scholarly journals The accuracy of gypsum casts obtained from the disinfected extended-pour alginate impressions through prolonged storage times

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Rania A. Sharif ◽  
Khalid M. Abdelaziz ◽  
Najla M. Alshahrani ◽  
Fatimah S. Almutairi ◽  
Mohrah A. Alaseri ◽  
...  

Abstract Background Manufacturers of the extended-pour alginates claimed their dimensional stability through prolonged storage. No data confirmed the ability of these materials to maintain their dimensions and the reproduced oral details following their chemical disinfection. Therefore, this study evaluated the dimensional stability and surface detail reproduction of gypsum casts obtained from disinfected extended-pour alginate impressions through different storage time intervals. Methods Two hundred and forty three hydrocolloid impressions were made from one conventional (Tropicalgin) and two extended-pour (Hydrogum 5 and Chromaprint premium) alginates. These impressions were subjected to none, spray and immersion disinfection before their storage in 100% humidity for 0, 72 and 120 h. The dimensional stability and the surface detail reproduction were indirectly evaluated under low angle illumination on the resulted gypsum casts. At α = 0.05, the parametric dimensional stability data were analyzed using One-Way ANOVA and Tukey’s comparisons, while the nonparametric detail reproduction data were analyzed using KrusKal Wallis and Mann–Whitney's tests. Results All gypsum casts exhibited a degree of expansion; however, the recorded expansion values did not differ between test categories (P > 0.05). Generally, casts obtained from spray-disinfected impressions showed lower detail accuracy (P < 0.05). Immersion-disinfected extended-pour alginates produced casts with better detail accuracy following 120 h of storage (P < 0.05). Conclusion All alginates materials offer comparable cast dimensions under different testing circumstances. Extended-pour alginates offer casts with superior surface details following their immersion disinfection and 120 h of storage. Spray-disinfection using 5.25% sodium hypochlorite affects the surface details of casts obtained from conventional and extended-pour alginates adversely.

2020 ◽  
Vol 11 (1) ◽  
pp. 10
Author(s):  
SuryakantC Deogade ◽  
Monika Khatri ◽  
SnehaS Mantri ◽  
Abhilasha Bhasin ◽  
Shivkumar Mantri ◽  
...  

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Qudrati Rohmah ◽  
Hari Santoso ◽  
Hasan Zayadi

Cryopreservation semen is one of the techniques used to defend the life-cell storage technique and the structural intact network in the long term at very low temperatures, but the use of improper diluent solutions in Cooling may cause abnormalities in the cells. The purpose of this research is to analyze the influence of the immaturity of spermatozoa clotting combination of coconut water, egg yolks, and glycerol against a goldfish's spermatozoa abnormality (Cyprinus carpio L.) with two different storage times. The research method of using spermatozoa thinners with the treatment of 3 variant combinations namely: A1 (70% coconut water: 20% egg yolks: 10% glycerol); A2 (60% coconut water: 30% egg yolks: 10% glycerol); A3 (50% coconut water: 40% egg yolks: 10% glycerol) each stored for 24 hours and 48h, with 4 x repeats. The research material is a goldfish spermatozoa (Cyprinus carpio L.) taken from a mature gonads. A test of spermatozoa abnormalities is performed using five points of view for each sample, calculated by the abnormalities of spermatozoa with the formula of abnormalities, and analyses using the One Way ANOVA test. The results showed that the combination of the 24 hour shelf life of A1 was not significantly different to the fresh sperm abnormalities. In the introduction of the A2 combination, and A3 indicates significantly different with the sig (0.022); Long storage time affects the abnormality of spermatozoa with sig (0.001) This suggests that the longer the Spermatozoa storage in a combination of thinners occurs increased abnormalities of the goldfish spermatozoa (Cyprinus carpio L).Keywords: abnormalities, spermatozoa, glycerol, Cyprinus carpio LABSTRAKKriopreservasi semen merupakan teknik untuk mempertahakan penyimpanan sel hidup dan jaringan yang utuh secara struktural dalam jangka panjang di suhu sangat rendah, larutan pengencer yang tidak tepat dalam pendinginan dapat menyebabkan abnormalitas pada sel. Tujuan penelitian ini untuk menganalisa pengaruh pemberian pengencer pembekuan spermatozoa kombinasi air kelapa, kuning telur, dan gliserol terhadap abnormalitas spermatozoa ikan mas (Cyprinus carpio L.) pada dua waktu penyimpanan yang berbeda. Metode penelitian menggunakan pengencer spermatozoa dengan perlakuan 3 varian kombinasi yakni :A1 (70% air  kelapa : 20% kuning telur : 10% gliserol); A2(60% airkelapa : 30% kuning telur : 10% gliserol); A3 (50% air kelapa : 40% kuning telur : 10% gliserol) yang masing-masing disimpan selama 24 jam dan 48jam, dengan 4 x ulangan. Bahan penelitian adalah spermatozoa ikan mas(Cyprinus carpio L.) diambil dari gonad yang matang. Uji abnormalitas spermatozoa dilakukan menggunakan lima lapang pandang untuk masing-masing sampel, dihitung abnormalitas spermatozoa dengan rumus abnormalitas, dan dianalisis menggunakan uji One Way Anova. Hasil penelitian menunjukkan  bahwa pemberian kombinasi A1 masa simpan 24jam tidak berbeda secara signifikan terhadap abnormalitas sperma segar. Pada pemberian kombinasi A2, dan A3 menunjukkan berbeda signifikan dengan sig (0,022); lama waktu penyimpanan berpengaruh terhadap abnormalitas spermatozoa dengan sig (0,001) hal ini menunjukkan bahwa pemberian kombinasi pengencer yang tepat dapat digunakan  dalammempertahakan nilai normalitas pada pengawetan spermatozoa ikan mas (Cyprinus carpio L).Kata kunci :Abnormalitas, spermatozoa, gliserol, Cyprinus carpio L


