A rapid and versatile tool for genomic engineering in Lactococcus lactis

Microbes use diverse defence strategies that allow them to withstand exposure to a variety of genome invaders such as bacteriophages and plasmids. One such defence strategy is the use of RNA guided endonuclease called CRISPR-associated (Cas) 9 protein. The Cas9 protein, derived from type II CRISPR/Cas system, has been adapted as a versatile tool for genome targeting and engineering due to its simplicity and high efficiency over the earlier tools such as ZFNs and TALENs. With recent advancements, CRISPR/Cas9 technology has emerged as a revolutionary tool for modulating the genome in living cells and inspires innovative translational applications in different fields. In this paper we review the developments and its potential uses in the CRISPR/Cas9 technology as well as recent advancements in genome engineering using CRISPR/Cas9.

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LC-MS/MS as versatile tool in therapeutic drug monitoring

10.1055/s-0038-1649544 ◽

2018 ◽

Author(s):

G Zurek

Keyword(s):

Therapeutic Drug Monitoring ◽

Drug Monitoring ◽

Therapeutic Drug ◽

Versatile Tool

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AAZTA5.SA.KuE, a versatile tool for theranostic application – labeling, in vitro, and first in vivo-investigation of [ 177 Lu]Lu- AAZTA 5 .SA.KuE

10.1055/s-0040-1708205 ◽

2020 ◽

Author(s):

L Greifenstein ◽

T Grus ◽

D Mathé ◽

R Bergmann ◽

F Rösch

Keyword(s):

Versatile Tool

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Intestinal delivery of proinsulin and IL-10 via Lactococcus lactis combined with low-dose antiCD3 induces antigen-specific FoxP3+ Tregs in autoimmune diabetic mice

Endocrine Abstracts ◽

10.1530/endoabs.71.004 ◽

2020 ◽

Author(s):

Martens P-J ◽

Sassi G ◽

Viaene M ◽

Laureys J ◽

Teyton L ◽

...

Keyword(s):

Lactococcus Lactis ◽

Low Dose ◽

Diabetic Mice ◽

Intestinal Delivery

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Characterization of mesenchymal stem cells and their application in experimental embryology

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2013-0084 ◽

2013 ◽

Vol 16 (3) ◽

pp. 593-599 ◽

Cited By ~ 3

Author(s):

J. Opiela ◽

M. Samiec

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Somatic Cell ◽

Fertilization In Vitro ◽

Cell Cloning ◽

Mammalian Embryo ◽

Vitro Fertilization ◽

Experimental Embryology ◽

Genomic Engineering

Abstract The efficiency of somatic cell cloning (somatic cell nuclear transfer; SCNT) as well as in vitro fertilization/in vitro embryo production (IVF/IVP) in mammals stay at relatively same level for over a decade. Despite plenty of different approaches none satisfactory break-through took place. In this article, we briefly summarize the implementation of mesenchymal stem cells (MSCs) for experimental embryology. The advantages of using MSCs as nuclear donors in somatic cell cloning and in vitro embryo culture are described. The description of results obtained with these cells in mammalian embryo genomic engineering is presented.

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CRUCIBLE S7

Alloy Digest ◽

10.31399/asm.ad.ts0543 ◽

1996 ◽

Vol 45 (7) ◽

Keyword(s):

Heat Treatment ◽

Fracture Toughness ◽

Physical Properties ◽

Tool Steel ◽

Cold Work ◽

Heat Treating ◽

High Shock ◽

Unusual Combination ◽

Versatile Tool ◽

Shock Resistance

Abstract Crucible S7 is a chromium/molybdenum tool steel developed to produce the unusual combination of high shock resistance and toughness together with ease of machining and heat treatment. It is a versatile tool steel applicable for both hot and cold work shock applications. This datasheet provides information on composition, physical properties, hardness, and elasticity as well as fracture toughness. It also includes information on heat treating, machining, and joining. Filing Code: TS-543. Producer or source: Crucible Service Centers.

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