V. vinifera ‘Kishmish vatkana’ and related grapes of Central Asia, is located. The genetic analysis conducted revealed that the Caucasian grapes in this study exhibit a resistant haplotype different from that of Central Asian grape accessions.ConclusionsThe QTL isolated in ‘Shavtsitska’ and present in the Caucasian V. vinifera varieties could be a new candidate gene of resistance to E. necator to use in breeding programmes. It co-localizes with the Ren1 locus but shows a different haplotype from that of grapevines of Central Asia. We therefore consider this gene, named Ren1-2, a member of a cluster of R genes, of which the region is rich, and linked with, or possibly allelic, to the Ren1.

scholarly journals Genetic structure and differentiation in cultivated grape, Vitis vinifera L.

2003 ◽  
Vol 81 (3) ◽  
pp. 179-192 ◽  
Author(s):  
MALLIKARJUNA K. ARADHYA ◽  
GERALD S. DANGL ◽  
BERNARD H. PRINS ◽  
JEAN-MICHEL BOURSIQUOT ◽  
M. ANDREW WALKER ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Vitis Vinifera ◽  
Gene Diversity ◽  
Genetic Relationships ◽  
Vitis Vinifera L ◽  
Significant Heterogeneity ◽  
Multivariate Relationships ◽  
History Of ◽  
Single Complex ◽  
Grape Cultivars

222 cultivated (Vitis vinifera) and 22 wild (V. vinifera ssp. sylvestris) grape accessions were analysed for genetic diversity and differentiation at eight microsatellite loci. A total of 94 alleles were detected, with extensive polymorphism among the accessions. Multivariate relationships among accessions revealed 16 genetic groups structured into three clusters, supporting the classical eco-geographic grouping of grape cultivars: occidentalis, pontica and orientalis. French cultivars appeared to be distinct and showed close affinity to the wild progenitor, ssp. sylvestris from south-western France (Pyrenees) and Tunisia, probably reflecting the origin and domestication history of many of the old wine cultivars from France. There was appreciable level of differentiation between table and wine grape cultivars, and the Muscat types were somewhat distinct within the wine grapes. Contingency χ2 analysis indicated significant heterogeneity in allele frequencies among groups at all loci. The observed heterozygosities for different groups ranged from 0·625 to 0·9 with an overall average of 0·771. Genetic relationships among groups suggested hierarchical differentiation within cultivated grape. The gene diversity analysis indicated narrow divergence among groups and that most variation was found within groups (∼85%). Partitioning of diversity suggested that the remaining variation is somewhat structured hierarchically at different levels of differentiation. The overall organization of genetic diversity suggests that the germplasm of cultivated grape represents a single complex gene pool and that its structure is determined by strong artificial selection and a vegetative mode of reproduction.

The Effect of Downy and Powdery Mildew on Grapevine (Vitis vinifera L.) Leaf Gas Exchange

2005 ◽  
Vol 153 (6) ◽  
pp. 350-357 ◽  
Author(s):  
M. Moriondo ◽  
S. Orlandini ◽  
A. Giuntoli ◽  
M. Bindi
Keyword(s):  
Gas Exchange ◽  
Powdery Mildew ◽  
Vitis Vinifera ◽  
Leaf Gas Exchange ◽  
Vitis Vinifera L

scholarly journals Powdery mildew infection dependent on weather factors in vineyards near Keszthely in 2008

2010 ◽  
Vol 16 (2) ◽  
Author(s):  
P. Taksony ◽  
E. Tarczal ◽  
K. Maráczi ◽  
I. J. Holb ◽  
L. Kocsis
Keyword(s):  
High Temperature ◽  
Powdery Mildew ◽  
Vitis Vinifera ◽  
Meteorological Data ◽  
Weather Conditions ◽  
Vitis Vinifera L ◽  
Weather Factors ◽  
Vegetative Period ◽  
Favorable Conditions ◽  
Powdery Mildew Infection

Weather conditions are extremely influential on grapevine productivity and quality. High temperature and humidity makes favorable conditions for powdery mildew infection respectively. The meteorological data around Keszthely, Hungary show the vegetative period is dryer and warmer than it was closely hundred years ago. We examined the development of powdery mildew infection  on  two variet ies Vitis vinifera L. cv Italian Riesling and cv Merlot in relation with meteorological data. No primer infections were appeared in the vineyard. The year of 2008 was quite ideal for the accumulation of Erysiphe necator in the experimental vineyard. Although the dry summer can lower the infection, but if the high temperature is coupling with rainfall, the possibilities of powdery mildew infection is going to grow higher during the upcoming years.

