Effect of a UV-C Automatic Last-Generation Mobile Robotic System on Multi-Drug Resistant Pathogens

Background: Healthcare-associated infections caused by multi-drug resistant (MDR) pathogens are associated with increased mortality and morbidity among hospitalized patients. Inanimate surfaces, and in particular high-touch surfaces, have often been described as the source for outbreaks of nosocomial infections. The present work aimed to evaluate the efficacy of a last-generation mobile (robotic) irradiation UV-C light device R2S on MDR microorganisms in inanimate surfaces and its translation to hospital disinfection. Methods: The efficacy of R2S system was evaluated in environmental high-touch surfaces of two separate outpatient rooms of Perugia Hospital in Italy. The static UV-C irradiation effect was investigated on both the bacterial growth of S. aureus, MRSA, P. aeruginosa, and K. pneumoniae KPC and photoreactivation. The antimicrobial activity was also tested on different surfaces, including glass, steel, and plastic. Results: In the environmental tests, the R2S system decreased the number of bacteria, molds, and yeasts of each high-touch spot surface (HTSs) compared with manual sanitization. UV-C light irradiation significantly inhibits in vitro bacterial growth, also preventing photoreactivation. UV-C light bactericidal activity on MDR microorganisms is affected by the type of materials of inanimate surfaces. Conclusions: The last-generation mobile R2S system is a more reliable sanitizing procedure compared with its manual counterpart.

Coronavirus Disinfection Physical Methods

Since 2019, the spread of the Coronavirus pandemic becomes the global health crisis. To fight the pandemic, several measures were adopted such as: Hygiene measure, massive test, social distancing, quarantine and distancing. Disinfection is an important operation in the fight against the spread of Corona virus pandemic. The disinfection methods are of chemical and physical type. In this work, we focused our interest to the physical methods. These methods are classified in three principal categories: irradiation techniques, heat treatment and mechanical techniques. All the different aspect of techniques are exposed in this chapter. The efficiency of the used techniques is also discussed.

Effectiveness of UVC, Ozone and Negative Ions against Bacteria and Viruses

Since the beginning of time, viruses and bacteria have been a part of human life. Several types of sterilization systems were used to disinfect these viruses and bacteria all over the world. These methods were varied according to ethnics. With the advancement of technology, people used to find new disinfection methods. Ultraviolet, Ozone, Negative Ions were some of those methods that were used to disinfect bacteria and viruses. Most of the disinfector units, use one of the above-mentioned methods for disinfection. The aim is to carry out various experiments using ultraviolet light, ozone, and ionization under different environmental conditions to measure the efficiency of these disinfection methods

Effectiveness of UVC, Ozone and Negative Ions against Bacteria and Viruses

Since the beginning of time, viruses and bacteria have been a part of human life. Several types of sterilization systems were used to disinfect these viruses and bacteria all over the world. These methods were varied according to ethnics. With the advancement of technology, people used to find new disinfection methods. Ultraviolet, Ozone, Negative Ions were some of those methods that were used to disinfect bacteria and viruses. Most of the disinfector units, use one of the above-mentioned methods for disinfection. The aim is to carry out various experiments using ultraviolet light, ozone, and ionization under different environmental conditions to measure the efficiency of these disinfection methods

Disinfection of the root canal system: what should the protocol be?

The presence of micro-organisms within the root canal system is the critical aetiological factor in peri-radicular periodontitis. During root canal treatment (RCT) it is imperative that this infection and other organic debris are removed from the root canal system. This is challenging because complex tooth anatomy, the presence of a biofilm and the smear layer complicate the process. There are a number of irrigant chemicals and adjunctive systems available in contemporary endodontic practice that are used to disinfect the root canal system during root canal preparation. This article reviews the available evidence concerning these disinfection methods and concludes by presenting a clinical protocol supported by the literature. CPD/Clinical Relevance: A clinical protocol, supported by the literature, is presented for effective decontamination of the root canal system during root canal therapy.

Efficiency of coronavirus inactivation on environmental surfaces: A comparison study of two available disinfectants

Background: There are many coronaviruses of significant medical and veterinary concern, all of which are the result of spillover from another species. Disinfection of healthcare and veterinary environments is an important factor in limiting the transmission of coronaviruses. Disinfection agents for coronaviruses use bleach, quaternary compounds, hydrogen peroxide, and sodium hydroxide. Product labels list contact times that range from 10-30 minutes for total inactivation. Decon7 is a combination disinfectant that is currently used in the food and agriculture, medical facilities, and other industries. While Decon7 has been shown to inactivate a variety of pathogens and disrupt biofilms, its effectiveness and rate of coronavirus inactivation has not been evaluated.Objective: This project sought to evaluate Decon7’s effectiveness and rate of coronavirus inactivation.Methods: This study evaluated the disinfection efficacy of Decon7 (diluted at 1:4) and bleach (diluted at 1:10) after 3 coronaviruses (SARS-CoV-2, HCoV OC43, and HCoV NL63) were inoculated onto up to sixteen environmental surface materials.Results: A 1:4 dilution of Decon7 inactivated all coronaviruses on all surfaces with 1 minute contact time. A 1:10 dilution of bleach was not effective in inactivating coronaviruses with a contact time of 1 minute on all surfaces.Conclusions: New technologies and chemistries may offer more efficient inactivation of pathogens on environmental surfaces. These disinfection methods and materials, which require less than 10 minutes contact time, may improve the efficacy of cleaning and disinfecting surfaces in the built environment.