RSBO ◽  
2019 ◽  
Vol 15 (2) ◽  
pp. 77
Author(s):  
Lidia Olga Bach Pinheiro ◽  
Alfonso Sanchez Ayala ◽  
Marcos Cezar Pomini ◽  
Vanessa Taborda da Cruz ◽  
Ingrid Viel del Farias ◽  
...  

Irreversible hydrocolloids have been used in dental practice for decades and their pouring time has been suggested to be as short as possible; however the latest generation of alginates exhibit better properties, which could influence the fidelity of the casts. Objective: To evaluate the linear dimensional stability of stone casts obtained using two irreversible hydrocolloid brands (Cavex ColorChange and Jeltrate) after disinfection. Material and methods: Samples were divided into 16 groups (n = 10) according to brand, storage time and disinfection. Impressions were taken of a metallic master model made of stainless steel with two abutments. Cast models were obtained using type IV gypsum, after disinfection with sodium hypochlorite at 1% or without any disinfection followed by storage for four different times (immediate pouring, and after 24, 72 or 120 hours storage after obtaining the impressions) and the models were measured with a digital caliper. Data were submitted to ANOVA 3-way followed by Tukey’s test (α < 0.05). Results: A statistically significant difference was detected with Jeltrate Plus after 72 and 120 hours in storage (with and without disinfection), as well as Cavex without disinfection. Differences were found for Cavex with disinfection/24 hours storage, when compared to immediate pouring (P < 0.05). The alginates presented similar behavior regarding disinfection for the same time (P > 0.05). Conclusion: Storage longer than 24 hours affects the fidelity of casts. Disinfection does not promote significant alteration at any of the experimental times with either material.


2020 ◽  
Author(s):  
Rania A. Sharif ◽  
Khalid M. Abdelaziz ◽  
Najla Manis Zafer Alshahrani ◽  
Fatimah Salem Almutairi ◽  
Mohrah Awadh Saeed Alaseri ◽  
...  

