scholarly journals Potential utility of targeted Nanopore sequencing for improving etiologic diagnosis of bacterial and fungal respiratory infection

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Wai Sing Chan ◽  
Chun Hang Au ◽  
Sau Man Leung ◽  
Dona N. Ho ◽  
Elaine Yue Ling Wong ◽  
...  
Keyword(s):  
Respiratory Infection ◽  
Nanopore Sequencing ◽  
Potential Utility ◽  
Etiologic Diagnosis

Potential utility of metagenomic sequencing for improving etiologic diagnosis of infective endocarditis

2019 ◽  
Vol 15 (6) ◽  
pp. 411-424
Author(s):  
Wai-Sing Chan ◽  
Chun-Hang Au ◽  
Henry Chi-Ming Leung ◽  
Dona N Ho ◽  
Dinghua Li ◽  
...  
Keyword(s):  
Infective Endocarditis ◽  
Heart Valves ◽  
Illumina Miseq ◽  
Metagenomic Sequencing ◽  
Potential Utility ◽  
Bacterial Genomes ◽  
Bartonella Quintana ◽  
Replacement Surgery ◽  
Valvular Replacement ◽  
Etiologic Diagnosis

Aim: To explore potential utility of metagenomic sequencing for improving etiologic diagnosis of infective endocarditis (IE) caused by fastidious bacteria. Materials & methods: Plasma and heart valves of two patients, who were diagnosed with IE caused by Bartonella quintana and Propionibacterium species, were sequenced by using Illumina MiSeq and Nanopore MinION. Results: For patient 1, B. quintana was detected in the plasma pool collected 4 days before valvular replacement surgery. For patient 2, Propionibacterium sp. oral taxon 193 was detected in the plasma sample collected on hospital day 1. Nearly complete bacterial genomes (>98%) were retrieved from resected heart valves of both patients, enabling detection of antibiotic resistance-associated features. Real-time sequencing of heart valves identified both pathogens within the first 16 min of sequencing runs. Conclusion: Metagenomic sequencing may be a helpful supplement to IE diagnostic workflow, especially when conventional tests fail to yield a diagnosis.

Diagnosis of Atypical Pathogens in Patients Hospitalized with Community-acquired Respiratory Infection

2004 ◽  
Vol 36 (4) ◽  
pp. 269-273 ◽  
Author(s):  
Peter M. Schneeberger ◽  
J. Wendeline Dorigo-zetsma ◽  
Anneke van der Zee ◽  
Marion van Bon ◽  
Jean-louis van Opstal
Keyword(s):  
Respiratory Infection ◽  
Atypical Pathogens

Promoting respiratory infection control practices in primary care: Primary care/public health collaboration

2005 ◽  
Author(s):  
William Hogg ◽  
Patricia Houston ◽  
Carmel Martin ◽  
Raphael Saginur ◽  
Adriana Newbury ◽  
...  
Keyword(s):  
Public Health ◽  
Primary Care ◽  
Infection Control ◽  
Respiratory Infection

Influence of Previous Respiratory Infection on the Association between Respiratory Syncytial Virus Hospitalization and Childhood Asthma

2020 ◽  
Author(s):  
Richard Thwaites ◽  
Jonathan Coutts ◽  
John Fullarton ◽  
ElizaBeth Grubb ◽  
Carole Morris ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Respiratory Infection ◽  
Childhood Asthma ◽  
Syncytial Virus

Respiratory Complications in Outpatient Endoscopy with Endoscopist-Directed Sedation

2014 ◽  
Vol 23 (3) ◽  
pp. 255-259 ◽  
Author(s):  
Kilian Friedrich ◽  
Sabine G. Scholl ◽  
Sebastian Beck ◽  
Daniel Gotthardt ◽  
Wolfgang Stremmel ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Respiratory Infection ◽  
Endoscopic Procedure ◽  
Shortness Of Breath ◽  
Respiratory Complications ◽  
Endoscopic Procedures ◽  
Increased Risk ◽  
High Incidence ◽  
Respiratory Complaints

Background & Aims: Respiratory complications represent an important adverse event of endoscopic procedures. We screened for respiratory complications after endoscopic procedures using a questionnaire and followed-up patients suggestive of respiratory infection.Method: In this prospective observational, multicenter study performed in Outpatient practices of gastroenterology we investigated 15,690 patients by questionnaires administered 24 hours after the endoscopic procedure.Results: 832 of the 15,690 patients stated at least one respiratory symptom after the endoscopic procedure: 829 patients reported coughing (5.28%), 23 fever (0.15%) and 116 shortness of breath (SOB, 0.74%); 130 of the 832 patients showed at least two concomitant respiratory symptoms (107 coughing + SOB, 17 coughing + fever, 6 coughing + coexisting fever + SOB) and 126 patients were followed-up to assess their respiratory complaints. Twenty-nine patients (follow-up: 22.31%, whole sample: 0.18%) reported signs of clinically evident respiratory infection and 15 patients (follow-up: 11.54%; whole sample: 0.1%) received therefore antibiotic treatment. Coughing or vomiting during the endoscopic procedure resulted in a 156.12-fold increased risk of respiratory complications (95% CI: 67.44 - 361.40) and 520.87-fold increased risk of requiring antibiotic treatment (95% CI: 178.01 - 1524.05). All patients of the follow-up sample who coughed or vomited during endoscopy developed clinically evident signs of respiratory infection and required antibiotic treatment while this occurred in a significantly lower proportion of patients without these symptoms (17.1% and 5.1%, respectively).Conclusions: We demonstrated that respiratory complications following endoscopic sedation are of comparably high incidence and we identified major predictors of aspiration pneumonia which could influence future surveillance strategies after endoscopic procedures.

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