scholarly journals Long non-coding RNA BRE-AS1 inhibits the proliferation, migration, and invasion of cancer cells in triple-negative breast cancer and predicts patients’ survival by downregulating miR-21

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jianchao Gao ◽  
Sisi Wang ◽  
Zhisheng Zhang ◽  
Jun Li
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Direct Interaction ◽  
Migration And Invasion ◽  
Control Group ◽  
Expression Levels ◽  
Cell Behaviors ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Abstract Background BRE-AS1 is a recently identified tumor suppressor in non-small cell lung cancer. It role in other human diseases remains elusive. Methods Differential expression of BRE-AS1 in with triple-negative breast cancer (TNBC) patients (n = 74, patient group) and healthy volunteers (n = 58, control group) was studied with RT-qPCR. The direct interaction between BRE-AS1 and premature microRNA-21 (miR-21) was assessed by RNA pull-down assay. The interactions among BRE-AS1, miR-21 and PTEN were evaluated by overexpression assays. CCK-8 assay and Transwell assay were used to evaluate cell behaviors. Results BRE-AS1 was downregulated in TNBC, while miR-21 was highly expressed in TNBC. Low expression levels of lncRNA BRE-AS1 and high expression levels of miR-21 were significantly correlated with unfavorable survival outcomes. BRE-AS1 and miRNA-21 were inversely correlated across TNBC samples, not control samples. BRE-AS1 decreased miR-21 expression and increased PTEN expression while miR-21showed no role in BRE-AS1 expression. RNA pull-down assay illustrated that BRE-AS1 may sponge premature miR-21 to suppress it maturation. Overexpression of BRE-AS1 decreased cell behaviors, while overexpression of miR-21 promoted cell behaviors. MiR-21 suppressed the role of BRE-AS1 in cancer cell behaviors. Conclusion Therefore, BRE-AS1 may inhibit TNBC by downregulating miR-21.

scholarly journals The long non-coding RNA ADAMTS9-AS2 was identified as a potential prognostic biomarker in triple-negative breast cancer

2020 ◽  
Vol 6 (3) ◽  
pp. 16215-16226
Author(s):  
Daniel Rodrigues Bastos ◽  
Jean Michel Rocha Sampaio Leite ◽  
Mércia Patrícia Ferreira Conceição ◽  
Cesar Augusto Sam Tiago Vilanova-Costa ◽  
Antonio Márcio Teodoro Cordeiro Silva ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Prognostic Biomarker ◽  
Non Coding Rna ◽  
Long Non Coding Rna

scholarly journals MicroRNA-224 Promotes Tumorigenesis through Downregulation of Caspase-9 in Triple-Negative Breast Cancer

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Li Zhang ◽  
Xin Zhang ◽  
Xin Wang ◽  
Miao He ◽  
Shixing Qiao
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Direct Interaction ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Breast Cancer Cell Lines ◽  
Protein Levels ◽  
Reporter Assays ◽  
Novel Therapeutic Strategies

Triple-negative breast cancer (TNBC) harbors genetic heterogeneity and generally has more aggressive clinical outcomes. As such, there is urgency in identifying new prognostic targets and developing novel therapeutic strategies. In this study, miR-224 was overexpressed in breast cancer cell lines and TNBC primary cancer samples. Knockdown of miR-224 in MDA-MB-231 cancer cells reduced cell proliferation, migration, and invasion. Through integrating in silico prediction algorithms with KEGG pathway and Gene Ontology analyses, CASP9 was identified to be a potential target of miR-224. miR-224 knockdown significantly increased CASP9 transcript and protein levels. Furthermore, luciferase reporter assays confirmed a direct interaction of miR-224 with CASP9. Our findings have demonstrated that the miR-224/CASP9 axis plays an important role in TNBC progression, providing evidence in support of a promising therapeutic strategy for this disease.

Long non-coding RNA TUG1 sponges miR-197 to enhance cisplatin sensitivity in triple negative breast cancer

2018 ◽  
Vol 107 ◽  
pp. 338-346 ◽  
Author(s):  
Tielei Tang ◽  
Yonggang Cheng ◽  
Qing She ◽  
Yaru Jiang ◽  
Yuanyuan Chen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Non Coding Rna ◽  
Cisplatin Sensitivity ◽  
Long Non Coding Rna

scholarly journals MiR-212-5p Suppresses the Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer by Targeting Prrx2

2017 ◽  
Vol 44 (5) ◽  
pp. 1785-1795 ◽  
Author(s):  
Zhi-Dong Lv ◽  
Dong-Xia Yang ◽  
Xiang-Ping Liu ◽  
Li-Ying Jin ◽  
Xin-Gang Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Progression ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Ectopic Expression ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Lymph Node Status ◽  
Mesenchymal Transition ◽  
Expression Levels

Background/Aims: Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype. Our study investigated the functional role of miR-212-5p in TNBC. Methods: Realtime PCR was used to quantify miR-212-5p expression levels in 30 paired TNBC samples and adjacent normal tissues. Wound healing and Transwell assays were used to evaluate the effects of miR-212-5p expression on the invasiveness of TNBC cells. Luciferase reporter and Western blot assays were used to verify whether the mRNA encoding Prrx2 is a major target of miR-212-5p. Results: MiR-212-5p was downregulated in TNBC, and its expression levels were related to tumor size, lymph node status and vascular invasion in breast cancer. We also observed that the miR-212-5p expression level was significantly correlated with a better prognosis in TNBC. Ectopic expression of miR-212-5p induced upregulation of E-cadherin expression and downregulation of vimentin expression. The expression of miR212-5p also suppressed the migration and invasion capacity of mesenchymal-like cancer cells accompanied by a morphological shift towards the epithelial phenotype. Moreover, our study observed that miR-212-5p overexpression significantly suppressed Prrx2 by targeting its 3’-untranslated region (3’-UTR) region, and Prrx2 overexpression partially abrogated miR-212-5p-mediated suppression. Conclusions: Our study demonstrated that miR-212-5p inhibits TNBC from acquiring the EMT phenotype by downregulating Prrx2, thereby inhibiting cell migration and invasion during cancer progression.

Long non-coding RNA ANRIL promotes carcinogenesis via sponging miR-199a in triple-negative breast cancer

2017 ◽  
Vol 96 ◽  
pp. 14-21 ◽  
Author(s):  
Shuang-Ta Xu ◽  
Jian-Hua Xu ◽  
Zheng-Rong Zheng ◽  
Qing-Quan Zhao ◽  
Xiao-Shan Zeng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Modulated electro‐hyperthermia suppresses H19, a tumor promoting long non coding RNA, in a triple‐negative breast cancer model

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Lea Danics ◽  
Csaba András Schvarcz ◽  
Zita Zolcsák ◽  
Zoltán Benyó ◽  
Tamás Kaucsár ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Cancer Model ◽  
Non Coding Rna ◽  
Breast Cancer Model ◽  
Long Non Coding Rna
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