Abstract Background: Many new extended-pour irreversible hydrocolloid impression materials have been marketed with claims that they demonstrate enhanced surface details accuracy and dimensional stability for up to 120 hours of storage. However, no data in the literature about the effect of storage time on surface details and dimensional stability of extended-pour impression materials upon disinfection. In this study we aimed to evaluate the effect of two chemical disinfection methods and three storage times on dimensional stability and surface details reproduction of 2 extended-pour alginates in comparison with a conventional one.Methods: A total of 243 alginate impressions were recorded from a custom metal die using Tropicalgin (conventional), Hydrogum5 and Chroma print (extended-pour) alginates. Impressions were categorized into 9 groups according to disinfection method (non-disinfected, sprayed or immersed) and storage time (0, 72, 120 hours). After storage in sealed plastic bag with a damp cotton roll, all impressions were poured with type IV dental stone. The stone models were then examined for surface details and dimensions using a magnifier (×10) and digital caliper to assess whether a 25 µm-line on the metal die was completely reproduced over the full 25 mm length and its dimensions compared to the original die. Data were analyzed using one-way (ANOVA) and Tuckey’s post-hoc test, and KrusKal Wallis test and Mann-Whitney's test.Results: With immediate pouring all alginates showed accurate surface details regardless of the disinfection condition, except for immersed Tropicalgin (P<0.05). The extended storage affected surface details of all materials. After 72h, only non-disinfected extended-pour alginates showed dimensional stability. However, after 120h of storage all materials regained dimensional stability except sprayed Tropicalgin.ConclusionsExtended-pour alginates hardly proved to be more advantageous than traditional alginate in terms of surface details accuracy. Disinfection methods had some adverse effects on surface details and dimensional accuracy of both extended-pour and conventional alginate. Extended-pour alginate proved dimensional stability after 120h. It is recommended to immediately pour all alginates whether conventional or extended-pour. Further studies are needed to evaluate impression materials directly to control the confounding variable like gypsum expansion and storage environment in terms of humidity and temperature.


RSBO ◽  
2018 ◽  
Vol 15 (2) ◽  
pp. 77-83
Author(s):  
Lidia Olga Bach Pinheiro ◽  
Alfonso Sanchez Ayala ◽  
Marcos Cezar Pomini ◽  
Vanessa Taborda da Cruz ◽  
Ingrid Viel del Farias ◽  
...  

Irreversible hydrocolloids have been used in dental practice for decades and their pouring time has been suggested to be as short as possible; however the latest generation of alginates exhibit better properties, which could influence the fidelity of the casts. Objective: To evaluate the linear dimensional stability of stone casts obtained using two irreversible hydrocolloid brands (Cavex ColorChange and Jeltrate) after disinfection. Material and methods: Samples were divided into 16 groups (n = 10) according to brand, storage time and disinfection. Impressions were taken of a metallic master model made of stainless steel with two abutments. Cast models were obtained using type IV gypsum, after disinfection with sodium hypochlorite at 1% or without any disinfection followed by storage for four different times (immediate pouring, and after 24, 72 or 120 hours storage after obtaining the impressions) and the models were measured with a digital caliper. Data were submitted to ANOVA 3-way followed by Tukey’s test (α < 0.05). Results: A statistically significant difference was detected with Jeltrate Plus after 72 and 120 hours in storage (with and without disinfection), as well as Cavex without disinfection. Differences were found for Cavex with disinfection/24 hours storage, when compared to immediate pouring (P < 0.05). The alginates presented similar behavior regarding disinfection for the same time (P > 0.05). Conclusion: Storage longer than 24 hours affects the fidelity of casts. Disinfection does not promote significant alteration at any of the experimental times with either material.


Author(s):  
Robert Corbett ◽  
Delbert E. Philpott ◽  
Sam Black

Observation of subtle or early signs of change in spaceflight induced alterations on living systems require precise methods of sampling. In-flight analysis would be preferable but constraints of time, equipment, personnel and cost dictate the necessity for prolonged storage before retrieval. Because of this, various tissues have been stored in fixatives and combinations of fixatives and observed at various time intervals. High pressure and the effect of buffer alone have also been tried.Of the various tissues embedded, muscle, cartilage and liver, liver has been the most extensively studied because it contains large numbers of organelles common to all tissues (Fig. 1).


Proceedings ◽  
2021 ◽  
Vol 52 (1) ◽  
pp. 2
Author(s):  
Noé Anes García ◽  
Antonio Luis Marqués Sierra

In recent years, developments made to reduce the consequences generated using petroleum products have been strengthening; therefore, biofuels have become a requirement in different countries worldwide with the objective of reducing not only the high levels of current pollution, but also mitigating the effects generated by global warming. Despite the advances that have been made in the field of research on Jatropha, it is still necessary to carry out more detailed studies aimed at achieving a better use of it, identifying the influence of its physical–chemical properties in terms of quality levels, as well as determining its behavior when mixed with palm oil to achieve a biodiesel with better yields, whose impact will be reflected mainly in the environmental field, helping to mitigate the production of greenhouse gases that are produced by petroleum products. Although currently the biofuels sector has made important advances in research, it is necessary to deepen the physical–chemical analyses both in the production and storage processes of biodiesel, so that in the future it can be fully fulfilled with the energy requirements that are currently only achieved with fossil fuels, so it is necessary to direct this research toward the development of new products with improved characteristics, especially when exposed to prolonged storage times and low temperatures.


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