Flashes of UV-C light stimulate defences of Vitis vinifera L. cv. Chardonnay against Erysiphe necator in greenhouse and vineyard conditions

Plant Disease ◽  
2021 ◽  
Author(s):  
Loïc Ledermann ◽  
Stéphane Daouda ◽  
Caroline Gouttesoulard ◽  
Jawad Aarrouf ◽  
Laurent Urban
Keyword(s):  
Vitis Vinifera ◽  
Symptom Severity ◽  
Dark Respiration ◽  
Net Photosynthesis ◽  
Vitis Vinifera L ◽  
Plant Defences ◽  
Erysiphe Necator ◽  
Detached Leaves ◽  
Uv C ◽  
Better Than

Using detached leaves, UV-C light in the form of 1-sec flashes has recently been shown to stimulate defences of several plants against different pathogenes better than 1 min exposures under greenhouse conditions. In the present work, the pathological tests were conducted using undetached leaves under greenhouse and vineyard conditions. In a first trial, two flashes of UV-C light were applied to plants of Vitis vinifera L. cv. Chardonnay grown under greenhouse conditions, at an interval of 10 days. Plants were inoculated with Erysiphe necator two days after the last light treatment. After 18 days of inoculation, the symptom severity on leaves was reduced by 60 % when compared with the untreated control. In a second trial, flashes of UV-C light were applied to grapevine Chardonnay plants under field conditions in the South-East of France, every 10 days from the 18th of April until the 10th of July 2019. The symptom severity resulting from natural contaminations by Erysiphe necator was reduced by 42 % in leaves on the 4th of July 2019 and by 65 % in clusters on the 25th of July 2019. In a third trial, we observed that UV-C light did not have any effect on net photosynthesis, maximal net photosynthesis, dark respiration, maximal quantum efficiency of photosystem II, the performance index of Strasser and, generally, any parameter derived from induction curves of maximal chlorophyll fluorescence. It was concluded that flashes of UV-C light have true potential for stimulating plant defences against Erysiphe necator under vineyard conditions and, therefore, help in reducing fungicide use.

Release, germination, and pathogenicity of ascospores of Uncinula necator under controlled conditions

1998 ◽  
Vol 76 (5) ◽  
pp. 777-781 ◽  
Author(s):  
F Jailloux ◽  
T Thind ◽  
M Clerjeau
Keyword(s):  
Powdery Mildew ◽  
Vitis Vinifera ◽  
Storage Conditions ◽  
Vitis Vinifera L ◽  
Uncinula Necator ◽  
Grapevine Powdery Mildew ◽  
Germination Ability ◽  
Ascospore Release ◽  
Probable Role ◽  
Leaf Disks

A laboratory technique was standardized for studying the release, maturation, germination, and pathogenicity of ascospores of Uncinula necator (Schw.) Burr. Surface disinfestation and wetting of grape (Vitis vinifera L.) leaf disks bearing cleistothecia collected in the vineyard before incubation in a humid chamber for 48 h at 20°C were found essential for obtaining the release of ascospores (8 ascospores/cm2). Storage conditions involving periodic wetting treatments of cleistothecia at 5°C during 110 days were necessary to induce both ascospore release (80 ascospores/cm2) and germination ability (62%). The matured ascospores were pathogenic on healthy leaf disks at 20°C indicating their probable role as a primary inoculum source. This technique can be helpful in studying the inheritance of characteristics such as pathogenicity and fungicidal resistance.Key words: cleistothecia, ascospores, maturation, germination, pathogenicity, Uncinula necator, grapevine, powdery mildew.

Vitis Species from the Southwestern United States Vary in Their Susceptibility to Powdery Mildew

Plant Disease ◽  
2021 ◽  
pp. PDIS-10-20-2103
Author(s):  
Laila Fayyaz ◽  
Alan Tenscher ◽  
Andy Viet Nguyen ◽  
Huma Qazi ◽  
M. Andrew Walker
Keyword(s):  
United States ◽  
Powdery Mildew ◽  
Vitis Vinifera ◽  
Resistance Breeding ◽  
Vitis Vinifera L ◽  
Southwestern United States ◽  
Breeding Programs ◽  
Vitis Species ◽  
Northern Mexico ◽  
Eastern Species

The European grapevine (Vitis vinifera L.) has been cultivated in North America for about 500 years. One of the major limitations to its culture is the powdery mildew (PM) fungus, Erysiphe necator Schw. This study reports on the most extensive screening of Vitis species from the southwestern United States and northern Mexico for resistance to PM, testing 147 accessions of 13 Vitis species. In addition, Vitis vinifera cv. Carignane, a highly susceptible wine grape cultivar, was used as a reference to evaluate the effect of the inoculum 14 days postinoculation. Inoculation was done with a vacuum-operated settling tower using a broadly virulent isolate of E. necator, the C-strain. Resistant accessions (nine), moderately susceptible accessions (39), and highly susceptible accessions (99) were detected. The resistant accessions were then inoculated with an additional fungal isolate, e1-101, and they retained their resistance. Vitis species susceptibility was not associated with a North-South gradation, but Western species were more susceptible than Midwestern and Eastern species. All five of the V. monticola accessions were susceptible, as were the accessions of V. treleasei. The species V. acerifolia, V. candicans, V. cinerea, and V. × doaniana had significantly more resistant to moderately susceptible accessions compared with V. arizonica, V. berlandieri, V. californica, V. × champinii, V. girdiana, V. riparia, and V. rupestris, which had relatively more susceptible accessions than the other species. This research identified new sources of PM resistance in Vitis from the southwestern United States that could be incorporated into PM resistance breeding programs throughout the world.

In silico development of microsatellites associated with resistance genes to powdery mildew in Vitis vinifera L.

2020 ◽  
pp. 493-498
Author(s):  
S. Mojemmi ◽  
M. Mdarhri Alaoui ◽  
F. Rchok ◽  
Z.E.A. Triqui ◽  
O. Chlyah ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Vitis Vinifera ◽  
Resistance Genes ◽  
In Silico ◽  
Vitis Vinifera L
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