SUMMARYThe transmission of parasites can be influenced by their co-occurrence with other parasites, in some cases increasing or reducing transmission.Trypanosoma cruzi, aetiologic agent of Chagas disease, often co-occurs withTrypanosoma rangeli, a parasite not pathogenic for mammal hosts. Both parasites can reduce the fitness of their insect vectors (the triatomine bugs; Hemiptera: Reduviidae), withT. rangelibeing more pathogenic for some species. Here, we study the prevalence ofT. cruziandT. rangeliin the triatomineRhodnius pallescensacross a heterogeneously transformed landscape in Panamá. We found that singleT. rangeliinfections were more common in contiguously forested habitats, while singleT. cruziinfections predominated in anthropogenically disturbed habitats.Trypanosoma cruzi–T. rangelico-infections were more common in contiguous forests and in peridomiciliary areas. Furthermore, adult insects were more likely to be co-infected than nymphs. Our results suggest that human-mediated landscape transformation might have increased the predominance of single infections withT. cruziwithin vectors. An important mechanism driving changes in trypanosome infection patterns in triatomines at a landscape scale includes alterations in host species composition that may vary with different degrees of deforestation. Trypanosome co-infection may also confer a survival advantage forR. pallescensto and/or throughout adulthood.